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Production of Haploids from Proton Ion and Gamma-Ray Irradiation Treated $M_2$ Generation of Isolated Microspores in Brassica napus L. ssp. oleifera  

Kim, Kwang-Soo (Mokpo Experiment Station, National Institute of Crop Science)
Li, Mei-Yang (Mokpo Experiment Station, National Institute of Crop Science)
Jang, Young-Seok (Mokpo Experiment Station, National Institute of Crop Science)
Park, Yoon-Jung (Mokpo Experiment Station, National Institute of Crop Science)
Bang, Jin-Ki (Mokpo Experiment Station, National Institute of Crop Science)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.53, no.2, 2008 , pp. 150-155 More about this Journal
Abstract
This experiment was carried out to investigate the effect of proton ion and gamma-ray irradiation on microspore culture of the flower buds of $M_2$ generation in winter type of Brassica napus L. ssp. oleifera. The seeds of three rape varieties, 'Halla', 'Naehan' and 'Tammi' were pretreated with proton ion and gamma-ray 400 Gy and 600 Gy, respectively. When microspore culture techniques were used, embryogenesis was increased in some varieties by proton ion and gamma-ray irradiation treated flower buds of $M_2$ generation than control. In genotypes 'Naehan' showed the highest embryo production frequency, but 'Tammi' showed lowest embryo production frequency. Some of the embryoids developed directly into plantlets, whereas others developed abnormally multilobe. Plants were regenerated and successfully acclimatized in pots.
Keywords
Brassica napus; mutagenesis; microspore culture; proton ion; gamma-ray irradiation;
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Times Cited By KSCI : 3  (Citation Analysis)
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