• Asian-Australasian Journal of Animal Sciences
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• 제23권9호
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• pp.1137-1144
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• 2010

In this study, to examine genetic variability within a breed and genetic relationships between populations/breeds, we genotyped 606 birds from seven Japanese native chicken breeds at seven polymorphic loci of egg white proteins and compared those with Asian native chicken populations and commercial breeds. Genotyping of the Japanese native breeds showed that ovalbumin, two ovoglobulins and ovotransferrin were polymorphic, but ovomacroglobulin, ovoflavoprotein and lysozyme were monomorphic. The proportion of polymorphic loci ($P_{poly}$) and average heterozygosity ($\bar{H}$) within a population ranged from 0.286 to 0.429 and from 0.085 to 0.158, respectively. The coefficient of gene differentiation ($G_{ST}$) was 0.250 in the Japanese native chicken breeds. This estimate was higher than that of Asian native chicken populations ($G_{ST}$ = 0.083) and of commercial breeds ($G_{ST}$ = 0.169). Dendrogram and PCA plot showed that Satsuma-dori, Jitokko, Amakusa-daio and Hinai-dori were closely related to each other and grouped into Asian native chickens and that Tsushima-jidori, Nagoya and Chan (Utaichan) were ramified far from other Japanese native chicken breeds. The egg white protein polymorphisms demonstrated that the population differentiation of the seven Japanese native chicken breeds was relatively large.

• 한국조리학회지
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• 제9권3호
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• pp.102-113
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• 2003

This study was conducted to investigate Korean soy sauces and Japanese soy sauces for their flavor and taste when they are used for Teriyaki sauce preparation. The results showed the followings; 1. The panelists liked more naturally brewed soy sauce (NBS) than mixed (NBS + acid-hydrolyzed) soy sauces, especially S company's NBS, 501S and Japanese K company's koikuchi NBS were preferred sauces. 2. The preference of Chicken Teriyaki preparation were also appeared to be the highest with S company's NBS, 501S and Japanese K company's koikuchi NBS. Both of them are naturally brewed soy sauces. 3. Chicken Teriyaki Sauce's preference were also high that made from the highly preferred soy sauces, therefore, it would be the better selection with the highly preferred soy sauces for the Chicken Teriyaki preparation. 4. There were no significant difference in preferences between imported NBS and domestic NBS, so it would be good to use Korean NBS for Chicken Teriyaki preparation.

• 한국축산식품학회지
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• 제21권4호
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• pp.307-313
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• 2001

본 연구는 일본 동경지역에서 시판되고 있는 한국산과 각국산 계육의 미생물학적, 물리화학적 품질특성을 비교분석하였다. 시료는 일본 동경 지역 판매점에서 냉동저장(-2$0^{\circ}C$$\pm$1) 상태에서 시판되고 있는 각국산 계육의 가슴육과 다리육을 각각 구입하여 일본 낙농학원대학 식품과학과 연구실에서 실험이 진행되었다. 가슴육은 한국산과 일본산, 중국산이 비교분석되었고, 다리육은 한국산 일본산, 중국산, 미국산, 태국산이 각각 비교분석되었다. 가슴육의 일반성분의 경우 조지방의 함량은 한국산 계육의 경우 가장 낮은 함량을 나타내었고, 조단백질과 조회분(P<0.01)은 중국산 닭고기가 가장 낮은 함량을 나타내었으나, 수분 함량은 가장 높은 수준을 보였으며 P<0.001수준에서 유의적인 차이를 나타내었다. 가슴육의 물리화학적 특성에서는 보수성의 경우 일본산 닭고기가 가장 높게 나타났으나, 지방의 산화정도를 나타내는 TBA값이 한국산과 중국산에 비하여 높게 나타났다. 가슴육의 미생물수준은 각 국산간에 총균수, 대장균군 모두 고도의 유의적인 차이를 나타내었으며(P<0.001), 총균수의 경우 중국산이 가장 높게 나타났으며, 한국산 닭고기 가슴육은 가장 높은 대장균군을 나타내었다. 다리육의 일반성분 경우 수분은 미국산과 태국산이 높게 나타났으며(P<0.001), 조지방 함량은 미국산 닭고기 다리육에서 가장 높게 나타났다(P<0.001). 닭고기 다리육의 명도(L value)와 황색도(b value)는 모두 고도의 유의적인 차이를 나타내었으며(P<0.001), 명도의 경우 태국산과 일본산이 가장 높은 수준을 나타내는 반면 황색도에서는 일본산의 경우 가장 낮은 수준을 나타내었다. 닭고기는 다리육의 미생물수준은 총균수와 대장균군 모두 고도의 유의적인 차이를 나타내었다(O<0.001). 총 균수는 태국산이 $10^{5}$ 이상의 수준으로 가장 높게 나타났고, 그 외 다른 나라들의 경우는 $10^{4}$이상의 수준으로 유사한 수준을 나타내었다. 대장균군의 경우 미국산과 한국산, 중국산이 다소 높은 경향을 보였다.다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권10호
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• pp.1369-1378
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• 2006

This is the first study in which genetic variability and relationships of a large number of Japanese chicken breeds were revealed along with those of several foreign breeds by using microsatellite DNA polymorphisms. Twenty-eight breeds (34 populations) of native Japanese chickens and seven foreign breeds or varieties were analyzed. The mean number of alleles per locus, the proportion of the polymorphic loci, and the expected average heterozygosity ranged from 1.75 to 4.70, from 0.55 to 1.00, and from 0.21 to 0.67, respectively. Microsatellite alleles being unique to a particular population were detected in some populations. The $D_A$ genetic distance between populations was obtained from allele frequency for every pair of the populations to construct a neighbor-joining tree. According to the phylogenetic tree, excluding a few exceptions, native Japanese chicken breeds and foreign breeds were clearly separated from each other. Furthermore, the tree topology divided native Japanese chickens into four main classes, which was almost in accordance with the classification based on body morphology; that is, (1) Cochin type, (2) Malay type, (3) layer type, and (4) intermediate type between Malay and layer types. This is the first finding for native Japanese chickens.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.755-761
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• 2005

Blood samples were collected from eight native Japanese breeds of chickens (Miyadi-dori, Ohiki, Onaga-dori, Shoukoku, Tosa-Jidori, Tosa-Kukin, Toutenkou and Uzurao) and two foreign breeds of chickens (White Leghorn and Rhode Island Red) to examine the genetic variability and relationships among the breeds by using a microsatellite DNA technique. Except for the Shoukoku breed, the other Japanese chicken breeds all originate from Kochi Prefecture. Ohiki, Onaga-dori, Tosa-Jidori, Toutenkou and Uzurao are fancy fowl, and Miyadi-dori and Tosa-Kukin are utility fowl. Among the fancy fowl, Ohiki, Onaga-dori, and Toutenkou males have thick and long feathers in the saddle and tail. Genetic variabilities of the 20 microsatellites examined, varied depending on the breed: the mean number of alleles per locus ranged from 2.05 (Miyadi-dori) to 3.90 (Rhode Island Red); proportion of polymorphic loci ranged from 0.75 (Miyadi-dori) to 1.00 (Rhode Island Red, Shoukoku and Uzurao); and mean expected heterozygosity ranged from 0.330 (Miyadi-dori) to 0.607 (Rhode Island Red). Unique microsatellite alleles were detected in each breed. Using the neighbour-joining method, phylogenetic trees were constructed based on the genetic distances of D$_{A}$ and D$_{ST}$. Among the breeds originating from Kochi Prefecture, fancy and utility breeds belonged to different clusters. Among the fancy breeds, those having thick and long feathers in the tail and saddle showed a close genetic relationship to the Shoukoku breed, which also has thick and long feathers in the tail and saddle.

• 한국가금학회지
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• 제26권2호
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• pp.81-84
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• 1999

Chicken has been one of the most useful animals for life. They have been not only one of the most economical and adundant sources of valuable nutrients, but also the very good experimental animal to develop modern sciences. In Japan, eggs laid by the chickens have long been good sources of cash income for farmers and in many cases even for Samurais. Although nearly 50 millions of chickens were raised and they were of considerable importance in Japanese agricultre before the World War II, the industrialization or specializatin started after the War, Substantiallysince the 1960s. Among other spcies of poultry then chickens, ducks and Japanese quails are of some importane in Japan. Duck meat is often used in various cuisines at higher class restaurants and Japanese quail eggs are widely used. Hower those those species of commpared with chickens.

• Asian-Australasian Journal of Animal Sciences
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• 제21권12호
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• pp.1766-1772
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• 2008

Natto is a Japanese traditional soybean product fermented by Bacillus natto. The effect of natto supplement on the production and qualities of chicken meat was studied since the effective use of various waste foods is required in Japan. Dried natto prepared by heating at $60^{\circ}C$ was added to a basic diet at an amount of below 2%. The supplementation of dried natto did not influence the weights of the carcass, breast and thigh meat, fillet or abdominal fat. Growth of the thighbone such as the length, thickness of cortex bone, and Ca/P ratio in bone ash were not altered by the addition of natto. However, the pH of male meat decreased following the supplementation of dried natto from days 28 to 80. The water-soluble protein content in male thigh meat increased in the group fed 2% natto from days 28 to 80. Free peptides increased in male thigh meat by feeding 2% natto from days 0 to 80. The supplementation of natto increased free glutamic acid in thigh meat regardless of sex. Moreover, the supplementation of natto specifically decreased meat cholesterol in female chickens though the effect was not shown in male chickens.

• Asian-Australasian Journal of Animal Sciences
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• 제24권10호
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• pp.1329-1334
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• 2011

The Hinai-dori is a breed of chicken native to Akita Prefecture, Japan. An $F_2$ resource population produced by crossing low- and high-growth lines of the Hinai-dori breed was analyzed to detect quantitative trait loci (QTL) for growth traits. Highly significant QTLs for body weight at 10 and 14 weeks of age and average daily gain between 4 and 10 weeks and between 10 and 14 weeks of age were accordingly mapped in a common region between ADL0198 and ABR0287 on chromosome 1 and between MCW0240 and ABR0622 on chromosome 4, respectively. A significant QTL for body weight at 4 weeks of age and a significant QTL for average daily gain between 0 and 4 weeks of age were mapped for the first time to the same region flanking ABR0204 and ABR0284 on chromosome 1. These QTLs are good candidates for application in the development of marker-assisted selection strategies for increasing growth efficiencies in the Hinai-dori breed and native breeds of chickens in Asia.

• 한국동물위생학회지
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• 제26권1호
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• pp.11-18
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• 2003

A total of 1,024 sera were collected from cattle(227), pigs(465), chickens(257) and dogs(75) raised or slaughtered in Daejeon metropolitan city from April to September 2002. Japanses encephalitis virus(JEV) antibodies in sera were detected by the haemagglutination inhibition test. The prevalence rates of JEV antibodies were 99.1 %, 54.0 %, 63.0 % and 98.7 % in cattle, pigs, chickens and dogs, respectively. In case of cattle and dogs, the monthly antibody-positive rates were as high as 85.7∼100.0 % and there were no differences among six months. In case of pigs, the monthly antibody-positive rate showed the lowest in April(6.4 %) and the highest in July(100.0 %) and it remained above 50 % during the summer-time. In case of chickens, the monthly antibody-positive rate was 100.0 % in July & August, 80.5 % in June, 40.0 % in May, 7.5 % in September and 5.0 % in April in order and there were distinct differences in seasons.

• 농업과학연구
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• 제35권2호
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• pp.193-198
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• 2008

본 실험에서는 감귤, 매실, 당근의 껍데기로 감귤박, 매실박, 당근박을 먹여 산란계의 난황에 들어있는 Vitamin E와 K 함량을 높이고자 했다. Vitamin E 추출방법에는 검화방법인 ${\alpha}$, ${\alpha}$ - dipyridyl에 의한 비색정량법을 사용하였으며 전처리 과정 중 석유 ether를 가하여 층을 분리하는 과정과 증류수를 가하여 층을 분리하는 과정에 있어서 신중을 가하였고 신속한 시료 전처리 과정이 요구되었다. Vitamin K 추출에는 Diethyl dithiocarbamate에 의한 방법이 사용되었다. 실험결과 일반난황에서의 Vitamin E 함량보다 실험난황에서의 Vitamin E 함량이 2배 이상 높아졌음을 알 수 있었던 반면 Vitamin K의 함량은 소량 증가하여 큰 변화가 없었다. 위의 결과를 통해서 난황에 이전된 Vitamin E의 종류가 무엇이며 어떠한 형태의 Vitamin E가 이전이 많이 되었는지를 확인하는 실험이 필요할 것이다. 이러한 추가적인 실험이 이루어졌을 때 상업적으로 이용한 가치가 있는 가를 고려하여야 할 것이다.