• 한국수산과학회지
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• 제30권3호
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• pp.388-392
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• 1997

해조류중 우리나라 전연안에 가장 흔히 분포하는 갈파래목 녹조류인 참홑파래와 구멍갈파래, 참갈파래, 잎파래, 실파래, 가시파래등을 대상으로 RAPD방법을 이용한 각 지역 개체간의 유전적 유사도를 조사하였다. Total DNA는 엽체로부터 LiCl를 사용한 DNA추출방법으로 분리하였으며, 추출된 DNA는 $3ng/{\mu}\ell$되게 희석한후 in vitro에서 arbitrary primer와 함께 45회 PCR amplification시켰다. 그 결과 34종류 primer들로부터 240bp에서 1.5kb의 다양한 크기로 증폭된 1227개의 전기영동상의 band를 볼 수 있었으며, Jaccard공식에 따라 그 유사도를 구하였다. 파래목의 유전적 유사도는 $7\%$에서 $36\%$범위의 유사도를 보였다. 그중 참흩파래가 다른 갈파래과들로부터 가장 낮은 유사도값을 보여주었으며, 또한 primer OPB-01 (CATCCCCCTG)을 사용하였을때 갈파래과에서 공통적인 630bp크기의 생성물을 생산하지않는 특징을 나타내었다.

• 한방재활의학과학회지
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• 제32권1호
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• pp.107-124
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• 2022

Objectives This study aimed to identify optimal combinations of acupoints used to treat chemotherapy-induced peripheral neuropathy (CIPN). Methods We searched four international databases (MEDLINE, EMBASE, the Allied and Complementary Medicine Databases [AMED], and China National Knowledge Infrastructure [CNKI]) and five Korean databases (DBpia, Research Information Sharing Service [RISS], Korean Studies Information Service System [KISS], Oriental Medicine Advanced Searching Integrated System [OASIS], and KoreaMed) to identify randomized controlled trials (RCTs) that used acupuncture to treat CIPN. Network analysis was performed on the acupoints used in more than three included articles. We constructed a network by calculating the Jaccard similarity coefficient between acupoints and applied minimum spanning tree. Then, modularity analysis, degree centrality (Cd), and betweenness centrality (Cb) were used to analyze properties of the acupoints. Results A total of 25 articles were included. 24 acupoints were extracted from 25 articles. The combinations of acupoints having the highest Jaccard similarity coefficient were {EX-UE9, EX-LE10} and {ST36, SP6}. In the modularity analysis, acupoints were classified to six modules. ST40, EX-UE11, and KI6 had the highest Cd value while ST40, GB34 had the highest Cb value. Conclusions This study found the systematic framework of acupoint combinations used in CIPN studies. This study is expected to provide new perspectives of CIPN treatment to therapists. A RCT is in progress of using the network of this study as a guideline. If significant results are derived from the RCT, it will be possible to lay the groundwork to consider acupuncture for CIPN treatment.

• Journal of Plant Biotechnology
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• 제43권3호
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• pp.367-375
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• 2016

국내에서 육성된 옥수수 90 품종 및 자식 계통에 대하여 microsatellite 마커를 활용하여 DNA 프로파일 데이터베이스를 구축한 다음 공시품종에 따른 유전적 유사도 분석 및 품종식별력 검정에 대한 연구를 수행하였다. 옥수수 90품종을 100개의 microsatellite 마커로 검정하고 대립유전자의 패턴이 우수하고 다형성 정도가 높은 13개를 선정하여 분석하였을 때 대립유전자의 수는 5 ~ 24개까지 다양하게 분포하였고 평균 대립유전자의 수는 13.69개로 높았다. PIC 값의 경우도 0.716 ~ 0.942 범위에 속하였고 평균값은 0.865로 아주 높았다. 옥수수 90품종 및 계통에 대하여 UPGMA 분석에 의한 계통도를 작성하였을 때, 옥수수의 품종 유형 및 품종 육성 계보에 따라 5개의 대그룹으로 나누어졌다. 본 연구에서 구축됨 옥수수 자식계통 및 품종별 microsatellite DNA 프로파일 데이터베이스는 신품종과 기 육성된 품종과 유전적 유사도 분석이 가능하기 때문에 품종보호출원시 대조품종 선정 및 품종진위성과 관련된 종자분쟁에 매우 유용하게 활용될 수 있을 것이다.

• Journal of Plant Biotechnology
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• 제46권1호
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• pp.1-8
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• 2019

The main goal of this study was to determine the genetic diversity among 36 grape cultivars grown in Palestine by using ISSR-polymerase chain reaction (PCR) fingerprints. Among the tested primers, 17 produced reasonable amplification products with high intensity and pattern stability. A total of 57 DNA fragments (loci) separated by electrophoresis on agarose gels were detected and they ranged in size, from 150 to 900 bp. Out of these fragments, 55 (88%) were polymorphic and 2 (3.5%) monomorphic. Our results also revealed an average of 3.1 loci per primer. A minimum of 1 and maximum of 10 DNA fragments were obtained (S-17, #820 and #841) and (S-31) primers, respectively. Therefore, the later primer (S-31) is considered to be the most powerful primer among the tested ones. The genetic distance matrix showed an average distance range of between 0.05 and 0.76. The maximum genetic distance value of 0.76 (24% similarity) was exhibited between the (Shami and Marawi.Hamadani.Adi) as well as (Bairuti and Marawi.Hamadani.Adi) genotypes. On the other hand, the lowest genetic distance of 0.05 (95% similarity) was exhibited between (Jandali.Tawel.Mofarad and Jandali. Kurawi.Mlzlz) along with (Shami.Aswad and Shami.mtartash. mlwn) genotypes. Furthermore, the UPGMA dendrogram generally clusters the grape cultivars into eight major clusters in addition to an isolated genotype. Based on these figures, the cultivars tested in this study could be characterized by large divergence at the DNA level. This is taking the assumption that our region has a very rich and varied clonal grape genetic structure.

• KNOM Review
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• 제22권3호
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• pp.20-24
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• 2019

블록체인은 분산 원장 기반 기술로 블록체인 네트워크에 참여하는 모든 노드들은 P2P 네트워크로 연결되어 있다. 블록체인 네트워크에서 트랜잭션이 생성되면, 트랜잭션은 블록체인 노드들에 의해 전파되고 유효성을 검증 받는다. 검증된 트랜잭션은 P2P 네트워크를 통해 각 노드와 연결되어있는 Peer들에게 전송되고, Peer들은 트랜잭션을 메모리 풀에 보관한다. P2P 네트워크 특성상 블록체인 노드가 전달하는 트랜잭션의 수와 종류는 각 노드마다 달라 모든 노드들이 동일한 메모리 풀을갖지 못하는 문제가 발생함에 따라 메모리 풀에 저장되어있는 트랜잭션들은 거래 수수료 조작, 이중 지불 문제, DDos 공격탐지 등의 문제를 해결하기 위해 연구가 필요하다. 본 논문에서는 거래 수수료 조작, 이중 지불 문제, DDos공격 탐지 등과 같은 문제를 해결하기 앞서 메모리 풀의 트랜잭션들을 분석한다. 따라서 본 연구는 블록체인기술을 기반으로 구현된 암호화폐 시스템인 비트코인과 이더리움의 각 노드 메모리 풀에 저장되어있는 트랜잭션들을 수집하고 얼마만큼의 공통된 트랜잭션들을 가지고 있는지 자카드 유사도를 이용하여 분석한다.

• Journal of Ecology and Environment
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• 제43권2호
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• pp.183-190
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• 2019

Background: In the Philippines, the practice of planting mangroves over seagrass has been a practice done to promote coastline protection from damages done by storms. Despite the added protection to the coastline, the addition of an artificial ecosystem gradually inflicts damage to the ecosystem already established. In this study, seagrass communities that had no history of mangrove planting were compared with those that had mangrove planting. The percent substrate cover of seagrass in the sampling areas was determined, and the macroinvertebrates present in the sampling areas were also observed. The study was conducted based on reports of mangrove planting activity that disrupted seagrass functions on Santa Fe, Bantayan Island, Cebu. Transect-quadrat method sampling was done to assess the chosen sites. Results: Six species of seagrass was found on the site without mangrove planting which was barangay Ocoy (Cymodocea sp., Thalassia sp., Halodule sp., Enhalus sp., Halophila sp., and Syringodium sp.) and had a higher percent cover, while only four were found on the site with mangrove planting (barangay Marikaban). It was also found that barangay Marikaban had a lesser Shannon-Wiener and Simpson's index compared to barangay Ocoy. Jaccard's index of similarity between the two sites was low. Conclusion: With the results of the assessment, we recommend proper monitoring of future mangrove planting activities and that these activities should not disrupt another ecosystem as all ecosystems are important.

• 한국멀티미디어학회논문지
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• 제19권11호
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• pp.1878-1890
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• 2016

Web 2.0 has features produced the content through the user of the participation and share. The content production activities have became active since social network service appear. The social bookmark, one of social network service, is service that lets users to store useful content and share bookmarked contents between personal users. Unlike Internet search engines such as Google and Naver, the content stored on social bookmark is searched based on tag keyword information and unnecessary information can be excluded. Social bookmark can make users access to selected content. However, quick access to content that users want is difficult job because of the user of the participation and share. Our paper suggests a method recommending search word to be able to access quickly to content. A method is suggested by using Collaborative Filtering and Jaccard similarity coefficient. The performance of suggested system is verified with experiments that compare by 'Delicious' and "Feeltering' with our system.

• Plant Biotechnology Reports
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• 제4권3호
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• pp.237-242
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• 2010

Six parent and their 12 gamma ray-induced somatic flower colour mutants of garden rose were characterized to discriminate the mutants from their respective parents and understanding the genetic diversity using Random amplification of polymorphic DNA (RAPD) markers. Out of 20 primers screened, 14 primers yielded completely identical fragments patterns. The other 7 primers gave highly polymorphic banding patterns among the radiomutants. All the cultivars were identified by using only 7 primers. Moreover, individual mutants were also distinguished by unique RAPD marker bands. Based on the presence or absence of the 48 polymorphic bands, the genetic variations within and among the 18 cultivars were measured. Genetic distance between all 18 cultivars varied from 0.40 to 0.91, as revealed by Jaccard's coefficient matrix. A dendrogram was constructed based on the similarity matrix using the Neighbor Joining Tree method showed three main clusters. The present RAPD analysis can be used not only for estimating genetic diversity present in gamma ray-induced mutants but also for correct identification of mutant/new varieties for their legal protection under plant variety rights.

• 정보관리학회지
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• 제18권2호
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• pp.203-230
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• 2001

본 연구에서는 문헌을 기반으로 한 지식의 자동분류를 위해 최적의 클러스터링 모형을 제시하고자 하였다. 클러스터링 실험을 위해서 신문기사 실험집단과 학술논문 초록 실험집단을 구축하였고, 분류 성능 평가 척도인 WACS를 개발하였다. 분류자질로 사용한 용어의 집합은 다양한 자질 축소 기준을 적용하여 생성하였으며, 다양한 용어 가중치를 사용하였다. 유사계수 공식으로는 코사인 계수와 자카드 계수를 적용하였으며, 클러스터링 알고리즘으로는 비계층적 기법인 완전연결 기법과 계층적 기법인 K-means기법을 각각 사용하였다. 실험 결과 신문기사 원문 집단에서의 성능이 좋았으며, 완전연결 기법의 성능이 K-means 기법보다 높게 나타났다. 역문헌빈도의 적용은 완전연결 클러스터링에서는 긍정적인 효과가 나타났으나, K-means 클러스터링에서는 그렇지 못했다. 분류자질은 전체의 7.66%만 사용하였을 경우에도 성능 저하가 크지 않았으며, K-means 클러스터링에서는 오히려 성능 향상 효과가 있었다.

• Korean Journal of Acupuncture
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• 제33권1호
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• pp.18-32
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• 2016

Objectives : This study aims to analyze the co-occurrence of pathological symptoms and corresponding acupoints as documented by the comprehensive acupuncture and moxibustion records in the classical texts of Far East traditional medicine as an aid to a more efficient understanding of the tacit treatment principles of ancient physicians. Methods : The Classic of Nourishing Life with Acupuncture and Moxibustion(Zhenjiu Zisheng Jing; hereinafter ZZJ) was selected as the primary reference book for the analysis. The pathology-acupoint co-occurrence analysis was performed by applying 4 values of vector space measures(weighted Euclidean distance, Euclidean distance, $Cram\acute{e}r^{\prime}s$ V and Canberra distance), which measure the distance between the observed and expected co-occurrence counts, and 3 values of probabilistic measures(association strength, Fisher's exact test and Jaccard similarity), which measure the probability of observed co-occurrences. Results : The treatment records contained in ZZJ were preprocessed, which yielded 4162 pathology-acupoint sets. Co-occurrence was performed applying 7 different analysis variables, followed by a prediction simulation. The prediction simulation results revealed the Weighted Euclidean distance had the highest prediction rate with 24.32%, followed by Canberra distance(23.14%) and association strength(21.29%). Conclusions : The weighted Euclidean distance among the vector space measures and the association strength among the probabilistic measures were verified to be the most efficient analysis methods in analyzing the correlation between acupoints and pathologies found in the classical medical texts.