• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.15 no.1
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• pp.127-139
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• 2002

This study was carried out to prove the therapeutic effects of BNJB on the catract. The objects of this study were CXSD mice that spontaneously eye rupture occurred from three weeks after birth and eventually generate cataract within 12 weeks. We applied eyewash made from BNJB to eyes of CXSD mice twice in a day till all the eyes of the negative control showed up the symptoms of a cataract and recorded the increasing patterns of cataractous eyes. To explained the therapeutic effects of the BNJB, We carried out the ex vivo experiment which cultivating eyeballs were offered oxidative stress condition by $0.03{\%}$ $H_2O_2$ during three days. Total co-enzyme was extracted from the cultured eyeballs and used to measure activities of catalase, superoxide dismutase, glutathion S-transferase and content of GSH. The results were obtained as follows: 1. When we treated the catalin to CXSD mouse as a positive control, it represented the delaying effect for cataract generation for 2-3 days compare with negative control. But it seems that it doesn't appropriate as a therapeutic, or delaying agent. 2. In the experimental BNJB group, Cataract formation rate was dramatically reduced by BNJB. This rate was much lower than positive group and means our new formulation for the therapy of cataract has a good potential. 3. In the analysis of individual medicines of BNJB, Mok-Jeok-Cho, Hwang-Lyun and Ha-Go-Cho didn't have a major effect of BNJB. 4. The cataract formation rate was repressed by Bing-Pyun and Jin- Joo-Boon about $40{\%}$ and $50{\%}$, respectively. We can presume that the anti-cataract effect of BNJB was caused by these two medicines. 5. When we surveyed the anti-oxidant activities of BNJB, enzyme activities of GSH, SOD, and Catalase increased about $10{\%},\30{\%}$, and 2.5 folds, respectively.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.3
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• pp.289-295
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• 2014

In order to discover ingredients for wrinkle-care cosmetics, we prepared 70% ethanol extract from Gynostemma pentaphyllum and examined its activity on collagen synthesis using fibroblast HDFn cells. The G. pentaphyllum extract induced the production of type I procollagen in a dose-dependent manner without showing cell toxicity. The active constituents were isolated from the extract by solvent fractionation and chromatographic purification procedures. NMR data and literature studies led to determine the two isolated compounds as the flavonoid glycosides such as ombuine 3-O-rutinoside (1) and quercetin 3-O-rutinoside (2). The activity screening tests showed that the isolates 1 and 2 induced the production of type I procollagen in a dose-dependent manner. These results suggested that G. pentaphyllum extract containing the flavonoids 1 and 2 could be useful as an active ingredient for wrinkle-care cosmetics.

• Archives of Pharmacal Research
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• v.27 no.12
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• pp.1220-1225
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• 2004

The overproduction of nitric oxide (NO) by inducible nitric oxide synthase (iNOS) is known to be responsible for vasodilation and hypotension observed in septic shock and inflammation. Inhibitors of iNOS, thus, may be useful candidates for the treatment of inflammatory diseases accompanied by overproduction of NO. In the course of screening oriental anti-inflammatory herbs for the inhibitory activity of NO synthesis, a crude methanolic extract of Curcuma zedoaria exhibited significant activity. The activity-guided fractionation and repetitive chromatographic procedures with the EtOAc soluble fraction allowed us to isolate three active compounds. They were identified as 1,7-bis (4-hydroxyphenyl)-1,4,6-heptatrien-3-one (1), procurcumenol (2) and epiprocurcumenol (3) by spectral data analyses. Their concentrations for the 50% inhibition of NO production $(IC_{50})$ in lipopolysaccharide (LPS)-activated macrophages were 8, 75, 77 ${\mu}M$, respectively. Compound 1 showed the most potent inhibitory activity for NO production in LPS-activated macrophages, while the epimeric isomers, compound 2 and 3 showed weak and similar potency. Inhibition of NO synthesis by compound 1 was very weak when activated macrophages were treated with 1 after iNOS induction. In the immunoblot analysis, compound 1 suppressed the expression of iNOS in a dose-dependent manner. In summary, 1,7-bis (4-hydroxyphenyl)-1,4,6-heptatrien-3-one from Curcuma zedoaria inhibited NO production in LPS-activated macrophages through suppression of iNOS expression. These results imply that the traditional use of C. zedoaria rhizome as anti-inflammatory drug may be explained at least in part, by inhibition of NO production.

• YAKHAK HOEJI
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• v.53 no.6
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• pp.334-341
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• 2009

Aging society and aged society are determined by the component ratio of the elderly to the whole population; aging society is defined as a society whose ratio of the elderly population is less than 7%, and it is called aged society when the ratio is over 14%. Korea's aging process is so fast and unprecedented over the world according to many statistical data and investigations. The purpose of this study was to provide guidance and basic data to educate the elderly for the proper use of medication and health functional food. This study was carried out on 80 subjects of age 65 or older in Cheong Jusi in Choogncheongbuk-do. They answered questionnaire that was prepared for this study. The data were collected from August 4, 2008 to August 18, 2008. The collected data were analyzed by using the SPSS 12.0OK (for window) program and Microsoft EXCEL. The following is a summary of the results of this study. First, in the aspect of purchasing, the majority of respondents bought OTC drugs and health functional food for health and illness prevention. Purchasing places of drugs were diverse. Second, in the aspect of taking drugs, the older answerers were, the higher the number of drugs being taken was. Third, in the aspect of proper storage of drugs, most respondents have no specific spaces. Finally, regarding recognition of drug abuse and disposal, most people have little awareness or have no idea.

• Journal of Veterinary Clinics
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• v.26 no.3
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• pp.276-278
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• 2009

A 1 year 4 months old, male Poodle was presented with a history of non weight bearing lameness and pain of the right forelimb by trauma. Orthopedic examination and radiographs were revealed medial shoulder luxation and scapular fracture. Biceps tendon was translocated to the lesser tubercle and secured to the humerus. Despite of it could be a potential cause of degenerative change of the joint, a favorable result was achieved for more than 3 years follow-up.

• Food Science of Animal Resources
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• v.32 no.2
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• pp.241-246
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• 2012

This report outlines the development of a rapid, simple, and sensitive detection system for pathogenic bacteria using a capillary electrophoresis-based, single strand conformation polymorphism (CE-SSCP) combined with PCR. We demonstrate that this method, used with primers targeting the V4 region of the16S rRNA gene, is capable of the simultaneous detection of 10 microbes that could be associated with foodborne illness, caused by animal-derived foods: Salmonella enterica, Listeria monocytogenes, Escherichia coli O157:H7, Campylobacter jejuni, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Yersinia enterocolitica, Vibrio parahaemolyticus, and Enterobacter sakazakii. The traditional detection techniques are time-consuming and labor-intensive, due to the necessary task of separate cultivation of each target species. As such, the CE-SSCP-PCR method, that we have developed, has the potential to diagnose pathogens rapidly, unlike the traditional technique, in order to prevent foodborne illness in a much more efficient manner.

• International Journal of Oral Biology
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• v.36 no.3
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• pp.123-128
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• 2011

The notion that dental amalgam is a potential source of mercury exposure remains a controversial issue. However, there are few epidemiological analyses that have addressed whether this occurs in children. We aimed in our current study to identify the relationship between dental amalgam filling surfaces and the blood mercury levels in a cohort of 711 South Korean children aged between 8-9 years. Oral examinations were conducted to detect the number of amalgam filling surfaces on the teeth of these individuals. Blood samples were also taken from these children to assess the levels of mercury accumulation in the body. The amalgam filling surfaces were classified into four groups based on their number: 0 (n = 368), 1-5 (n = 219), 6-10 (n = 89), and 11+ (n = 35). The blood mercury levels in the children with more than 10 amalgam surfaces was 0.47 ${\mu}g$/L higher on average than those with no amalgam surfaces after adjusting for the frequency of fish or seafood consumption, age, and gender (P < 0.05). We found from our data that a higher number of dental amalgam fillings correlated with a higher blood mercury level in Korean children. Further studies are needed to investigate whether these elevated mercury levels exert neurotoxic or nephrotoxic effects.

• Tuberculosis and Respiratory Diseases
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• v.43 no.3
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• pp.434-439
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• 1996

Although the incidence of pulmonary tuberculosis is declining, the number of extrapulmonary tuberculosis has remained constant. Tuberculous Lymphadenitis accounts for over 50% of total inflammatory lymphadenitis and the most common site is cervical lymph node. We report a case of single cervical tuberculous cold abscess associated with multiloculated and septated tuberculous pleurisy. Intracavitary urokinase instillation via percutaneous catheter is indicated in loculated and septated pleural effusion. And our result was satisfactory without complication.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2010.11a
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• pp.713-716
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• 2010

Slosh and vibration effects of fuel inside of fuel tank can be occurred due to the acceleration and flight speed during the rotorcraft flight. It can lead to the failure of internal fuel component and fuel tank skin can be damaged. This is directly related to human survival. Military specification (MIL-DTL-27422D) specifies that stability of aircraft fuel tank and internal component against slosh &vibration load shall be verified through the qualification test procedures. This report shows the establishment of slosh and vibration test facility and KUH fuel tank qualification test result.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.1
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• pp.31-39
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• 2017

The effects of application of gypsum and fresh cattle manure on the yield of forage crop were investigated in Hwaong reclaimed land in Korea for 3 years from 2011 to 2013. This study was conducted to develop the practical application method of livestock manure as a fertilization source and a soil physico-chemical ameliorator for the cultivation of forage crop $Sorghum{\times}Sudangrass$ hybrid in newly reclaimed tidal land soil. Treatments with six applications were established with three replications; chemical fertilizer (CF), gypsum (G) $20Mg\;ha^{-1}$, G+fresh cattle manure (FCM) 100%, G+FCM 200%, G+FCM 300% and FCM 100% which referred to the application rate equivalent to the recommended amount of phosphate fertilization by soil test. The combined treatments of G+FCM increased soil organic matter, $Av.P_2O_5$ and exchangeable $Ca^{2+}$ contents while decreased exchangeable $Na^+$ and $Mg^{2+}$. The soil bulk density, soil hardness and soil aggregate formation were improved by G+FCM treatments. The dry matter yields of $Sorghum{\times}Sudangrass$ hybrid were significantly increased in proportion to the application rate of FCM. The phosphorus use efficiency showed the highest in the application level of G+FCM 100%, which seemed to be the results of reduced nutrient use efficiency by nutrient immobilization, leaching etc. when applied excessive amount of fresh animal manure.