• Title/Summary/Keyword: Irradiation production

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Investigation of Potential Photoreactivation of Pseudomonas aeruginosa after LP or MP UV Irradiation (저압 및 중압 자외선 조사에 의해 불활성화된 Pseudomonas aeruginosa의 광회복능 조사)

  • Mun, Sung-Min;Cho, Min;Yoon, Je-Yong
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.5
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    • pp.755-761
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    • 2006
  • Recently, there is growing interest in ultraviolet (UV) irradiation as a disinfection technic in drinking water production due to its effectiveness to inactivate microorganisms such as Crytosporidium parvum without forming disinfection byproducts. However, UV disinfection is known for its drawback such as photoreactivation. Despite many works concerning the photoreactivation, most of works were focused on indicator or non pathogenic microorganisms. The objective of this study is to examine the photoreactivation of Pseudomonas aeruginosa which is an opportunistic pathogen as UV radiation by LP and MP UV lamp was applied. The result showed that P. aeruginosa had high photo repair efficiency regardless of the type of UV irradiation. Both of the effective log repair values of LP and MP UV irradiation were found approximately 2.6 log. In addition, photo repaired P. aeruginosa was not significantly different in forming biofilm in comparison with non treated P. aeruginosa.

Production of Haploids from Proton Ion and Gamma-Ray Irradiation Treated $M_2$ Generation of Isolated Microspores in Brassica napus L. ssp. oleifera (앙성자 및 감마선을 처리한 유채 $M_2$ 세대의 소포자로부터 반수체 배발생)

  • Kim, Kwang-Soo;Li, Mei-Yang;Jang, Young-Seok;Park, Yoon-Jung;Bang, Jin-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.2
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    • pp.150-155
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    • 2008
  • This experiment was carried out to investigate the effect of proton ion and gamma-ray irradiation on microspore culture of the flower buds of $M_2$ generation in winter type of Brassica napus L. ssp. oleifera. The seeds of three rape varieties, 'Halla', 'Naehan' and 'Tammi' were pretreated with proton ion and gamma-ray 400 Gy and 600 Gy, respectively. When microspore culture techniques were used, embryogenesis was increased in some varieties by proton ion and gamma-ray irradiation treated flower buds of $M_2$ generation than control. In genotypes 'Naehan' showed the highest embryo production frequency, but 'Tammi' showed lowest embryo production frequency. Some of the embryoids developed directly into plantlets, whereas others developed abnormally multilobe. Plants were regenerated and successfully acclimatized in pots.

Reduction of Immune Toxicity of LPS by Gamma Irradiation (감마선 조사에 의한 LPS의 면역 독성 저감화)

  • Park, Jong-Heum;Sung, Nak-Yun;Byun, Eui-Beak;Song, Du-Sup;Song, Beom-Seok;Kim, Jaekyung;Lee, Ju-Woon;Park, Sang-Hyun;Kim, Jae-Hun;Kang, Geon-Ok;Yoo, Young-Choon
    • Journal of Radiation Industry
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    • v.6 no.3
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    • pp.217-221
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    • 2012
  • The purpose of this study is to reduce the immune toxicity of lipopolysaccharide (LPS) by gamma irradiation. LPS was gamma-irradiated at the various doses of 20, 100 and 200 kGy and then evaluated on the immune toxicity through the cell proliferation, nitricoxide production and cytokine release. Cell proliferation significantly decreased in the intact LPS treated groups, whereas gamma-irradiated LPS treated group were not reduced the cell proliferation. Similarly, the production of nitric oxide and cytokine showed the high levels in the intact LPS treated group. However, gamma-irradiated LPS treated group remarkably decreased the production of nitric oxide and cytokine in dose-dependent manner. Therefore, gamma irradiation may effective method to reduce the immune toxicity of LPS.

Effect of Gamma Irradiation on the Splenocyte Proliferation and Cytokine Production of Chaga Mushroom Hot Water Extract (방사선 조사된 차가버섯 열수 추출물의 비장세포 증식능 및 사이토카인 발현에 미치는 영향)

  • Sung, Nak-Yun;Kim, Jae-Hun;Choi, Jong-Il;Song, Beom-Seok;Kim, Jae-Kyung;Park, Jong-Heum;Kim, Jin-Kyu;Lee, Ju-Woon
    • Journal of Radiation Industry
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    • v.5 no.2
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    • pp.169-173
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    • 2011
  • This study investigated the effect of gamma irradiation on immune enhancing activity of Chaga mushroom extract (CME). CME was prepared by hot water extraction at $70^{\circ}C$ for 4 hours and lyophilized. Lyophilized CME powder was dissolved with deionized water at $10mg\;ml^{-1}$ and then irradiated at the doses of 10, 30 and 50 kGy by cobalt 60 gamma irradiator. The gamma-irradiated and non-irradiated CME were treated into the splenocyte separated from mouse. Cell proliferation and cytokine production of the immune cells were increased by gamma-irradiated CME and these increases were more prominent when CME was irradiated at higher doses. Therefore, it is considered that gamma irradiation can be an effective method for improvement of the immunomodulating activity Chaga mushroom extract.

The Effect of Irradiation on Meat Products

  • Yea-Ji Kim;Ji Yoon Cha;Tae-Kyung Kim;Jae Hoon Lee;Samooel Jung;Yun-Sang Choi
    • Food Science of Animal Resources
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    • v.44 no.4
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    • pp.779-789
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    • 2024
  • The effects of irradiation on meat constituents including water, proteins, and lipids are multifaceted. Irradiation leads to the decomposition of water molecules, resulting in the formation of free radicals that can have both positive and negative effects on meat quality and storage. Although irradiation reduces the number of microorganisms and extends the shelf life of meat by damaging microbial DNA and cell membranes, it can also accelerate the oxidation of lipids and proteins, particularly sulfur-containing amino acids and unsaturated fatty acids. With regard to proteins, irradiation affects both myofibrillar and sarcoplasmic proteins. Myofibrillar proteins, such as actin and myosin, can undergo depolymerization and fragmentation, thereby altering protein solubility and structure. Sarcoplasmic proteins, including myoglobin, undergo structural changes that can alter meat color. Collagen, which is crucial for meat toughness, can undergo an increase in solubility owing to irradiation-induced degradation. The lipid content and composition are also influenced by irradiation, with unsaturated fatty acids being particularly vulnerable to oxidation. This process can lead to changes in the lipid quality and the production of off-odors. However, the effects of irradiation on lipid oxidation may vary depending on factors such as irradiation dose and packaging method. In summary, while irradiation can have beneficial effects, such as microbial reduction and shelf-life extension, it can also lead to changes in meat properties that need to be carefully managed to maintain quality and consumer acceptability.

Comparison between Siderophores Production by Fungi Isolated from Heavy Metals Polluted and Rhizosphere Soils

  • Hussein, Khalid A.;Joo, Jin Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.5
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    • pp.798-804
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    • 2012
  • Although siderophores are induced primarily in response to iron deficiency, soil and other ecological factors can affect on this process. This study was to evaluate the production of siderophores by different fungal species isolated from heavy metal contaminated and uncontaminated soils. More than thirty fungal strains were isolated from heavy metal contaminated and rhizosphere uncontaminated soils. Chrome azurol sulfonate (CAS) was used for both quantitative and qualitative evaluation of siderophores production. No significant correlations were observed between the tested variables such as ultraviolet (UV) irradiation method and CAS-agar plate and heavy metal concentration in both soils. The production of siderophores in rhizosphere fungi was higher than those isolated from the contaminated soil; however, the difference was not significant. The siderophore production (%) by fungi isolated from heavy metal contaminated soil using UV irradiation method was positively correlated with the qualitative values using CAS-plate method (P<0.05). Pearson correlation test indicated a positive correlation between the quantitative and qualitative methods of detection for fungi isolated from rhizosphere and also those isolated from heavy metal contaminated soil.

INHIBITORY EFFECT OF DENTAL LASERS ON THE GROWTH AND THE FUNCTION OF STREPTOCOCCUS MUTANS (각종 치과레이저의 Streptococcus mutans에 대한 증식 및 기능억제 효과)

  • Han, Kang-Seog;Kook, Joong-Ki;You, So-Young;Kim, Hwa-Sook;Park, Jong-Whi;Park, Heon-Dong;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.3
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    • pp.439-447
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    • 2003
  • This was performed to evaluate the inhibitory effect of laser on the growth of S. mutans. The bacterial pallets containing S. mutans KCTC 3065 were irradiated with Er:YAG laser and Nd :YAG laser by non-contact method at an intensity of 50mJ for 5 sec with the pulse repetition rates of 10Hz and 30Hz, respectively. The following results were obtained on colony count, acid producing ability, and the amount of insoluble extracellular polysaccharide synthesis. 1. The irradiation of Nd:YAG laser after photosensitization with Chinese ink inhibited the proliferation of S. mutans the most, and the irradiation of Er:YAG also inhibited the proliferation. However, the irradiation of Nd:YAG laser alone could not inhibited the proliferation of S. mutans. The pulse repetition rate did not affect significantly on the proliferation of bacteria in overall. 2. The irradiation of Nd:YAG laser after the photosensitization with Chinese ink inhibited the acid production of S. mutans the most for a certain period of time. Er:YAG laser also inhibited acid production. When Nd:YAG laser was used alone, the acid production of S. mutans was not been inhibited. The irradiation of Nd:YAG laser after photosensitization with Chinese ink inhibited the acid production ability of bacteria the most as the pulse repetition rate increased. 3. Laser irradiation did not inhibited the synthesis of insoluble extracellular polysaccharide of S. mutans. From these results, we conclude that the irradiation of Er:YAG laser and Nd:YAG laser after photosensitization with Chinese ink would inhibit the proliferation and acid production by S. mutans, which may prevent dental caries. However, this effect does not last long time so that the laser irradiation should be repeated frequently in order to obtain clinical effect; thus, this laser irradiation would not have a clinical usefulness in preventing dental caries when used solely.

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Weed and Pest Control by Means of Physical Treatments(I) -Effects of infrared irradiation on sandy loam for weed control- (물리적인 방법을 이용한 잡초 및 병해충방제 방법의 개발(I) -적외선 조사에 의한 잡초방제를 위한 사양토의 가열 효과-)

  • 강화석;유창연;신현동;강위수;오재헌
    • Journal of Biosystems Engineering
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    • v.21 no.1
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    • pp.21-33
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    • 1996
  • This study was to obtain basic information needed to develop the effective weed control method for the production of less polluted agricultural products by inducing viability loss of weed seeds in soil with infrared irradiation. Ceramic plates were heated by LPG with the aid of forced air and the infrared produced from plates was used as the heat source for heating soil. The soil heated in this study was sandy loam with four levels of moisture contents (0.5, 5.1, 9.1, 15.0% wb). The temperature distribution was measured at various soil depths when soil was irradiated with infrared for different irradiation time (30, 60, 90 sec). The soil depths with duration time of minimum 3 minutes over $80^circ C$, temperature inducing viability loss of weed seeds, were investigated. When the moisture content of soil was 0.5% and 5.1% wb, the soil depths which can induce viability loss of weed seeds was greatly increased with increasing irradiation time. When 30 seconds of irradiation time was applied on soil with moisture content of 9.1% or 15.0% wb, any depths of soil tested in this study was not reached to the temperature of 8$0^{\circ}C$. Generally, the soil depth being needed for viability loss of weed seeds was decreased with increasing moisture content of soil.

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Understanding Phytosanitary Irradiation Treatment of Pineapple Using Monte Carlo Simulation

  • Kim, Jongsoon;Kwon, Soon-Hong;Chung, Sung-Won;Kwon, Soon-Goo;Park, Jong-Min;Choi, Won-Sik
    • Journal of Biosystems Engineering
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    • v.38 no.2
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    • pp.87-94
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    • 2013
  • Purpose: Pineapple is now the third most important tropical fruit in world production after banana and citrus. Phytosanitary irradiation is recognized as a promising alternative treatment to chemical fumigation. However, most of the phytosanitary irradiation studies have dealt with physiochemical properties and its efficacy. Accurate dose calculation is crucial for ensuring proper process control in phytosanitary irradiation. The objective of this study was to optimize phytosanitary irradiation treatment of pineapple in various radiation sources using Monte Carlo simulation. Methods: 3-D geometry and component densities of the pineapple, extracted from CT scan data, were entered into a radiation transport Monte Carlo code (MCNP5) to obtain simulated dose distribution. Radiation energy used for simulation were 2 MeV (low-energy) and 10 MeV (high-energy) for electron beams, 1.25 MeV for gamma-rays, and 5 MeV for X-rays. Results: For low-energy electron beam simulation, electrons penetrated up to 0.75 cm from the pineapple skin, which is good for controlling insect eggs laid just below the fruit surface. For high-energy electron beam simulation, electrons penetrated up to 4.5 cm and the irradiation area occupied 60.2% of the whole area at single-side irradiation and 90.6% at double-side irradiation. For a single-side only gamma- and X-ray source simulation, the entire pineapple was irradiated and dose uniformity ratios (Dmax/Dmin) were 2.23 and 2.19, respectively. Even though both sources had all greater penetrating capability, the X-ray treatment is safer and the gamma-ray treatment is more widely used due to their availability. Conclusions: These results are invaluable for optimizing phytosanitary irradiation treatment planning of pineapple.

Comparison of Effects of Ultraviolet and $^{60}$ Co Gamma Ray Irradiation on Nylon 6 Mono-filaments

  • Ohtsuka, Mika;Suzuki, Yoshino;Sakai, Tetsuya;Netravali, Anil N.
    • Fibers and Polymers
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    • v.5 no.3
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    • pp.225-229
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    • 2004
  • The effect of UV and $^60{Co}$ gamma radiations on the physical and mechanical properties of nylon 6 mono-filaments with different draw ratios has been studied. Specimens were exposed to either up to 25 Mrad of gamma or up to 168 hrs of intense UV irradiation. The results show that nylon mono-filaments exposed to gamma rays, with much higher quantum energy than UV, undergo a larger extent of molecular chain scission. Higher irradiation dose also results in the production of insoluble, macroscopic three-dimensional cross-linked network structure. The amorphous regions with a lower density of cohesive energy (lower molecular orientation) show a higher extent of cross linking reaction whereas amorphous regions with a higher density of cohesive energy (higher orientation) show higher extent of chain scission reaction, irrespective of UV ray or gamma ray irradiation.