• 제목/요약/키워드: Isolation method

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불확정 시스템에서의 복합성 이상검출 및 격리 (Composite Fault Detection and Isolation for Uncertain Systems)

  • 유호준;김대우;권오규
    • 대한전기학회논문지:전력기술부문A
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    • 제48권3호
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    • pp.257-262
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    • 1999
  • This paper proposes a composite fault detection and isolation method by combining the parameter estimation method[1] with the observer-based method[2] to take advantages of both methods. Some properties of the parameter estimation method and the observer-based method are revieved, and the composite algorithm is presented. To exemplify the performance of the method proposed, some simulations applied to remotely piloted vehicle are performed.

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식품으로부터 식중독 세균 검출을 위한 Real-time PCR에 적합한 DNA 추출 방법 비교 (Comparison of DNA isolation methods for detection of foodborne pathogens by real-time PCR from foods)

  • 구은정;김동호;오세욱
    • 한국식품과학회지
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    • 제48권4호
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    • pp.335-340
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    • 2016
  • 본 연구에서는 다양한 식품으로부터 식중독 세균의 DNA를 추출하는 효율을 비교 하였다. 사용된 DNA 추출 방법은 TaKaRa Kit를 이용하는 방법, PrepMan reagent를 이용하는 방법, boiling method, PEG를 이용한 alkaline method가 사용되었다. 비용절감이나 시간절약 면에서 boiling method나 PrepMan method도 고려할 수 있지만, column kit를 이용하는 TaKaRa kit가 효율적이라고 판단되었다. 또한 정성 시험법에서 적은 양의 균을 검출하기 위해 증균배양을 거치게 되는데 이때 사용되는 증균배지의 성분이 DNA 추출 후에도 잔류하여 saline과 비교하였을 때 DNA 추출효율이 낮은 결과를 나타내었다. 따라서 증균배지의 성분을 제거한 뒤 DNA를 추출하는 것이 PCR의 효율을 높일 수 있을 것으로 예상된다. 정량 시험법에서는 증균과정을 거치지 않아 균을 검출할 때 DNA의 추출 효율이 중요하기 때문에 DNA 추출 효율을 높이기 위한 가장 좋은 버퍼를 선정하고자 하였다. 그 결과 E. coli O157:H7에서는 saline이, S. aureus에서는 증류수를 버퍼로 사용했을 때 DNA 추출 효율이 가장 높은 것으로 나타났다.

SOC 테스팅을 위한 효율적인 부분 분리 링 (An Efficient Partial Isolation Ring Technique for SOC Testing)

  • 김문준;이영균;김석윤;장훈
    • 한국정보과학회논문지:시스템및이론
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    • 제28권10호
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    • pp.541-547
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    • 2001
  • 코이를 기초로 설계된 칩을 테스팅 하기 위해서는 각 코어로의 테스트 데이터 접근을 위해 완전히 분리 링 필요하다. 부분 분리 링은 코어를 둘러싸고 있는 분리 링의 크기를 줄이고 분리 링으로써의 역할을 모두 수행한다. 본 논문에서는 기존의 방법들보다 부분 분리 링의 크기를 더욱 작게 하고 해법을 찾는 데 걸리는 시간보다 줄일수 있는 효율적인 방법을 제시한다. 이를 위해 테스트 가능도에 기반을 둔 효율적인 순위 결정 기법을 적용하였으며 SOC 테스팅의 특성을 살린 정렬 기법을 적용하였다. 실험결과에서 기존의 방법들보다 본 논문에 제시된 방법이 실제로 유용하다는 것을 보여준다.

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조혈모세포이식 병동에 관한 문화기술지: 환자를 중심으로 (Ethnography on Isolation Unit for Hematopoietic Stem Cell Transplantation: Focusing on Patients)

  • 강영아;이명선
    • 종양간호연구
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    • 제9권1호
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    • pp.31-42
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    • 2009
  • Purpose: The purpose of the study was to understand how patients experience everyday life in an isolation unit for hematopoietic stem cell transplantation (HSCT). Method: The data were collected from 25 patients with HSCT at the isolation unit from January to March in 2008 in one general hospital in Korea. The data were collected by participant observations and ethnographic interviews and were analyzed using ethnographic method. Results: Four themes regarding environmental area emerged: 'barrier pulling up the drawbridge', 'very strange world', 'small and restricted space tied by IV and other treatment lines', and 'loud noise in a silent space.' Three themes regarding patients emerged: 'facing fear and anxiety', 'continuation of loneliness and lethargy', and 'compromising with a very long, dull, and boring time'. These themes describe how patients with HSCT suffer from continuous physical and psychosocial problems in a confined space, while endeavoring to control these problems and to search for hope for a new life. Conclusion: The results of the study provide an in-depth understanding of the experience and culture of patients in an isolation unit for HSCT. They would be used in developing practical programs to decrease patient's culture shock including fear and anxiety at isolation unit for HSCT.

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지반-구조물 상호작용 효과를 고려한 지진격리시스템이 적용된 원전 격납건물의 지진 취약도 평가 (Seismic Fragility Evaluation of Isolated NPP Containment Structure Considering Soil-Structure Interaction Effect)

  • 임승현;정형조;김민규;최인길
    • 한국지진공학회논문집
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    • 제17권2호
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    • pp.53-59
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    • 2013
  • Several researches have been studied to enhance the seismic performance of nuclear power plants (NPPs) by application of seismic isolation. If a seismic base isolation system is applied to NPPs, seismic performance of nuclear power plants should be reevaluated considering the soil-structure interaction effect. The seismic fragility analysis method has been used as a quantitative seismic safety evaluation method for the NPP structures and equipment. In this study, the seismic performance of an isolated NPP is evaluated by seismic fragility curves considering the soil-structure interaction effect. The designed seismic isolation is introduced to a containment building of Shin-Kori NPP which is KSNP (Korean Standard Nuclear Power Plant), to improve its seismic performance. The seismic analysis is performed considering the soil-structure interaction effect by using the linearized model of seismic isolation with SASSI (System for Analysis of Soil-Structure Interaction) program. Finally, the seismic fragility is evaluated based on soil-isolation-structure interaction analysis results.

Vertical isolation of a structure based on different states of seismic performance

  • Milanchian, Reza;Hosseini, Mahmood;Nekooei, Masoud
    • Earthquakes and Structures
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    • 제13권2호
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    • pp.103-118
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    • 2017
  • In vertical seismic isolation (VSI), a building is partitioned intentionally by vertical layers into two dynamically different substructures for seismic response reduction. Initially, a 1-story frame was partitioned into two substructures, interconnected by viscous and visco-elastic links, and seismic responses of the original and the vertically isolated structures (VIS) were obtained, considering a large number of stiffness and mass ratios of substructures with respect to the original structure. Color contour graphs were defined for presentation and investigation of large amounts of output results. Dynamic characteristics of the isolated structures were studied by considering the non-classical damping of the system, and then the effects of viscous and visco-elastic link parameters on the modal damping ratios were discussed. On this basis, three states of mass isolation, interactional state, and control mass were differentiated. Response history analyses were performed by Runge-Kutta numerical method. In these analyses, interaction of isolation ratios and link parameters, on response control of VIS was studied and the appropriate ranges for link parameters as well as the optimal ranges for isolation ratios were suggested. Results show that by using the VSI technique, seismic response reduction up to 50% in flexible substructure and even more in stiff substructure is achievable.

저궤도 인공위성의 센서 및 구동기 통합 고장검출 및 분리 기법 (An Integrated Fault Detection and Isolation Method for Sensors and Actuators of LEO Satellite)

  • 임준규;이준한;박찬국
    • 제어로봇시스템학회논문지
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    • 제17권11호
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    • pp.1117-1124
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    • 2011
  • An integrated fault detection and isolation method is proposed in this paper. The main objective of this paper is development fault detection, isolation and diagnosis algorithm based on the DKF (Decentralized Kalman Filter) and the bank of IMM (Interacting Multiple Model) filters using penalty scalar for both partial and total faults and the outlier detection algorithm for preventing false alarm also included. The proposed FDI (Fault Detection and Isolation) scheme is developed in four phases. In the first phase, the outlier detection filter is designed to prevent false alarm as a pre-filter. In the second phases, two local filters and master filter are designed to detect sensor faults. In the third phases, the proposed FDI scheme checks sensor residual to isolate sensor faults and 11 EKFs actuator fault models are designed to detect wherever actuator faults occur. In the last phases, four filters are designed to identify the fault type which is either the total fault or partial fault. The developed scheme can deal with not only sensor and actuator faults, but also preventing false alarm. An important feature of the proposed FDI scheme can decreases fault isolation time and figure out not only fault detection and isolation but also fault type identification. To verify the proposed FDI algorithm performance, the Simulator is also developed under the Matlab/Simulink environment.

지표수 및 해수로부터 Listeria monocytogenes의 분리 및 생존성 (Recovery and Survival of Listeria monocytogenes in Surface and Sea Water)

  • 양주;김도경;강호조
    • 대한수의학회지
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    • 제42권3호
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    • pp.327-333
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    • 2002
  • The study was carried out to examine the distribution and survival rate of Listeria monocytogenes (L monocytogenes) from various source of waters using improved isolation method. In comparision of enrichment media for isolation of L monocytogenes from water, the isolation rate and 50% detection limit of the pathogen were higher in UVM modified Listeria enrichment broth (UVM) than Listeria enrichment broth (LEB). On the other hand, when compared the selective media for isolation of the pathogen from water, the isolation rate was highest in culture at Oxford agar followed by Fraser agar, and LEB agar. In order to improve enrichment method, 100 ml of water samples with 0.1 CFU/ml of L monocytogenes was inoculated into 10 ml of UVM concentrated at 10-fold, and incubated for 24 h at $36^{\circ}C$. Isolated frequency of the pathogens in improved enrichment method completely corresponded with common (filter) method. Of a total mumber of 147 water samples from river, lake and sea, the pathogen was isolated from 1 of 39 (2.6%) river water samples and 1 of 75 (1.3%) sea water samples, but no pathogen was isolated from 33 lake water samples. Serotypes of 2 isolates were identified as type 1. L monocytogenes decreased in number from 7.2-7.4 to 4.2-4.7 log CFU/ml for 1 week poststorage (5 and $20^{\circ}C$), but the pathogens were able to be detected in river and sea water until 8 weeks after storage. However, in tap water, L monocytogenes were decreased to undetectable level after 2 weeks of storage.

차음시트의 음향투과손실 측정에 관한 연구 (A Study on the Sound Transmission Loss Measurement of Sound Isolation Sheets)

  • 이동훈;강문;이주원;정갑철;권영필
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2000년도 추계학술대회논문집
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    • pp.409-414
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    • 2000
  • A new impedance tube method is presented for the measurement of transmission loss of sound isolation sheets. The two-microphone method based on the sound decomposition theory proposed by Seybert and Ross is reviewed in this impedance tube method, which has been used for the determination of absorption coefficient of absorptive materials as well as transmission loss of automotive mufflers. Sound transmission losses for rubber, polyvinyl and asphalt sheets are measured in an impedance tube and reverberation room facility, respectively. By comparing two measurement methods, the reliability of impedance tube method used in this study is validated. From the experimental results, it is shown that the accuracy of sound isolation capability obtained by the impedance tube method depends upon the microphone spacing and the distance of the first microphone from the test sample surface.

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Dynamic assessment of the seismic isolation influence for various aircraft impact loads on the CPR1000 containment

  • Mei, Runyu;Li, Jianbo;Lin, Gao;Zhu, Xiuyun
    • Nuclear Engineering and Technology
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    • 제50권8호
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    • pp.1387-1401
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    • 2018
  • An aircraft impact (AI) on a nuclear power plant (NPP) is considered to be a beyond-design-basis event that draws considerable attention in the nuclear field. As some NPPs have already adopted the seismic isolation technology, and there are relevant standards to guide the application of this technology in future NPPs, a new challenge is that nuclear power engineers have to determine a reasonable method for performing AI analysis of base-isolated NPPs. Hence, dynamic influences of the seismic isolation on the vibration and structural damage characteristics of the base-isolated CPR1000 containment are studied under various aircraft loads. Unlike the seismic case, the impact energy of AI is directly impacting on the superstructure. Under the coupled influence of the seismic isolation and the various AI load, the flexible isolation layer weakens the constraint function of the foundation on the superstructure, the results show that the seismic isolation bearings will produce a large horizontal deformation if the AI load is large enough, the acceleration response at the base-mat will also be significantly affected by the different horizontal stiffness of the isolation bearing. These concerns require consideration during the design of the seismic isolation system.