• The Korean Journal of Food And Nutrition
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• v.36 no.1
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• pp.58-68
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• 2023

This study investigated the quality characteristics of beyolmijang prepared with corn (steamed and roasted corn) with different pretreatments and simultaneously added starter (Bacillus velezensis JH1). pH decreased, whereas total acidity showed a tendency to increase. The moisture content decreased slightly according to the fermentation period. In color, the L and b values decreased in all samples, whereas the a value showed a tendency to increase significantly. In microorganisms, sample D had more lactic acid bacteria, whereas the mold content was lower. The total polyphenol content was highest at six weeks in sample E, and other samples showed a tendency to decrease over eight weeks. Antioxidant activity increased significantly. In particular, the content in sample E was significantly higher. Reducing sugar showed a tendency to increase as the fermentation period increased. The highest content was found in sample C containing roasted corn at six weeks of fermentation. Amino nitrogen and ammonia nitrogen content increased in all samples. As a result of electronic tongue, sample E prepared with steamed corn, roasted corn, and lactic acid bacteria showed a low salty taste, sour taste, and high umami taste and was considered a good material for the development of byeolmijang.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.37 no.1
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• pp.74-78
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• 2024

We have studied the effects of Ag on the characteristics of Sn₄₈In₅₂Ag_x (wt%) low-melting solders for photovoltaic ribbons. The Sn₄₈In₅₂ (wt%) solder coexisted in the InSn₄ and In₃Sn alloys. Ag atoms added in the solder formed an AgIn₂ alloy by reacting with some part of In atoms, while they did not react with Sn atoms. The addition of Ag atoms in the Sn₄₈In₅₂Ag_x (wt%) solders showed useful results; an increase in peel strength and a decrease in melting temperature. The peel strength of the ribbon plated with the Sn₄₈In₅₂ (wt%) solder was 53.6 N/mm², and that of the Sn₄₈In₅₂Ag₁ (wt%) solder largely increased to 125.1 N/mm². In the meanwhile, the melting temperature of the Sn₄₈In₅₂ (wt%) solder was 119.2℃, and that of the Sn₄₈In₅₂Ag₁ (wt%) solder decreased to 114.0℃.

• Korean Journal of Microbiology
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• v.54 no.1
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• pp.9-17
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• 2018

To investigate the role of bkdR, sigL, yplP, and des genes which were known to be involved in fatty acid synthesis and sensitive at low temperature, deletion mutants of Bacillus subtilis CU1065 and JH642 were constructed. To determine the low temperature sensitivity of these genes, we compared the growth curves of cells at $37^{\circ}C$ and $15^{\circ}C$. At $37^{\circ}C$, wild type and deletion mutants showed almost similar growth but only bkdR deletion strain at $15^{\circ}C$ showed very slow growing compared with wild type. At $15^{\circ}C$ sigL and yplP deletions were somewhat slower or similar to those of wild type strain. Double and triple mutants for bkdR, sigL, yplP deletions were constructed and grown at $20^{\circ}C$ in LB agar to investigate cold sensitive growth. Double or triple deletions including bkdR deletion showed cold sensitive growing. In order to identify more clearly cold sensitive growth, the experiments were carried out under cold shock conditions in which the temperature was lowered from $37^{\circ}C$ to $15^{\circ}C$ at the point of 0.4 optical densities at 600 nm. In these cold shock experiments, only bkdR deletion showed significantly lower growing and additional des deletion increases cold sensitivity. The bkdR activates the bkd operon, which catabolized isoleucine, valine and leucine, amino acids and produce precursors for the synthesis of branched fatty acids. At cold shock growing of bkdR deletion strain, isoleucine recovered cold sensitivity of bkdR deletion but valine did not restore cold sensitivity. Isoleucine is used as a precursor for the synthesis of anteiso-branched fatty acids. On the other hand, valine is used as a precursor for the synthesis of iso-branched fatty acids. This indicates that anteiso-branched fatty acid plays an important role at the cold shock condition.

• Clinical and Experimental Reproductive Medicine
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• v.31 no.4
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• pp.209-215
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• 2004

Objective: Embryonic stem (ES) cells could be differentiated into the specific cell types by alternation of culture condition and modification of gene expression. This study was performed to evaluate the differentiation protocol for mouse and human ES cells to insulin secreting cells. Methods: Undifferentiated mouse (JH-I) and human (Miz-hESI) ES cells were cultured on STO feeder layer, and embryoid bodies (EBs) were formed by suspension culture. For the differentiation, EBs were cultured by sequential system with three stage protocol. The differentiating ES cells were collected and marker gene expressions were analyzed by seIni-quantitative RT-PCR in each stage. Amount of secreted insulin levels in culture media of human ES cells were measured by human insulin specific RIA kit. Results: During the differentiation process of human ES cells, GATA-4, a-fetoprotein, glucose transporter-2 and Ngn-3 expression were increased whereas OctA was decreased progressively. Insulin and albuInin mRNAs were expressed from stage IT in mouse ES cells and from stage III in human ES cells. We detected 3.0~7.9 IlU/rnl secretion of insulin from differentiated human ES cells by in vitro culture for 36 days. Conclusion: The sequential culture system could induce the differentiation of mouse and human ES cells into insulin secreting cells. This is the fIrst report of differentiation of human ES cells into insulin secreting cells by in vitro culture with serum and insulin free medium.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.3 no.1
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• pp.14-21
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• 1993

N-Heterocyclic aromatics (NHA) are widely occurring environmental pollutants formed during the pyrolysis of nitrogen-containing organic chemicals. NAH are found in significant amounts in tobacco condensates, synthetic fuels, polluted river sediment, and effluents from the heating of coal. Following topical application 7H-dibenzo[c, g]carbazole (DBC) induces cancer in liver as well as skin, indicating that dermal exposure can lead to systemic effect. DBC and dibenz[a,j]acridine (DBA) are examples of NHA. The potency of many carcinogenic compounds is related, at least in part, to the efficiency of their biological activation. We undertook studies to determine which initial metabolites lead to the formation of high levels of carcinogen-DNA adducts in vivo. DBC and DBA's, DBA, trans-DBA-1,2-dihydrodiol (DBA-1,2-DHD), trans-DBA-3,4-dihydrodiol (DBA-3,4-DHD), and trans-DBA-5,6-dihydrodiol (DBA-5,6-DHD), were applied to the skin of mice. There were six adducts that were related to DBC application. These addusts were seen in the target organ, liver at high levels, but at very low levels in non-target organs, skin, lung and kidney. In skin, DBA produced two distinct adducts. The same two adducts were seen when DBA-3,4-DHD was applied. In addition the total adduct level elicited by DBA-3,4-DHD higher than that of parent compound. Two adducts were seen when DBA-5,6-DHD was applied, but these were very different from adducts seen with DBA. These results suggested that activation of DBA to DNA-binding compounds in skin includes initial formation of DBA-3,4-DHD.

• Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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• v.4
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• pp.373-394
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• 2001

Bis jetzt haben wir $haupts\"{a}chlich$ verschiedene Probleme an Ort und Stelle des Fremdsprachunterrichts analysiert, und zwar: (a) den > Spracherwerbsmechanismus < die >SMQ< und den > auf jeweilige (Fremd)sprache fuhrenden Unterrieht<, die drei Lehr- u. Lernfaktoren des Spraeherwerbs, (b) den Lehr- u. $Lemproze\ss$ der Muttersprache, (c) die 5 Fertigkeitsaufgaben und (d) einige Prinzipien und ihre $Ma{\ss}nahmen$ der Sprachlehr- u. -lernmethode Aus diesen Grundlagen werden die folgenden Ergebnisse be- sonders hervorgebracht werden: (d) Das Hauptziel des Fremdspraehunterrichts soll/muss man darin finden, $zun\"{a}chst$ die >SMQ< zu $erf\"{u}llen$, die etwa 1000 $\"{A}u{\ss}erungen/S\"{a}tzen$ entspricht, um perfekt zu sprechen. (e) Alle Menschen kommen ausnahmslos genetisch mit dem >Spracherwerbsmechanismus< zur Welt, der alles Sprachmaterial grammatisch/systematisch aufnimmt. (f) Aus diesem Grund darf man den $Anf\"{a}nger$ keine Gramma -tik lehren, bis er perfekt sprechen kann, weil er als Mensch schon alles grammatisch lernen kann. (g) Stattdessen lohnt es sich, im Unterricht nur auf jeweilige Fremdsprache zu sprechen, zu schreiben und zu $erkl\"{a}ren$, d.h. den auf jeweilige Sprache $f\"{u}hrenden$ Unterricht intensiv zu hal- ten, was auch beim Mutterspracherwerb immer der Fall ist. (h) Es ist sehr ratsam $f\"{u}r$ uns, die Lehr- u. Lernmethode im Fall des Mutterspracherwerbs als Unterrichtsmodell dynamisch anzuwenden, weil sie uns immer den $100\%$ Erfolg geschenkt hat. (i) Im Alltag braucht man $st\"{a}ndig$ den Ansprecher/die -in (z.B. im Fernseh/ durch Internet) nachzuahmen, und zwar die Sprechweise, die Gestik bzw. Mimik usw. (j) Es soll/muss streng verboten werden, im Fremdsprach- unterricht auf Muttersprache zu sprechen, weil es mit der Fremd -sprachausbildung, d.h. mit der $Erf\"{u}llung$ von >SMQ< gar nicht zu tun hat, sondern eine Art Betrug sein $k\"{o}nnte$, soweit das folgende koreanische Sprichwort noch gilt: Der Apfel $f\"{a}llt$ nicht weit vom Stamm. (k) Ohne Reformierung des konventionellen Fremdsprachunter -richts, $100\%\;Mi{\ss}lingen$ hinnehmen zu $m\"{u}ssen$, kann man nichts weiter machen. Denn er $st\"{o}rt$ uns das auf jeweilige Sprache bezo -gene Denken. Z.B. die sinnlose Grammatik, auf Muttersprache zu sprechen, die unmittelbare $\"{U}bersetzung$ usw. sind dessen $R\"{a}delsf\"{u}hrer.\;Alle\;L\"{a}nder\;besch\"{a}ftigen\;sich\;st\"{a}ndig\;damit,\;ihre\;M\"{o}glichkeit$ in der harten Existenzkonkurrenz durch die Bildungsreform auszu -gleichen. Denn die Ausbildung der Hochtechnologie im 21. Jh. braucht notwendig die $Fremdsprachf\"{a}higkeit$ als ihre Grundlage. Ohne Selbstreformierung $k\"{o}nnten$ wir als Fremdsprachlehrer/-pro -fessoren - auf Koreanisch sprechend - weiter den Fremdsprach -unterricht halten?

• The Journal of Korean Physical Therapy
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• v.13 no.3
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• pp.585-596
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• 2001

The twenty one individuals with total knee replacement who were admitted to Kang-Dong Hospital for comprehensive physical therapy were studied in order to demonstrate the effectiveness of an isokinetic test program using the Cybex 6000 machine. The subjects were divided into three groups with the isometric exercise group receiving isometric exercise, the isokinetic eccentric exercise group, and isokinetic concentric exercise group receivind isokinetic exercise(eccentric, concentric) to knee flexors and extensors muscles for a six weeks' period using the Cybex 6000. The results are follow: 1. The extensors were increased significantly at all groups after 6 weeks training(p<.05). The flexors were increased significantly at isokinetic eccentric and isokinetic concentric group but no significantly differences at isometric group(p>.05) 2. At the effect of extensors and flexors after 6 weeks training. higher to 30$^{\circ}$ /sec of isokinetic concentric exercise, lower to 120$^{\circ}$ /sec of isometric exercise. 3. The peak torque was more increased significantly in the flexors and extensors of the isokinetic conccentric exercise among three groups. 4. The total work was more increased significantly in the flexors and extensors of the isokinetic conccentric exercise among three groups. 5. The ratio of peak torque to body weight were more increased significantly in the flexors and extensors of the isikinetic conccentric exercise among three groups. 6. The average power was more increased significantly in the flexors and extensors of the isikinetic conccentric exercise among three groups. 7. The average R.O.M in the pre-exercise and post-exercise was not different significantly in all three groups. According to the above results, In the muscle strength recovery for total knee replacement patients, isokinetic concentric exercise group was significantly greater than the isokinetic eccentric and isometric exercise groups after a six weeks training.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.339-339
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• 2017

The long grain rice varieties adaptable to South-east Asia tropical regions were tried to develop in Cambodian Agriculture Research and Development Institute (CARDI), Cambodia. The final goal is to develop rice varieties which can culture in diverse environmental conditions of tropical regions of South-east Asia under climate change. We collected and evaluated for agronomic traits of 131 rice germplasm from Cambodia, China, India, Indonesia, Myanmar, Philippines and Vietnam in CARDI. We selected core germplasm including leading varieties of target countries and made 813 F1 cross combinations between leading varieties of each country and promising germplasm of high yield potential, resistance to biotic/abiotic stresses, aromatic rice, and so on. Out of 607 F1s evaluated to heading date, plant type, agronomic traits, and grain type, 106 F1s selected and advanced to F2 populations. 106 F2 populations were evaluated to major agronomic traits, grain type and yield-component traits, and selected 2,560 plants in 62 F2 populations. During six seasons in 2014~2016, the lines of F3 subsequent-generation were cultured a total of 6,256 lines. In yield trial for promising lines in F5 generation, the growth duration from sowing to harvesting was 97~114 days. These lines were 88~129 in number of grain per panicle, an average of 84.6% in the range of 79.3~91.9% in the percentage of ripened grain and 17.5~22.8g in 1000-brown rice weight. The rough rice yields were in the range of 4.33~6.06 ton/ha with an average of 5.23 ton/ha. The yield was increased to 5~47% than Chulsa and 12~41% than IR66. Five lines, KR54-28-1, KR55-14-2, KR57-5-2, KR67-57-2 and KR128-19-1 were 5.33~6.06 ton/ha in rough rice yield. These high yield potential lines would be evaluated to adaptability in Cambodia, Laos, Myanmar and Vietnam during 2017.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.155-155
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• 2017

The long grain rice varieties adaptable to South-east Asia tropical regions were tried to develop in Cambodian Agriculture Research and Development Institute (CARDI), Cambodia. The final goal is to develop rice varieties which can culture in diverse environmental conditions of tropical regions of South-east Asia under climate change. We collected and evaluated for agronomic traits of 131 rice germplasm from Cambodia, China, India, Indonesia, Myanmar, Philippines and Vietnam in CARDI. We selected core germplasm including leading varieties of target countries and made 813 F1 cross combinations between leading varieties of each country and promising germplasm of high yield potential, resistance to biotic/abiotic stresses, aromatic rice, and so on. Out of 607 F1s evaluated to heading date, plant type, agronomic traits, and grain type, 106 F1s selected and advanced to F2 populations. 106 F2 populations were evaluated to major agronomic traits, grain type and yield-component traits, and selected 2,560 plants in 62 F2 populations. During six seasons in 2014~2016, the lines of F3 subsequent-generation were cultured a total of 6,256 lines. In yield trial for promising lines in F5 generation, the growth duration from sowing to harvesting was 97~114 days. These lines were 88~129 in number of grain per panicle, an average of 84.6% in the range of 79.3~91.9% in the percentage of ripened grain and 17.5~22.8g in 1000-brown rice weight. The rough rice yields were in the range of 4.33~6.06 ton/ha with an average of 5.23 ton/ha. The yield was increased to 5~47% than Chulsa and 12~41% than IR66. Five lines, KR54-28-1, KR55-14-2, KR57-5-2, KR67-57-2 and KR128-19-1 were 5.33~6.06 ton/ha in rough rice yield. These high yield potential lines would be evaluated to adaptability in Cambodia, Laos, Myanmar and Vietnam during 2017.

• International Journal of Advanced Culture Technology
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• v.6 no.3
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• pp.59-68
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• 2018

This study was a cross-sectional descriptive study of the effects of emotional happiness on aging preparation for 45-60 year olds. Participants of this study were 312 volunteers and data was collected online, the collection period was from May 15, 2018 to May 31, 2018. The tools used in the research were the emotional happiness developed by Watson et al (1988) et al., the aging preparation developed by JH Lee (2009). Analysis was done using t-test, ANOVA, Pearson correlation, and regression by SPSS 18.0. As a result of the analysis, 84.0% of participants felt physically healthy and 89.8% felt mental health. The spouses of participants were physically 83.4% healthy and mentally 82.7% healthy. The correlation between emotional happiness, health cognition and aging preparation showed that physical health had a correlation with mental health (r = .482, p <0.01) and emotional happiness (r = .369, p <0.01), economic aging preparation (r = .411, p <0.01), social aging preparation (r = .119, p <0.01). Mental health was correlated with emotional happiness (r = .491, p <0.01), economic aging preparation (r = .411, p <0.01) and social aging preparation. Difference between emotional happiness and retirement preparation according to presence or absence of disease, emotional happiness (t = -4.503, p <0.01) and economic aging preparation (t = -4.960, p <0.01) were statistically significant. Emotional happiness affects the preparation of old age, emotional happiness affects to physical aging preparation under statistical significance (F=25.191, p<0.01), economic aging preparation (F=1131.783, p<0.01), and social aging preparation (F=147.672, p<0.01) which were sub components of aging preparation. Emotional happiness showed a 7.5% effect on the preparation of physical aging, 78.4% on economic aging preparation and 32.3% on social aging preparation. Based on the above results, it can be concluded that physical health and mental health are related to emotional happiness, economic aging preparation, and social aging preparation. And also these results can be inferred that the illness causes emotional less feeling of happiness and economic difficulty. Based on the results of this study, emotional well - being influences the retirement preparation significantly at statistical significance. Therefore, if the concept of emotional happiness is introduced to the old-age preparation program, it will be more client centered program.