• Mycobiology
• /
• 제35권3호
• /
• pp.159-161
• /
• 2007

Pine tree death caused by pine wood nematode(PWN) involves phoretic relationships between PWN and its vector Japanese pine sawyer beetle(JPS). In an effort to understand the diversity of fungi involved in PWN life cycle, a total of 176 fungal isolates were collected from PWNs, adults and larvae of JPS, PWN-diseased Japanese black pine that was cut down in 2005 at Jinju, Korea. Based on microscopic observation and colony morphology, and sequence analysis of the ITS rDNA, the fungal isolates were identified at the level of genus. Three genera including Mucor, Ophiostoma, and Penicillium were identified from PWN. Two genera of Ophiostoma and Penicillium were discovered from JPS larvae. Frpm JPS adult beetles, nine genera of Aspergillus, Gibberalla, Hypocrea, Irpex, Leptosphaeria, Ophiostoma, Penicillium, and Plectosphaerella and unknown basidio-mycetes were found. Ten genera from PWN-infected weed were confirmed as Bionectria, Botrytis, Camarops, Fusarium, Hypocrea, Nectria, Mucor, Ophiostoma, Penicillium, and Trichoderma. Penicillium and Ophiostoma were commonly distributed on PWN and its vector and host. This is first report of the fungi associated with PWN and its vector and host in Korea.

• Journal of Forest and Environmental Science
• /
• 제30권4호
• /
• pp.383-392
• /
• 2014

In this study, we investigated the Fukiage sand dunes of southern Kyushu, Japan. We surveyed the status of recovery of coastal Japanese black pine forests damaged by pine wilt disease and their disaster prevention capability. We placed two transects: Transect 1, in an area that was severely damaged (80-90% damage rate) by pine wilt disease, and Transect 2, in an area that was mostly undamaged (<10% damage rate). Then, we installed survey lines, carried out vegetation surveys, and measured the depth and pH of humus soil. The survey lines were placed perpendicular to the coastline from the top of the fore-dune to the inland area, and divided into five 50 m sections. Before the point 100 m inland from the top of the fore-dune, the number of invasive hardwoods and of Japanese black pines were small because of the poor growth environment in both transects. Past the 100 m point, the species and number of Japanese black pines and broad-leaved trees increased further inland because the growth environment improved. In addition, the recovery metrics of tree height, diameter at breast height, age, and number in Transect 1 were much lower than those in Transect 2, and the basal area of broad-leaved trees and the depth of humus soil in Transect 1 were lower than in Transect 2, and the soil pH of humus soil in Transect 1 was higher than that of Transect 2. The shape ratio of the Japanese black pine forests indicated that they were insufficient for disaster prevention. Therefore, in order to fully promote the disaster prevention capability of coastal Japanese black pine forests, we should not only focus on prevention of pine wilt disease but also undertake continuous control efforts taking into consideration the sound growth environment such as appropriate density and soil management and removal of invasive broad-leaved trees.

• Journal of Forest and Environmental Science
• /
• 제33권2호
• /
• pp.105-112
• /
• 2017

The distribution and form of pine wood nematode (PWN) were investigated in Jinju-si and Sacheon-si areas, where infested with pine wilt disease (PWD). The average PWN population per g of the tubulation part in the wilted Japanese balck pine were 381.2 and 341 in Jinju-si and Sacheon-si, respectively. The PWN population per g of affected Japanese black pine with wilt rate below 60% were 556 and 518 in Jinju-si and Sacheon-si, respectively. The ratios of PWN and other plant-parasitic nematode in the wilted Japanse black pines were 48% vs. 52% in Jinju-si and 53% vs. 47% in Sacheon-si after exposure to PWD for 1 year. The survivorship of pine wood nematode in the sampled trees after 1 year was 0-10% in the Jinju-si area and 5-20% detected in the Sacheon-si area. In the forest areas affected by PWN, the highest survival rates were 37.2% and 39.8% at 25 cm diameter at breast height (DBH) in Jinju-si and Sacehon-si, respectively, while the highest wilt rates were 30.5% and 28.3% at 30 cm DBH in Jinju-si and Sacehon-si, respectively.

• 한국응용곤충학회지
• /
• 제51권1호
• /
• pp.59-65
• /
• 2012

솔껍질깍지벌레는 우리나라 곰솔림의 주요한 해충의 하나이다. 본 연구는 우리나라에서 솔껍질깍지벌레의 분포를 알아보기 위하여 7개도 3개 광역시, 91개 시군구, 686 읍면동의 해송림에서 2010년 밀도를 조사하였다. 5개도 2개 광역시에서 솔껍질깍지벌레가 서식하고 있었는데 시군구별로는 조사대상의 64.8%인 59개 시군구에서 서식이 확인되었다. 솔껍질깍지벌레의 분포는 남해안 전역과 동해안의 포항, 서해안의 충남 보령지역까지 분포하였다. 충북과 대전광역시 및 제주도에서는 솔껍질깍지벌레의 분포가 확인되지 않았다. 부산지역은 16곳의 모든 구에서 솔껍질깍지벌레가 분포하였고, 시군 단위에서는 전남 해남군의 솔껍질깍지벌레 평균밀도가 1.713마리/0.785 $cm^2$로 가장 높았으며 읍면동 단위에서는 해남군 송지면이 6.36마리/0.785 $cm^2$로 가장 높았다. 곰솔 가지 내의 솔껍질깍지벌레 밀도와 솔껍질깍지벌레가 서식하고 있는 빈도와는 높은 상관관계가 있었다(상관계수=0.89).

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2014년도 춘계학술대회 및 임시총회
• /
• pp.41-41
• /
• 2014

Pinewood nematode (PWN, Bursaphelenchus xylophilus) is a serious pathogenic worm that quickly dry pine trees to death. Recently, PWN has been devastating huge amounts of conifer trees in Korea. As a first step to explore the association and ecological roles of fungi in PWN life cycle in Korea, in this study we first isolated and indentified fungi from PWN-infested Korean pine and Japanese black pine wood sampled in Jinju, Sacheon, Pocheon, Chuncheon, Gwangju, and Hoengseong in Korea. A total of 144 fungal isolates were obtained from Japanese black pine wood and 264 fungal isolates from Korean pine wood. Their morphology and nucleotide sequences of the ITS rDNA and ♌-tubulin gene were examined for species identification. Ophiostoma ips, Botrytis anthophila, Penicillium sp., Hypocrea lixii, Trichoderma atroviride, O. galeiforme, Fusarium proliferatum were identified from Japanese black pine wood. Leptographium koreanum, L. pini-densiflorae, Ophiostoma ips, Penicillium raistrick, Trichoderma sp. were isolated from Korean pine wood. O. ips and L. koreanum were the major species on the two different PWN-infected pine tree. The cultivation of PWN on fungal mat of the identified species did some enhance PWN reproduction. The ambrosia beetle, Platypus koryoensis, is a serious pest of oak trees in Korea. In this study we investigated filamentous fungi present in the body of the beetle. Fourteen genera of filamentous fungi belonging to Ascomycota and Basidiomycota were isolated. All the obtained genera were isolated in the mitosporic state. The identified fungi were classified in 11 distinct orders including the Ascomycota (Eurotiales, Hypocreales, Microascales, Ophiostomatales, Pleosporales, and Sordiales) and Basidiomycota (Agaricales, Corticiales, Polyporales, and Russulales Xylariales). Within Ascomycota, 13 species were found. Meanwhile five species were found within Basidiomycota. The results showed the presence of diverse fungi in P. koryoensis. Among the isolated fungi, some were able to produce wood degrading enzymes. Further fungal isolation was performed with P. koryoensis infested Quercus mongolica trees sampled at Kumdan mountain in Hanam-Si, Gyeonggi province from June of 2009 to June of 2010. Penicillin spp. and Trichoderma spp. were the major species of mold fungi group. Pichia guilliermondii was the major species of mold yeast group. Raffaelea quercus-mongolicae was also isolated, but its isolation frequency was not high. Other species identified were Ambrosiella xylebori, Fusarium solani, Cryphonectria nitschke, Chaetomium globosum, and Gliocladium viride, Candida kashinagacola, C. maritima, C. vanderkliftii, Saccharomycopsis crataegensis.

• 한국산림과학회지
• /
• 제87권4호
• /
• pp.543-548
• /
• 1998

소나무와 해송으로부터 엽록체상의 두 유전자 psbD와 rbcL를 PCR에 의해 증폭한 후 제한효소 HaeIII를 사용해서 절단했다. 두 개의 종 특이적 엽록체 DNA 단편이 확인되었고, 이 두 개의 표지자를 이용하여 소나무(충북3호)와 해송(남난37호)의 인공교잡 가계로부터 엽록체 DNA의 부계 유전양식이 확인되었다. 인공교잡 가계에 있어서 엽록체 DNA의 부계 유전양식을 근거로 해송으로부터 소나무로의 이입교잡에 의해 생겨났다는 가설(현신규 등, 1967)이 지배적인 금강송 115개체로부터 이입교잡에 의해 유입되어진 흔적을 구명하기 위하여 해송 특이 엽록체 DNA의 존재 여부를 검색하였다. 분석에 사용된 금강송 전 개체에서 소나무에서 관찰된 엽록체 DNA(psbD와 rbeL)의 절편 분획 양상과 동일한 절편 분획 양상이 확인되었다. 본 실험의 결과로부터는, 금강송에는 해송으로부터 유입된 엽록체 게놈의 흔적을 찾아 볼 수 없었으며, 따라서 금강송을 소나무(♀)약 해송(♂)의 이입교잡종이이라고 간주할만한 확고한 증거를 제시할 수 없었다.

• 한국가구학회지
• /
• 제19권4호
• /
• pp.229-234
• /
• 2008

In a previous study, it was revealed that three major softwoods, Japanese pine, Korean pine and Japanese larch, heat-treated at $220^{\circ}C$, could produce high quality dark-colored boards. It is known that heat treatment decreases the hygroscopicity of wood. The hygroscopicity of major domestic softwoods and hardwoods heat-treated at $220^{\circ}C$ was investigated by a saturated salt solution method and compared with that of black and white charcoals. Equilibrium moisture contents of wood decreased with the increase of heat treatment time. Isotherm shapes of wood species were different from those of charcoals. Heat treatment decreases the equilibrium moisture contents of black locust more than those of Korean pine and Japanese larch. It was found that surface hardness of wood is improved by heat treatment to a certain extent, but a longer heat treatment causes thermal degradation, resulting in the decrease of the surface hardness.

• Journal of the Korean Wood Science and Technology
• /
• 제26권4호
• /
• pp.34-42
• /
• 1998

Three hardwood (ash, alder and black locust) and three softwood (Japanese red pine, radiata pine and Western hemlock) specimens were dried in microwave(MlW) oven and their drying rates were obtained. Their specific permeabilities were also measured by using a modified liquid permeability measuring device. The correlation between the M/W drying rates and permeabilities of six species were statistically analyzed. It was revealed that within a species there is a logarithmic relationship between the M/W drying rates and average moisture contents and that among species there is a linear relationship between the M/W maximum drying rates and the average specific permeabilities. A exception was Western hemlock, which was of low permeability and of high drying rate. A Me-time equation, which showed a good agreement with the actual data, was derived. Thus using this equation the moisture contents of wood and the drying end points of M/W drying could be predicted. Infrared images of the thermal distribution in wood were illustrated.

• 한국가구학회지
• /
• 제16권2호
• /
• pp.59-65
• /
• 2005

As physical condition of students improves, there is a need to develop human body-friendly desk and chair for students. In this study, desks and chairs were manufactured with curved veneer lamination under high frequency heating and pressing, using ten wood species such as Japanese red pine, Korean pine, pitch pine, Japanese larch, yellow poplar, black locust, oak, radiata pine, beech, and birch. The performance of these products were evaluated. The results obtained were summarized as follows; With high frequency heating, the turned lamination of veneers with full size sheet ($3{\times}6\;feet$) prepared by rotary lathe peeling was successfully applied for making the members of desk top, leg frames of desk and chair. Bending strengths of desk tops were relatively greater for yellow poplar, black locust and red pine, which were similar to those of beech and birch. Bending strengths of desk legs were classified into greater species group (red pine, yellow poplar, larch) and lower species group (radiata pine, Korean pine, pitch pine). Compressive strengths of chair legs in parallel direction to the lamination were greater in black locust and larch. On the other hand, differences between outer and inner gap at the top and drawer bottom of desk top were rather larger for the laminations of birch and beech, and less for those of yellow poplar and pitch pine, showing greater stability of open drawer space. In results, yellow poplar, larch, pitch pine and red pine showed good appearance and strength properties at the curved veneer lamination. Accordingly, it was believed that these domestic woods were able to substitute for birch which was being imported for the use of veneer-laminates type furniture.

• 한국응용곤충학회지
• /
• 제57권3호
• /
• pp.143-149
• /
• 2018

솔껍질깍지벌레(Matsucoccus thunbergianae)는 우리나라 곰솔(Pinus thunbergiane)림의 주요 해충중의 하나이다. 솔껍질깍지벌레는 단목(丹木)마다 밀도에 차이가 있고, 수관 내 가지별로도 밀도차이가 있어 예찰이 어렵다. 따라서 본 연구는 황색 끈끈이트랩을 이용하여 솔껍질깍지벌레의 분포나 발생을 예찰할 수 있는 방법을 제시하기 위하여 수행되었다. 곰솔림에서 끈끈이 트랩에 유인된 솔껍질깍지벌레 수컷 성충의 밀도와 가지 내 약충의 밀도는 높은 상관관계를 보였다. 끈끈이트랩에 유인된 솔껍질깍지벌레 수컷성충의 밀도는 곰솔의 상층부가 하층부보다 높았으나 통계적 차이는 없었다. 곰솔 가지의 단위면적당 솔껍질깍지벌레 밀도는 흉고직경이 클수록 높아지는 경향을 보였으며 1~2.5 cm 사이의 굵기내에서는 통계적 차이가 없었다. 곰솔 가지의 방향이 지면을 향하는 쪽이 반대 방향을 향하는 쪽에 비하여 솔껍질깍지벌레의 밀도가 높았다. 솔껍질깍지벌레의 예찰에 끈끈이 트랩의 사용을 제안하며, 가지에서의 밀도는 2~2.5 cm 굵기의 가지를 선택하여 지면쪽의 밀도 조사를 추천한다.