• Title/Summary/Keyword: Intra-Variability

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Update on Distribution and Genetic Variability of Plum pox virus Strains in Bulgaria

  • Kamenova, Ivanka;Borisova, Anelija
    • The Plant Pathology Journal
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    • v.35 no.3
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    • pp.243-256
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    • 2019
  • Field surveys for Plum pox virus (PPV) infection were conducted in stone fruit orchards all over Bulgaria. In total, 1168 out of 3020 leaf samples from cultivated Prunus spp. and wildly growing P. cerasifera trees reacted positive for PPV in DASI-ELISA with the universal monoclonal antibody (MAb) 5B. Further ELISA analyses showed that 987 and 127 isolates belonged to PPV-M and PPV-D serotypes, respectively. The plum and P. cerasifera showed 82.0% and 50.5% levels of infection, respectively followed by the peach (40.0%) and the apricot (32.0%). Five hundred fifty one PPV isolates were further typed by IC-RT-PCR with PPV-Rec, -M and -D-specific primers, targeting (Cter)NIb-(Nter) CP genome region, as 125 isolates were sequenced. The results revealed the presence of PPV-Rec, PPV-M and PPV-D and mixed infections of these strains. PPV-Rec was the most prevalent strain (49.0%), followed by PPV-M (40.1%), while PPV-D was the less spread strain (8.2%). PPV-Rec was the most common strain in plums, including the eight "old-aged" trees from the region of the first Sharka discovery. PPV-M was the most prevalent strain in peach and apricot. Phylogenetic analyses on (Cter)NIb-(Nter)CP of the isolates were performed. PPV-Rec isolates formed a homogeneous group, while PPV-M isolates split into PPV-Ma and PPV-Mb subgroups. Five separated clades were formed by the analyzed PPV-D isolates. Nucleotide sequences of the partial CP coding region of the analyzed isolates revealed a slightly higher intra-strain genetic variability in PPV-Rec and PPV-M isolates, while that of PPV-D strain isolates was higher from the reported for these strains.

Spatio-temporal variability of future wind energy over the Korean Peninsular using Climate Change Scenarios (기후변화 시나리오를 활용한 한반도 미래 풍력에너지의 시공간적 변동성 전망)

  • Kim, Yumi;Lim, Yoon-Jin;Lee, Hyun-Kyoung;Choi, Byoung-Choel
    • Journal of the Korean Geographical Society
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    • v.49 no.6
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    • pp.833-848
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    • 2014
  • The assessment of the current and future climate change-induced potential wind energy is an important issue in the planning and operations of wind farm. Here, the authors analyze spatiotemporal characteristics and variabilities of wind energy over Korean Peninsula in the near future (2006-2040) using Representative Concentration Pathway(RCP) scenarios data. In this study, National Institute of Meteorological Research (NIMR) regional climate model HadGEM3-RA based RCP 2.6 and 8.5 scenarios are analyzed. The comparison between ERA-interim and HadGEM3-RA during the period of 1981-2005 indicates that the historical simulation of HadGEM3-RA slightly overestimates (underestimates) the wind energy over the land (ocean). It also shows that interannual and intraseasonal variability of hindcast data is generally larger than those of reanalysis data. The investigation of RCP scenarios based future wind energy presents that future wind energy density will increase over the land and decrease over the ocean. The increase in the wind energy and its variability is particularly significant over the mountains and coastal areas, such as Jeju island in future global warming. More detailed analysis presents that the changes in synoptic conditions over East Asia in future decades can influence on the predicted wind energy abovementioned. It is also suggested that the uncertainty of the predicted future wind energy may increase because of the increase of interannual and intra-annual variability. In conclusion, our results can be used as a background data for devising a plan to develop and operate wind farm over the Korean Peninsula.

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UPWELLING FILAMENTS AND THEIR ROLE IN CROSSFRONTAL WATER EXCHANGE

  • Kostianoy, A.G.;Soloviev, D.M.
    • Proceedings of the KSRS Conference
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    • v.2
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    • pp.954-957
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    • 2006
  • Satellite data (thermal and color imagery) show that offshore flowing filaments off the west coasts of North America, North and South Africa can influence significantly the cross-frontal mixing in the coastal upwelling zones. To evaluate this role, we investigated structure, dynamics and behavior of surface filaments in the Canary and Benguela upwelling regions on the base of daily satellite IR and VIS imagery (AVHRR NOAA, MODIS-Aqua). It was found that seasonal variability of the filaments location depends on intra-annual shift of general upwelling intensity along the coast. The main statistical characteristics of filaments - length, width, temperature anomaly and estimates of velocity were obtained. Estimates of cross-frontal water exchange due to filamentation based on the statistical data show that these coherent structures play a major role in the water and particle exchange between coastal zone and the open ocean in both upwelling regions.

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Automated Drug Infusion System Based on Fuzzy PID Control during Acute Hypotension

  • Kashihara, Koji
    • 제어로봇시스템학회:학술대회논문집
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    • 2005.06a
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    • pp.186-189
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    • 2005
  • In a clinical setting, developing a reliable method for the automated drug infusion system would improve a drug therapy under the unexpected and acute changes of hemodynamics. The conventional proportional-integral-derivative (PID) controller might not be able to achieve maximum performance because of the unexpected change of the intra- and inter-patient variability. The fuzzy PID control and the conventional PID control were tested under the unexpected response of mean arterial blood pressure (MAP) to a vasopressor agent during acute hypotension. Compared with the conventional PID control, the fuzzy PID control performed the robust MAP regulation regardless of the unexpected MAP response (average absolute value of the error between target value and actual MAP: 0.98 vs. 2.93 mmHg in twice response of the expected MAP and 2.59 vs. 9.75 mmHg in three-times response of the expected MAP). The result was due to the adaptive change of the proportional gain in PID parameters.

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An Improvement of Korean Speech Recognition Using a Compensation of the Speaking Rate by the Ratio of a Vowel length (모음길이 비율에 따른 발화속도 보상을 이용한 한국어 음성인식 성능향상)

  • 박준배;김태준;최성용;이정현
    • Proceedings of the IEEK Conference
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    • 2003.11b
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    • pp.195-198
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    • 2003
  • The accuracy of automatic speech recognition system depends on the presence of background noise and speaker variability such as sex, intonation of speech, and speaking rate. Specially, the speaking rate of both inter-speaker and intra-speaker is a serious cause of mis-recognition. In this paper, we propose the compensation method of the speaking rate by the ratio of each vowel's length in a phrase. First the number of feature vectors in a phrase is estimated by the information of speaking rate. Second, the estimated number of feature vectors is assigned to each syllable of the phrase according to the ratio of its vowel length. Finally, the process of feature vector extraction is operated by the number that assigned to each syllable in the phrase. As a result the accuracy of automatic speech recognition was improved using the proposed compensation method of the speaking rate.

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Visual Presentation of Connected Speech Test (CST)

  • Jeong, Ok-Ran;Lee, Sang-Heun;Cho, Tae-Hwan
    • Speech Sciences
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    • v.3
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    • pp.26-37
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    • 1998
  • The Connected Speech Test (CST) was developed to test hearing aid performance using realistic stimuli (Connected speech) presented in a background of noise with a visible speaker. The CST has not been investigated as a measure of speech reading ability using the visual portion of the CST only. Thirty subjects were administered the 48 test lists of the CST using visual presentation mode only. Statistically significant differences were found between the 48 test lists and between the 12 passages of the CST (48 passages divided into 12 groups of 4 lists which were averaged.). No significant differences were found between male and female subjects; however, in all but one case, females scored better than males. No significant differences were found between students in communication disorders and students in other departments. Intra- and inter-subject variability across test lists and passages was high. Suggestions for further research include changing the scoring of the CST to be more contextually based and changing the speaker for the CST.

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Variation of Leaf Form of Leaf Variabilities of Natural Population of Quercus spp. (참나무 천연집단(天然集團)의 엽형변이(葉型變異))

  • Kim, Chi Moon;Kwon, Ki Won;Moon, Heung Kyu
    • Journal of Korean Society of Forest Science
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    • v.71 no.1
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    • pp.82-89
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    • 1985
  • Morphological characteristics of leaves of 10 natural populations of Quercus mongolica and 9 natural populations of Q. serrata were investigated to study inter-population and intra-population variability as well as interspecies variability. Five leaf form indices, blade length/max. blade width, blade length/petiole length, blade length/vein number, upper 1/3 blade width/max.blade width, upper 1/3 blade width/lower 1/3 blade width, as well as petiole length and vein number were included in the present study of Leaf variabilities of the Quercus population. All of the investigated leaf variabilities except for the case of blade length/petiole length, in Q. serrata indicated highly significant differences among the populations and among the individual trees within population. Both of Q. mongolica and Q. serrata represented different leaf forms between southern populations and northern populations in the indices of blade length/max. blade width, blade length/petiole length and blade length/vein number, and so the leaf forms of northern populations were more similar to the unique characters of Q. mongolica and those of southern populations were more similar to the unique characters of Q. serrata. The variability among individual trees within population was more notable in blade length/petiole length, and petiole length than in other leaf form indices, and also it was more obvious for Mt. Kyeryrong population located in middle part of the country than for other population.

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Variability of Transrectal Shear Wave Elastography in a Phantom Model (팬텀연구에서 경직장 전단파탄성초음파의 가변성)

  • Jihyun Lee;Seong Kuk Yoon;Jin Han Cho;Hee Jin Kwon;Dong Won Kim;Jun Woo Lee
    • Journal of the Korean Society of Radiology
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    • v.84 no.5
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    • pp.1110-1122
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    • 2023
  • Purpose This study aimed to assess the variability of transrectal shear wave elastography (SWE) using a designed phantom. Materials and Methods In a phantom, the SWE values were examined by two radiologists using agarose and emulsion silicone of different sizes (1, 2, and 3 cm) and shapes (round, cubic) at three depths (1, 2, and 3 cm), two region of interest (ROI) and locations (central, peripheral) using two ultrasound machines (A, B from different vendors). Variability was evaluated using the coefficient of variation (CV). Results The CVs decreased with increasing phantom size. Significant changes in SWE values included; agarose phantom at 3 cm depth (p < 0.001; machine A), 1 cm depth (p = 0.01; machine B), emulsion silicone at 2 cm depth (p = 0.047, p = 0.020; both machines). The CVs increased with increasing depth. Significant changes in SWE values included; 1 cm agarose (p = 0.037, p = 0.021; both machines) and 2 cm agarose phantom (p = 0.047; machine A). Significant differences in SWE values were observed between the shapes for emulsion silicone phantom (p = 0.032; machines A) and between ROI locations on machine B (p ≤ 0.001). The SWE values differed significantly between the two machines (p < 0.05). The intra-/inter-operator agreements were excellent (intraclass correlation coefficient > 0.9). Conclusion The phantom size, depth, and different machines affected the variability of transrectal SWE.

A Method for Observation of Benign, Premalignant and Malignant Changes in Clinical Skin Tissue Samples via FT -IR Microspectroscopy

  • Skrebova, Natalja;Aizawa, Katsuo;Ozaki, Yukihiro;Arase, Seiji
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.457-459
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    • 2002
  • Sunlight causes various types of adverse skin changes on the sun-exposed areas of the skin, in which the most hazardous one is the induction of malignant skin tumours. FT -IR spectra were obtained from specimens excised from normal skin, BCCs, SCCs, MMs, nevi, lesions of solar keratosis and Bowen's disease. Tissue samples from freshly frozen specimens were cut into 2 sections in strictly sequential order to be stained with H & E for histopathological analysis, and then to be air-dried on CaF$_2$ slide glasses for further spectral data acquisition from defined area of interest. Intra- and inter-sample variations were estimated within grouped lesion categories according to each skin component. Mean spectra for each type of tissue pathology in the 800-1800 $cm^{-1}$ / region was interpreted using the classical group frequency approach that showed the most visible differences in spectra of benign, premalignant and malignant changes directly related to protein conformation and nucleic acid bases. The relative intensity of the nucleic acid peak was increased with progression to malignancy. In addition, PCA was able to evaluate and maximise the differences in the spectra by reducing the number of variables characterizing each patient and pathology category. This type of approach to non-destructively estimate the complexity of IR-spectra of inhomogeneous samples such as skin demonstrates the advantage of FT -IR microspectroscopy to be able to observe diseased states (benign, premalignant, malignant) and distinguish them from normal against a huge background of inter- and intra-subject variability.

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Intraobserver and interobserver reproducibility in linear measurements on axial images obtained by cone-beam computed tomography

  • Silva, Nathalia Cristine da;Barriviera, Mauricio;Junqueira, Jose Luiz Cintra;Panzarella, Francine Kuhl;Raitz, Ricardo
    • Imaging Science in Dentistry
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    • v.47 no.1
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    • pp.11-15
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    • 2017
  • Purpose: This study was performed to investigate the intra- and inter-observer variability in linear measurements with axial images obtained by PreXion (PreXion Inc., San Mateo, USA) and i-CAT(Imaging Sciences International, Xoran Technologies Inc., Hatfield, USA) CBCT scanners, with different voxel sizes. Materials and Methods: A cylindrical object made from nylon with radiopaque markers (phantom) was scanned by i-CAT and PreXion 3D devices. For each axial image, measurements were taken twice in the horizontal(distance A-B) and vertical (distance C-D) directions, randomly, with a one-week interval between measurements, by four oral radiologists with five years or more experience in the use of these measuring tools. Results: All of the obtained linear measurements had lower values than those of the phantom. The statistical analysis showed high intra- and inter-observer reliability (p=0.297). Compared to the real measurements, the measurements obtained using the i-CAT device and PreXion tomography, on average, revealed absolute errors ranging from 0.22 to 0.59 mm and from 0.23 to 0.63 mm, respectively. Conclusion: It can be concluded that both scanners are accurate, although the linear measurements are underestimations, with no significant differences between the evaluators.