• 제목/요약/키워드: Isolated from community

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Analysis of Plasmid pJP4 Horizontal Transfer and Its Impact on Bacterial Community Structure in Natural Soil

  • KIM TAE SUNG;KIM MI SOON;JUNG MEE KUM;JOE MIN JEONG;AHN JAE HYUNG;OH KYOUNG HEE;LEE MIN HYO;KIM MIN KYUN;KA JONG OK
    • Journal of Microbiology and Biotechnology
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    • 제15권2호
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    • pp.376-383
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    • 2005
  • Alcaligenes sp. JMP228 carrying 2,4­dichlorophenoxyacetic acid (2,4-D) degradative plasmid pJP4 was inoculated into natural soil, and transfer of the plasmid pJP4 to indigenous soil bacteria was investigated with and without 2,4-D amendment. Plasmid pJP4 transfer was enhanced in the soils treated with 2,4-D, compared to the soils not amended with 2,4-D. Several different transconjugants were isolated from the soils treated with 2,4-D, while no indigenous transconjugants were obtained from the unamended soils. Inoculation of the soils with both the donor Alcaligenes sp. JMP228/pJP4 and a recipient Burkholderia cepacia DBO 1 produced less diverse transconjugants than the soils inoculated with the donor alone. Repetitive extragenic palindromic-polymerase chain reaction (REP-PCR) analysis of the transconjugants exhibited seven distinct genomic DNA fingerprints. Analysis of 16S rDNA sequences indicated that the transconjugants were related to members of the genera Burkholderia and Pandoraea. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that inoculation of the donor caused clear changes in the bacterial community structure of the 2,4-D­amended soils. The new 16S rRNA gene bands in the DGGE profile corresponded with the 16S rRNA genes of 2,4-D­degrading transconjugants isolated from the soil. The results indicate that introduction of the 2,4-D degradative plasmid as Alcaligenes sp. JMP228/pJP4 has a substantial impact on the bacterial community structure in the 2,4-D-amended soil.

A New Record of Neosartorya aureola Isolated from Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • 한국균학회지
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    • 제43권3호
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    • pp.191-195
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    • 2015
  • A new species of Neosartorya was recovered during investigation of the fungal community in soil samples collected from different locations in Korea; Neosartorya aureola KNU14-7 was isolated for the first time from field soil in Korea and identified based on the internal transcribed spacer region of rDNA and morphological characteristics. The species has not been officially reported from Korea and we report it here with description and figures.

독도서식 식물근권에서 분리한 포자형성세균과 질소고정세균의 군집구조 분석 (Analysis of Endospore-forming Bacteria or Nitrogen-fixing Bacteria Community Isolated from Plants Rhizosphere in Dokdo Island)

  • 전선애;성혜리;박유미;박재홍;김사열
    • 한국미생물·생명공학회지
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    • 제37권3호
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    • pp.189-196
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    • 2009
  • Bacteria were isolated from roots of plants belonging to family Solanaceae and Gramineae, inhabited in Dokdo island. Fifty six endospore-forming bacteria grown on tryptic soy broth (TSB) agar medium and 23 nitrogen-fixing bacteria (NFB) grown on nitrogen free agar medium were isolated, respectively. The isolates were partially identified by analyzing the 16S rDNA and categorized into phylogenetic groups. The 16S rDNA sequences of each identified isolates were compared with sequences of each type strains to analyze phylogenetic relationship by phylogenetic tree. As a result, endospore-forming bacteria and nitrogen-fixing bacteria were classified into 4 and 6 lineage groups, respectively. Among these isolated, 18 were presumed to be novel species candidates based on the similarity (lower than 98%) analysis of the l6S rDNA sequences.

Community Structure of Arbuscular Mycorrhizal Fungi in Upo Wetland, Korea

  • Park, Hyeok;Ko, Kang-Moon;Eom, Ahn-Heum
    • 한국균학회소식:학술대회논문집
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    • 한국균학회 2018년도 춘계학술대회 및 임시총회
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    • pp.35-35
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    • 2018
  • Arbuscular mycorrhizal fungi (AMF) are one of the most widespread symbionts globally. Owing to their enhanced nutrient absorption capacity, AMF significantly contribute to the survival of individual plants and the ecosystem functioning. Community structures of AMF are affected by many environmental factors Inland wetlands have a different environment from common forest soils, therefore, plants inhabiting wetlands may have characteristic AMF communities. The purpose of this study was to compare the AMF communities in wetlands, among the species of host plants. We sampled the roots of 3 host plant species, Phragmites communis, Miscanthus sacchariflorus, and Trisetum bifidum with rhizospheres from 3 isolated areas in Upo wetland, Korea. We extracted DNA from AMF spores in rhizospheres and the roots of 3 plant species. We amplified 18S rDNA of AMF using AMF specific primer. As a result, we confirmed 9 species from 5 genera in AMF spores, and 5 species from 3 genera in plant roots. Funneliformis caledonium was the most dominant species in field soils, on the other hand, Diversispora aurantia was the most dominant species in plant roots. We confirmed that species diversity and abundance of AMF communities were different among host plant species. These results showed that the AMF community had specific to host plants in the inland wetland.

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바이칼 호수에 서식하는 담수 스폰지 내 공생세균의 분리 (Isolation of Bacteria Associated with Fresh Sponges in Lake Baikal)

  • 조안나;김주영;안태석
    • 생태와환경
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    • 제47권spc호
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    • pp.39-47
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    • 2014
  • 바이칼호에 서식하는 2종의 스폰지 체내 및 주변 물로부터 92개의 저온성 균주를 분리하고 각 균주들의 기질 분해능을 조사하였다. 그 결과 섬유소와 지방에 대한 분해 활성도를 갖는 균주는 38.0, 34.8%로 비교적 적었으나 전분과 단백질 분해 활성도를 갖는 균주는 78.3, 57.6%로 높은 비율로 나타났다. 분리한 세균을 염기서열의 유사도에 따라 분류하기 위하여 Genomic Fingerprinting을 실시한 후 31개 균주를 선별하여 동정한 결과, Baikalospongia sp.에서 분리한 13균주는 모두 Pseudomonas속으로 확인된 반면, Lubomirskia sp.에서 분리한 14균주는 Pseudomonas ssp., Buttiauxella agrestis, Pseudomonas fluorescens, Yersinia ruckeri, Bacillus ssp., Paenibacillus ssp., Bacillus thuringiensis, Bacillus simplex, Brevibacterium ssp., Acinetobacter lwoffii로 다양하게 동정되었다. 그러나 총 31개 균주 중 18개가 Pseudomonas속으로 동정된 것은 타감작용에 의한 다른 세균 성장의 방해 때문으로 평가되며, 이러한 일반적인 배양 방법의 한계점을 극복하기 위해서는 스폰지의 서식처와 세균의 검출 방법에 대하여 보다 다양한 심층적인 연구가 이루어져야 할 것으로 생각된다.

보건진료원의 지역사회 몰입과정 (Community Health Practitioner's Commitment into Community : on the Aspect of Primary Health Care)

  • 윤순녕;김영임;최정명
    • 지역사회간호학회지
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    • 제6권2호
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    • pp.173-182
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    • 1995
  • Primary health care(PHC) has been established since A Health Law for rural residents has been legislated in 1980 following the WHO declaration, 'Health for All 2000'. in 1978. PHC services are presently assumed to be provided by 2038 Community Health Practitioners(CHP) to about 28% out of rural population in Korea. Most CHPs have confronted the adaptation process to the community being practiced although a CHP's role is to evoke community participation for the improvement of their health by themselves. So the purpose of this study is to describe and explain of the commitment of CHPs into the community. Data were collected by direct interview and tape-recording under subjects' permission till theoretical saturation were occured from 6 CHPs. The subjects were 41 years old and have served in the community for 9 years in average. Main questions and concepts were explored from data according to the procedure of the grounded theory methodology. The results are as follows. 1) The number of the main concepts were twenty four that identified Motive, Desire, Personal characteristics, Unfamiliarity, Denial, Feeling of isolation, Self-sacrifice, Kindness, Patience, Assimilation, Respect for the residents, Support by the family, Support by the residents, Achievement, Acceptance of realities, Use of resources, Inducement of cooperation from the residents, Changes of the difference from time orientation between CHP and residents, Attitude as a official, Technical support, Cost management, Satisfaction level, Acknowledgement by the residents and discrepancy. 2) The twenty four concepts were categorized to seven groups such as Motivation, Feeling of Heterogeneity, Self-discipline, Social support, Induced changes in the attitudes of residents, Familarity and Persistent discrepancy. 3) The categorized groups were analyzed on the base of the Causal Conditions, Central Phenomena, Contexts, Intervening Conditions, Action / Interaction Strategies, and Consequences. Central phenomenon in this study was identified to be the feeling of heterogeneity. Community health practitioners experienced unfamiliarity and denial from the community and felt themselves isolated in the first. In time, they won the trust of residents by their efforts including self-sacrifice, kindness, patience, and assimilation. Afterward, practitioners got self-confidence and familiarity with lesser feeling of heterogeneity. Nevertheless, practitioners could not commit themselves completely because of the persistent discrepancy between CHP and residents. 4) On the commitment process, the CHPs' feeling of heterogeneity were decresed and social support increesed and newly evolved induced change of residents through the continuous interaction between CHP and them The contribution of this study would be concluded as follows. 1) It is expected that effective strategies for more rapid committment into the community can be developed based on this study. 2) More easy committment would be possible for the newly appointed CHP through understanding of the committment process identified on this study.

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도시노인의 여가공간과 생활세계 (Life World and Experiences of Spaces of Urban Elderly in Korea)

  • 한경혜;윤성은
    • 한국지역사회생활과학회지
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    • 제20권1호
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    • pp.103-121
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    • 2009
  • Increased consensus among gerontologists exist on the need to pay greater attention to the reciprocal relationship between the social and spatial in order to understand the construction of aged identity and everyday lives of old people. With urbanization, spaces are increasingly age-graded and as a consequence, urban elders are socially isolated from the community. In this study, we examine the social interaction patterns in various places specifically designated for the elderly, such as the Senior Center, Senior Welfare Center, and Hall for the Aged in Seoul. Main purpose of this study is to understand everyday life experiences of space the elderly people residing in the city go through. Data were gathered through qualitative, case study method. Results show that such spaces were important sites for social interaction for urban elderly who lacked social spaces after retirement and active parenting role. Also, it was shown that each place presents different contexts for social interactions and certain components of social class differences existed. Heterogeneity within the participants of each place was also observed. Quite strong stereotypes about certain places were also observed among the urban elders. Based on these results, importance of developing a 'community perspective' in the study of old age is discussed.

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Pseudomonas putida Strain 17 Isolated from Replant Soil Promotes Tomato Growth and Inhibits Conidial Germination of Soilborne Plant Pathogens

  • Lee, Sang-Woo;Ahn, Il-Pyung;Lim, Jae-Wook;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • 제21권3호
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    • pp.244-251
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    • 2005
  • The induction of growth promotion on numerous crops by rhizobacteria is a well documented phenomenon. In case of tomato (Lycopersicon esculentum), fruit yield is higher in replant soil than that in fresh soil. To investigate what kind of rhizobacterium is involved, microbial community in rhizosphere and on rhizoplane of tomato plants from each soil was analyzed by dilution plating on selective media. Many Gram-negative bacteria and actinomycetes were isolated from tomato in replant soil. One Gram-negative rhizobacterium isolated was identified as Pseudomonas putida based on its biochemical characteristics, fatty acid methyl ester analysis and 16S rDNA sequence. This bacterium designated strain 17 inhibited the growth of Pseudomonas corrugata, and increased growth of tomato seedlings. In addition, its culture filtrate inhibited conidial germination of plant-pathogenic fungi such as Fusarium oxysporum f. sp. radicis-lycopersici, F. oxysporum f. sp. cucumerinum, and Nectria radicicola. Scanning electron microscopy revealed strain 17 colonized and persisted on the epidermal surfaces of tomato radicles and roots. These results suggest that P. putida strain 17 may serve as a biological control agent to suppress multiple soil-borne diseases for tomato plants. Increased microbial populations that suppress deleterious microorganisms including pathogens could be one of the major factors in increased tomato yield in replant soil.

지역사회 내 일반인 및 동물에서 methicillin 내성 Staphylococcus aureus의 유전학적 특성 (Genetic characterization of methicillin-resistant Staphylococcus aureus from humans and animals within the community)

  • 조재근;김진현;성명숙;김기석
    • 대한수의학회지
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    • 제51권4호
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    • pp.267-275
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    • 2011
  • Methicillin-resistant Staphylococcus (S.) aureus (MRSA) is one of the most important nosocomial pathogens worldwide and the emergence of this strain has become a major clinical problem. In this study, we investigated the prevalence of MRSA and their genetic characteristics in 69 S. aureus isolated from humans and animals. In human isolates, higher antimicrobial resistance rates were observed against penicillin (80.6%), followed by erythromycin (11.9%) and tetracycline (9.0%). All of them were susceptible to clindamycin, enrofloxacin, novobiocin, pirlimycin, trimethoprim/sulfamethoxazole and vancomycin. The resistance patterns in animal isolates were similar to those of human isolates. Two (2.9%) MRSA strains were isolated from human (n = 1) and animal (n = 1), and these isolates were confirmed as carrying the mecA gene. One isolate originating from human was resistant to 7 drugs and the other isolate derived from animal was resistant to 11 drugs. Staphylococcal cassette chromosome mec (SCCmec) variant IIIB was identified in animal isolate but SCCmec type of an isolate from human was not exactly determined. Two MRSA isolates showed unrelated PFGE pattern between them. Our results indicated although the frequency of MRSA isolates from humans and animals was low, a continuous surveillance and monitoring should be called for to prevent the contamination and spread of MRSA in the community. To our knowledge, this is the first time that SCCmec type variant IIIB was detected from animals in Korea.

천수만 해역 세균 군집의 수리학적 분석 (Numerical Analysis of Bacterial Community in Cheonsu bay)

  • 정현미;김명운;이건형;김상종
    • 미생물학회지
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    • 제27권3호
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    • pp.265-271
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    • 1989
  • 서해안의 대표적 내만인 천수만 해역에서 4계절간 분리한 세균들을 대상으로 수리학적 분류를 설시하였다. 총 48가지의 형태학적, 생리적, 생화학적 실험 결과 분리된 계절에 따라 온도에 대한 서로 다른 적응 양상을 보여 주었으며, 전체적으로 다양한 환경변화에 높은 생존 능력을 보여주였다. 각 세균을 동정한 결과 Enterobacteriaceae, Aeromonas Pseudomonas와 Vibrio 등이 우점종으로 나타났으며, 계절별로는 봄 가을에 Aeromonas가, 여름 및 겨울에는 각각 Pseudom monas 와 Enterobacteriaceae가 제l우점종임이 발견되었다 Cluster analysis 결과 젠체 세규 군집은 75%의 유사도 수1 에서 총 29개의 phenetic group으로 나뉘어졌으며 각 group별로 계절적 구분이 뚜렷하였다. 전체적으로 계절에 놔른 서로 다른 생리적 특성과 종 구성이 전반적인 생태계에의 다양성과 안정성을 높여주는 것으로 나타났다.

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