• Journal of Periodontal and Implant Science
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• v.26 no.4
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• pp.873-887
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• 1996

Platelet-derived growth factor(PDGF) is one of the polypeptide growth fators. PDGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. Recent studies indicated that demineralized root surface as the primary site for growth factor application has advantages over other application method, especially due to binding capacity of growth factor for exposed matrix component of deminera1ized dentin surface. The purpose of this study is to evaluate optimal application time of PDGF-BB on proliferation of human gingival fibroblasts using deminera1ized dentin surface as primary application site. Human gingival fibroblasts and dentin slabs were prepared from the first premolar tooth extracted for the orthodontic treatment, cells were cultured in DMEM/I0% FBS at the $37^{\circ}C$, 5% CO2 incubator. All of the dentin slabs were preconditioned with Tetracycline HCI(100mg/ml) solution and rinsed in PBS. In the cell proliferation experiment, experimental group was immersed in DMEM containing 10% FBS, 50ng/rnl PDGF-BB during different time(30sec, 1, 2, 4, 8 minutes) and dried. Cells at concentration of $1{\times}10^5$cells/ml were seeded in each culture well which contained dentin slabs and incubated for 6 hours. Then, all of the dentin slabs were moved into new 24 well culture dish and incubated for 24, 48, 72 hours. The cell counting was done by hemocytometer with inverted phase contrast microscope after trypsinization. The results were as follows : The application of PDGF-BB for 1, 2 min slightly increased the number of gingival fibroblasts, and the application of PDGF-BB for 4, 8 min prominently increased the number of gingival fibroblasts. The application of PDGF-BB for 4 min showed maximum proliferation rate of gingival fibroblasts at 24, 48, 72 hours, and the application of PDGF-BB for 8 min showed less proliferation rate of gingival fibroblasts compared to the application of PDGF-BB for 4 min at 24, 48, 72 hours. In conclusion, the application of PDGF-BB for 4 min appeared to be optimal to obtain maximum proliferation of gingival fibroblasts using demineralized dentin surface as primary applicaton site of PDGF-BB.

• Journal of the korean academy of Pediatric Dentistry
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• v.28 no.1
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• pp.72-81
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• 2001

Dendroaspis natriuretic peptide (DNP), recently isolated from the venom of the green Mamba snake Dendroaspis angusticeps, is a 38-amino acid peptide containing a 17-amino acid disulfide ring structure similar to that of the natriuretic peptide family. The natriuretic peptide family was known to induce histamine release from human and rat mast cells, but there are no published data concerning the effects of DNP on histamine release from mast cells. The purpose of this study is to investigate whether DNP induces the histamine release from rat peritoneal mast cells (RMPCs) and to determine the mechanism of DNP-induced histamine release from RPMCs. After treatment of the various doses of DNP in RPMCs, the mast cell degranulation was observed with inverted microscopy and the histamine release was measured by radio-enzymatic assay. Calcium uptake and intracellular cyclic GMP level were measured by radioimmunoassays. DNP induced the mast cell degranulation. DNP released the histamine and increased the calcium uptake and the level of intracellular cyclic GMP of RPMCs, in a dose-dependent manner. The results indicate that DNP is capable of inducing histamine release from RPMCs by increasing of calcium uptake and intracellular cyclic GMP level.

• Korean Journal of Social Welfare
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• v.68 no.4
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• pp.53-74
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• 2016

In this study, to verify influence that economic situations of a household unit have on donation behavior, after economic situations of a household unit were measured with income and assets by type and income to assets, effect which each variable has on secular giving was verified. This study used 3-year panel data of a total of 4,938 households based on the fifth to seventh year data as investigation data from 2012 to 2014 among data of financial panel investigation of Korea Institute of Public Finance of National Survey of Tax and Benefit. As an analysis method, a random effect tobit model was used. At the analysis result, it appeared that as scales of financial assets, earned income, property income, and transfer income become larger, the amount of donation increased. Also, it was represented that rates of income to assets had negative influence on secular giving. In case of demographic variables, education levels of householders and sizes of houses had relation of a positive direction. In case of ages, it appeared that they had inverted U-type relation. This study has meanings in that understanding of donation behavior of a household unit can be increased and expanded more and also implications related to intervention strategies of a household unit to expand sharing culture can be extracted by verifying influence which economic factors of a household unit have on donation behavior based on panel data.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7229-7234
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• 2014

Trichoderma spp. are known as a rich source of secondary metabolites with biological activity belonging to a variety of classes of chemical compounds. These fungi also are well known for their ability to produce a wide range of antibiotic substances and to parasitize other fungi. In search for new substances, which might act as anticancer agents, the overall objective of this study was to investigate the cytotoxic effects of Trichoderma harzianum and Trichoderma asperellum cultural filtrates against human cervical and breast cancer cell lines (HeLa and MCF-7 cells respectively). To achieve this objective, cells were exposed to 20, 40, 60, 80 and 100 mg/ml of both T. harzianum cultural filtrate (ThCF) and T. asperellum cultural filtrate (TaCF) for 24h, then the cell viability and the cytotoxic responses were assessed by using trypan blue and 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assays. Morphological changes in cells were investigated by phase contrast inverted microscopy. The results showed that ThCF and TaCF significantly reduce the cell viability, have cytotoxic effects and alter the cellular morphology of HeLa and MCF-7 cells in a concentration dependent manner. A concentration of 80 and 100mg/ml of ThCF resulted in a sharp decline in the cell viability percent of HeLa and MCF-7 respectively (25.2%, 26.5%) which was recorded by trypan blue assay. The half-maximal inhibitory concentrations ($IC_{50}$) of ThCF and TaCF in HeLa and MCF-7 were recorded as 16.6, 12.0, 19.6 and 0.70mg/ml respectively by MTT assay. These results revealed that ThCF and TaCF have a substantial ability to reduce the viability and proliferation of human cervical and breast cancer cells.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.37 no.11
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• pp.8-16
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• 2000

In this paper, on calculating SARs on human head using computer simulation, SARs caused by PCS handsets are calculated and compared, and the design parameters that affect SAR values are analyzed. Is and 10 g peak averaged SARs are calculated as the type of antenna, the location of antenna, and the type of handset are changed and SAR distributions as depth of human head are shown. Among the antennas on flip type handsets, side mounted PIFA has the lowest SARs 1g and 10g peak averaged SARs are 0.686W/kg and 0.353W/kg. The SARs caused by monopole antenna on folder type handset are 1.133W/kg and 0.709W/kg. and are about 30% lower than monopole 1.759W/kg and 0.978W/kg, respectively. SAR distributions as depth of human head of side mounted PIFA and monopole antenna on folder-type handset are more slowly changed than those of top mounted PIFA and monopole antenna on flip-type handset.

• Clinical and Experimental Reproductive Medicine
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• v.29 no.2
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• pp.139-147
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• 2002

Objective : This study was to establish a reproducible differentiation system from the parthenogenetic mouse embryonic stem (P-mES02) cells into functional cardiomyocytes like as in vitro fertilization mouse embryonic stem (mES01) cells. Materials and Methods: To induce differentiation, P-mES02 cells were dissociated and aggregated in suspension culture environment for embryoid body (EB) formation. For differentiation into cardiomyocytes, day 4 EBs were treated with 0.75% dimethyl sulfoxide (DMSO) for another 4 days (4-/4+) and then were plated onto gelatin-coated dish. Cultured cells were observed daily using an inverted light microscope to determine the day of contraction onset and total duration of continuous contractile activity for each contracting focus. This frequency was compared with the results of DMSO not treated P-mES02 group (4-/4-) and mES01 groups (4-/4+ or 4-/4-). For confirm the generation of cardiomyocytes, beating cell masses were treated with trypsin-EDTA, dispersed cells were plated onto glass coverslips and incubated for 48 h. Attached cells were fixed using 4% paraformaldehyde and incubated with specific antibodies (Abs) to detect cardiomyocytes (anti-sarcomeric ? -actinin Ab, 1 : 100; anti-cardiac troponin I Ab, 1 : 2000) for 1 h. And the cells were finally treated with FITC or TRITC labelled 2nd Abs, respectively, then they were examined under fluorescence microscopy. Results: Rhythmically contracting areas in mES01 or P-mES02 cells were firstly appeared at 9 or 10 days after EBs plating, respectively. The highest cumulative frequency of beating EBs was not different in both treatment groups (mES01 and P-mES02, 4-/4+) with the results of 61.3 % at 13 days and 69.8% at 15 days, respectively. Also, the contracting duration of individual beating EBs was different from minimal 7 days to maximal 53 days. However, DMSO not treated groups (mES01 and P-mES02, 4-/4-) also had contracting characteristics although their frequency was a few compared to those of DMSO treated groups (6.0% and 4.0%). Cells recovered from the spontaneously contracting areas within EBs in both treated groups were stained positively with muscle specific anti-sarcomeric ? -actinin Ab and cardiac specific anti-cardiac troponin I Ab. Conclusion: This study demonstrated that the P-mES02 cell-derived cardiomyocytes displayed similarly structural properties to mES01 cell-derived cardiomyocytes and that the DMSO treatment enhanced the cardiomyocytes differentiation in vitro.

• 한국지구물리탐사학회:학술대회논문집
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• 2006.06a
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• pp.125-130
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• 2006

Both surface seismic and far-offset VSP data were recorded alongtwo mutually perpendicular profiles in the Pungam basin. The first-arrival times were simultaneously inverted using the tomography method. For the surface data, seismic energy was generated by a 5-kg sledgehammer at 48 stations and detected by 21 surface geophones at 3 m intervals and one 3-component geophone in test borehole for the purpose of static corrections. For the VSP data, seismic waves generated by the sledgehammer on the ground were detected by a 3-component borehole geophone in a depth range of $9{\sim}99\;m$. Delay times of the hammer data were corrected using the seisgun data before the inversion to yield velocity tomograms. The tomograms indicates that the soil layer with velocities less than 750 m/s averages 1.8 m thick. The velocity varies from 5353 m/s at the depth range of $31{\sim}40\;m$ to 4262 m/s at the depth range of $65{\sim}73\;m$. Compared with core samples, the relatively large variation in velocity may due to lithology changes and fracture effects with depth.

• Geophysics and Geophysical Exploration
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• v.20 no.3
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• pp.163-175
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• 2017

In land seismic exploration, irregular surface topography and weathering layer in near surface distorts the reflected signals of data. Therefore, typical land seismic data should be compensated for this distortion by static correction. To perform the static correction, near-surface velocity is required, which can be obtained by seismic refraction survey. However, land seismic data is often acquired in a limited form of geometry depending on the equipment availability, accessibility condition, and permission for the survey site. In this situation, refraction analysis should be performed using reflection data because it is impossible to acquire refraction-oriented data due to limited source and receiver geometry. In this study, we aimed to analyze the reliability of the results obtained by refraction traveltime tomography when using reflection data with a limited number of sources and receivers from irregular surface topography. By comparing the inversion result from irregular topography with that from flat surface, we found that the surface topography affects the reliability of the inversion results to some degree. We also found that the number of sources has little effect on the inversion results unless the number of sources are very small. On the other hand, we observed that velocity distortion occurred in the overlapped part of receiver arrays when using a limited number of receivers, and therefore suggested the size of the least overlapping ratio to avoid the velocity distortion. Finally, we performed numerical tests for the model which simulates the surface topography and acquisition geometry of the survey region and verified the reliability analysis of inversion results. We identified reliable areas and suspicious area of the inverted velocity model by applying the analysis results to field data.

• Geophysics and Geophysical Exploration
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• v.21 no.1
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• pp.33-40
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• 2018

We investigate the crustal and uppermost mantle SV- and SH-wave velocity structure and radial anisotropy beneath East Asia including Korea, China and Japan. Rayleigh waves and Love waves were extracted from the seismic data recorded at broadband seismic stations in East Asia. Using the MFT (Multiple Filter Technique), we obtained group velocity dispersion curves of Rayleigh and Love waves with a period range of 3 to 200 s. We obtained 62466 Rayleigh-waves dispersion-curve measurements in vertical components and 54141 Love-waves dispersion-curve measurements in transverse components, respectively. The inverted models using these data sets provide SV- and SH-wave velocity structure of crust and uppermost mantle down to 100 km depth. In both cases of the S-wave velocity structures, strong high-velocity anomalies are observed down to 30 km depth beneath the East Sea, and deeper than 30 km depth, strong low-velocity anomalies are found beneath the Tibetan plateau. In the case of the SH-wave velocity structure, strong low-velocity anomalies are observed beneath the East Sea deeper than 30 km depth, leading to negative anisotropy. On the other hand, positive anisotropy is usually observed beneath the Tibetan plateau.

• Archives of Plastic Surgery
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• v.33 no.2
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• pp.205-212
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• 2006

Using adipose derived stem cells(ASCs), neurogenic differentiation was induced in a mono layered culture medium containing neuronal induction agents. Cells differentiated to the neuronal cells were observed with a inverted microscope and immunofluorecent study. We made a 15 mm long defect in the sciatic nerve of 14 rats and connected a silicone tube to the defect. Then, we mixed neuronal progenitor cells differentiated from ASCs with collagen gel and grafted them to a group of rats(experimental group) and grafted only collagen gel into another group(control group). In 4 and 8 weeks after the graft, histological observation was made. According to the result, the number and diameter of myelinated axons were significantly increased in the experimental group. In addition, the nerve conduction velocity was improved more in the experimental group and neovascularity also increased. Moreover, reaction with S100 and p75 was observed in regenerated nerves in the experimental group, suggesting that the grafted cells were differentiated into supportive cells such as Schwann's cells. In conclusion, this research proved that ASCs can multiply and differentiate into neuronal cells. If they are grafted into nerve defects, the grafted cells are differ entiated into supportive cells such as Schwann's cells and thus contribute to nerve regeneration. Accordingly, the use of adipose tissue obtained easily without the limitation of donor site can be greatly helpful in treating peripheral nerve defects.