Browse > Article

Identification and Cultural Characterization of Lipase Production Bacteria Isolated from Pond Effluent Sedimentary Layer  

Kim, Man-Chul (Faculty of Marine Science, Jeju National University)
Jang, Tae-Won (Faculty of Marine Science, Jeju National University)
Harikrishnan, Ramasamy (Faculty of Marine Science, Jeju National University)
Jang, Ik-Soo (Faculty of Marine Science, Jeju National University)
Yeo, In-Kyu (Faculty of Marine Science, Jeju National University)
Jeong, Joon-Bum (Faculty of Marine Science, Jeju National University)
Heo, Moon-Soo (Faculty of Marine Science, Jeju National University)
Publication Information
Korean Journal of Microbiology / v.45, no.1, 2009 , pp. 58-62 More about this Journal
Abstract
From the course of screening of useful enzyme producing microorganism from marine sedimentary layer, we isolated 2 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram-positive bacteria grouped on Janibacter sp. An excellent lipase producing strain, Janibacter sp. LI-68 and J. sp. LI-80 identified by 16S rDNA analysis and biochemical methods (BIOLOG), was further studied its lipase producing characteristics. The optimum initial pH, temperature and the optimum cultral time for the enzyme production on MA medium were 8, $30{\sim}40^{\circ}C$ and 96 h, respectively.
Keywords
16S rDNA; Janibacter sp.; lipase; marine sedimentary layer;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Bjorkling, F., S.E. Godtfredsen, and O. Kirt. 1991. The future impact of industrial lipases. Trends Biotechnol. 9, 360-363   DOI   ScienceOn
2 Desnuelle, P. 1972. The lipases, pp. 575-616. In P.D. Boyer (ed.) Academic Press, New York and London
3 Lee, S.A., J.H. Lee, S.J. Kim, and H.K. Kim. 2005. Hydrolysis of triglyceride with cold-adapted lipase of Psychrobacter sp. S3 isolated from intertidal flat. Kor. J. Microbiol. Biotechnol. 33, 29-34   과학기술학회마을
4 Liu, W.A., T. Beppu, and K. Arima. 1973. Substrate specificity and mode of the lipase of thermophilic fungus Humicola lanuginosa S-38. Agri. Biol. Chem. 37, 1349-1355   DOI
5 Malcata, F.X. 1996. Engineering of/with lipases: Scope and strategies, pp. 1-16, Kluwer Academic Publishers, Netherlands
6 Park, D.S., H.W. Oh, K.S. Bae, H.M. Kim, S.Y. Heo, N.J. Kim, K.Y. Seol, and H.Y. Park. 2007. Screening of bacteria producing lipase from insect gut: Isolation and characterization of a strain, Burkholderia sp. HY-10 producing lipase. Kor. J. Appl. Entomol. 46, 131-139   과학기술학회마을   DOI
7 Park, M.H., H.J. Ryu, and K.K. Oh. 2004. Isolation of lipase producing yeast and optimization of cultivation condition. Kor. J. Biotechnol. Bioeng. 19, 148-153   과학기술학회마을   ScienceOn
8 Jaeger, K.K., H.J. Choi, M.H. Kim, C.B. Sohn, and T.K. Oh. 2002. Expression and characterization of Ca$^2+$-dependent lipase from Bacillus pumilus B26. Biochim. Biophys. Acta. 1583, 205-212   DOI   PUBMED   ScienceOn
9 Herbert, R.A. 1992. The perspective on the biotechnological potential of extremophiles. Trends Biotechnol. 10, 395-402   DOI   PUBMED   ScienceOn
10 Jaeger, K.E., S.W. Dijkstra, and M.T. Reetz. 1999. Bacterial biocalysis: Molecular biology, three-dimensional structures, and biotechnological applications of lipase. Annu. Rev. Microbiol. 53, 315-351   DOI   ScienceOn
11 Feller, G., E. Narinx, J.L. Arpigny, M. Aittaleb, E. Baise, S. Genicot, and C. Gerday. 1996. Enzymes from psychrophilic organisms. FEMS Microbiol. Rev. 18, 189-202   DOI   ScienceOn
12 Iwai, M., S. Okumura, and Y. Tsujsaka. 1975. The comparison of the properties of two lipases from Penicillin cyclopium westring. Agri. Biol. Chem. 39, 1063-1070   DOI
13 Taipa, M.A., M.R. Aires-Barros, and J.M.S. Cabral. 1992. Purification of lipase. J. Bacteriol. 26, 111-142   DOI   ScienceOn
14 Ren, T.J., F. Frank, and G.L. Christen. 1988. Characterization of lipase of Pseudomonas fluorescens 27; Based on fatty acid profiles. J. Dairy Sci. 71, 1432-1438   DOI   ScienceOn
15 Ryu, H.S., H.K. Kim, W.C. Choi, M.H. Kim, S.Y. Park, N.S. Han, T.K. Oh, and J.K. Lee. 2006. New cold-adapted lipase from Photobacterium lipolyticum sp. nov. that is closely related to filamentous fungal lipases. Appl. Microbiol. Biotechnol. 70, 321-326   DOI   ScienceOn
16 Lee, J.M., R.S. Kim, B.O. Kim, Y.D. Park, and I.N. Jin. 1993. Isolation of a Pseudomonas aerusinosa strain producing an extracellular alkaline lipase catabolitely regulated by glucose, and purification of the Lipase. Kor. J. Appl. Microbiol. Biotechnol. 21, 161-168   과학기술학회마을
17 Reetz, M.T. 2002. Lipases as practical biocatalysts. Curr. Opin. Chem. Biol. 6, 145-150   DOI   PUBMED   ScienceOn
18 Noble, L.D. and J.A. Gow. 1998. The effect of suspending solution supplemented with marine cations on the oxidation of Biolog GNMicroPlate$^TM$ substrates by Vibrionaceae bacteria. Can. J. Microbiol. 44, 251-258   DOI   ScienceOn
19 Otero, C., M.A. Berrendero, F. Cardenas, E. Alvarez, and S.W. Elson. 2005. General characterization of noncommercial microbial lipase in hydrolytic and synthetic reactions. Appl. Biochem. Biotechnol. 120, 209-223   DOI   ScienceOn
20 Sztajer, H. and E. Zboinsk. 1988. Microbial lipase in biotechnology. Acta Biotechnol. 2, 169-175   DOI
21 Kim, J.W., S.Y. Shim, and S.S. Yoon. 1997. Isolation and purification of a lipase from Pseudomonas sp. YJ103 isolated from raw milk. Kor. J. Dairy Sci. 19, 17-24
22 Kumar, S., K. Tamura, and M. Nei. 2004. Mega 3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinformatics 5, 150-163   DOI   ScienceOn