• Journal of the Korean Magnetic Resonance Society
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• v.22 no.2
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• pp.26-33
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• 2018

Xanthium sibiricum is used as a traditional folk medicine for the treatment of cancer, fever, headache, nasal sinusitis, and skin pruritus. This study aimed to identify components from Xanthium sibiricum extracts using an SPE-800MHz NMR-MS hyphenated system. The simultaneous acquisition of MS and NMR spectra from the same chromatographic peaks significantly increases the depth of information acquired for the compound and allows the elucidation of structures that would not be possible using MS or NMR data alone. LC -NMR analysis was conducted using a HPLC separation system coupled to 800 MHz spectrometer equipped with a cryoprobe, and a SPE unit was used to automatically trap chromatographic peaks using a HPLC pump. LC-MS analysis was conducted with a Q-TOF MS instrument using ESI ionization in the negative ion mode. Using the hyphenated analysis, several secondary metabolites were identified, such as 3',5'-O-dicaffeoylquinic acid, 1',5'-O-dicaffeoyl- quinic acid, and ethyl caffeate. These results demonstrate that the SPE-800MHz NMR-MS hyphenated system can be used to identify metabolites within natural products that have complex mixtures.

• Journal of Korean Society for Atmospheric Environment
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• v.13 no.6
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• pp.475-486
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• 1997

The characteristics of atmospheric aerosols were investigated as a function of particle size and water solubility. The atmospheric aerosols were sampled with classifying into 12 size ranges by the use of Andersen low-pressure impactor. Collected aerosol particles were extracted by ultrapure water and filtered to be separated into water-soluble and insoluble components. The concentrations 12 elements in both components were determined by PIXE analysis. And the concentrations of 8 ions in the soluble component were analyzed by ion chromatography. In general, the mass size distribution of particulate matter was represented as a bimodal distribution. The mass size distributions of S$(SO_4^{2-}), K(K^+), Zn and NH_4^+$ skewed to the smaller size range and those of Si, Ca$(Ca^{2+}), Fe, Na^+ and Mg^{2+}$ skewed to the larger size range. They had roughly one peak in the fine and coarse particle region,respectively. On the other hand, the mass size distribution of Ti, Mn, Ni, Cu, $Cl^- and NO_3^-$ were represented as the bimodal distribution. Fe and Si in the aerosol particles extracted into pure water are existing in high insoluble state. Conversely, almost the whole of S is dissolved in water.

• Journal of Environmental Health Sciences
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• v.24 no.3
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• pp.114-123
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• 1998

Particulate matters(less than 10 $\mu$m, PM10) at Kanghwa and Yangyang, which are located in the western and the eastern coastal regions in Korea, were measured in using low volume air sampler from December 1995 to November 1996, and their characteristics were investigated from the view point of background level.(and in order to characterize the particulate matters.) The particulate matters were analyzed for major water soluble ionic components(SO$_{4}^{2-}$, NO$_{3}^{-}$, Cl$^{-}$, Na$^{+}$, NH$_{4}^{+}$, K$^{+}$, Mg$^{2+}$ and Ca$^{2+}$) by ion chromatography. Mass concentrations of particulate matters were $48.77 \pm 22.45 \mug/m^{3}$ at Kanghwa and $54.04 \pm 32.98 \mug/m^{3}$ at Yangyang and SO$_{4}^{2-}$, NO$_{3}^{-}$ and NH$_{4}^{+}$ contributed largely to water soluble ionic components. nss(non sea salt)-SO$_{4}^{2-}$, contributed more than 95 percentage to SO$_{4}^{2-}$ and nss-K$^{+}$ and nss-Ca$^{2+}$ also contributed high percentages to K$^{+}$ and Ca$^{2+}$. It was supposed that most SO$_{4}^{2-}$, was originated from anthropogenic sources, and K$^{+}$ and Ca$^{+}$ were mainty originated from soil. The results of factor analysis suggested possibility of interpreting the correlation between air pollutants and regional characteristics.

• Natural Product Sciences
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• v.14 no.3
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• pp.152-155
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• 2008

An HPLC-ESI-MS method has been developed to identify and quantify two main tetracyclic triterpenes, alisol B 23-acetate and alisol C 23-acetate in the Alismatis Rhizoma (Taeg-Sa). The relative distribution of the two triterpenes in the methanolic extract of commercially available Alismatis Rhizoma was established by selective ion monitoring (SIM) mode via electrospray ionization (ESI) source. Regression equations revealed good linear relationship, and the correlation coefficients were 0.999 and 0.998 for alisol B 23-acetate and alisol C 23-acetate, respectively, between the peak areas of the components and their concentration in a range of $0.06-2.0{\mu}g/mL$. It was found that there were significant differences in the amount of alisol B 23-acetate and alisol C 23-acetate between Korean and Chinese origins. The results showed that this method could be used to identify the two components in Alismatis Rhizoma with high sensitivity and selectivity.

• Journal of Power System Engineering
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• v.4 no.3
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• pp.25-33
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• 2000

This study represents three-dimensional turbulent flow characteristics around an axial fan measured at the operating point ${\varphi}=0.32$, which is equivalent to the maximum flowrate region, by using three-dimensional fiber-optic type LDA system. This LDA system is composed of a 5 W Argon-ion laser, two optics in back-scatter mode, three BSA's, a PC, and a three-dimensional automatic traversing system. A kind of paraffin fog is used for laser particles in this study. Mean velocity profiles around an axial fan along the downstream radial distance show that the streamwise and the tangential components exist as a predominant velocity and have the maximum value at the radial distance ratio 0.8, while the radial component has a small scale distribution and its flow direction is inward except a part of blade tip. The turbulent intensity profiles show that the radial component exists the most greatly. And also the turbulent kinetic energy shows about 60% as a maximum value at the radial distance ratio 0.9. Moreover, the Reynolds shear stresses do not exist at upstream flow, but the streamwise and the radial components of them show about 20% as a maximum value at the radial distance ratio 0.9 at downstream flow.

• Asian Journal of Atmospheric Environment
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• v.4 no.2
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• pp.72-79
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• 2010

In this study, the ionic composition of volcanogenically derived particles and their temporal and spatial distributions have been investigated to evaluate the impact of the volcanic eruption on the local ecosystem and residents. To this end, an intensive field study was conducted to measure the size-segregated particulate matters at the east part of Sakurajima in Japan. Fractionated sampling of particles into > $PM_{10}$, $PM_{10-2.5}$, and $PM_{2.5}$ was made by a multi nozzle cascade impactor (MCI). The concentration of various ions present in the size-resolved particles was determined by Ion chromatography. The time dependent 3-dimensional Volcanic Ash Forecast Transport And Dispersion (VAFTAD) model developed by the NOAA Air Resources Laboratory (ARL) indicated that the sampling site of this work was affected by the volcanic aerosol particles plume. The temporal distributions of sulfate and $PM_{2.5}$ during the field campaign were significantly variable with important contributions to particle mass concentration. The chlorine loss, suspected to be caused by acidic components of volcanic gases, occurred predominantly in fine particles smaller than $10\;{\mu}m$.

• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.267-271
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• 2000

Abstract Two glucans (PONGa and PONGb) differing in their anomeric and glycosidic linkage structures were isolated from the water-insoluble materials (PON) of Pleurotus ostreatus basidiocarps, which activated the complement system and were almost soley composed of D-glucose. The isolatIon was achieved by repeated precipitations with ethanol and adsorption on concanavalin A (Con A) of paN suspension in thymol/NaCL Based on methylation analysis. IR, GLC-MS, $^1H,{\;}and{\;}^{13}C-NMR$ spectroscopies, PONGa was found to be a branched a-glucan composed of ${\alpha}-linked$ D-glucopyranose residues and ${\alpha}-linked$ units with 6-branching points, whereas PONGb was a linear ${\beta}-1,3-glucan$ composed mainly of ${\beta}-1,3-linked$ D-glucopyranose residues. The PONGb particles reacted more potently than the PONGa particles as C3 activator in alternative complement hemolysis and crossed-immunoelectrophoresis using anti-human C3, thereby suggesting that the complement activating components of PON were ${\beta}-(13)-glucans rather$ than ${\alpha}-glucan$ components.onents.

• Proceedings of the KSME Conference
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• 2001.11b
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• pp.371-376
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• 2001

The operating point of a small-sized axial fan for refrigerator is strongly dependent upon the system resistance. Therefore, the turbulent flow characteristics around a small-sized axial fan may change significantly according to the operating point. This study represents three-dimensional turbulent flow characteristics around a small-sized axial fan measured at the four operating points such as $\varphi=0.1$, 0.18, 0.25 and 0.32 by using fiber-optic type LDA system. This LDA system is composed of a 5 W Argon-ion laser, two optics in back-scatter mode, three BSA's, a PC, and a three-dimensional automatic traversing system. A kind of paraffin fluid is utilized for supplying particles by means of fog generator. Mean velocity profiles downstream of a small-sized axial fan along the radial distance show that both the streamwise and the tangential components exist predominantly in downstream except $\varphi=0.1$ and have a maximum value at the radial distance ratio of about 0.8, but the radial component, which its velocity is relatively small, is acting role that only turns flow direction to the outside or the central part of axial fan. Moreover, all of the velocity components downstream at $\varphi=0.1$ show much smaller than those upstream due to the static pressure rise at the low-flowrate region.

• Journal of Environmental Science International
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• v.12 no.3
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• pp.325-335
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• 2003

An agricultural land application of swine slurry is one of the best management practices in Jeju island whose ground water must be protected. So as to study the effect of appling swine slurry on ground water or aquifer, incubation-leaching technique was used by assuming the incubating period of 1, 2, 4, 8, 16, or 32 days, and application rate of 3200.0 mgT-N/$\ell$ , 820.0 mgT-P/$\ell$, and 1887.0 mgK$\^$+/$\ell$ in swine slurry. The leachates were collected from the soil columns(PVC 30 cm L${\times}$5.5 cm D) packed 15cm in depth with Gangjeong soil series by washing with 100 mL distilled water. The leached components were measured by using ion chromatography far Cl$\^$-/, NO$_3$-N, F$\^$-/, Br$\^$-/, Na$\^$+/, K$\^$+/, Ca$^2$$\^$+/, and Mg$^2$$\^$+/ , atomic absorption spectrophotometry for Fe and Mn, and UV-Vis spectrophotometry for T-N and T-p. Application of swine slurry in naked soil could influence on the ground water or aquifer by increasing nitrate-nitrogen in leachate with time, or leaching the cations present in soils in accompany with anions because of H$\^$+/produced in nitrification. Therefore, careful consideration should be taken about what amount, when, where, and how fur protecting ground water system.

• Journal of the Korean Vacuum Society
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• v.17 no.1
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• pp.1-8
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• 2008

We analyzed the characteristics of water leakage from cooling components of the storage ring in the Pohang Light Source. The water leaks led localized pressure bumps and abnormal pressure changes. The leakage also changed the residual gas compositions depending not only on the position between leakage place to gas analyzer but also on on/off switching of ion pump and electron beam. We found that the residual gas analysis of $CH_4$, CO, NO was useful in determining water leaks.