• 제목/요약/키워드: Intraspecific diversity

검색결과 77건 처리시간 0.031초

Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences

  • Liu, Zhigao;Korpelainen, Helena
    • Horticulture, Environment, and Biotechnology : HEB
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    • 제59권6호
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    • pp.889-897
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    • 2018
  • Currently, there is a lack of genetic markers capable of effectively detecting polymorphisms in Clematis. Therefore, we developed new markers to investigate inter- and intraspecific diversity in Clematis. Based on the complete chloroplast genome of Clematis terniflora, simple sequence repeats were explored and primer pairs were designed for all ten adequate repeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS region was sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markers that can distinguish different species and be used in different genetic analyses, including cultivar identification to assist the breeding of new ornamental cultivars.

DNA Barcoding of the Endangered Species Ellobium chinense (Mollusca, Gastropoda, Ellobiidae) from Coastal Areas of South Korea

  • Yi, Chang Ho;Jung, Tae Won;Kim, Il-Hun;Cho, In-Young;Kim, Min-Seop;Yoon, Moongeun;Kim, Won
    • Animal Systematics, Evolution and Diversity
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    • 제35권3호
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    • pp.136-139
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    • 2019
  • The pulmonate gastropod Ellobium chinense (Pfeiffer, 1864) is an endangered marine species along the South Korean coasts due to habitat destruction and population declines. We sequenced the cytochrome c oxidase subunit 1 (COI) of 25 E. chinense specimens collected from five coastal sites in South Korea, and identified 16 unique haplotypes. The maximum intraspecific variation among individuals was 1.6%, while interspecific differences from another ellobiid species, Auriculastra duplicata (Pfeiffer, 1854), ranged from 21.9 to 23.0%. Our barcoding data will be useful to elucidate the phylogenetic relationships among pulmonate gastropods and infer the population genetic structure of E. chinense.

DNA Barcoding of Antarctic Freshwater Copepod Boeckella poppei (Crustacea: Copepoda: Calanoida: Centropagidae) Inhabiting King George Island, South Shetland Islands, Antarctica

  • Kang, Seunghyun;Jo, Euna
    • Animal Systematics, Evolution and Diversity
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    • 제36권4호
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    • pp.396-399
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    • 2020
  • The Antarctic freshwater copepod, Boeckella poppei (Mrazek, 1901), has the widest range of distribution extending from southern South America to Antarctic continent, among all Boeckella species. Boeckella poppei is the only freshwater copepod known to be inhabiting the Antarctic continent. In present study, we analyzed the DNA barcodes of the mitochondrial cytochrome c oxidase subunit I (COI) gene of B. poppei from King George Island, Antarctica. The intraspecific genetic distances varied from 0% to 13% and interspecific genetic distances ranged from 11% to 14%. The overlap of DNA barcode gap suggests careful threshold-based delimitation of species boundaries.

DNA Barcoding of Scolelepis (Scolelepis) sagittaria (Annelida, Spionidae) in Korea, with a Morphological Variability of the Species

  • Lee, Geon Hyeok;Yoon, Seong Myeong;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • 제38권3호
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    • pp.144-147
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    • 2022
  • The polychaete Scolelepis (Scolelepis) sagittaria was originally described from Japanese waters and subsequently reported from Korean waters. In this study, we determined for the first time the mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA (16S rDNA), and nuclear 18S ribosomal DNA (18S rDNA) sequences of Korean specimens of S. (S.) sagittaria. We also assessed intraspecific variation in the shape of the prostomium of this species based on an examination of 247 individuals. All materials were collected from intertidal sandy beaches of the Korea Strait. The molecular data and morphological observations reported herein will contribute to gaining a better understanding of the taxonomic relationships among members of the genus Scolelepis.

A New Record of Solitary Coral, Paracyathus rotundatus (Anthozoa: Scleractinia: Caryophylliidae), from Korea

  • Su-Hwan Sim;Hyo-Jin Yu;Sang-Hoon Park;In-Young Cho;Won-Gi Min;Sung-Jin Hwang
    • Animal Systematics, Evolution and Diversity
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    • 제39권2호
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    • pp.61-66
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    • 2023
  • Through a taxonomic study on solitary corals collected from subtidal zones of South and East Seas from 2019 to 2022, Paracyathus rotundatus, an unrecorded species belonging to family Caryophylliidae, is newly added to the anthozoan fauna of Korea. In addition, comparing partial 16S rRNA sequences with a length of 269 bp according to color variation clarified that there were no sequence differences among specimens with color variation, indicating that the color variation is due to intraspecific variation. As a result of this study, a total of 7 genera and 8 species belonging to the Caryophylliidae family have been reported in Korean waters so far.

A New Species of Antennella (Hydrozoa: Leptothecata: Halopterididae) from Korea

  • Hyo-Jin Yu;Seung-Joon Lee;Sung-Jin Hwang
    • Animal Systematics, Evolution and Diversity
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    • 제40권1호
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    • pp.79-87
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    • 2024
  • A taxonomic examination of hydroids collected from the subtidal zones of Ulleungdo and Dokdo located in the East Sea of Korea from 2017 to 2023 was conducted. Through this study, Antennella aurantia n. sp., a new species belonging to the family Halopterididae, was newly added to the hydrozoan fauna of Korea. This new species is clearly distinguished from similar species by the different morphology and size of the hydrothecae, lateral nematothecae, and gonothecae. By comparing the 16S rRNA sequence with 7 recorded species within the Antennella, the interspecific genetic distance to the new species was 8.2-28.9%, which is very high compared to the intraspecific variation of 0.0%, showing that it was clearly distinguished from other species. As a result of this study, a total of 4 Antennella species are reported in the hydrozoan fauna of Korea.

濟州 地域에서 개망초의 發芽 習性 및 耐陰性과 群落特性 (Germanition, Shade Toarance and Community Characteristics on $\emph{Erigeron annuus}$ L.in Cheju)

  • Lee, Ho-Joon;Kim, Tae-Sung;Byun, Doo-Weon
    • The Korean Journal of Ecology
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    • 제15권2호
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    • pp.103-115
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    • 1992
  • This dissertation, which has been prepared in the region of cheju(33o31'N, 126o32'E) from may,1987 to appril, 1989, is to elucidate the dominant characteristics of early stages in secondary succession, by examining the life history of erigeron annuus for the seeding depth of a seed was dependent upon the degree of shading. That is, on the area of ocm,the more tense the shading was, the more germination percentage was increased, while, in the case of 1.5cm, it was somewhat increased, when shading bwcame ess and less tense. The rosette of e. annuus, through its relative light intensity was decreased by shading, up to 5% of natural light, showed some strong shade tolerance, which had no difficulty in matter production. The species diversity of mixed-species community was the highest in the middle of April, the lowest in the last of may. The dominance index was the highest in the late may, the lowest in the middle of April. Toward the end of may, the occupation rate of dominance species was the highest. There was a neutral relationship between intraspecific and interspecific, owing to the differentiation of its niche, temporally and spatially. Productive structure of the community revealed a narrow leaf type which was concentratively distributed in the mid part of community height. The relative light intensity of community ground surface was 6.1%, the leaf inclination 60o,the extinction coefficient(k) 0.4, biomass of community 1,045.6 g.d.w./m2,T/Rratio 9.3, C/Frate 7.0 and sumgermanition, shade toarance and community characteristics on erigeron annuus l.in chejumed leaf area index 3.88. through the various life cycles o e.annuus, we can say that it decreases mortality of seeding caused by some stress and disturbance, for germination lasts for a long time at any opportunity available, and it promotes population growth. The strong shade tolerance of a rosette and the variableness of a life from differentiate the niche between intraspecific and interspecific within the community, and avoid the direct competition between them, thus poromoting community growth.

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가시오갈피 수집종의 RAPD 변이분석 (Intraspecific Relationship of Eleutherococcus senticosus Max. by RAPD Markers)

  • 김선;김기영;박문수;최선영;윤성중
    • 한국약용작물학회지
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    • 제6권3호
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    • pp.165-169
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    • 1998
  • 가시오갈피의 지역 수집 종간 변이의 정도와 유연 관계를 검토하고자 덕유산, 오대산, 북해도 수집종을 공시하여 RAPD를 실시 한 결과 가시 오갈피 유연관계 분석에 적합한 Primer로 UBC Primer $#200{\sim}300$ #208등 20개의 primer를 선발하였고, 다형화율은 $7.1{\sim}90.9%$를 나타냈으며, 총 증폭된 band 157개 중 113개 의 다형 band를 얻을 수 있었다. 가시오갈피 수집종은 2그룹으로 분류되었으며, 1그룹으로 분류된 덕유산 1, 2, 3, 4, 6, 북해도 7, 8, 오대산 9, 10번 개체내의 유사도는 $0.65{\sim}0.86$으로 나타났으나, 변종으로 추정되는 덕유산 수집종 5번 개체는 다른 수집 종들과의 유사도는 0.27로 극히 낮았다. 또한 동일지역 수집종간에 유사 값은 다른 지역에서 수집된 개체들과 차이가 없었고, 화사길이에 따른 차이도 구분되지 않았다.

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RAPD 분석에 의한 가시오갈피의 유연관계 분석 (Intraspecific Relationship Analysis of Eleutherococcus senticosus Max. by RAPD Markers)

  • 임정대;성은수;최강준;김승경;김명조;유창연
    • 한국자원식물학회지
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    • 제13권2호
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    • pp.104-110
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    • 2000
  • 가시오갈피 및 오갈피의 수집종 간의 유연관계를 구명하기 위하여 RAPD 분석을 한 결과 10개의 primer를 선발하였으며 G+C의 수가 모두 60%이상이었다. 10개의 primer를 사용하여 얻을 수 있는 총 밴드 수는 106개 였으며 이중 monomorphic한 밴드는 17.9%에 해당하는 19개였으며 나머지 87개는 polymorphic한 것으로 나타났다. 10개의 primer를 사용하여 얻은 106개의 밴드를 각각 하나의 형질(character)로 보아 이를 유연관계를 분석한 결과 영월 수집종과 일본종 및 지리산 오갈피와 서울오갈피를 포함하는 군(Group I )과 국내종과 러시아종, 중국종을 포함하는 군(Group II)으로 나뉘어졌으며 genetic distance값의 평균은 0.61이었다. Group I으로 분류된 북해도 가시오갈피는 국내의 각 수집지역의 가시오갈피나 러시아 가시오갈피와 원연의 관계인 것으로 나타났으며 2군에 포함된 수집 지역종 간의 원연 관계 중 춘천 수집종은 국내의 다른 지역인 잠곡이나 태기산 오대산 등과 비교하여 러시아 산이나 중국산에 대하여 더 근연의 관계를 나타내었다.

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분자표지를 이용한 마(Dioscorea spp.)의 다양성 분석 (Genetic Diversity among Dioscorea, spp. Using Molecular Markers)

  • 장광진;최익영;박주현;박종인;윤병성;김남수
    • 현장농수산연구지
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    • 제4권1호
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    • pp.45-54
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    • 2002
  • 마(Dioscorea spp.) 계통 중 품질이 우수하고 이용 가치가 기대되는 51개 계통에 대한 특성을 조사하고 도입재배 가능성을 시험했다. 또한 종간, 계통 간을 AFLP 분석하였고 생육 패턴을 시험하였다. 1. AFLP분석의 분자마커를 이용한 도입마의 유사성 분석에서 62% 수준에서 도입마는 다른 종간에 유의성이 나타났고 주성분 분석에서도 구분이 가능하였다. 그 중 D. alata는 일부 수집 지역별로 그룹이 형성되었다. 2. 일본의 쿠마모도(Kumamoto)지방에서 수집된 D. alata의 계통들의 유사성이 높은 것으로 보아, 그 지역의 마 유전자원의 이동이 적은 것으로 보인다. 반면, Indonesia, palau Is., 파푸아뉴기니아에서의 수집종들의 유연성이 높은 것은 한 지역의 계통이 여러지역으로 이동된 것으로 사료되다. 3. D. nipponica에 속하는 부채마는 다른 종과 변이가 많은 것으로 조사되어 유전적 가치가 높은 것으로 인정된다.