• Journal of Microbiology and Biotechnology
• /
• 제16권5호
• /
• pp.804-807
• /
• 2006

Bacterial serine proteases, especially those from Bacillus, have been extensively studied. Intracellular serine protease 1 (Isp1) is responsible for most of the proteolytic activity in B. subtilis. To identify Isp1 substrates and study its physiological functions, a mutant of Isp1, which has lost the enzymatic activity, was constructed. Through a GST affinity chromatographic method, several Bacillus proteins that specifically interacted with S246A mutant Isp1 protein were isolated and then identified by MALDI-TOF analysis. ClpC and elongation factor Tu (EF-Tu) were among those proteins specifically bound to mutant Isp1. In addition, several proteins involved in stationary phase adaptive response (such as RNA polymerase sigma factor, spoIIIE) were also identified. These findings led us to suggest that the major function of this serine protease, whose expression is greatly increased during the stationary phase, is to mediate transition of the cell into the stationary phase in a proper and timely manner.

• 무역상무연구
• /
• 제14권
• /
• pp.257-287
• /
• 2000

Many problems and complaints have been caused by applying the UCP to the standby credit. To solve the problem, International Standby Practices were established. ISP and UCP are similar in that both of them generally regulate the transaction of credit. However, when the ISP is compared with the UCP, the following features are found : 1. In the UCP, when Force Majeure such as acts of God or strikes cause temporary work stoppage, the expiration date cannot be extended. In the ISP, the expiration date can be extended to 30 days afte the place for presentation re-opens for business in the same situation. 2. The UCP does not specify who the issuer of a document must be because there can be many issuers of documents. In the ISP, it is specified that all required documents are to be issued by the beneficiary. 3. In the UCP, compliance between presented documents is required. In the ISP, a discrepancy between presented documents is allowed. 4. In the UCP, if drawings and/or shipments are required by a credit to be made in instalments, and a required drawing/instalment is not made, the credit ceases to be available for any subsequent instalment. In ISP, there is no loss of effect and no influence on the right of beneficiaries, even in the same situation. 5. In the UCP, multiple transfers are not permitted, but partial transfers are. ISP states just the opposite. Multiple transfers are permitted, but partial transfers are not. 6 The UCP obligate each bank (issuer, confirming and nominated bank) to complete their review within a 'reasonable time' but not more than seven banking days. In the ISP, less than three business days is deemed to be not unreasonable and more than seven days is deemed to be unreasonable. 7. ISP, unlike UCP, recognizes that issuers and confirmers may spread their risk through syndication and participation of standby credits. However, the thing to remember is that the ISP should be reviewed carefully before application. If necessary, a partial addition or modifications can be made. Usually, the best advantage of the ISP is given to the issuers. A positive use of the ISP can be made by issuers but, applicants should consider using the UCP to the their rights and duties.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 2001년도 춘계학술발표회요약집
• /
• pp.201-201
• /
• 2001

• Journal of Microbiology and Biotechnology
• /
• 제5권3호
• /
• pp.149-153
• /
• 1995

A simple and low-cost medium was developed for the growth of Bifidobacterium longum KFRI 977. Of three bifidobacterial strains, B. longum KFRI 977 (ATCC 15707) showed the best growth in MRSC broth containing 0.3% oxgall, grew well in partially anaerobic condition, exhibited highest $\beta$-galactosidase activity, and was inhibitory against Clostridium perfringens KFRI 434. Of three developed media, the population of B. longum KFRI 977 was highest (1.9$\times$$10^9$/ml) in ISP based medium. The composition of ISP based medium is ISP (5%), glucose (1%), L-cysteine HCI (0.05%), Trypticase peptone (0.5%), yeast extract (0.5%), $MgSO_4$ (0.05%), Tween-80 (0.1%), and phosphate buffer (pH 7.0). Hydrolysis of ISP by Protease A was unnecessary, and the use of phosphate buffer (pH 7.0) prevented the formation of protein precipitate. Associative culture of B. longum KFRI 977 with Lactobacillus acidophilus KFRI 233 was proven to be deleterous to the growth of B. longum KFRI 977.

• Preventive Nutrition and Food Science
• /
• 제7권4호
• /
• pp.367-372
• /
• 2002

This study investigated the effects of dietary soy protein and Ca on the lipid profiles of rats fed fat-enriched diets. Rats were divided into two groups and fed either a casein-low Ca (Exp I) or ISP-high Ca diet (Exp II) for a control period of 4 weeks. The two groups of rats were again subdivided into 4 groups and fed one of 4 experimental diets for another 4 weeks. The experimental diets consisted of 18% beef tallow and 1 % cholesterol, in which either 20 % casein or ISP with one of two levels of Ca, high (1 %) or low (0.1 %). The concentrations of total lipid, cholesterol and triglyceride in serum, liver and feces were determined. At the end of the control period, the serum total lipid and cholesterol concentrations were low in the rats fed ISP-high Ca diet (67~76% and 83~86%). During the next 4 week period, these concentrations remained significantly lower in rats fed the diets containing ISP and high Ca compared with those on casein and low Ca diets (p ＜ 0.05). Total lipid and cholesterol concentrations in feces were significantly higher in the ISP-high Ca dietary group at 4 weeks, and high in both high Ca groups at 8 weeks. This study demonstrates that both soy protein and Ca reduce serum and liver cholesterol, triglyceride, and total lipid in rats fed fat-enriched diet, and that they have an additive effect when combined.

• 전기전자학회논문지
• /
• 제20권1호
• /
• pp.89-93
• /
• 2016

본 논문에서는 다양한 영상 전처리 알고리즘들을 적용할 수 있고, 영상 인식과 같이 ISP 응용 프로그램을 병렬로 처리 가능한 SIMT(Single Instruction Multiple Threads) 기반 Image Signal Processor를 제안하였다. 기존의 ISP는 입력 영상의 품질 개선을 위하여 영상 개선 알고리즘이 하드웨어로 설계되어 처리 속도는 빠르지만 다양한 영상 처리 알고리즘에 따라 성능 최적화에 어려움이 있었다. 제안한 ISP는 명령어를 기반으로 한 프로세서로서 다양한 영상 처리 알고리즘을 수행하고 SIMT 구조를 적용하여 알고리즘을 병렬로 수행해 성능을 개선하였다. 제안하는 ISP를 검증하기 위해 Xilinx Virtex-7을 탑재한 VC707 Board를 사용하였으며 cell multicore processor와 비교했을 경우 수행시간이 약 71%, ARM Cortex-A9과 ARM Cortex-A15와 비교하였을 경우 각각 63%, 33% 성능을 개선하였다.

• 한국전자거래학회:학술대회논문집
• /
• 한국전자거래학회 1999년도 학술대회지 vol.1
• /
• pp.215-231
• /
• 1999

CALS, Enterprise Integration, Virtual Enterprise 등을 구현하는데 있어서 BPR (business process re-engineering), ISP (Information Strategy Planning), Analysis/Design 등은 필수 적이다. 이러한 BPR, ISP, A/D 추진하는데 있어서 각 단계의 산출물은 유연하게 연계되지 못한다. 그 이유는 각 단계가 접근하는 초점이 다르고, 산출물의 성격이 다르며, 무엇보다도 각 단계의 전문가가 다르기 때문에 발생한다. 현재 기업이 각 단계를 추진할 때에는 반복적인 피드백을 통하여 정보를 공유하거나, 각 단계의 정보 연계가 제대로 이루어지지 않는다. 이러한 문제를 해결하기 위하여 BPR-ISP-A/D의 정보 연계를 유연하게 하기 위하여 PPO (Product-Process-Organization) 객체 진화에 의한 통합 방법론을 제안 하고자 한다. 그 과정은 1) Real world 정보를 PPO 객체로 정의하고, 2) PPO 객체를 기반으로 BPR을 수행하여 TO-BE model 을 만드는 동시에, 3) PPO TO-BE model과 함께 IT Architecture를 정의하며, 4) PPO 객체를 기반으로 Analysis and Design 과정을 통하여 Application system 객체 및 Data 객체를 정의한다. 이때 PPO 객체는 Real-world 객체에서 Application system 객체 및 Data 객체로 진화하게 되고 BPR-ISP-A/D의 seamless 통합이 이루어진다. 여기서, 단계 1) 과 2)는 System Engineering을 통하여 이루어진다. ARIS(Architecture of Integrated Information System, Dr. A-W. Scheer, 1998)개념을 도입하여 실세계를 정해진 규약에 따라 Model로 Mapping하고, 생성된 Model을 바탕으로 BPR을 수행하여 개선된 Model을 산출해 낸다. 단계 3)은, IE (Information Engineering, James Martine, 1990) 의 ISP가 도입되어, 설계업무를 지원하기위한 기본적인 시스템 구조를 구상하게 된다. 이와 함께 IT Model을 구성하게 되는데, 객체지향적 접근 방법으로 Model을 생성하고 UML(Unified Modeling Language)을 Tool로 사용한다. 단계 4)는 Software Engineering 관점으로 접근한다. 이는 최종산물이라고 볼 수 있는 설계업무 지원 시스템을 Design하는 과정으로, 시스템에 사용될 데이터를 Design하는 과정과, 데이터를 기반으로 한 기능을 Design하는 과정으로 나눈다. 이를 통해 생성된 Model에 따라 최종적으로 Coding을 통하여 실제 시스템을 구축하게 된다.

• 한국정보통신학회논문지
• /
• 제26권1호
• /
• pp.153-161
• /
• 2022

본 연구는 콘텐츠 제공자를 포함한 플랫폼 사업자가 광대역 인터넷 접속을 통해 고품질의 콘텐츠를 제공하는 경우 보편적 서비스 기여금을 분담할 때 이것의 경제적 효과를 분석한다. 주요 결과는 다음과 같다. 첫째, 플랫폼 사업자가 독점적 위치에 있다면 트래픽 이용량은 사회적 최적 수준보다 적으며, ISP가 결정하는 가격들은 콘텐츠 수요의 가격민감도에 영향을 받는다. 둘째, 플랫폼 사업자가 경쟁적인 경우 ISP가 책정하는 요금은 사회적 최적 수준과 동일하다. 셋째, 플랫폼 사업자가 콘텐츠 요금을 부과하지 않는 경우는 ISP는 요금을 사회적 최적 수준으로 결정하고 광고 수입이 많을수록 소비자에게 부과하는 망 이용대가는 줄어든다. 이는 플랫폼 사업자에게 고품질 콘텐츠 서비스를 제공할 수 있는 네트워크 서비스를 이용하는 대신, 보편적 서비스 기여분 분담을 의무화한다면 사회후생은 증가될 수 있기 때문에 플랫폼 사업자에게 보편적 서비스 기여분을 분담하는 것이 사회적으로 바람직하다는 것을 시사해 준다.

• 한국미생물·생명공학회지
• /
• 제46권2호
• /
• pp.114-119
• /
• 2018

Astaxanthin is one of the major carotenoids used in pigment has a great economical value in pharmaceutical markets, feeding, nutraceutical and food industries. This study was to increase the production of astaxanthin by co-expression with transformed Escherichia coli using six genes involved in the non-mevalonate pathway. Involved in the non-mevalonate biosynthetic pathway of the strain Kocuria gwangalliensis were cloned dxs, ispC, ispD, ispE, ispF, ispG, ispH and idi genes in order to increase astaxanthin production from the transformed E. coli. And co-expression with the genes to compared the amount of astaxanthin production. This engineered E. coli, containing both the non-mevalonate pathway gene and the astaxanthin biosynthesis gene cluster, produced astaxanthin at $1,100{\mu}g/g$ DCW (dry cell weight), resulting in approximately three times the production of astaxanthin.

• 무역상무연구
• /
• 제13권
• /
• pp.657-677
• /
• 2000

Since January 1, 1999, traders, bankers and their counsels worldwide have available for their use the first set of rules exclusively dealing with standby letters of credit:the International Standby Practices(ISP98). Numerous standbys have alreadby been issued in the United States and worldwide subject to the new ISP. The international banking community is anticipating an increasing demand from their customers to issue ISP-governed undertakings. Before the adoption of ISP, traders and bankers had only the choice of issuing their standby subject to the International Chamber of Commerce's(ICC) Uniform Customs and Practices for Documentary Credits(UCP) and, to a much lesser extent, to the ICC's Uniform Rules for Demand Guarantees(URDG). However, practice showed that UCP rules are not easily adapted to regulate transnational standbys. Indeed, UCP was conceived to govern documentary credits, which are intended to serve as a means of payment. By contrast, standbys are means of guaranty. The core of UCP cannot therefore be appropriate for standby practices and, as a consequence, a number of UCP's provisions have to be excluded in the standby's text. UCP's shortcomings indicated above fulfil the requirements of a key factor for the success of uniform rules. Indeed, to achieve success in the sense of meeting the market's acceptance, any such rules should fill a widely recognized need expressed by merchant community to which such rules are addressed. The ISP cleary has such a vocation. Nonetheless, the already largely encumbered regulatory environment of guarantee devices can hardly go unnoticed. The question therefore arises as to the proper place of ISP in such a context.