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Phylogenetic Analysis of Native Vigna sinensis in Korea Using DNA Sequence of Internal Transcribed spacer (ITS) Region (토종 갓끈동부의 ITS1, 5.8S 및 ITS2의 염기서열을 이용한 계통 분석)

  • Seo, Pil-Soo;Lee, Sook-Young;Shin, Yong Kook
    • Journal of Life Science
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    • v.27 no.3
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    • pp.351-354
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    • 2017

  • Cowpea (Vigna unguiculata (L.) Walp.) is recognized as a potential source of protein and other nutrients. The genus Vigna includes 100 wild species of plants. Especially, Vigna unguiculata includes annual cowpeas (ssp. unguiculata) and ten wild perennial subspecies. DNA sequence of internal transcribed spacer (ITS) region was determined for Vigna sinensis, one of native plant, which was found in recent but thought to have gone extinct in Korea. The seeds of Vigna sinensis used in this study were donated from Dong-Young Jo. The DNA sequence of ITS-5.8S-ITS2 for Vigna sinensis obtained from this study was deposited as Vigna sinensis AY195581 on GenBank of NCBI (National Center for Biotechnology Information). We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. DNA similarities among subspecies including Vigna unguiculata showed the range 98 to 100% in sequence-based phylogenetic analysis using total 507 base pairs of ITS1, 5.8S and ITS2. Vigna unguiculata and subspecies were grouped independently as one cluster from other Vigna species used in the phylogenetic analysis. In this study, based on the phylogenetic analysis using the ITS1-5.8S-ITS2 sequence of Vigna sinensis, it may be concluded to be classified to one of Vigna unguiculata substrains.

  • https://doi.org/10.5352/JLS.2017.27.3.351 인용 PDF KSCI

A phylogenetic analysis of Korean Artemisia L. based on ITS sequences (ITS 염기서열에 의한 한국산 쑥속(Artemisia L.)의 계통분류학적 연구)

  • Lee, Jeong-Hoon;Park, Chung-Berm;Park, Chun-Geon;Moon, Sung-Gi
    • Korean Journal of Plant Resources
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    • v.23 no.4
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    • pp.293-302
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    • 2010

  • Taxa of Artemisia collected in Korea were constructed by molecular phylogenetic analysis based on the internal transcribed spacer(ITS) regions of nrDNA. The length of the ITS sequences aligned using the clustal X program was 636~643 bp, and the lengths of the ITS1 and ITS2 regions were 251~255 bp and 217~222 bp, respectively. The total number of variable sites was 95 for the entire sequence, and a parsimony- informative site represented an efficacious site in ITS1 rather than in ITS2. The maximum parsimony tree as calculated by the MEGA 4 program was clustered into five clades. The taxa(A. capillaris, A. japonica var. japonica, A. japonica var. hallaisanensis, A. japonica subsp. littoricora) degenerated ovary of clade 1 was supported as the subgenus Dracunculus by Ling's classification system. The results show that A. nakaii and A. fukudo were quite similar genetically(Boostrap 99%) and that the scientific name of Korean A. dubia should be reconsidered. A. sp. distributed in Ganghwa province was grouped with A. argyi(Boostrap 89%). These results suggest that the molecular techniques used in this study could be useful for the phylogenetic analysis of Korean Artemisia herbs having variations in their morphological characteristics.

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Method and Reference Equipment for Evaluation of Travel Time Information (구간 통행시간정보 평가를 위한 기준장비 개발 및 평가 방법 연구)

  • Jeon, Hyeonmyeong;Cho, Yong-Sung;Ahn, Sun-Young;Lim, Sung Han
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.21 no.1
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    • pp.64-75
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    • 2022

  • The ITS performance evaluation has been performed in the evaluation of traffic data collection equipment. However, evaluation of the data collection equipment alone cannot guarantee the reliability of the traffic information. So, ITS service evaluation has to be implemented institutionally. In this study, an evaluation method has been prepared to evaluate the accuracy of travel time information in road sections. In addition, a piece of portable reference equipment was developed to collect travel time data on the road. Field tests were performed on two national road sections managed by the Seoul Construction and Management Administration (SCMA) to prepare an evaluation method considering field conditions and evaluate the reference equipment's performance. Based on the test results, the improvement of the reference equipment to collect more samples and the adjustment of collection points were discussed.

  • https://doi.org/10.12815/kits.2022.21.1.64 인용 PDF KSCI