• Plant Biotechnology Reports
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• 제5권1호
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• pp.27-34
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• 2011

Jasmonates control diverse plant developmental processes, such as seed germination, flower, fruit and seed development, senescence and tuberization in potato. To understand the role of methyl jasmonate (MeJA) in potato tuberization, the Arabidopsis JMT gene encoding jasmonic acid carboxyl methyltransferase was constitutively overexpressed in transgenic potato plants. Increases in tuber yield and size as well as in vitro tuberization frequency were observed in transgenic plants. These were correlated with JMT mRNA level-- the higher expression level, the higher the tuber yield and size. The levels of jasmonic acid (JA), MeJA and tuberonic acid (TA) were also higher than those in control plants. Transgenic plants also exhibited higher expression of jasmonate-responsive genes such as those for allene oxide cyclase (AOC) and proteinase inhibitor II (PINII). These results indicate that JMT overexpression induces jasmonate biosynthesis genes and thus JA and TA pools in transgenic potatoes. This results in enhanced tuber yield and size in transgenic potato plants.

• 생약학회지
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• 제31권2호
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• pp.235-239
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• 2000

The effects of several compounds related to signal transduction cascade were determined to induce the production of alizarin and purpurin in the hairy root culture system of Rubia cordifolia var. pratensis. It was found that out of five tested compounds jasmonic acid(1 mg/l) and methyl jasmonate(1 mg/l) stimulated strongly the biosynthesis of the pigments while linolenic acid(1 mg/l) induced no significant increase of the product. Yeast extract(600 mg/l) and arachidonic acid(1 mg/l) showed relatively mild inducing effects on production of alizarin. The effects of jasmonic acid and methyl jasmonate were reduced by treatment with cycloheximide(2.8 mg/l).

• Plant Resources
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• 제1권1호
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• pp.49-52
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• 1998

This study confirmed that the initiation time of tuberization was well consistent with the cativation time of JA. The consistency was also cinfirmed in the tuberization of yam plants under the altered condition of natural day length. The final yield of JA from 500g fresh leaves was $89.3{\mu}g$.

• Journal of Applied Biological Chemistry
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• 제44권3호
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• pp.119-124
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• 2001

NTR1 gene of Brassica campestris L. ssp. perkinensis encodes a floral nectary-specific methyltransferase. In this study, the NTR1 cDNA was expressed in E. coli to examine the enzymatic characteristics of the protein product. The GST-NTR1 fusion protein was purified to near homogeneity, showing that the size of NTR1 was 44 kDa. The protein reacted specifically with jasmonic acid (JA), consuming methyl group from S-adenosyl-L-methionine (SAM). GC-MS analysis revealed that the compound produced was authentic methyl jasmonate (MeJA), suggesting that NTR1 is an S-adenosyl-L-methionine: jasmonic acid carboxyl methyltransferase. Km values of NTR1 for JA and SAM were 38.0 and $6.4{\mu}M$, respectively. Optimal activity of the NTR1 was observed at $20^{\circ}C$, pH 7.5, in the presence of 100-150 mM KCl. Thus, kinetic properties, thermal characteristics, optimal pH, and ion-dependency of the NTR1 activity were almost identical to those of Arabidopsis JA methyltransferase JMT, indicating that these two proteins are orthologues of each other.

• 미생물학회지
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• 제42권3호
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• pp.210-215
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• 2006

Diplodia gossypina ATCC 10936에 의해 생산되는 카이럴(+)-jasmonic acid(JA)는 생물학적으로 가장 활성이 높은 형태로 식품성장 hormone으로서 뿐만 아니라 상업적으로 jasmingid 생산에 이용되는 중요한 물질중 하나이다. (+)-JA의 대량 생산을 위한 공정 개발을 위해, D. gossypina ATCC10936을 이용하여 (+)-JA 생산을 위한 최적배양 조건을 조사하였다. 최적 탄소원으로는 fructose와 glucose로 확인되었으며 질소원으로는 $NaNO_3$ (+)-JA를 생산에 가장 적합하였다. (+)-JA 생산의 최적 온도와 회전 교반수는 $28^{\circ}C$와 200 rpm으로 나타났다. 균사체의 균체량은 PDMYS 배지에서 최대로 증가하였으나, (+)-JA 생산은 SM 배지에서 최대 600 mg/L가 생산되었다.

• The Plant Pathology Journal
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• 제27권1호
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• pp.53-58
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• 2011

Systemic acquired resistance is a form of inducible resistance that is triggered in systemic healthy tissues of local-infected plants. Several candidate signaling molecules emerged in the past two years, including the methylated derivatives of well-known defense hormones salicylic acid (SA) and jasmonic acid (JA). In our present study, the symptom on Cucumber mosaic virus (CMV) infected Arabidopsis leaves in 0.1 mM SA or 0.06 mM JA pre-treated plants was lighter (less reactive oxygen species accumulation and less oxidative damages) than that of the control group. JA followed by SA (JA${\rightarrow}$SA) had the highest inhibitory efficiency to CMV replication, higher than JA and SA simultaneous co-pretreatment (JA+SA), and higher than a JA or a SA single pretreatment. The crosstalk between the two hormones was further investigated at the transcriptional levels of pathogenesis-related genes. The time-course measurement showed JA might play a more important role in the interaction between JA and SA.

• 한국산림과학회지
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• 제96권5호
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• pp.539-542
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• 2007

This study was carried out to investigate the impacts of jasmonic acid (JA) on adventitious root culture of Eleutherococcus senticosus. Adventitious root of E. senticosus were treated with jasmonic acid (JA) and cultured for 30 days. JA inhibited the root growth but increased eleutherosides accumulation, total phenolic contents and antioxidant activity. Among various concentrations of JA, 1.0 mg/L JA increased the total phenolic contents in E. senticosus adventitious root to $39.81{\mu}g/g$, about 2.6 times higher than that of the control. Consequently, high accumulation of total phenolic contents led to increase the antioxidant activity to 82.41%. The antioxidant activity of control was 37.89% at $2500{\mu}g/mL$. A linear correlation ($R^2$ = 0.9937, 0.9648 and 0.9883) was also shown between antioxidant activity (at 1250, 1875, and $2500{\mu}g/mL$) and total phenolic contents of adventitious root of E. senticosus.

• Journal of Plant Biotechnology
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• 제5권2호
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• pp.95-99
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• 2003

The leaf segments of garlic (Allium sativum L.) were cultured in vitro and determined optimal concentration of plant growth regulators and sugars for callus induction, multiple shoots regeneration and in vitro bulblet formation. Highest yield of callus was observed in the leaf segment culture on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-D, 30 g/L sucrose and 8 g/L agar. Regeneration rate of multiple shoots from callus was high in the MS medium supplemented with kinetin 3.0 + NAA 3.0 mg/L or SA 1.0 + NAA 3.0 mg/L, containing 30 g/L sucrose. High rate of bulblet formation was observed as the concentration of jasmonic acid increased from 0.5 to 2.0 mg/L in medium, whereas addition of gibberellic acid significantly suppressed bulblet formation. The rate of in vitro bulbing was as high as $96\%$ in MS medium supplemented with 2.0 mg/L jasmonic acid and 120 g/l sucrose after two month culture at $25{\pm}1^{\circ}C$ under 16 hours day light.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제4권4호
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• pp.231-240
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• 2000

Effects of Jasmonic Acid and Wounding on Polyphenol Oxidase Activity in Senescing Tomato Leaves The effects of jasmonic acid(JA) and wounding on polyphenol oxidase(PPO) during leaf senescence was investigated by measuring the PPO activity in detached tomato(Lycopersicon esculentum Mill.) leaves of two-week-old seedlings. The PPO activity in the detached senescing leaves increased significantly in the dark. The leaf segments responded to the application of JA with accelerated senescence, as indicated by the loss of chlorophyll and rapid increase in the PPO activity. The senescence-promoting action of JA differed in the light and dark. Wounding the detached senescing leaves by scraping surface segments or making punctures with needles considerably delayed the loss of chlorophyll and had a significant effect on the PPO activity, the amounts of which were roughly proportional to the intensity of the wounding. In the dark, the combination of wounding plus JA resulted in stable levels of chlorophyll and PPO. JA and ABA acted similarly in both unwounded and wounded leaves, however, the amount of chlorophyll and PPO in the wounded segments was always higher than in the respective controls. JA was found to eliminate the senescence-retarding action of benzyladenine. In a histochemical localization test, the PPO activity was found to be localized in the cell walls of the parenchyma tissue, thereby indicating moderate cytoplasmic reactions. In the JA-treated plants, the PPO activity was intense in the cells of the cortex and phloem parenchyma. Accordingly, based on these observations it would appear that PPO is a component of a defense response maker, whereas JA plays an integral role in the intracellular signal transduction involved in inducible defense mechanisms.

• KSBB Journal
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• 제15권5호
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• pp.428-433
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• 2000

희수 (Camptotheca acuminata) 현닥세포배양에서 최근 항암 제로서 관심을 팔고있는 camptothecin을 얻기 위하여 연구를 수행하였다. 현탁세포배양에서 exponential phase의 최고 생장 속도는 $0.269day^{-1}$이었으며 배가시간이 2.58일이었다. Camptothecin 은 배양초기에 생성되지 않고 exponential phase 말기부터 서서히 생성되어 stationary phase 초기에 최고를 나타내고 이후 급격히 감소하였다. Camptothecin의 생산을 촉진하기 위하여 elicitor를 사F용하였다. 여러 elicitor 중 jasmonic acid와 cellulase 가 camptothecin의 생성을 증가시켰다, Elicitor는 접종과 동시 에 투여 했을 때 가장 효과가 좋았으며, jasmonic acid와 cellulase 의 combination으로 효과가 더욱 증진했는데 대조구에 비하여 약 20배 정도 증가한 $20.42{\times}10^{-3}mg/\ell$ 의 camptothecin이 생 성되었다. 이것은 생산량을 증가시킨 것뿐만 아니라 생산기 간도 약 1/4로 단축시키는 결과를 얻을 수 있었다.