• Title/Summary/Keyword: Janibacter sp.

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Identification and Cultural Characterization of Lipase Production Bacteria Isolated from Pond Effluent Sedimentary Layer (양식장 배출수 퇴적층에서 분리된 리파아제 생산 박테리아의 동정 및 배양학적 특성)

  • Kim, Man-Chul;Jang, Tae-Won;Harikrishnan, Ramasamy;Jang, Ik-Soo;Yeo, In-Kyu;Jeong, Joon-Bum;Heo, Moon-Soo
    • Korean Journal of Microbiology
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    • v.45 no.1
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    • pp.58-62
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    • 2009
  • From the course of screening of useful enzyme producing microorganism from marine sedimentary layer, we isolated 2 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram-positive bacteria grouped on Janibacter sp. An excellent lipase producing strain, Janibacter sp. LI-68 and J. sp. LI-80 identified by 16S rDNA analysis and biochemical methods (BIOLOG), was further studied its lipase producing characteristics. The optimum initial pH, temperature and the optimum cultral time for the enzyme production on MA medium were 8, $30{\sim}40^{\circ}C$ and 96 h, respectively.