• Title/Summary/Keyword: Isolation plate

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Isolation and Characterization of Bacillus thuringiensis strain AF6 Producing an Antifungal Substance and a Mosquitocidal Delta-endotoxin Simultaneously (모기유충에 대한 살충성 내독소와 항진균성 물질을 동시에 생산하는 B. thuringiensis AF6균주의 분리 및 특성)

  • 김광현;이광배;신두만
    • Journal of environmental and Sanitary engineering
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    • v.13 no.2
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    • pp.40-46
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    • 1998
  • For a biological control on a plant pathogen, Pryicularia Oryzae, and a mosquito, Aedes aegypti, Bacillus thuringiensis strain AF6 which produces parasporal inclusion, delta-endotoxin, was isolated. The B. thuringiensis strain AF6 was produced not only an antifungal substance(AFS) against P. oryzae, but also a mosquitocidal delta-endotoxin. The AFS of the strain AF6 in more stable at pH 4.0 than pH 10.0. At the mode of action, the AFS of the strain AF6 was inhibited hypha growth on potato agar plate(pH 5.0), and degraded cell walls of P. oryzae.

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c-CMG Cluster for Small Satellites

  • Lee, Seung-Mok;Seo, Hyun-Ho;Rhee, Seung-Wu
    • International Journal of Aeronautical and Space Sciences
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    • v.8 no.1
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    • pp.105-114
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    • 2007
  • This article presents the details of a designed control moment gyroscope (CMG) with a constant speed momentum wheel and one-axis-gimbal, and its experimental results performed at Korea Aerospace Research Institute. The CMG which is able to produce a torque of lOO mNm per each, is mounted in a pyramid configuration with four SGCMGs. Each CMG test and a single axis maneuver test with four-CMG cluster configuration are performed to confirm their performance on a ground-test facilities consisted of three major parts: a vibration isolation system, a dynamic force plate (Kistler sensor), and a DSP board. These facilities provide the accurate data of three axial and torques from the control moment gyro. Details of the CMG experimental results are presented with discussion of the experimental errors. The experimental data are compared with theoretical results and both results are used to verify their performance specifications.

Isolation and Identification of Tetrodotoxin-producing Marine Microorganism from Pufferfish (복어로부터 복어독(Tetrodotoxin) 생성능이 있는 해양 미생물의 분리 및 동정)

  • 윤성준;차병윤;이명자;정동윤;송병권;김희숙;김동수;이은열
    • Journal of Life Science
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    • v.9 no.6
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    • pp.653-658
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    • 1999
  • A novel marine microorganism, Vibrio sp. YE-101, was isolated from pufferfish and investigated for its ability to synthesize tetrodotoxin (TTX). Various strains isolated from the intestine of pufferfish were grown on TCBS agar plate, and then cultured on Ocean Research Institute (ORI) medium supplemented with 3% NaCl at 23$^{\circ}C$ for 3days. The cells were harvested, disrupted, fractionated by Bio-Gel P-2 column chromatography and then TTX-producing strain, Vibrio sp. YE-101, was identified using mouse bioassay. The isolated TTX from Vibrio sp. YE-101 was also analyzed and identified by HPLC and gas chromatography-mass spectrometer (GC-MS). The mass fragmentation of trimethylsilyl derivatives of C9-base of TTX from Vibrio sp. YE-101 was interpreted and the pattern of fragmentation was same with that of authentic standard. The purfied TTX was also positive to the mouse bioassay, which clearly represents that Vibrio sp. YE-101 can synthesize TTX.

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Variation of Soil Mycoflora in Decomposition of Rice Stubble from Rice-wheat Cropping System

  • Vibha, Vibha;Sinha, Asha
    • Mycobiology
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    • v.35 no.4
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    • pp.191-195
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    • 2007
  • The colonization pattern and extent of decay produced in paddy stubble by soil inhabiting mycoflora were done by using nylon net bag technique. Among the three methods used for isolation of fungi, dilution plate technique recorded the highest number of fungi followed by damp chamber and direct observation method. Nutrient availability and climatic conditions (temperature, humidity and rainfall) influenced the occurrence and colonization pattern of fungi. Maximum fungal population was recorded in October ($48.99{\times}10^4/g$ dry litter) and minimum in May ($11.41{\times}10^4/g$ dry litter). Distribution of Deuteromycetous fungi was more in comparison to Zygomycetes, oomycetes and ascomycetes. In the early stage of decomposition Mucor racemosus, Rhizopus nigricans, Chaetomium globosum and Gliocladium species were found primarly whereas at later stages of decomposition preponderance of Aspergillus candidus, Torula graminis, Cladosporiun cladosporioides and Aspergillus luchuensis was recorded.

Low Pull-in Voltage MEMS Switches for Wireless Applications (저전압구동 무선통신용 MEMS 스위치)

  • Shim, Dong-Ha;Lee, Moon-Chul;Lee, Eun-Sung;Park, Sun-Hee;Kim, Young-Il;Song, In-Sang
    • Proceedings of the KIEE Conference
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    • 2002.07c
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    • pp.1969-1971
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    • 2002
  • This paper presents the design and performance of low pull-in voltage MEMS switches for commercial cellular/PCS applications. The switches have all-metal (3 ${\mu}m$ thick Au) movable plates over CPW(Coplanar Waveguide) transmission line. The stress gradient in a movable plate is considered in mechanical design to obtain an accurate pull-in voltage. Series metal-to-metal contact switches are fabricated and evaluated. Those switches exhibit the low loss(<0.2 dB @1.9 GHz) with good isolation(55 dB @1.9 GHz).

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Isolation of $\alpha$-1,3 Glucanase from Microorganism and the Prodution of High Activity $\alpha$-1,3 Glucanase for Hydrolysis of Dental Plaque (치면세균막 분해효소인 $\alpha$-1,3 glucanase를 생산하는 미생물의 분리 및 효소 특성)

  • 조효상;허태련;윤정원
    • Microbiology and Biotechnology Letters
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    • v.21 no.3
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    • pp.263-268
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    • 1993
  • Seventeen strains were isolated from soil, cattle rumen, cereal sewage dregs, insect on agar plate containing insoluble glucan as a sole carbon source from immobilized Streptococcus mutans, which produced alpha-1,3 glucanase for lysis of dental plaque. Among these strains isolated from soil, SW-522 and SW-713 that had appeared to produce the high level of alpha-1,3 glucanase, degraded insoluble glucan from S. mutans 97.6% and 49.4%, respectively in 5 hours. The activity of crude alpha-1,3 glucanase from SW-522 was 1.3mg insoluble glucan/min.mg protein. This enzyme was entirely degraded insoluble glucan on glass tube which produced by S. mutans in TH medium with 5% sucrose.

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Isolation of Myxobacteria from Soil and RFLP Analysis of 16S rDNA Fragments. (토양으로부터 Myxobacteria의 분리 및 165 rDNA RFLP분석)

  • 김수광;최병현;김종균;이병규;강희일
    • Korean Journal of Microbiology
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    • v.39 no.3
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    • pp.187-191
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    • 2003
  • In an attempt to isolate myxobacteria from soil samples, we isolated swarm and fruiting body forming bacteria that have bacteriolytic activity on Coli-spot agar plate. For the classification of myxobacteria, 16S rDNA RFLP patterns were analyzed. Amplified 16S rDNAs of myxobacteria type strains (Family I, II, III and IV), negative control strains and soil-isolates were restricted with HaeIII, EcoRI and EcoRV, respectively. We found that the soil-isolates belongs to myxobacteria Family I, II, III.

An Eight-Way Radial Switch Based on SIW Power Divider

  • Lee, Dong-Mook;An, Yong-Jun;Yook, Jong-Gwan
    • Journal of electromagnetic engineering and science
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    • v.12 no.3
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    • pp.216-222
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    • 2012
  • This paper presents a single-pole eight-throw switch, based on an eight-way power divider, using substrate integrate waveguide(SIW) technology. Eight sectorial-lines are formed by inserting radial slot-lines on the top plate of SIW power divider. Each sectorial-line can be controlled independently with high level of isolation. The switching is accomplished by altering the capacitance of the varactor on the line, which causes different input impedances to be seen at a central probe to each sectorial line. The proposed structure works as a switching circuit and an eight-way power divider depending on the bias condition. The change in resonant frequency and input impedance are estimated by adapting a tapered transmission line model. The detailed design, fabrication, and measurement are discussed.

Airborne Microorganisms of Some Underground Shops in Pusan (부산시 일부 지하상가 공기중 부유 균주)

  • 이용환;정석훈;고광욱;김영기;변주현
    • Journal of Life Science
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    • v.10 no.1
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    • pp.74-78
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    • 2000
  • This study was investigated to evaluate the distribution of airborne microorganisms at underground shops in Seomyun and Nampodong, Pusan. The number of bacterial colonies on the nutrient agar plate plates were calculated by the open petri dish method for 30 minutes in indoor air of underground shops at every seasons in a year. There was no statistically significant difference between Seomyun and Nampodong in mean colony counts. Isolation rates of Gram positive rods was highest, and Gram positive cocci and Gram negative rods were followed. In Nampodong underground shops, Enterobacteriaceae strains was isolated. Mean colony counts according to seasons was higher at summer and autumn in Seomyun, and spring and winter in Nampodong. In near future, a study on the distribution of bacteria causing respiratory infection should be followed.

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Selection of High Yielding Mutant Strains for the Antifungal Antibiotics KRF-001 (항진균물질 KRF-001의 고생산성 변이주 분리)

  • 이항우;김무경
    • KSBB Journal
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    • v.9 no.4
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    • pp.378-384
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    • 1994
  • An improved method for the selective isolation of high-yielding mutant strains for the production of antifungal antibiotic KRF-001 was investigated. The mutant strain U. V 4, which produces high titer of KRF-001, was selected on the high potency agar plate after ultraviolet light irradiation. The U. V 4 strain produced 2-fold more KRF-001 than the mother strain in production media. Large scale fermentation was performed using the U. V 4 strain in 100$\ell$ fermenter. The antifungal antibiotic KRF-001 secreted into culture broth was detected by HPLC in 24hrs of fermentation.

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