• Mycobiology
• /
• v.41 no.1
• /
• pp.59-62
• /
• 2013

Japanese pine sawyer beetle (Monochamus alternatus) is an economically important pest in coniferous trees. Ophiostoma ips was isolated from the beetle and identified based on analysis of morphological properties and the ${\beta}$-tubulin gene sequence. The fungus easily produced perithecia with a long neck on malt extract agar and its ascospores were rectangular shaped. This is first report of Ophiostoma species associated with the pinewood nematode vector beetle in Korea.

• Mycobiology
• /
• v.35 no.3
• /
• pp.159-161
• /
• 2007

Pine tree death caused by pine wood nematode(PWN) involves phoretic relationships between PWN and its vector Japanese pine sawyer beetle(JPS). In an effort to understand the diversity of fungi involved in PWN life cycle, a total of 176 fungal isolates were collected from PWNs, adults and larvae of JPS, PWN-diseased Japanese black pine that was cut down in 2005 at Jinju, Korea. Based on microscopic observation and colony morphology, and sequence analysis of the ITS rDNA, the fungal isolates were identified at the level of genus. Three genera including Mucor, Ophiostoma, and Penicillium were identified from PWN. Two genera of Ophiostoma and Penicillium were discovered from JPS larvae. Frpm JPS adult beetles, nine genera of Aspergillus, Gibberalla, Hypocrea, Irpex, Leptosphaeria, Ophiostoma, Penicillium, and Plectosphaerella and unknown basidio-mycetes were found. Ten genera from PWN-infected weed were confirmed as Bionectria, Botrytis, Camarops, Fusarium, Hypocrea, Nectria, Mucor, Ophiostoma, Penicillium, and Trichoderma. Penicillium and Ophiostoma were commonly distributed on PWN and its vector and host. This is first report of the fungi associated with PWN and its vector and host in Korea.

• Korean journal of applied entomology
• /
• v.48 no.2
• /
• pp.189-195
• /
• 2009

This study was performed to investigate the emergence timing and daily emergence from the different tree species logs (Korean white pine, Pine, and Japanese larch) at Cheongwon, Chungcheongbuk-do from 2006 to 2008. Pine sawyer adults began to emerge on early May (2${\sim}$10th day) and finished on mid-June (4${\sim}$20th day) in the Korean white pine and pine logs. However, pine sawyer adults began to emerge on mid-May (13th day) and finished late May (27th day) in Japanese larch logs, Japanese larch logs shows shorter emergence timing of pine sawyer adult compare to those of other two tree species. Emergence timing shows no difference between males and females. Sex ratios of emerged adult in 2007 were 0.55 from Korean white pine, 0.46 from Pine, and 0.59 from Japanese larch; it shows no difference among tree species. Female and male adults emerged throughout 24 hrs; 22.8% of the total adults emerged from 12${\sim}$14:00 (22.8%) and adults emerged lowest from 06${\sim}$08:00. Of the adults emerged from the logs, 98.9% was univoltine and the rest was biennial which emerged next year.

• Korean journal of applied entomology
• /
• v.49 no.3
• /
• pp.211-218
• /
• 2010

Cuticular hydrocarbons (CHCs) of the pine sawyer (Monochamus saltuarius), Japanese pine sawyer (M. alternatus) and oak longicorn beetle (Moechotypa diphysis) were analyzed by GC, GC-MS and compared. Monochamus beetles are typical vectors of pine wilt disease but Moechotypa diphysis, which belongs to the same family, is not. They possess different CHCs in carbon number: 23-25 in M. saltuarius, 25-32 in M. alternatus, and 23-29 in M. diphysis. In comparison to inter-species, these three species of adult beetles have different numbers and chains of constituents of CHCs. In comparison between male and female in intra-species, the quantities of CHCs show the difference but constituents are not. Major constituent of M. saltuarius were analyzed as n-pentacosane > n-nonacosane > n-heptacosane; those of M. alternatus were n-nonacosene > n-pentacosane > n-nonacosane; and those of M. diphysis were n-heptacosane > 13-methylheptacosane > 3-methylheptacosane. From the body surface, most saturated carbohydrates of 3 species beetles are composed of n-alkane (40.2 - 65.7%) and followed by olefines > monomethylalkanes that one or two double bonds in M. saltuarius and M. alternatus. Otherwise, M. diphysis have the difference in order of monomethylalkanes > olefins.