• Title/Summary/Keyword: Isolation unit

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Acclimation of magnetic activated sludge with 1,4-dioxane and analysis of bacterial flora in the sludge

  • Toshiyuki Nikata;Hayato Ogihara;Yasuzo Sakai
    • Progress in Superconductivity and Cryogenics
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    • v.25 no.3
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    • pp.7-12
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    • 2023
  • Isolation of pollutant-degrading bacteria is important in bioaugmentation, one of the methods for biological degradation of environmental contaminants. We focused on the magnetic activated sludge (MAS) process as a culture method that efficiently concentrates degrading bacteria, and cultured activated sludge with 1,4-dioxane as a model pollutant. After 860 days of operation, MLVSS, which indicates the amount of sludge, increased from 390 mg/L to 10,000 mg/L, and the removal rate of organic matter including 1,4-dioxane, tetrahydrofuran, and glucose in the artificial wastewater reached up to 97%. Based on these results, the MAS process was successfully used to acclimate activated sludge with 1,4-dioxane. Bacterial flora analysis in the MAS showed that bacteria of the genus Pseudonocardia, already reported as 1,4-dioxane degrading bacteria, play an important role in the degradation of this pollutant. The MAS process is a suitable culture method for acclimation of environmental pollutants, and the findings indicate that it can be used as an enrichment unit for pollutant-degrading bacteria.

Isolation, Identification, and Fermentation Characteristics of Bacillus sp. with High Protease Activity from Traditional Cheonggukjang (전통 청국장으로부터 protease 분비능이 우수한 Bacillus sp. 균주의 분리 동정 및 발효 특성)

  • Ahn, Yong-Sun;Kim, Yong-Suk;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.38 no.1
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    • pp.82-87
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    • 2006
  • Twenty one strains strongly producing protease were isolated from Korean traditional Cheonggukjang. Eight strains selected by sensory evaluation on Cheonggukjang prepared with isolated strains were identified with based on biochemical properties a and 16S rDNA sequencing. Identified strains were Bacillus subtilis MB4, and Bacillus amyloliquefaciens A1, A2, B1, MC1, SB2, SC1, and SD1. Protease activities, important strain selection factor, were higher in Cheongjukjang prepared with B. subtilis MB4 (179.6 Unit) and B. amyloliquefaciens SB2 (201.9 Unit) than commercial traditional Cheonggukjang (97.9 Unit). Sensory evaluation revealed Cheonggukjang prepared with B. subtilis MB4 had flavor very similar to commercial traditional Cheonggukjang. Cheonggukjang prepared with B. suhtilis MB4 (0.0006 Pa s) and commercial traditional Cheonggukjang (0.0002 Pa s) revealed lower viscosities than those of Cheonggukjang prepared with B. amyloliquefaciens SB2, MC1, B1, A1, SD1, A2, and SC1 (0.006 to 0.008 Pa s at 1001/s. Results show Cheonggukjang could be prepared using single strain of B. subtilis MB4, maintaining high protease activity and very similar sensory and viscosity qualities with those of commercial traditional Cheonggukjang.

A Study on the Design and Implementation of SHF band Antenna for Digital Satellite Communication (디지털위성중계기용 SHF 대역 안테나 설계 및 구현에 대한 연구)

  • Kim, Ki-Jung;Han, Jun-Yong
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.11 no.1
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    • pp.12-17
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    • 2018
  • This study describes the design and implementation of Antenna for Digital Satellite Communication. The Antenna unit for SHF band consists of Reflector, Septom Polarizer, Feed Horn and Support Frame etc. Thought analysis of space environment before production, the possibility of the malfunction of equipment minimized and we designed a reliable Antenna through simulation for vibration and thermal analysis generated during the launch, and compared pre-simulation of main performance results to test results about main performances of Antenna after production. After fabricating the antenna, the maximum gain of the antenna main beam is 36.5dBi, which satisfies the requirement of 35dBi or more, and it also satisfies the requirement of -20dB for return loss of less than -24dB. Also, the isolation of the transmission and reception of the antenna is -22.6dB or less, which satisfies the requirement of -20dB or less. The antenna for digital satellite communication described in this paper can be used in the satellite field of geostationary earth orbit and low earth orbit requiring high reliability in the future.

Dual-Wideband Bandpass Filter Using Distributed Composite Right/Left-Handed Transmission Line Quad-Mode Resonators (분산 CRLH 전송선로 4중 모드 공진기를 이용한 이중-광대역 대역통과 여파기 설계)

  • Sung, Gyuje;Kim, Young
    • Journal of Advanced Navigation Technology
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    • v.21 no.1
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    • pp.84-89
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    • 2017
  • This paper presents a dual-wideband bandpass filter (BPF) with high band-to-band isolation and skirt selectivity using distributed composite right/left-handed (CRLH) transmission line (TL) quad-mode resonators (QMRs). The results of the proposed distributed CRLH TL unit cell analysis are used to establish the scattering parameters and the resonance frequencies of the QMR constituting the dual-wideband BPF. A novel dual-wideband bandpass filter is designed and fabricated, using the derived scattering characteristics. The measured results show that the fabricated dual-wideband bandpass filter has an insertion loss of less than 1.08dB in the lower band, and of 2.01dB in the upper band, a bandwidth of 2.8-5.52GHz and 9.68-12.26GHz, and a band-to-band isolation of more than 38dB, from 6.34-8.42GHz.

SPST Switch MMIC for Microwave Switch Matrix (마이크로웨이브 스위치 메트릭스 용 SPST 스위치 MMIC)

  • Chang Dong-Pil;Yom In-Bok;Oh Seung-Hyueb
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.2A
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    • pp.201-206
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    • 2006
  • A SPST Switch MMIC which used for Microwave Switch Matrix(MSM) of communications satellite payload with multi-beam function has been designed and fabricated. New RE FET switch configuration has been devised to improve power characteristics and isolation. Input and output return losses are better than another switches reported previously for both On and Off states. The MMIC chips were fabricated in 0.15um GaAs pHEMT process and measured insertion loss less than 2.0dB and isolation more than 63dB in the frequency range of 3GHz$\∼$4GHz. Output 3rd order interceptpoint above 32dBm has been recorded and the value is very high even though the unit pHEMT has gate width of 0.2mm and only four pHEMT are used in the MMTC.

Optimization of Nitrogen and Phosphorus Removal of Temporal and Spatial Isolation Process by Model Simulation System (시공간 동시분할 공정 시뮬레이션을 통한 질소 및 인 제거 최적화 방안)

  • Ryu, Dongjin;Chang, Duk;Shin, Hyungsoo;Park, Sangmin;Hong, Kiho;Kim, Sooyoung;Kim, Myoungjun
    • Journal of Korean Society on Water Environment
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    • v.23 no.2
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    • pp.206-215
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    • 2007
  • The objective of this study was to establish the optimal system operating strategies for nitrogen and phosphorus removal through model simulation system built for advanced wastewater treatment targeting on simultaneous temporal/special phase isolation BNR process. The simulation system was built with unit process modules using object modules in GPS-X code. The system was well verified by field experiment data. Simulation study was carried out to investigate performance response to design and operation parameters, i.e. hydraulic retention time (HRT), solids retention time (SRT), and cycle time. The process operated at HRTs of 10~15 hours, longer SRTs, and cycle time of 2 hours showed optimal removal of nitrogen. The HRTs of 10~15 hours, SRTs of 20~25 days, and longer cycle time was optimal for phosphorus removal. Both simulation and field studies showed that optimal operating strategies satisfying both the best nitrogen and phosphorus removals include HRTs ranged 10~15 hours, SRTs ranged 20~25 days, and cycle times of 4~8 hours. The simulation system with modularization of generalized components in BNR processes was, therefore, believed to be a powerful tool for establishing optimal strategies of advanced wastewater treatment.

Shaking table tests of prestressed damping-isolation units using a spring and rubbers

  • Yang, Keun-Hyeok;Mun, Ju-Hyun;Im, Chae-Rim;Won, Eun-Bee
    • Earthquakes and Structures
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    • v.23 no.4
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    • pp.373-384
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    • 2022
  • To improve the seismic performance of suspended ceiling structures, various vibration-damping devices have been developed. However, the devices made of metals have a limit in that they cause large deformation and seriously damages the exterior of the suspended ceiling structure from the wall. As a results, their strengthening effect of the suspended ceiling structure was minimal. Thus, this study employed a spring and vibration-proof rubber effectively controlled vibrations without increasing horizontal seismic loads on the ceiling to enhance the seismic resistance of suspended ceiling structures. The objective of the study is to examine the dynamic properties of a seismic damping-isolation unit (SDI) with various details developed. The developed SDI was composed of a spring, embossed rubbers, and prestressed bolts, which were the main factors enhancing the damping effect. The shaking table tests were performed on eight SDI specimens produced with the number of layers of embossed rubber (ns), presence or absence of a spring, prestressed force magnitude introduced in bolts (fps), and mass weight (Wm) as the main parameters. To identify the enhancement effect of the SDI, the dynamic properties of the control specimen with a conventional hanger bolt were compared to those of the SDI specimens. The SDI specimens were effective in reducing the maximum acceleration (Ac max), acceleration amplification factor (αp), relative displacement (δR), and increasing the damping ratio (ξ) when compared to the control specimen. The Ac max, αp, and δR of the SDI specimens with two rubbers, spring, and fps of 0.1fby, where fby is the yielding strength of the screw bolt were 57.8%, 58.0%, and 61.9% lower than those of the conventional hanger bolt specimens, respectively, resulting in the highest ξ (=0.127). In addition, the αp of the SDI specimens was 50.8% lower than those specified in ASCE 7 and FEMA 356. Consequently, to accurately estimate the αp of the SDI specimens, a simple model was proposed based on the functions of fps, stiffness constant of the spring (K), Wm, and ns.

Isolation of Pseudoxanthomonas sp. W12 and WD32 Producing Extracellular Protease (단백질분해효소를 생산하는 Pseudoxanthomonas sp. WD12와 WD32의 분리)

  • Cho, Woon-Dong;Lee, Je-Kwan;Lim, Chae-Sung;Park, A-Rum;Oh, Yong-Sik;Roh, Dong-Hyun
    • Korean Journal of Microbiology
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    • v.46 no.1
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    • pp.63-67
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    • 2010
  • Proteases catalyze hydrolytic cleavage of a peptide bond between amino acids and occupy pivotal positions in application in physiological and commercial fields. During the screening for novel bacteria producing extracellular protease, two bacterial strains, WD12 and WD32, were isolated from rotten trees and they made clear zone on LB plates supplemented with 1% skim milk. The similarities of 16S rRNA gene sequence of either WD12 or WD32 to GenBank database showed the highest to Pseuoxanthomonas mexicana as 97.8 and 99.8%, respectively. Phylogenetic analysis showed that both isolated was located within the cluster comprising P. mexicana and P. japonesis. WD12 and WD32 were catalase- and oxidase-positive, Gram-negative rod strains. In case of WD12, it could assimilate malate, but could not assimilate D-mannose, which were different characteristics from P. mexicana. Both Pseuoxanthomonas sp. WD12 and WD32 optimally produced extracellular protease at $35-37^{\circ}C$, and maximal activity showed as 656 unit/ml and 267 unit/ml, respectively.

Development of Xenon Feed System for a Hall-Effect Thruster to Space-propulsion Applications (우주추진용 홀방식의 전기추력기를 위한 제논연료공급장치 개발)

  • Kim, Youn-Ho;Kang, Seong-Min;Jung, Yun-Hwang;Seon, Jong-Ho;Wee, Jung-Hyun;Yoon, Ho-Sung;Choe, Won-Ho;Lee, Jong-Sub;Seo, Mi-Hui
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.39 no.1
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    • pp.84-89
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    • 2011
  • A Xenon Feed System (XFS) has been developed for hall-effect thruster to small satellite space-propulsion system applications. The XFS delivers low pressure gas to the Anode and Cathode of thruster head unit from a xenon storage tank. Accurate throttling of the propellant mass flow rate is independently required for each channel of the thruster head unit. The mass flow rate to each channel is controlled using the accumulator tank pressure regulation through a micron orifice and isolation valve. This paper discusses the Xenon Feed System design including the component selections, performance estimation and functional test.

The Isolation of Bacillus sphaericus 366M-9 Producing New Cephalosporin-C Deacetylase (CAH) and its Enzymatic Characterization (신규 Cephalosporin-C Deacetylase(CAH) 생산 균주인 Bacillus sphaericus 366M-9의 선발 및 그 효소학적 특성)

  • 이승훈;권태종;이동희
    • Microbiology and Biotechnology Letters
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    • v.32 no.3
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    • pp.224-229
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    • 2004
  • Several microorganisms (esterase-producing group) were isolated by the solid selective media containing-naphtylacetate. Among them, strain 366M-9 having a high activity of cephalosporin-C deacetylase (CAH; EC 3.1.1.41) was selected. The strain 366M-9 was identified as Bacillus sphaericus on the basis of morphological, physiological, and biochemical characteristics. The production of CAH reached at maximum value after 32 hrs, when cultivated in the optimal medium containing dextrin 2.5%, peptone 2.5%, sodium chloride 0.5%, dipotassium phosphate 0.25%, ferrous sulfate 0.02%, and 7-ACA 0.1% at $30^{\circ}C$ with initial pH 6.0. The CAH was purified by 3 steps with ammonium sulfate precipitation, adsorption chromatography on hydroxyapatite column, and Sephadex G-200 gel chromatography. The final enzyme preparation was homogeneous as judged by the analysis of SDS-PAGE and HPLC. Optimum temperature and pH for CAH activity were $50{\circ}C$ and around 7.0, respectively. And the enzyme was stable at pH 6.0~8.0, up to $50^{\circ}C$. The Michaelis-Menten constants ($K_{m}$ ), $V_{max}$ were 0.87 mM and 1.22 unit/ml, respectively.