• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.130-130
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• 2003

Gummy stem blight pathogen is very difficult not only to monitor the inoculum levels prior to host infection, and also it is destructive and hard to control in field condition. We have applied RAPD technique to elucidate the genetic diversity of the genomic DNA of Didymella bryoniae and also to generate specific diagnostic DNA probe useful for identification and detection. The 40 primers produced clear bands consistently from the genomic DNA of twenty isolates of Didymella bryoniae, and two hundred seventy-three amplified fragments were produced with 40 primers. The combined data from 273 bands was analyzed by a cluster analysis using UPGMA method with an arithmetic average program of NTSYS-PC (Version 1.80) to generate a dendrogram. At the distance level of 0.7, two major RAPD groups were differentiated among 20 strains. RAPD group (RG) I included 8 isolates from watermelon except one isolate from melon. RAPD group (RG) IV included 12 isolates from squash, cucumber, watermelon and melon.. In amplification experiment with SCAR specific primer RG1F-RG1R resulted in a single band of 650bp fragment only for 8 isolates out of 20 isolates that should be designated as RAPD Group 1. However, same set of experiment done with RGIIF-RGIIR did not result in any amplified product.. Our attempts to detect intraspecific diversity of ITS region of rDNA by amplifying ITS region and 17s rDNA region for 20 isolates and restriction digestion of amplified fragment with 12 enzymes did not reveal polymorphic band. In order to develop RAPD markers for RGIV specific primer, a candidate PCR fragment( ≒1.4kb) was purified and Southern hybridized to the amplified fragment RGIV isolates. This promising candidate probe recognized only RGIV isolates

• ALGAE
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• 제33권4호
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• pp.291-304
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• 2018

Lyngbya C. Agardh ex Gomont is a nonheterocytous cyanobacterial genus whose evolutionary history is still poorly known. The traditionally defined Lyngbya has been demonstrated to be polyphyletic, including at least five distinct clades, some of which have been proposed as new genera. Intraspecific diversity is also clearly underestimated in Lyngbya due to the lack of unique morphological characters to differentiate species. In this study, we describe the new genus Capillus T. A. Caires, C. L. Sant'Anna et J. M. C. Nunes from benthic marine environments, including two new Brazilian species (here described as C. salinus T. A. Caires, C. L. Sant'Anna et J. M. C. Nunes, and C. tropicalis T. A. Caires, C. L. Sant'Anna et J. M. C. Nunes), and two species yet to be described, one of them from India (Capillus sp. 2.1), and the other from United States of America, based on strain PCC 7419. Capillus species presented cross-wise diagonal fragmentation, assisted or not by necridic cells, which has not been previously mentioned for Lyngbya. Ultrastructural analyses showed that C. salinus and C. tropicalis have numerous gas vesicles, which are rarely described for benthic marine species. The new genus formed a well-supported clade, and the D1-D1' and Box B secondary structures of internal transcribed spacer also supported the proposal of its new species. These findings help to clarify the diversity of species in the Lyngbya complex and the taxonomy of the group, and highlight the need of further floristic surveys in tropical coastal environments, which remain poorly studied.

• 한국잡초학회지
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• 제8권3호
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• pp.317-323
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• 1988

본(本) 연구(硏究)는 시설재배(施設栽培)에서 잡초방제(雜草防除)의 기초자료(基礎資料)를 얻고자 대전시(大田市) 유성지역(儒城地域)에서 1985년(年) 2월(月)부터 6월(月)까지 15일(日) 간격(間隔)으로 여러 시설재배조건(施設栽培條件)에서 잡초수(雜草數), 건물중(乾物重), 발생잡초(發生雜草)의 종생태적(種生態的) 및 사회적(社會的) 특성분석(特性分析)과 우점도(優占度)를 조사(調査)하였다. 1. 시설재배(施設栽培)에서 노지(露地)의 경우, 명아주, 여뀌, 쇠비름, 바랭이가 우점(優占)하였으며, PE 하우스는 냉이, 명아주, 바랭이, 쇠비름이 우점(優占)하였고 발생잡초(發生雜草) 본수(本數)는 무멀칭에 비해 멀칭과 턴넬내 멀칭에서는 많이 발생(發生)했으며 건물중(乾物重)은 이와는 반대로 무멀칭에서 무거웠다. 노지(露地)에서는 여름 광엽잡초(廣葉雜草)와 여름 화본과잡초(禾本科雜草)가 많이 발생(發生)했으나 PE 하우스내에서는 무멀칭에서 월동광엽잡초(越冬廣葉雜草)가 우점(優占)했으며 멀칭에서는 초기(初期)에 월동화본과잡초(越冬禾本科雜草)와 월동광엽잡초(越冬廣葉雜草)가 많이 발생(發生)했으나 후기(後期)에는 여름 화본과잡초(禾本科雜草)와 여름 광엽잡초(廣葉雜草)가 많이 발생(發生)하였다. 2. 시설내(施設內)에서 PE 하우스는 시설(施設)을 하므로 Simpson 지수(指數)가 높아 몇 초종(草種)이 우점(優占)하고 있는 군락(群落)을 이루었고 종다양도(種多樣度), 최대종다양도(最大種多樣度)가 안정도(安定度)에 비해 낮았고 또한 무피복보다 멀칭, 턴넬내 멀칭에서 높았으며 노지(露地)는 종다양도(種多樣度), 최대종다양도(最大種多樣度)가 멀칭, 턴넬을 하므로써 높아졌고 Simpson지수(指數)는 무멀칭에서 높고 오히려 시설(施設)을 하므로 낮아졌으며, PE 하우스, 노지(露地) 공(共)히 종간경쟁(種間競爭)이 종내경쟁(種內競爭)보다 높았던 것으로 생각된다.

• 한국자원식물학회지
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• 제23권6호
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• pp.541-549
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• 2010

The present study researched morphological characteristics and analyzed the genetic diversity by using RAPD in Calanthe species, native plant in Jeju, Korea. Twenty-six samples were selected by flower color, and 19 horticultural traits were investigated to study morphological characteristics. The C. discolor had the smallest leaf, the length and width of dorsal sepal, lateral sepal, petal, central lip, lateral lip, and flower stalk length were shortest and/or smallest except the spur and ovary length in Calanthe species, but those of Calanthe discolor for. sieboldii (Dence.) Ohwi (Calanthe discolor for. sieboldii) were the largest and/or biggest, and those of variants were the intermediate between C. discolor and C. discolor for. sieboldii, but spur length was the longest in C. discolor, the shortest in C. discolor for. sieboldii, and intermediate in the variants. Ovary length in C. discolor was shortest and C. discolor for. sieboldii and variants were similar with each other. The flower colors of C. discolor were brownish red, the value of CIE Lab was between 40 and 50. The flower color of C. discolor for. sieboldii was yellowish; the value of CIE Lab was between 110 and 130. And variants had various colors between 50 and 70 in the value of CIE Lab. After analyzing multiple band patterns of PCR products, 154 bands were selected as polymorphic RAPD markers. The analysis of Genetic distance of Calanthe species using RAPD showed that C. discolor and C. discolor for. sieboldii are more distant from each other than variants, and demonstrated the fact that genetic position of variants is between the other two species.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.78-83
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• 1999

Thirty-six environmental isolates of Vibrio vulnificus obtained from seawater, sediments, and raw seafoods, and 18 clinical isolates from Vibrio septicemia patients were typed by restriction endonuclease digestion profiles (REDP) of genomic DNA with SfiI. The results revealed a high-level of variation in REDPs, indicating a vast genomic diversity among V. vulnificus strains. Genetic relatedness of the strains showed similarities ranging from 10％ to 100％. Different REDPs for isolates from various raw seafoods were obtained, and clustering of strains according to type of seafoods was not observed. In contrast, clinical isolates of V. vulnificus showed higher similarity to one another, and could be subdivided into one separate group. The difference in REDPs of the V. vulnificus isolates from clinical origin and from raw seafoods substantiates the previous observation that only a single type of pathogenic strain was involved in each human infection, despite the numerous genetically polymorphic strains found from implicated oysters.

• Animal Systematics, Evolution and Diversity
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• 제21권1호
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• pp.93-110
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• 2005

한국 담수산 검물벼룩류에 대한 분류학적 연구의 일환으로, 보통검물벼룩(Mesocyclops)속의 5종을 분류하였다: 보통검물벼룩 (M. leuckarti) 갈고리보통 검물벼룩 (M. pehpeiensis), 꼭지보통검물벼룩 (M. dissimilis), 민가시보통검물벼룩 (M. woutersi), 털줄보통검물벼룩 (M. mariae). 이 중 뒤의 3종은 한국미기록 종이다. 이들 5종의 분류학적 미세 형질과 종내 변이성을 검토하였다. 한국산 보통검물벼룩속의 종 검색표를 작성하였다.

• Animal Systematics, Evolution and Diversity
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• 제21권2호
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• pp.137-156
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• 2005

한국 담수산 검물벼룩류에 대한 분류학적 연구의 일환으로, 톱니꼬리검물벼룩(Eucyclops serrulatus) 복합군에 속하는 6종을 보고한다: 톱니꼬리검물벼룩 (E. serrulatus), 예쁜톱니꼬리검물벼룩(E. roseus), 큰가시톱니꼬리검물벼룩(E. speratus), 태평톱니꼬리검물벼룩(E. Pacificus), 대머리톱니꼬리검물벼룩(E. ohtakai), 대마톱니꼬리검물벼룩(E. tsushimensis). 한국에서는 지금까지 다수의 보고서와 논문을 통해 톱니꼬리검물벼룩(E. serrulatus) 1종으로 기록되어 있었으나, 본 연구를 통해 이것이 6종의 자매종으로 구성된 복합군임을 확인하였다. 자매종간 분류학적 미세 형질을 서로 비교하였고, 종내 변이성에 대하여도 고찰하였다. 현재까지 극동아시아에서 기록된 톱니꼬리검물벼룩 복합군의 종 검색표를 작성하였다.

• Animal Systematics, Evolution and Diversity
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• 제27권2호
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• pp.159-163
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• 2011

DNA barcoding of Bryozoa or "moss animals" has hardly advanced and lacks reference sequences for correct species identification. To date only a small number of cytochrome c oxidase subunit I (COI) sequences from 82 bryozoan species have been deposited in the National Center for Biotechnology Information (NCBI) GenBank and Barcode of Life Data Systems (BOLD). We here report COI data from 53 individual samples of 29 bryozoan species collected from Korean seawater. To our knowledge this is the single largest gathering of COI barcode data of bryozoans to date. The average genetic divergence was estimated as 23.3% among species of the same genus, 25% among genera of the same family, and 1.7% at intraspecific level with a few rare exceptions having a large difference, indicating a possibility of presence of cryptic species. Our data show that COI is a very appropriate marker for species identification of bryozoans, but does not provide enough phylogenetic information at higher taxonomic ranks. Greater effort involving larger taxon sampling for the barcode analyses is needed for bryozoan taxonomy.

• Animal Systematics, Evolution and Diversity
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• 제36권3호
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• pp.240-250
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• 2020

Member of the genus Tobrilus Andrássy, 1959, which belongs to Tobrilidae Filipjev, 1918, are known as free-living nematodes in freshwater habitat. This genus was previously unknown from Korea. Five Tobrilus species are here reported for the first time from Korea: Tobrilus aberrans (Schneider, 1925), Tobrilus diversipapillatus (Daday, 1905), Tobrilus gracilis (Bastian, 1865), Tobrilus longus (Leidy, 1851), and Tobrilus wesenbergi (Micoletzky, 1925). Specimens were collected from sediments of the Nakdong River in Korea. Morphological characters and measurements of the specimens generally agree with the original descriptions of Tobrilus species, except for some differences that can be attributed to intraspecific variation among populations(e.g., nerve ring position [% pharynx] and reproductive length). Each species can be distinguished from other members of the genus by specific characters (e.g., cephalic setae length and position, buccal cavity and pocket shape, vulva position, degree of development of reproductive system, and tail length and shape). Here, five species in the genus Tobrilus are fully redescribed and illustrated using optical microscopy images. DNA barcode sequence information (the D2-D3 region of 28S rDNA) is also provided for molecular species identification.

• Animal Systematics, Evolution and Diversity
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• 제37권4호
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• pp.273-279
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• 2021

Branchinotogluma segonzaci (Miura and Desbruyères, 1995) occurs in hydrothermal vent fields of the southwestern Pacific Ocean. We morphologically compared B. segonzaci from the North Fiji Basin with the original description from the Lau Basin and a subsequent study of specimens from the Manus Basin. The main characteristics of all B. segonzaci populations were similar having 21 segments, 10 pairs of elytra, cylindrical-shaped anterior lobes, and ventral papillae on segment 12 and ventral lamellae on segments 13-17 in males. However, the specimens from the North Fiji Basin had rounded to sub-renifrom elytra rather than oval in the original description. Additionally, we newly obtained 11 cytochrome oxidase subunit I (COI) DNA barcodes from the North Fiji Basin and Tonga Arc populations and compared them with known COI DNA barcodes of Branchinotogluma species. Thirteen sequences of B. segonzaci showed 0.0-1.07% intraspecific variation and formed two clades in the COI neighbor-joining tree, whereas the interspecific variation among Branchinotogluma species was 8.19-22.4%. The results of this study contribute to biogeographic studies of B. segonzaci and the evolution of polynoid scale worms in chemosynthesis-based ecosystems.