• Title/Summary/Keyword: Isotonic

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DILUTION AND SHORT-TERM STORAGE OF COCK SPERMATOZOA BY INHIBITION OF MOTILITY WITH FRUCTOSE AT AMBIENT TEMPERATURE

  • Mohan, J.;Moudgal, R.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.6
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    • pp.705-709
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    • 1996
  • A simplified dilutor for cock spermatozoa at ambient temperature was achieved by adjusting the 5% concentration of fructose in isotonic saline. Motility of cock spermatozoa was arrested completely for maximum 6 hrs without affection the survivability of spermatozoa by employing this sugar. To study the effect of high concentration of fructose on fertility, sperm were inseminated with or without fructose at different hrs. Fructose from semen samples was removed by centrifugation. High fertility obtained in the hens inseminated with fructose free sperm (washed). In addition, washed sperm maintained the 85.00% fertility for 6 hrs in winter season ($17-21^{\circ}C$) and 82.67% fertility for 3 hrs in summer season ($31-35^{\circ}C$). Whereas control groups showed 47.33 and 25.33% fertility in winter and summer season respectively. No significant difference was found in percent motility and live counts between the control and washed experimental groups during winter season. However, these measures differed significantly in summer. Washing of cock spermatozoa more than once, high speed centrifugation and more duration for centrifugation proved harmful to fertility. It may be concluded that fructose (5%) can be used as a motility or metabolic inhibitor of spermatozoa for short-term storage of cock semen at ambient temperatures.

The Effects of Proprioceptive Neuromuscular Facilitation Techniques on the Quadriceps Femoris by Electromyographic Analysis (고유수용성 신경근 촉진기술에 따른 대퇴사두근의 활동전위)

  • Sin, Eun-Sung;Choi, So-Young
    • Physical Therapy Korea
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    • v.4 no.2
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    • pp.66-76
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    • 1997
  • The purpose of this study was to compare the integrated electromyographic activity ratios of vastus lateralis(VL); rectus femoris lateral portion (RFL); rectus femoris medial portion(RFM); and vastus medialis(VM) muscles of 30 healthy subjects under three proprioceptive neuromuscular facilitation(PNF) techniques. Each subject was randomly assigned to one of 3 PNF techniques groups : slow reversal(SR), slow reversal hold(SRH) and rhythmic stabilization (RS). Each person was positioned in supine with the right hip flexed to $45^{\circ}$ and the knee fully extended and received a total of 6 sessions. Each technique was applied to the right lower extremity in two diagonal patterns while electrical activity was monitored from the ipsilateral muscles VL, RFL, RFM, and VM, respectively. Comparison of normalized mean EMG magnitudes from VL, VM showed that RS demonstrated significantly greater activity than that of SR or SRH and that RFL and RFM did not demonstrate any greater relative EMG activity with the three PNF techniques than did VL or VM.

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Effects of Oxidative Stress Induced by Diquat on Arginine Metabolism of Postweaning Pigs

  • Zheng, Ping;Yu, Bing;Lv, Mei;Chen, Daiwen
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.1
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    • pp.98-105
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    • 2010
  • A total of 16 crossbred post-weaning pigs (10.64${\pm}$0.27 kg BW) were individually penned and assigned to one of two treatments to investigate the influences of diquat-induced oxidative stress on performance and arginine metabolism. Pigs in the oxidative stress group were injected intra-peritoneally with 10 mg/kg BW of diquat, while the control group were injected with isotonic saline. All pigs were fed ad libitum. The experiment lasted for 7 days. The results indicated that compared with control treatment, oxidative stress induced by diquat significantly decreased average daily gain, intake and feed conversion. The treatment decreased activities of antioxidant enzymes, increased concentration of malondialdehyde in plasma, increased cationic amino acid transporter-1 mRNA level and activity of ornithine aminotransferase and concentrations of arginine and citrulline in the jejunum, decreased the concentrations of arginine in plasma and kidney, and decreased induced nitric oxide synthase mRNA level. It is concluded that oxidative stress induced by diquat can influence absorption and metabolism of arginine and consequently modify the requirement of arginine for post-weaning pigs.

Effect of Various Factors on the Operational Stability of Immobilized Cells for Acrylamide Production in a Packed Bed Reactor

  • Lee, Cheo-Young;Choi, Sang-Kyo;Chang, Ho-Nam
    • Journal of Microbiology and Biotechnology
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    • v.3 no.1
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    • pp.39-45
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    • 1993
  • The effect of concentrations of phosphate buffer and acrylonitrile, pH, and various salts on the operational stability of the immobilized cells of Brevibacterium CH2 in a packed bed reactor were investigated. The effects of salts and carriers on the swelling of the immobilized beads during hydrolysis in a columnreactor were also investigated. Immobilization of the cells in Ba-alginate was more desirable than those in polyacrylamide and Ca-alinate for the swelling of the immobilized beads and the desired quality of the acrylamide produced. High quality acrylamide was produced using the Ba-alginate beads in a recycle fed-batch reactor without using an isotonic substrate. The conversion yield was nearly 100%, including a trace amount of acrylic acid produced as a by-product.

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Gait Training Strategy by CPG in PNF with Brain Injured Patients (고유수용성 신경촉진법에서 CPG를 이용한 뇌손상자 보행훈련전략)

  • Bae Sung-soo
    • The Journal of Korean Physical Therapy
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    • v.17 no.1
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    • pp.108-122
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    • 2005
  • The gait training strategy in very important things for central nervous system(CNS) injury patients. There are many method and strategy for regaining of the gait who had CNS injury. A human being has central pattern generator(CPG) is spinal CPG for locomotion. It is a neural network which make the cyclical patterns and rhythmical activities for walking. Sensory input from loading and hip position is essential for CPG stimulation that makes the central neural rhythm and pattern generating structure. From sensory input, the proprioceptive information facilitate proximal muscles that controlled in voluntarily from cortical level and visual and / or acoustical information facilitate distal muscles that controlled voluntarily from subcortical level. Gait training method can classify that is functional level and structural level. Functional level includ level surface gait, going up and down the stair. It is important to facilitate a guide tempo in order to activate the central pattern generators. During the functional test or functional activities, can point out the poor period in gait that have to be facilitate in structural level. There are many access methods with patient position and potentiality. The methods are using of rhythmic initiation, replication and combination of isotonic with standing position. Clinically using it on weight transfer onto the stance leg, loading response, loading response and pre-swing, terminal stance, up and downwards stairs.

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A Case Report of PNF Strategy Applied ICF Tool on Upper Extremity Function for Patient Adhesive Capsulitis (유착성 관절낭염 환자의 상지 기능에 대한 ICF Tool을 적용한 PNF 중재전략의 증례보고)

  • Kang, Tae-Woo;Kim, Tae-Yoon
    • Journal of the Korean Society of Physical Medicine
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    • v.12 no.4
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    • pp.19-28
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    • 2017
  • PURPOSE: The purpose of this study was to describe the Proprioceptive Neuromuscular Facilitation (PNF) Intervention strategy applied International Classification of Functioning, Disability and Health (ICF) Tool about strength, range of motion, scapular stability, pain and function of shoulder for patients with adhesive capsulitis. METHODS: The data was collected by patient with adhesive capsulitis. The patient was a 50-year-old male diagnosed with right shoulder with adhesive capsulitis. We applied the PNF Intervention strategy applied ICF Tool to patient with adhesive capsulitis. PNF interventions were consisting of such as combination of isotonic and stabilizing reversal technique and various positions. PNF interventions were applied, such as those aiming at decreasing pain and disability and increasing range of motion and function for the four weeks. Parameters of result were collected for strength, range of motion, scapular stability, pain and function of shoulder using the hand held dynamometer, goniometer, lateral scapula slide test, and shoulder pain and disability index, respectively. RESULTS: Clinical benefits were observed the patient with adhesive capsulitis for strength, range of motion, scapular stability, pain, and function of shoulder. The patient with adhesive capsulitis improved strength, range of motion, scapular stability, pain, and function of shoulder. CONCLUSION: Patient reported improved strength, range of motion, scapular stability, pain, and function of shoulder after intervention.

Studies on Drug Absorption Characteristics for Development of Ocular Dosage Forms: Ocular and Systemic Absorption of Topically Applied ${\beta}-Blockers$ in the Pigmented Rabbit

  • Lee, Yong-Hee
    • Journal of Pharmaceutical Investigation
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    • v.24 no.3
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    • pp.59-66
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    • 1994
  • The objective of this study was to determine the influence of drug lipophilicity on the extent of ocular and systemic absorption following topical solution instillation in the pigmented rabbit. ${\beta}-Blockers$ of various lipophilicity were chosen as model drugs, $25\;{\mu}l$ of a 15 mM drug solution in isotonic pH 7.4 buffer was instilled, and ocular tissue and plasma drug concentrations were monitored. Ocular absorption was apparently increased in all eye tissues, but non-corneal absorption ratio was decreased by increasing of drug lipophilicity. Systemic bioavailability was ranged from 61% for atenolol to 100% for timolol, and at least 50% of the systemically absorbed drug reached the blood stream from the nasal mucosa. Occluding the nasolacrimal duct for 5 min reduced the extent of systemic absorption of timolol and levobunolol, but did not do so for atenolol and betaxolol. Taken together, the ocular absorption of topically applied ophthalmic drugs would be modest for lipophilic drugs. By contrast, the systemic bioavailability would be modest for drugs at the extremes of lipophilicity, and the nasal contribution to systemically absorbed drug diminished with increasing of drug lipophilicity.

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Effect of Ginseng Saponin, Gypsophila Saponin, and Detergents on Volume Changes and Fragility of Red Blood Cells (인삼(人蔘) Saponin, 은시호(銀柴胡) Saponin 및 계면활성제(界面活性劑)가 적혈구(赤血球)의 용적변화(容積變化) 및 Fragility에 미치는 영향(影響))

  • Lee, Shin-Woong;Lee, Jeung-Soo;Lee, Soo-Kun;Ok, Chang-Kun;Kim, Young-Hie
    • YAKHAK HOEJI
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    • v.33 no.1
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    • pp.15-19
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    • 1989
  • The effects of Gypsophila saponin, sodium dodecylsulfate (SDS) and Triton X-100 on volume changes and fragility of red blood cells were compared to ginseng saponin to elucidate whether there are any difference in their action on membrane lipid. Cell volume was decreased to about 38% in 1M NaCl and increased to about 20% in 1/10M NaCl. Hematocrit value was decreased by Gypsophila saponin, SDS, and Triton X-100 which caused hemolysis in isotonic NaCl solution. These detergents also inhibited increase of cell volume and accelerated hemolysis in hypotonic solution. However, ginseng saponin did not influence to osmotic volume changes and hemolysis of red blood cells. These results suggest that the disruptive effect of plant saponin on membrane barriers induced by removing membrane lipid is different from their source and ginseng saponin has very low affinity to membrane lipid.

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Back strength and relevance of CPR chest compression (배근력과 심폐소생술의 가슴압박과의 관련성)

  • Choi, Sung-Soo;Han, Mi-Ah;Yun, Seong-Woo;Ryu, So-Yeon
    • The Korean Journal of Emergency Medical Services
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    • v.17 no.2
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    • pp.57-64
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    • 2013
  • Purpose : The purpose of the study is to investigate the quality and relevance of back strength or chest compression which is applied by isotonic exercise of hip joint. Methods : Subjects were 37 students who participated in the BLS course and accepted the informed consent from December 7 to 8, 2012. During CPR performance, back strength was measured by the researcher. CPR was used the manikin for practical training with using PC, conducted by standard CPR for 2 minutes, Quality of chest compressions included average chest compression depth, rate, and recoil ratio. Results : Back strength (kg) is related to the chest compression depth (mm) (r =.746, p <.001). The high quality CPR is the most important factor so high quality is full chest recoil of chest compression and chest compression depth (mm) (${\beta}$=.831, p <.001). In this study, chest compression rate and recoil ration were not influenced by back muscle strength. Conclusion : It is necessary to implement the CPR program to improve physical strength and effective performance of CPR.

Noise Reduction Methods for the EMG Median Frequency Data in Fatiguing Isotonic Exercise (등장성 운동 시 근전도 중앙주파수 데이터의 잡음 제거 방법)

  • Cho, Sang-Hyun
    • Physical Therapy Korea
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    • v.8 no.4
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    • pp.31-43
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    • 2001
  • 19명의 건강한 성인 남자의 우세팔쪽 위팔두갈래근에서 피로가 생길 때까지 2.4초를 하나의 주기로 팔꿉을 반복적 등장성으로 굽히고 펴서 표면근전도 신호를 얻었다. 처리과정 A 중앙주파수(MDF )는 이 신호의 0.5초 구간을 power spectrum analy sis (PSA)로 계산하였는데 상당량의 잡음이 있었다. 중앙주파수의 잡음 양을 비교하기 위해, 동일한 표면근전도에서 3번까지 신호를 받았다 (2.4초 구간을 PSA로 계산한 처리과정 B, 13 point 로 moving averages한 처리과정 C, digital low pass filter한 처리과정 D). 그리고 나서 그 신호의 주요 주파수 성분을 뽑아내었다. 위의 중앙주파수 자료와 시간간의 회귀직선을 분석하면 초기 중앙주파수, 회귀기울기, 그리고 피로지수와 같은 모수를 얻을 수 있다. 비모수 검정의 하나인 Kendall 기법으로 네 개의 처리과정간의 모수를 비교하였다. 통계결과 잡음이 처리과정 A보다 B, C, D에서 적었고, D에서 가장 적게 나타났다. 중앙주파수를 digital low pass로 여과(filtering)함으로써 앞으로 있게 될 동적 운동 시 근피로 모니터기의 신뢰도를 높일 수 있다.

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