Effect of Ginseng Saponin, Gypsophila Saponin, and Detergents on Volume Changes and Fragility of Red Blood Cells

인삼(人蔘) Saponin, 은시호(銀柴胡) Saponin 및 계면활성제(界面活性劑)가 적혈구(赤血球)의 용적변화(容積變化) 및 Fragility에 미치는 영향(影響)

The effects of Gypsophila saponin, sodium dodecylsulfate (SDS) and Triton X-100 on volume changes and fragility of red blood cells were compared to ginseng saponin to elucidate whether there are any difference in their action on membrane lipid. Cell volume was decreased to about 38% in 1M NaCl and increased to about 20% in 1/10M NaCl. Hematocrit value was decreased by Gypsophila saponin, SDS, and Triton X-100 which caused hemolysis in isotonic NaCl solution. These detergents also inhibited increase of cell volume and accelerated hemolysis in hypotonic solution. However, ginseng saponin did not influence to osmotic volume changes and hemolysis of red blood cells. These results suggest that the disruptive effect of plant saponin on membrane barriers induced by removing membrane lipid is different from their source and ginseng saponin has very low affinity to membrane lipid.