• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.1
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• pp.22-27
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• 2004

In the present study, the protective effects of Artemisia capillaris (AC) on the DNA damage induced by $^{60}$ Co ${\gamma}$-rays were evaluated using alkaline single-cell gel electrophoresis (SCGE, comet assay) in the mouse peripheral lymphocytes and micronuclei (MN) formation test in the Chinese hamster ovary (CHO) cells. We also investigated the effect of AC on 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation in the mouse liver and thymus exposed to ${\gamma}$-ray, The tail moment and the frequency of MN, which were markers of DNA damage in the SCGE and MN formation test, were decreased in the groups treated with AC extract before exposure to 200 cGy of ${\gamma}$-ray. We also observed its activities, lowering 8-OHdG level, an index of oxidative DNA damage, in the groups treated with AC extract before whole body ${\gamma}$-irradiation (800 cGy). It is plausible that scavenging of free radicals by AC may have played an important role in providing the protection against the radiation-induced damage to the DNA. These results indicated that AC protects the DNA damage induced by ${\gamma}$-rays and might be a useful radioprotector, especially since it is a relatively nontoxic product.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.4
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• pp.624-632
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• 2006

In recent years, xenon plasma arc lamp was introduced for high-intensity curing of composite filling materials in direct resin restorations. In this study, two types of restorative materials, namely composites point $4^{(R)}$ and $Z250^{(R)}$ were selected and curing was conducted using a conventional halogen light and two plama curing lights. Two different resin composites were cured using the different units($Flipo^{(R)}$, Ultra-lite 180A, and $TriLight^{(R)}$) and tested for microhardness. The purpose of this study was to test the hypothesis that exposure to a plasma curing lamp for 3, 6. 9 seconds is equivalent to 20 or 40 seconds of irradiation using a conventional halogen curing unit. 1. $Flipo^{(R)}$ and Ultra-lite 180A were able to polymerize point $4^{(R)}$ at 6 seconds to a degree equal to that of the $TriLight^{(R)}$(control) at 40 seconds. 2. $Flipo^{(R)}$ was able to polymerize $Z250^{(R)}$ at 9 seconds to a degree equal to that of the $TriLight^{(R)}$(control) on the bottom surface at 20 seconds. whereas Ultra-lite 180A could not do. 3. Two plasma curing units were able to cure the test-composites with bottom/top ratios approximately 61% to 96% at 3 to 9 seconds. There were some differences between the two composite brands, with $Z250^{(R)}$ displaying less difference between top and bottom hardness values. For point $4^{(R)}$ and $Z250^{(R)}$, at least 6 or 9 seconds were necessary to produce microhardness equivalent to that of the $TriLight^{(R)}$ curing at 20 or 40 seconds.

• Journal of Korean Academy of Nursing
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• v.30 no.2
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• pp.514-526
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• 2000

The active treatment phase in preparation for bone marrow transplantation(BMT) of che- motherapy regimen and total body irradiation (TBI) containing regimen requires considerable teaching. There have been researches that are related to treatment onto BMT patients and to psychological change during BMT process. However, it was hard to find researches focused on learning needs of patients undergoing BMT. The purpose of this study was to provide the basic data for effective educational program about BMT by investigating the learning needs in patients undergoing BMT. The subjects consisted of 90 BMT patients have been admitted to the department of BMT at three university hospitals. Data were obtained from October 1998 to March 1999 and analyzed by SAS program for unpaired t-test, ANOVA, Duncan test. The results were as follows : 1. Learning needs related to demographic characteristics was identified as below. That of male was higher than that of female. That of under age 29, unmarried, religious and university graduated group was higher than that of opposite group but it didn't show significant difference. Learning needs of group of patients who were employed was significantly higher then that of unemployed patients. 2. According to types of diagnosis, learning needs of myelodysplastic syndrome(MDS) patients was the higher than that of others, but admission frequency was the least. Learning needs of unrelated matched BMT(UBMT) patients was higher than that of autologous BMT patients. However, it didn't show significant difference. With regard to learning needs according to process of BMT, learning needs of Pre- BMT period or Post-BMT period was significantly higher than that of BMT day. 3. Learning needs related to BMT was relatively high (total mean: 3.11 of 4.0). The order of the mean score of leaning needs was shown as follows : Restricted activities after discharge, Relapse symptom, Complications of BMT, Kinds of available drugs at home. Therefore the learning needs that is related to life after discharge and to relapse and complications after BMT was high. 4. Learning needs related to radiation therapy was high (total mean: 3.35 of 4.0). The learning needs in radiation therapy items was the Skin care of radiation therapy and Purpose of radiation therapy. 5. Learning needs related to graft versus host disease(GVHD) therapy was high (total mean: 3.55 of 4.0). The highest learning needs in GVHD therapy items was the Preventive method GVHD. less admission frequency and UBMT patients. It is necessary that education for BMT patients should be focused on life after discharge and on relapse and complications after BMT. Especially education for allogeneic BMT patients should be emphasized on GVHD. For all of these, it is necessary to develop systematic and concrete educational program.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.348-353
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• 1996

Mutation tachniques inducing more useful mutations and reducing somatic effects need to be improved for crop breeding. Seeds of barley varieties ; Dema, Grosso were treated with two types of mutagens ; 1) chemical treatment: single treatment or double treatment of two mutagens (N-nitroso-N-methylurea ; MNH, Sodium Azide; NaN$_3$) 2) gamma ray irradiation treatment. After treatment, half of seeds were used for germination test and half of seeds were sown to the field. With the higher dose of mutagen both chemical and gamma ray were plants treated, the higher rate of growth reduction rate was in M$_1$ seedling. In chemical treatment, germination rate of seeds, growth rate of coleoptile and root in double treatment of chemical mutagens were better than single treatments, especially in same dose. Growth inhibition rate of plant in double treatment of 1.0mM MNH(0.5mM MNH + 0.5mM MNH), for example, were less than one of plants of single treatment of 1.0mM MNH in pot and petri dish test. Growth reduction rate of culm and fertility rate in M$_1$ plants double treated in same dose of single treatment were also less than single one. With the higher dose of mutagen both chemical and gamma ray were plants treated, the higher frequency of chlorophyll mutants was in M$_2$ seedling. The rate of chlorophyll mutants in double treatment of chemical mutagens were higher than single treatment. Double treatment methods can be a improved method for induction of new good mutants, which were induced more useful mutations and reduced harmful somatic effects.

• Journal of Electrical Engineering and Technology
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• v.13 no.2
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• pp.936-942
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• 2018

The diagnostic multi-leaf collimator preventing unnecessary dose from entering into patients during the diagnostic examination was made in this study. The movement of the entire 50 leaves was embodied with the group of 25 ones thereof configured in a pair facing each other on the left and right of the median line. Dimensions of the length, width, and height of each shielding leaf were $5{\times}0.5{\times}0.5cm^3$ resulting in the maximum boost field of $10{\times}10cm^2$. The material of multi-leaf collimator had the excellence on the machinability with the use of the SKD-11 alloy tool steel having the high wear resistance against frequent movement, and it was devised to control both-side's shielding leaves by moving 2 motors unlike existing remedial multi-leaf collimator that use as many motors as the number of 50 shielding leaves. Thereafter, the transmission dose of leaves, cross-leaf leakage dose, and inter-leaf leakage dose were measured by the developed multi-leaf collimator attached to X-ray equipment. An ionization chamber was used to detect doses there from, and the comparative analysis was carried out by means of the radiographic film that was easy to detect the dose leakage in between each leaf. Results obtained from the test conducted in comparative analysis yielded approximately 98%, 96%, and 94% of shielding efficiency realized at each level of energy of 80kV, 100kV, and 120kV it was confirmed there was no dose leakage resulted from the varied level of irradiation energy. Thus the multi-leaf collimator to be developed based on this study is thought that it could fully reduce the unnecessary dose to patients in the diagnostic test and the shielding efficiency thereof is expected to be increasing if it is made in a miniaturized form with a way of increasing the thickness of each leaf later for an extended application to general diagnostic purposes.

• PNF and Movement
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• v.7 no.3
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• pp.17-27
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• 2009

The purpose of this study was to investigate the effect of close kinetic chain(CKC) and open kinetic chain(OKC) posion on proprioceptive neuromuscular facilitation applied to the unilateral upper extremity on the muscle activation of lower extremity. All subjects were randomly assigned to two groups: open kinetic chain group(n=5),closed kinetic chain group(n=5). All participants were PNF patterns applied on the unilateral upper extremity in all subjects were the kinetic chain(CKC) and open kinetic chain(OKC) posion on flexion/abduction/external rotation. The hold and approximation techniques for the irradiation were applied to end range. All measurements for each subject took the following tests: pre-test, post - test in 4weeks, post-testin 8weeks. EMG data was collected from the vastus medialis, tibialis anterior, biceps femoris, and gastrocnemius muscle of both lower extreamity using surface EMG system, Each EMG value in individual muscle was normalized for maximal voluntary contraction. The data were analyzed using Two-way analysis of variance(ANOVA) with repeated measures to determine the statistical significances. The results of this study are summarized as follows. First, during for close kinetic chain(CKC) and open kinetic chain(OKC) posion on PNF pattern application, all of the %MVIC values of close kinetic chain and open kinetic chain posion increased sign ificantly compared(p<0.05). Second, The close kinetic chain(CKC) and open kinetic chain(OKC) posion on PNF pattern application was significantly increased with in the intervention period(p<.05). Third, there was a no significant open kinetic chain posion on PNF pattern application of sing muscle group with in the intervention period.(p<0.05) there was a significant close kinetic chain posion on PNF pattern application of sing muscle group with with in the intervention period(p<0.05). Forth, interaction of the exercise position and muscle was also significant. Post-hoc tests revealed that the activation levels of vastus medialis muscle and tibialis anterior muscle was higher in the closed kinetic chain position(p<.05). that the activation levels of vastus medialis and gastrocnemius muscle was higher in the open kinetic chain position(p<.05). In conclusion, it was found that the application of PNF patterns to the unilateral upper extremity effect on the muscle activation of lower extremity and both open kinetic chain exercise and closed kinetic chain exercise was significantly increased muscle activity. Further studies are needed to analyzed long term effects and subjects resulting from these changed.

• Radiation Oncology Journal
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• v.4 no.2
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• pp.99-105
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• 1986

Optimal balance between control probability and risk of complication is emphasized even in present time, Although certain incidence of intestinal injury is accepted as an inevitable consequence after abdominopelvic irradiation, these complications still remain as problems. 60 mice were irradiated with 250 kVp orthovoltage x-ray machine and $200rad{\times}5/wk$ regimen. Histpathologic findings of colorectum and the relationship with occult blood test were analyzed and possible tolerable dose which would be safe from permanent complication was also estimated. Followings are the results: Mild mucosal and submucosal edema were observed in 1,000 rad irradiated group. Congestion of small vessels was prominent in 2,000 rad irradiated group and infiltration of inflammatory cells was observed in 3,000 rad irradiated group. Denuded mucosa was observed in 3,000 rad irradiated group. Occult blood test is not a proper indicator for rectal denuding or rectal ulcer, but our results suggest the possibility of using this as a relative scale of intestinal damage. Mitotic figures of crypt cells were observed even in 5,000 rad irradiated group, these suggest that the repair capacity of crypt cells are still functioning.

• The Korean Journal of Nuclear Medicine
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• v.5 no.2
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• pp.19-40
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• 1971

Since the iron balance is maintained by regulated intestinal absorption rather than regulated excretion, there have been many reports concerning the factors which may influence the intestinal iron absorption. As the liver is the largest iron storage organ of the body, any hepatocellular damage may result in disturbances in iron metabolism, e,g., frequent co-existence of hemochromatosis and liver cirrhosis, or elevated serum iron level and increased iron absorption rate in patients with infectious hepatitis or cirrhosis. In one effort to demonstrate the influence of hepatocellular damage on intestinal iron absortion, the iron absorption rate was measured in the rabbits whose livers were injured by a single subcutaneous injection of carbon tetrachloride (doses ranging from 0.15 to 0.5cc per kg of body weight) or by a single irradiation of 2,000 to 16,000 rads with $^{60}Co$ on the liver locally. A single oral dose of $1{\mu}Ci\;of\;^{59}Fe$-citrate with 0.5mg of ferrous citrate was fed in the fasting state, 24 hours after hepatic damage had been induced, without any reducing or chelating agents, and stool was collected for one week thereafter. Serum iron levels, together with conventional liver function tests, were measured at 24, 48, 72, 120 and 168 hours after liver damage had been induced. All animals were sacrificed upon the completing of the one week's test period and tissue specimens were prepared for H-E and Gomori's iron stain. Following are the results. 1. Normal iron absorption rate of the rabbit was $41.72{\pm}3.61%$ when 0.5mg of iron was given in the fasting state, as measured by subtracting the amount recovered in stool collected for 7 days from the amount given. The test period of 7 days is adequate, for only 1% of the iron given was excreted thereafter. 2. The intestinal iron absorption rate and serum iron level were significantly increased when the animal was poisoned by a single subcutaneous injection of 0.15cc. per kg. of body weight of carbon tetrachloride or more, or the liver was irradiated with a single dose of 12,000 rads or more. The results of liver function tests which were done simultaneously remained within normal limit except SGOT and SGPT which were somewhat increased. 3. In each case, there has been good correlation between the extent of liver cell damage and degree of increased iron absorption rate or serum iron level. 4. The method of liver damage appeared to make no obvious difference in the pattern of iron deposit in liver. This may be partly due to the fact that tissue specimens were obtained too late, for by this time the elevated serum iron level had returned within normal range and the pathological changes were almost healed. 5. The possible factors and relationship between intestinal iron absorption and hepatic parenchymal cell damage has been discussed.

• Journal of Radiation Industry
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• v.5 no.3
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• pp.211-220
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• 2011

This study was carried out to develop salt tolerant varities of rapeseed (Brassica napus L.) which can be grown in the high salty reclaimed land. The seeds of three varieties 'Naehan', 'Tammi', and 'Halla' were treated by proton ion beams and gamma rays with 0 to 2,000 Gy. For the selection of salt tolerant lines, emergence and survival rate, and growth characteristics of $M_2$ to $M_4$ generations were investigated in the Saemangeum reclaimed fields with the different salt concentrations. The lines with potential salt tolerance were selected in the $M_4$ generation and tested indoor for their growth characteristics. There was no significant changes in the soil pH for $M_4$ generation during growth period. However, soil EC was higher in early spring than sowing period (mid October). In $M_4$ generation test, the seeds of original and selected line showed high rates of emergence and survival, as determined one month after sowing. After wintering, however, the original varieties showed the significant reduction in the survival rate, while the selected lines showed a higher survival rate and good growth, leading to the completion of their life cycle. Consequently we selected 9 lines from $M_4$ generation with better performance in growth and yield. Soil EC was $2.8{\sim}4.3dS{\cdot}m^{-1}$ during $M_4$ generation growth period. The laboratory test of the lines selected from $M_4$ generation was made for their salt tolerance potential. The selected lines showed higher chlorophyll and proline contents than the original varieties. There was also no significant difference in the emergence rate of seed between the original and selected varieties. In 200 mM natural sea salt, the $N{\gamma}600-21-1-641$ line derived from 'Naehan' was the highest in growth rate, leaf chlorophyll and proline contents. $T{\gamma}800-20-2-461$ line derived from 'Tammi' didn't show significant difference in growth rate compared to original variety in 200 mM and withered in 250 mM like other lines as time passed. $H{\gamma}200-7-1-740$ line showed similar growth and chlorophyll content compared to its original variety.

• Polymer(Korea)
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• v.38 no.1
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• pp.69-73
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• 2014

In this study, polysaccharide-poly(vinyl alcohol) (PVA) hydrogels were prepared by using ${\gamma}$-ray and evaluated for potential application as an anti-inflammation patch. Ulmus davidiana var. japonica (UD), one of polysaccharides has been particularly used as an oriental remedy for the treatment of inflammation and ulcers. PVA as a biocompatible polymer and glycerin as a moisturizer were blended with the UD, and its hydrogels were prepared by radiation crosslinking. Characterizations for UD hydrogels were performed by using cytotoxicity assay, antioxidant activity test, and physicochemical test such as gel fraction ratio, and swelling behavior. The results showed that these UD hydrogels had excellent physical properties, anti-inflammation activity, and non-cytotoxicity on the cells. Therefore, these polysaccharide based-UD hydrogels can be effectively used as an inflammation patch.