• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.326-335
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• 2015

Citrus is an economically important fruit tree with the largest amount of fruit production in the world. It provides important nutrition such as vitamin C and other health-promoting compounds including its unique flavonoids for human health. However, it is classified into the most difficult crops to develop new cultivars through conventional breeding approaches due to its long juvenility and some unique reproductive biological features such as gamete sterility, nucellar embryony, and high level of heterozygosity. Due to global warming and changes in consumer trends, establishing a systematic and efficient breeding programs is highly required for sustainable production of high quality fruits and diversification of cultivars. Recently, reference genome sequences of sweet orange and clementine mandarin have been released. Based on the reference whole-genome sequences, comparative genomics, reference-guided resequencing, and genotyping-by-sequencing for various citrus cultivars and crosses could be performed for the advance of functional genomics and development of traits-related molecular markers. In addition, a full understanding of gene function and gene co-expression networks can be provided through combined analysis of various transcriptome data. Analytic information on whole-genome and transcriptome will provide massive data on polymorphic molecular markers such as SNP, INDEL, and SSR, suggesting that it is possible to construct integrated maps and high-density genetic maps as well as physical maps. In the near future, integrated maps will be useful for map-based precise cloning of genes that are specific to citrus with major agronomic traits to facilitate rapid and efficient marker-assisted selection.

• Journal of Animal Science and Technology
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• v.50 no.5
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• pp.633-640
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• 2008

With a double mismatch primer set designed for amplifying the modified DNA sequence fragments, bovine melanocortin-1 receptor(MC1R) gene encoded in Extension locus which plays a critical role in coat color development was analyzed using polymerase chain reaction mediated restriction fragment length polymorphism(PCR-RFLP). Amplified PCR fragments were successfully discriminated with combining the MspI- and AluI-RFLP into three major alleles(ED, E+, and e), directly related to bovine coat color phenotypes. The genotyping results showed that Jeju black cattle contained three MC1R alleles, but yellowish-red colored Hanwoo and bridle colored Korean Brindle cattle did not contained the dominant black allele ED. However, two dominant black-colored cattle breeds, Holstein and Angus, contained the ED allele over 96% in frequency. Hanwoo×Holstein F1 and Hanwoo×Angus F1 crossbred calves showed ED/e MC1R genotypes, and uniformly black coat color. the results suggested that this MC1R genotyping method be useful in allele discrimination for bovine MC1R gene which used for breed classification and characterization, as one of the important genetic markers, using combination of MspI- and AluI-RFLP for modified PCR product amplified with a newly designed double mismatch primer set.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.13 no.1
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• pp.1-10
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• 2018

The importance of entrepreneurship has been emphasized recently from academia and government officials and studies trying to investigate entrepreneurial alertness as core competence of entrepreneurial mindset in the research field of entrepreneurship continue. In domestic academic research, research on entrepreneurial alertness has not yet been conducted in earnest, unlike the active flow of research overseas. This paper aims to investigate what factors influence entrepreneurial alertness in the Korean environment, we conducted an empirical analysis through a questionnaire survey for CEOs of small and medium enterprises in Seoul, Gyeonggi province. In this study, we selected prior knowledge of markets and technology, positive attitude, social network, number of books per month as independent variables on entrepreneurial agility based on Ardichvili's Alertness model, and as control variables respondents' Demographic characteristics, such as gender, age, founding career were selected. According to the results of the empirical analysis, prior knowledge of markets and technology, positive attitudes, number of books per month as independent variables, showed positive influence on entrepreneurial alertness, showing statistical significance. However, the social network picked up measured variables by the number of regular meetings which are quantitative indicators, but found that there is no statistically significant effect on entrepreneurial alertness. Despite several limitations of this research, we investigate what factors influence entrepreneurial alertness through empirical research on entrepreneurial alertness impact factors that have not been explained in the domestic academic research. Although you saw it, it can be said that there is academic contribution.

• Journal of fish pathology
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• v.20 no.2
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• pp.109-117
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• 2007

This study was performed to identity non hemolytic streptococcus from cultured flounder (Paralichthys olivaceus) with Streptococcosis in the Jeju island. The result of BIOLOGTM test was Streptococcus uberis that simility of 0.5 and 98% identified in MicroLogTM system (Release 4.05). Carbohydrate utility pattern was dextrin, N-acetyl-D-glucosamine, arbutin, maltose, maltotriose, D-cellobiose, D-fructose, D-mannose, α-D-glucose, D-mannitol, β-methyl D-glucoside, salicin, sucrose, D-trehalose, pruvatic acid methyl ester, mono-methyl succinate, glycerol. In addition hemolysis test for S. parauberis and were S. iniae hemolysis in BAP (Blood agar plate). Antibiotic test for S. parauberis were Ampicillin, Amoxicillin and Fluoroquinolone sensitivity. Mutiplex PCR assay were detected S. pauberis (718 bp), S. iniae (870 bp) L. garviae (1,100 bp). Dectected S. parauberis (718 bp) were result of 16S rRNA sequence identified with S. parauberis (Gene bank accession number X89967). All isolated S. parauberis that with bouned by one group. The result were S. pauberis that γ-hemolytic chain form cocci and negative reaction of catalase, Multiplex PCR assay were 718 bp amplicon size.

• The Journal of Engineering Geology
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• v.30 no.3
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• pp.327-345
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• 2020

The effects of ground vibration on lava tubes during construction were studied to aid design of management and preservation measures for lava tubes. Ground conditions were assessed by RMR (Rock mass rating) and Q-system classifications for the Geomunoreum lava tubes, and vibration velocity was measured during in situ blasting tests in the Manjanggul and Yongcheondonggul lava tubes. Results indicate that the higher the rock quality, the greater the effect of vibration, although there is no clear linear relationship due to ground heterogeneity. A relationship derived between vibration velocity (PPV) and intensity (dB(V)) on the basis of blasting tests indicates that a vibration level of < 0.285 cm/sec meets the regulatory limit of 0.371 cm/sec and 65 dB(V) during daytime, and 0.285 cm/sec and 60 dB(V) during night. For blasting vibrations, square- and cube-root scaled distances are linearly correlated, with R² ≥ 0.76. On the basis of this correlation, explosive-charge weights meeting the 0.2 cm/sec vibration criterion for cultural heritage were estimated to be 2.88 kg at 50 m distance, and 11.52 kg at 100 m.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.1
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• pp.45-52
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• 2007

This study was conducted from 2003 to 2005 at crop field of National Institute of Subtropical Agriculture, RDA, in Jeju. The treatment consisted of chemical fertilizer 100%(CF 100%, T1), cattle manure 100%(CM 100%, T2), CF 50%+CM 50%(T3), CF 25%+CM 75%(T4), CM 100%(1st yr.)+CF 100%(2nd yr,)+CM(3rd yr)(T5), CM 100%(1st yr.)+CF 100%(2nd yr.)+CF(3rd yr.)(T6). The highest substitution effect of chemical fertilizer for cattle manure indicated that the highest yield(Avg. 11,169 kg/ha) obtained from CM 100%(1 yr.)+CF 100%(2 yr.) treatment. The forage yield of CM 100%(1 yr.)+CF 100%(2 yr.) was 4% more than CM 100% application whereas was 3% less than CF 100% application. Changes of physical and chemical properties of soils for applications of CF 100% and CM 100% was clearly in cattle manure application. Especially, cattle manure application in pasture were superior to chemical fertilizer application with respect to organic matter(OM, %), total nitrogen(T-N, %), and avaliable phosphorous($P_2O_5$, %) contents of soils. In this present study, cattle manure application recorded slightly lower forage yields than CF 100% application but the combined use of cattle manure and chemical fertilizer was attribute to reduction of chemical fertilizer application rate as well as maintaining soil health on long-term basis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.7
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• pp.453-461
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• 2020

The purpose of this study was to investigate the mediating effects of young children's playfulness between the children's peer competence and their mothers' play beliefs. For this study, 160 mothers and 160 children aged 4-5 years who were in kindergarten and daycare centers in Area J were selected. The collected data were analyzed based on correlation and mediation using SPSS Statistics. The research results are as follows. First, as a result of analyzing the relationship between the young children's peer competence and playfulness and their mothers' play beliefs, there was a statistically significant positive correlation between a mother's play-support beliefs and their children's peer competence and playfulness. On the other hand, the relationship between a mother's learning-support beliefs and her child's peer competence and playfulness was not significant. There was a significant positive correlation between playfulness and peer competence in the young children. Second, as a result of verifying the mediating effect of the children's playfulness in the relationship between their mothers' play-support belief and the children's peer competence, we found that playfulness has a partial mediating role.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.7
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• pp.224-236
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• 2017

This study analyzed the characteristics of traffic accidents among elderly drivers according to area type in Gyeonggido(urban areas, urban-rural complex areas, and rural areas). Traffic accidents caused by unsafe driving are common in all three types of areas. In urban areas and urban-rural complex areas, traffic accidents were more frequent due to traffic violations and intersection accidents, while rural areas were more affected by central lines. Urban areas and urban-rural complex areas require simpler signal exposures, such as a simple straight line, left turn, or straight line and left turn signal rather than complex signal operations, such as overlaps in a signal operation. Improvement of non-signal intersections and unprotected traffic signal processing is also needed. In order to prevent the intrusion of the central line in rural areas, the center line guard rail should be prevented from penetrating the center line, and the influence of the vehicle headlamp should be reduced in the opposite direction. It is necessary to improve the visibility by using fluorescent letters to enlarge the font size of traffic signs and road signs, particularly to increase visibility at night and prevent traffic accidents at sunset. Minimal, simplified should be provided rather than complex phrases and information.

• Journal of Distribution Science
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• v.11 no.5
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• pp.43-53
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• 2013

Purpose - The purpose of this study is to analyze the effectiveness of customer relationship management (CRM) support policies for facilitating traditional markets, especially with respect to customer acquisition and maintenance, and to investigate the factors affecting CRM. Research design, data, and methodology - We analyzed the CRM efficiency of traditional markets in 16 cities and provinces in Korea on the basis of DEA analysis and Malmquist productivity analysis. The DEA model calculates a ratio of the weighted mean of various inputs to the weighted mean of various outputs and measures the efficiency of a specific decision making unit (DMU), which is compared to the reference group that has a similar input-output structure. The input variables are coupon, event, parcel service, premiums, while is the number of customers per day. Further, through regression analysis, we analyzed CRM-related factors affecting traditional markets' customer appeal and revenue growth. Results - We obtained the results of the efficiency of traditional markets in 16 provinces. The traditional markets in Seoul, Busan, and Jeju were found to be efficient in a model CCR that used the number of customers per day as an output variable, while Chungbuk, Jeonbuk Province, and According to the results of the DEA analysis and Malmquist productivity analysis, large cities such as Seoul, Busan, and Jeju showed efficiency in CRM-related investment businesses in traditional markets for attracting customers. The Malmquist analysis results confirmed that the productivity of traditional markets increased from 2008 to 2010. The results of the regression analysis revealed that the "customer acquisition/maintenance factor" and the "offering of customer convenience facility factor" were significant to the daily average number of customers, which is a dependent variable. The results of the test with the mediating variable, "number of customers," and the final dependent variable, "sales revenue," were rejected. However, the variable "customer acquisition /maintenance" was found to affect sales revenue positively. Conclusions - It is necessary to enhance the business not only for promotional activities to attract customers, but also to strengthen customer relationships among CRM businesses, such as through the management of key customers. The regression analysis results showed that CRM businesses have yet to produce an increase in sales revenues in traditional markets. Therefore, to help customers who visit traditional markets to keep buying products, it is necessary to prepare various investment methods and provide support to improve "customer loyalty." This study has a limitation in terms of CRM-related statistics. Therefore, in the future, it is necessary to conduct a survey of customers who use traditional markets to analyze the markets by type and size as well as the CRM-related factors. Based on the analysis, we will try to perform a variety of statistical analyses, including structural equations.

• The Korean Journal of Emergency Medical Services
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• v.6 no.1
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• pp.153-168
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• 2002

Since 1992, conventional fire fighting businesses have been converted into a metropolitan autonomous fire fighting system to be ready for a variety of disasters. However, the corresponding investment has been overlapped due to the non-integration of businesses to prevent any potential disasters, and a series of collaborative systems have been not functioning so well. In the meantime, our fire fighting sector has been trying to set up its own clean and faithful position by abolishing any inconvenient system or outsourcing private sectors, and expanded its work scope from conventional fire fighting even to rescue and emergency works. While focusing on handling disaster, the fire fighting sector has been widely trusted and reliable throughtout our nation. Moreover, our fire fighting sector has secured nation wide mobile organizations, technical personnel by field, special equipments and independent communication network. In addition, the fire fighting sector has knowledges, expertise and capabilities required for managing disasters, while in charge of almost every disaster management works including fire, explosion, collapse, disaster and flood. It becomes an organization for comprehensive disaster management under an absolute national trust, which is based on the system for prevention, preparation and countermeasure against a variety of disasters. Thus, our fire fighting sector must make many efforts and try to modernize conventional education and training. The ways to facilitate rescue and emergency works may include the nurture of technical fire fighting personnel along with modernized equipments, the reinforcement of rescue and emergency education, the facilitation of operating civil defense corps, the facilitation of operating volunteer fire fighting corps, the better arrangement of 119 briefing room for public healthcare in provincial offices, the sterilization of rescue instruments and equipments the better repair education for emergency rescue member, the establishment of regional emergency assistant system and the expansion of fire fighting personnel and equipments. In terms of reinforcing the functions and services of rescue 119 and emergency corps, we must review the following considerations: Building up security system along with operational expansion, building up a system for emergency medical treatment, building up a comprehensive information management system for rescue and emergency, constructing a provincial safety museum and so forth. For the ways to better the works of rescue 119 we can review the following considerations : Improving the education for fire fighting training corps under Jeju Provincial Fire and Disaster Management Department, providing rescue members with more opportunities for clinical practices, enhancing the morale of rescue members, installing a comprehensive briefing room for emergency rescue members, building up medical networks along with reasonable policies for information service, operating the consulting system for rescue 119 and so on. If these requirements are met, it is expected that the fire fighting departments in Jeju province can cope with every accident and disaster a little more rapidly and quickly in compliance with local needs, so that they can keep their own position as a public fire fighting organization which may be trusted by the public.