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New Antisense RNA Systems Targeted Against Plant Pathogens

  • Matousek, J.;Vrba, L.;Kuchar, M.;Pavingerova, D.;Orctova, L.;Ptacek, J.;Schubert, J.;Steger, G.;Beier, H.;Riesner, D.
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.5
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    • pp.379-385
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    • 2000
  • tRNA and 7SL RNA based antisense vehicles were prepared by inserting conserved anti-viral and anti-viroid domains. Anti-PVS coat protein leader sequence (ACPL) and antistructural antihairpin domain of PSTVd (AHII) were inserted in tRNA cassette; anti- zing finger domain of PVS, AHII and anti hop latent viroid ribozyme were inserted in 7SL RNA gene isolated from A. thaliana. These constructs were shown to be transcribed both, in in vitro and in in vivo conditions. However, it followed from our work that closely linked position of PoIII reference genes and PoIIII antisense genes within T-DNA lead to the impairment of RNA expression in transgenic plants. To assay in vivo transcription of antisense genes, hairy root potato cultures were established using h. tumefaciens A4-24 bearing both, Ri plasmid and PoIII-promoterless plant expression vectors with antisense RNA genes. Expression of antisense RNA in transgenic potato tissues was proven by specific RT-PCR reactions.

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EFFECTS OF THE VEHICLE MODEL ON SHIFTING TRANSIENTS OF PASSENGER CARS WITH AUTOMATIC TRANSMISSION

  • Kong, J.H.;Park, J.H.;Lim, W.S.;Park, Y.I.;Lee, J.M.
    • International Journal of Automotive Technology
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    • v.7 no.2
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    • pp.155-160
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    • 2006
  • This paper presents a vehicle model for analyzing the transient shifting characterisitics of a passenger car with automatic transmission. Then the presented vehicle model was linked with the dynamic model of an automatic transmission. In order to identify the parameters of the vehicle model, we installed a test equipment with an accelerometer in a conventional vehicle and performed road tests. With the proposed vehicle model, we simulated the dynamic characteristics during shifting, and benchmarked with experimental results. Moreover, a modal analysis was carried out to investigate the effect of the vehicle model in the frequency domain and to obtain the transfer function of the vehicle model. In addition, we showed the numerical results in the time domain for analyzing the effect of each stiffness element, such as engine mountings and suspensions.

Shape anisotropy and magnetic properties of Co/Ni anti-dot arrays

  • Deshpande, N.G.;Seo, M.S.;Kim, J.M.;Lee, S.J.;Lee, Y.P.;Rhee, J.Y.;Kim, K.W.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.444-444
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    • 2011
  • Recently, patterned magnetic films and elements attract a wide interest due to their technological potentials in ultrahigh-density magnetic recording and spintronic devices. Among those patterned magnetic structures, magnetic anti-dot patterning induces a strong shape anisotropy in the film, which can control the magnetic properties such as coercivity, permeability, magnetization reversal process, and magneto-resistance. While majority of the previous works have been concentrated on anti-dot arrays with a single magnetic layer, there has been little work on multilayered anti-dot arrays. In this work, we report on study of the magnetic properties of bilayered anti-dot system consisting of upper perforated Co layer of 40 nm and lower continuous Ni layer of 5 nm thick, fabricated by photolithography and wet-etching processes. The magnetic hysteresis (M-H) loops were measured with a superconducting-quantum-interference-device (SQUID) magnetometer (Quantum Design: MPMS). For comparison, investigations on continuous Co thin film and single-layer Co anti-dot arrays were also performed. The magnetic-domain configuration has been measured by using a magnetic force microscope (PSIA: XE-100) equipped with magnetic tips (Nanosensors). An external electromagnet was employed while obtaining the MFM images. The MFM images revealed well-defined periodic domain networks which arise owing to the anisotropies such as magnetic uniaxial anisotropy, configurational anisotropy, etc. The inclusion of holes in a uniform magnetic film and the insertion of a uniform thin Ni layer, drastically affected the coercivity as compared with single Co anti-dot array, without severely affecting the saturation magnetization ($M_s$). The observed changes in the magnetic properties are closely related to the patterning that hinders the domain-wall motion as well as to the magneto-anisotropic bilayer structure.

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Effective Analysis of Incremental Forming Process using the Automatic Expansion of Domain Scheme (자동 영역확장법을 이용한 점진 성형공정의 효율적 해석)

  • Lee K.H.;Lee S.R.;Hong J.T.;Yang D.Y.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.812-815
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    • 2005
  • The incremental forming process employs several tens or hundreds of continuous local strokes, so the entire process is difficult to analyze due to much computation time and large computer memory. The objective of this work is to propose a new numerical scheme of the finite element method, automatic expansion of domain (AED), and to reduce computation time and computer memory. In the AED scheme, an effective analysis domain in each local forming step is defined and then the domain is automatically expanded in accordance with the repeated process. In order to verify the validity of the criterion for the AED scheme and the applicability of the AED scheme, two-dimensional incremental plane-strain forging process is first analyzed using the proposed scheme with various criteria and full domain. In addition, three-dimensional incremental radial forging process is analyzed to verify the applicability of the proposed scheme to a practical incremental forging process.

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A Study of Numerical Wave Tank for 3-Dimensional Free Surface Wave Simulation (3차원 자유표면파 모사를 위한 수치 파수조에 관한 연구)

  • Ha, Y.R.;Kim, Y.J.
    • Journal of Power System Engineering
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    • v.15 no.6
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    • pp.27-34
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    • 2011
  • The increasing capabilities of the computers enable us to utilize various numerical schemes for the time-domain simulations concerned with 3-dimensional free-surface wave problems. There are still difficulties to solve such kind of problems, however. That's because long time simulations with large computational domain are needed in time-domain analysis. So, we need faster and more efficient numerical schemes to get the solutions practically for these problems. In this paper, a high-order spectral/boundary-element method is used for the numerical investigation of physics involved in wave-body interaction. This method is one of the most efficient numerical methods by which the nonlinear gravity waves can be simulated and hydrodynamic forces also can be calculated in time-domain. To get the robust study in these topics, various numerical tests are performed and compared with others' works.