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Identification and Cultural Characterization of Lipase Production Bacteria Isolated from Pond Effluent Sedimentary Layer  

Kim, Man-Chul (Faculty of Marine Science, Jeju National University)
Jang, Tae-Won (Faculty of Marine Science, Jeju National University)
Harikrishnan, Ramasamy (Faculty of Marine Science, Jeju National University)
Jang, Ik-Soo (Faculty of Marine Science, Jeju National University)
Yeo, In-Kyu (Faculty of Marine Science, Jeju National University)
Jeong, Joon-Bum (Faculty of Marine Science, Jeju National University)
Heo, Moon-Soo (Faculty of Marine Science, Jeju National University)
Publication Information
Korean Journal of Microbiology / v.45, no.1, 2009 , pp. 58-62 More about this Journal
Abstract
From the course of screening of useful enzyme producing microorganism from marine sedimentary layer, we isolated 2 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram-positive bacteria grouped on Janibacter sp. An excellent lipase producing strain, Janibacter sp. LI-68 and J. sp. LI-80 identified by 16S rDNA analysis and biochemical methods (BIOLOG), was further studied its lipase producing characteristics. The optimum initial pH, temperature and the optimum cultral time for the enzyme production on MA medium were 8, $30{\sim}40^{\circ}C$ and 96 h, respectively.
Keywords
16S rDNA; Janibacter sp.; lipase; marine sedimentary layer;
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