• Reproductive and Developmental Biology
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• v.29 no.3
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• pp.169-174
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• 2005

This study was comducted to examine the effects of culture medium, and the osmolarity and osmotic change of the culture medium on in vitro maturation of porcine oocytes and developement of porcine parthenogenetic embryos. In Experiment 1, cumulus-oocyte complexes were matured in NCSU-23, mWM and mKRB, respectively, There was no difference in maturation rate($62.1\~71.3\%$) among groups. In Experiment 2, matured oocytes in each medium were activated and cultured for 6 days in the same media. Blastocyst formation rate was higher in NCSU-23($22.9\%$) than those of others($0\~0.6\%$, P<0.05). In Experiment 3, parthenogenetic embryos were cultured for 6 days in NCSU-23 with different osmolarity(300, 280 and 256 mOsmols) adjusted by NaCl. There were no differences in development rates to the blastocyst stage($11.0\~14.4\%$) among groups. In Experiment 4, activated oocytes were cultured for 2 days in NCSU-23 with 300, 280 and 256 mOsmols and then transferred to increased or decreased osmotic condition. Blastocyst formation rate was higher in a group which was transferred from the higher osmotic condition to the lowe. osmotic condition($21.0\%$) than a contrary group( $11.8\%$, (P<0.05). This result shows that the culture medium and the osmolarity of the culture medium affect the development of porcine parthenogenetic embryos, and the change of osmolarity from the higher condition to the lower condition at a certain developmental stage can enhance the development of porcine parthenogenetic embryos.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.133-140
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• 1999

This pot experiment was conducted to find out the forage productivity and quality in a grass/clover sward as affected by the application of three different fertilizers; double superphosphate(DS), fused Mg-phosphate(FP), and complex fertilizer(CF), on newly reclaimed hilly soil. This 1st part was concerned with the forage yields, yield components and botanical composition. The results obtained are summarized as follows: 1. The significant differences in the yields(relative yields, %) of whole forages and legume were laid in the following increasing order; CF(relative yield: 150.4% in whole forages and 169.7% in ladino clover) > FP(125.4% in whole forages and 121.7% in ladino clover) > DS(100.0%). The yield of orchardgrass was significantly reduced in the DS plot, compared with the yields in the FP and CF plots, whereas these showed no significant differences between the FP and CF plots. 2. In the botanicl composition, it showed that the rates of grass : legume were 48:52% in the DS and FP, and 40:60% in the CF. It was recognized that the application of complex fertilizer contributed to the increasing of the yields of whole forages, and especially legume yield and rate in the mixed sward.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.4
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• pp.231-238
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• 2007

In the previous study, the anti-oxidant activity of extract/fraction of Sueada asparagoides (SA) was investigated and the results showed that the ethylacetate (EtOAc) fraction and its aglycone fraction had the best performance on the free radical scavenging activity, reactive oxygen species scavenging (ROS) activity and cell protective activity (J. Soc. Cosme. Scientists Korea, 33(3), 145 (2007)). In this study, the stability of cream containing 0.3% SA EtOAc extract (called extract below) was evaluated. pH, viscosity and absorbance (363 nm) were measured under the 4 different temperatures ($0^{\circ}C,\;25{\circ}C,\;37{\circ}C\;and\;45{\circ}C$) and under the sun light at the 4 week intervals during the 12 weeks in total. The control cream without containing the extract did not show pH change under the different temperatures mentioned above. However, the pH of the cream the extract was decreased 0.08 at the temperature ranges of $0^{\circ}C\;to\;37^{\circ}C$. Under the $45^{\circ}C$ and sun light condition, the pH was decreased 0.51 and 0.66, respectively. The cream containing the extract did not show absorbance change at the temperature ranges of 0 to $37^{\circ}C$ for 12 weeks. Instead, the absorbance of the cream treated under $45^{\circ}C$ and sun light condition was decreased 7.6 % and 7.4 %, respectively. This decrease in absorbance is relatively small compared to the 48.3 % decrease of the extract sampled from the cream using ethanol solution. This indicates that the extract is stabilized in the cream. After treating the cream for 12 weeks under the different temperatures, the viscosity was measured for the cream containing the extract and control cream. The values were increased by 1,748 cPs in average compared to the initial value for the former and by 951 cPs in average for the latter. On the other hand, the viscosity of control cream treated under the sun light for 12 weeks was significantly decreased (4,022 cPs) relative to the cream containing the extract, which showed 2,484 cPs increase in viscosity. This indicates that the SA extract contributes to the stability of the emulsion product by protective effect to maintain the viscosity of the cream against sun light. In addition, any change in color or smell was not observed through 12 weeks of the experimental time period. Thus, it is concluded that it is still not clear in the stability of the cream containing the extract when it is stored for the long time. Accordingly, it is suggested that further study is needed to provide more information to the manufactures, who are seeking for the application of the extract to improve the anti-oxidant activity and stability of cosmetic products.

• Korean Journal of Weed Science
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• v.17 no.1
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• pp.31-43
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• 1997

This study was carried out to investigate classfication of weedy rice (Oryza sativa) based on isozymes esterase and peroxidase, growth and developmental difference of weedy rices and rices grown under dry and water condition, and weedy rice control and tolerant difference of weedy rices in various herbicides using weedy rices collected from thirteen strains of Chonnam, one Chonbuk, two Kyeongki and two rice cultivars. 1. The collected weedy rices were classified into three groups based on isozyme esterase and peroxidase using polyacrylamide gel electrophoresis(PAGE) method. The classified groups were not same each other. 2. Plant height was taller in collected weedy rices than rice cultivars at 18 days after seeding under dry and water conditions, but number of leaves, shoot fresh weight, root fresh weight and root length were not significantly different between collected weedy rices and rice cultivars. In addition, growths of collected weedy rices were greater in dry- than water-condition. 3. After thiobencarb(S-4-chlorobenzyl diethythiocarbamate), molinate(S-ethyl hexahydro-1H-azepine-1-carbothioate) and oxadiazon(5-tert-butyl-3(2,4-dichloro-5-isopropoxyphenyl)-1,3,4-oxadiazol-2-one) were applied at 6 days before seeding, the weedy rices controlled 100% by thiobencarb at 2.1kg ai/ha and 024kg ai/ha oxadiazon treatment but controlled 26% to 67% by molinate at 6.5kg ai/ha. Rice due to the herbicides was injured severely(25% to 100%) in flood condition at time of rice seeding after oxadiazon at 0.48kg ai/ha and 2.1kg ai/ha thiobencarb application, except for molinate which injured rice slightly(4% to 13%) in drain condition. The collected weedy rices to all experimented herbicides showed slight intraspecific variations. The intraspecific variations of weedy rices decreased in the order of thiobencarb>molinate>oxadiazon.

• Korean journal of applied entomology
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• v.10 no.2
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• pp.109-116
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• 1971

The study involved determination of resistance levels of spider mites to Metasystox, Folidol and C-8514 using slide dip technique: a feid trial of alternative acaricides using an O/P resistant strain. 1. Resistant strains of two-spotted spider mite( Tetranychus urticae Koch) were collected from Taegu, Kyungsan, Chunchun, Yesan, Suwon, Osan and Chungju, and kept in the glasshouse through the experiments. Resistant strains of European red mite (Panonychus ulmi Koch) were collected from Chungju, Taegu, Yesan, Chunchun, Suwon and Whasung, and Wonju susceptible was collected. Immediately after collection, tests were applied. All strains except susceptible populations had regularly been sprayed with Organo-phosphates including Parathion (or Folidol), Metasystox, Phenkapton and Trithion for more than the ten Bast years; and Organo-chlorines such as Kelthane and C-8514 more than five years. Comparisons of the resistant strains and susceptible strains at the $LC_50$ values are as follows: a. With Metasystox, resistance levels of the Chungju, Taegu, Yesan, Chunchun and Suwon resistant strains of P. ulmi were respectively, 96,52,4,3 and 2, times as resistant as the Wonju susceptible strain. b. With Folidol, resistance levels of the Chungju, Chunchun, Yesan, Whasung and Suwon strains of P. ulmi showed respectively, 126, 48, 33, 30 and 6-fold resistance levels over the susceptible strain. c. With C-8514, resistance levels of the Taegu, Suwon, Whasung and Chungju strains of P. ulmi were 42, 31, 30 and 20 times greater than the susceptible strain, respectively. d. With Metasystox, resistance levels of the Taegu, Chunchun, Yesan and Suwon, strains of T urticae were respectively, 32,29,25 and 17 times as resistant as the susceptible strain. e. With C-8514, resistance levels of the Taegu, Chungju and Kyungsan strains of T. urticae showed respectively, 59, 29 and 19-fold resistance levels over the Osan strain. 2. Field trials were carried out to assess the toxicities of eleven alternative materials which would be used for control of O/P resistant strain of Panonychus ulmi. The acaricide groups represented were 2 Organo-chlorines (Kelthane MF and Prethylen), 1 carbamate (Furadan), 1 nitrophenyl (Morocide), 1 carbonate (Morestan), 1 tin(Plictran), 1 fluorine (Nissol), 2 mixtures (Fundal and Banmite) and Organo-phosphate (Phenkapton). Of all acaricides tested Kelthane, Plictran, Fundal and Morestan were the most effective; fol]owed by Banmite, Furadan, Prethylen and Nissol. Sumite and Morocide were intermediate, but Phenkapton(Organo-phosphate) was very poor. In other words, a first application of the above outstanding materials gave very high degrees of control of O/P resistant population of European red mite for about 7 to 12 days. However, the results indicate that secondary application would sometimes be necessary. There was no foliage damage to apple trees by any of the acaricides tested of the concentrations used.

• Research in Plant Disease
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• v.22 no.4
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• pp.227-235
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• 2016

Plant disease caused by root-knot nematode is a major problem in crop production. Using of chemical pesticides, one of the most efficient methods to control nematodes, have raised issues in toxicity to humans and animals and environmental pollution. In this study, to select actinomycete strains that have potential to serve as a microbial agent for control of nematodes, we investigated nematicidal activity of culture broth from 670 Streptomyces isolates. A culture filtrate of KRA15-528 isolate that was identified as S. flavogriseus on the basis of 16S rRNA sequence analysis, showed strong nematicidal activity against second stage of juveniles of Meloidogyne incognita and inhibited egg hatching; exposure to 10% of culture filtrate resulted in 71% juvenile mortality at 48 hours afters treatment and suppressed egg hatching by 54% at 9 days after treatment. When the KRA15-528 culture filtrate was partitioned with ethyl acetate and butanol, ethyl acetate layer exclusively showed strong activity; 91%, 53%, 30% of mortality at 1,000, 500, $250{\mu}g/ml$, respectively. Additionally, the culture filtrate suppressed gall formation on cucumber plant by M. incognita with no phytotoxicity. These results suggest that S. flavogriseus KRA15-528 has potential to serve as a microbial nematicide for the control of root-knot nematode disease.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.3
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• pp.243-250
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• 1981

Soybean, azuki-bean and mungbean were cultivated at different growing conditions, May 10, June 9, July 9 seeding, and May 10 seeding-short day treatment, to investigate the distribution of flowering date and the variation of pod/flower ratio. The growth habit type of the soybean variety used was determinate, but the azuki-bean and mungbean varieties used showed indeterminate growth habit. The pod/flower ratio ranged 38 to 48% for soybean, 23 to 34% for azuki-bean and 46 to 60% for mungbean along with growing conditions. Flower abscission for soybean and mungbean, and pod abscission for azuki-bean affected more on the number of matured pods. Soybean showed once full blooming period, but azuki-bean and mungbean showed two or three times full blooming period except July 9 seeding plot through the whole growing period. Flowers bloomed just after every full blooming period showed lower value of pod/flower ratio and higher pod/flower ratio was obtained from flowers bloomed before or far after full blooming at plots seeded May 10 and June 9 in three pulse crops. Most full blooming period of soybean coincided within 5 days near the terminal leaf appearance date, and number of flowers bloomed before terminal leaf expansion and matured pods from these flowers were major in every soybean plant at all growing codition plots. No relations were found between terminal leaf appearance and flowering or pod setting patterns of azuki-bean and mungbean.

• Korean Journal of Poultry Science
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• v.34 no.2
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• pp.91-97
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• 2007

To evaluate the physiological status of laying flocks, the blood chemistry values were measured and analyzed in various ages under different feeding conditions. Total 671 birds from 48 Hyline brown hens flocks from 13 different poultry farms were bled at the ages of day(s) 1, 11, 21, 50, 80, 120, 180, 240, 300, 400, and 500. The 17 blood chemistries including glucose, lipids, proteins, enzymes, electrolytes and metabolic by-products were measured with an autoanalyzer. Blood glucose showed the highest at the hatching day not relate with the dietary carbohydrates and energy, but tended to decrease during the rest of growth stage in hens. Total blood protein, albumin and globulin increased depending on the ages even though dietary protein was decreased. Blood lipid was greatly changed at different growth stages. Cholesterol was the highest at hatching period and maintained consistently until the 120 days of age. It was increased in birds after 180 days of age. HDL was also highest in hatchery, but decreased greatly after 180 days of age. However, TG was the lowest at one day old, but was increased up to 10 times after 180 days of age compared to that of one day old. The enzyme activities were different. AST, ALT, and GGT showed comparatively contained consistently, whereas amylase was slowly decreased. Blood P, Na, K and Cl showed consistency, but Ca content was increased upto two times of the one day of age. The results from this study showed that the blood chemistry values were affected by the general metabolic status of the host with ages not by feeding conditions. Further, the standard data of age-dependent blood chemistry values in the laying flocks were obtained, which can be utilized for early detection of the changes in the physiological status occurred by the infectious or metabolic diseases. The results of these analyses seemed to be useful to increase the productivity of laying flocks through rapid and proper veterinary medical treatments.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.4
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• pp.220-227
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• 2004

Phosphorus desorption study is essential to understanding P behavior in agricultural and environmental soils because phosphorus is considered as two different aspects, a plant nutrient versus an environmental contaminant. This study was conducted to determine soil P buffering power related to P desorption quantity intensity (Q/I) parameters, $Q_{max}$(an index of P release capacity) and $l_0$(an index of the intensity factor), and to investigate the characteristics of relationship between the P desorption Q/I parameters and the soil properties. Soil samples were prepared with treatments of 0 and $100mg\;P\;kg^{-1}$ applied as $KH_2PO_4$ solution. The P desorption Q/I curves were obtained by a procedure using anion exchange resin beads and described by an empirical equation ($Q=aI^{-1}+bln(I+1)+c$). The P desorption Q/I curves for the high available P (${\g}20mg\;kg^{-1}$ of Olsen P) soils were characteristic concave trends with or without soil P enrichment, whereas for the low available P (${\lt}20mg\;kg^{-1}$ of Olsen P) soils, the anticipated Q/I concave curves could not be obtained without a proper amount of P addition. When the soils were enriched in phosphates, the values of desorbed solid phase labile P and solution P, such as $Q_{max}$ and $I_0$ respectively, were increased, but the ratio of $Q_{max}$ versus $I_0$ was decreased. Thus, the slope of desorption Q/I curve represented as phosphorus buffering power, $|BP_0|$, is decreased. The $|BP_0|$ values of the high available P soils ranged between 48 and $61L\;kg^{-1}$ in the P untreated samples and between 18 and $44L\;kg^{-1}$ in the P enriched samples. Overall $|BP_0|$ values of both low and high available P soils treated with $l00mg\;P\;kg^{-1}$ ranged between 14 and $79L\;kg^{-1}$. The $Q_{max}$, values ranged between 71.4 and $173.1mg\;P\;kg^{-1}$, and the lo values ranged between 0.98 and $3.82mg\;P\;L^{-1}$ in the P enriched soils. The $Q_{max}$ and $I_0$ values that control the P buffering power may be not specifically related to a specific soil property, but those values were complicatedly related to soil pH, clay content, soil organic matter content, and lime. Also, phosphorus release activity, however, markedly depended on the desorbability of the applied P as well as the native labile P.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.625-636
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• 2005

This study was conducted to determine effects of different ratios (3.5, 3.0 and 2.5) of nonfibrous carbohydrate (NFC) to ruminally degradable protein (RDP) on in vitro fermentation and lactation performance of dairy cows and optimum ratio of NFC to RDP in dairy rations. In vitro trial was conducted up to 12 hr with ruminal fluidtaken from ruminally cannulated Holstein cows. The level of dietary NFC did not affect ruminal pH. The ammonia-N concentration was not significantly different among treatments until 6 hr incubation, however, it was significantly (P < 0.05) decreased as the ratio of dietary NFC to RDP increased on 9 and 12 hr incubation. For volatile fatty acids, concentrations of both acetate and propionate were significantly (P < 0.05) increased on 3 hr incubation as dietary NFC contents of treatments increased, in other incubation times, they had no significant differences among treatments. Valerate and A:P ratio were not affected by the ratio of NFC to RDP. Isoacids and total VFAs were significantly (P < 0.05) increased with increasing dietary NFC contents and their values were highest in the treatment of 3.0 ratio. Meanwhile, for in vivo trial, 18 Holstein lactating cows were allotted to treatments in three groups of 6 cows. They were employed for 24 weeks to investigate nutrient intakes, and milk yield and composition according to different ratios of dietary NFC to RDP. Intakes of dry matter and energy were significantly (P < 0.01) increased, but NDF intake was significantly (P < 0.01) decreased as the ratio of dietary NFC to RDP increased. Milk yield for the ratio of 3.5 (32.7 kg) was significantly (P < 0.05) higher than those of other treatments. Milk fat (%) was significantly (P < 0.05) higher for the treatments of 3.0 (3.79 %) and 2.5 (3.79 %) than that (3.48 %) for the ratio of 3.5, but milk fat yield was not different among treatments. Contents and yields for milk protein and solids-not fat were linearly (P < 0.01) increased as the ratio of dietary NFC to RDP increased. However, milk urea nitrogen concentration was significantly (P < 0.05) decreased with increasing dietary NFC levels. Our results showed that the increasing level of NFC in the diet of dairy cows enhanced ruminal fermentation, N utilization and milk production and suggested that maximal fermentation and lactation performance were achieved when the dietary ratio of NFC to RDP was more than 3.0 in dairy rations.