• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.389-396
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• 2016

This study examined the improving effect of DFA IV obtained from bacterial fermentation on the gut health. The effects of the dietary DFA IV on the intestinal mass, short chain fatty acids production and pH were evaluated in vivo. Sprague-Dawley rats were fed the 0% (control) or 1% DFA IV supplemented diets for 3 weeks. Supplementation of DFA IV resulted in a significant increase in cecal tissue and wall weights. Together with the lowering of the cecal and colonic pH, the amount of acetate and butyrate increased by 1.6 and 3.2 fold of the control group in the cecum, respectively, in the rats fed DFA IV diets. The DFA IV diet also significantly increased the cecal lactate 1.5 fold compared to the control diet, indicating that dietary DFA IV stimulated the growth of lactic acid bacteria and bifidobacteria in the intestine. Based on the above results, it is concluded that the dietary DFA IV may be used as a putative prebiotic supplement.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.1
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• pp.64-70
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• 2017

Objective: In the present study, role of increasing levels of Ecklonia cava (seaweed) supplementation in diets was investigated on growth performance, coefficient of total tract apparent digestibility (CTTAD) of nutrients, serum immunoglobulins, cecal microflora and intestinal morphology of weanling pigs. Methods: A total of 200 weaned pigs (Landrace${\times}$Yorkshire${\times}$Duroc; initial body weight $7.08{\pm}0.15kg$) were randomly allotted to 4 treatments on the basis of body weight. There were 5 replicate pens in each treatment including 10 pigs of each. Treatments were divided by dietary Ecklonia cava supplementation levels (0%, 0.05%, 0.1%, or 0.15%) in growing-finishing diets. There were 2 diet formulation phases throughout the experiment. The pigs were offered the diets ad libitum for the entire period of experiment in meal form. Results: The pigs fed with increasing dietary concentrations of Ecklonia cava had linear increase (p<0.05) in the overall average daily gain, however, there were no significant differences in gain to feed ratio, CTTAD of dry matter and crude protein at both phase I and phase II. Digestibility of gross energy was linearly improved (p<0.05) in phase II. At day 28, pigs fed Ecklonia cava had greater (linear, p<0.05) Lactobacillus spp., fewer Escherichia coli (E. coli) spp. (linear, p<0.05) and a tendency to have fewer cecal Clostridium spp. (p = 0.077). The total anaerobic bacteria were not affected with supplementation of Ecklonia cava in diets. Polynomial contrasts analysis revealed that villus height of the ileum exhibited a linear increase (p<0.05) in response with the increase in the level of dietary Ecklonia cava. However, villus height of duodenum and jejunum, crypt depth, villus height to crypt depth ratio of different segments of the intestine were not affected. Conclusion: The results suggest that Ecklonia cava had beneficial effects on the growth performance, cecal microflora, and intestinal morphology of weanling pigs.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.1
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• pp.48-54
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• 2007

Bacillus polyfermenticus SCD (B. polyfermenticus SCD) has been appropriately used for the therapy of long-term intestinal disorders, because live strains in the form of active endospores can successfully reach the target intestine in humans. B. polyfermenticus SCD produces the most antibiotic-related materials. In the present study, B. polyfermenticus SCD was fermented with soybeans (BPFS) and its probiotic properties were investigated. B. polyfermenticus SCD and BPFS showed a broad spectrum of antimicrobial activity against pathogenic Gram-positive (Streptococcus parauberis, Streptococcus iniae, Lactococcus garviae) and Gram-negative (Flexibacter tractuosus, Vibrio harveyi, Vibrio vulnificus, Vibrio ordalii) bacteria and moulds (Aspergillus niger, Aspergillus oryzae). Sebastes schlegeli were used to examine survival rate and cleanup action by BPFS. Bacterial infection resulted in a mortality of up to 99% in the commercial fodder fed groups. BPFS both enhanced the growth rate of fry by improving their appetite and had cleaned by decreased eutrophication. Therefore, it seems appropriate that BPFS should be developed as an antibiotic replacement, favorable fodder additive, and antifungal material in fish farming systems.

• Korean Journal of Poultry Science
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• v.42 no.4
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• pp.291-297
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• 2015

We investigated the effects of dietary pearlzyme (mudflat-bacteria origin protease) on growth performance and development of digestive organs in broilers. Two hundred forty, 4 day-old female Ross broiler chicks were divided into 2 groups (control vs. pearlzyme) which were randomly housed in 8 pens with 15 chicks/pen. They were fed one of two diets containing pearlzyme at 0 or 0.1% for 4 weeks. Dietary pearlzyme resulted in significant increase in body weight during the first week of the experiment (p<0.05). With age, weight gain and feed efficiency continued to decrease reaching significant level during the last week. Mortality was 3.3% in control but not in pearlzyme during the entire period of the experiment. Dietary pearlzyme resulted in increased weight (p<0.05) in the ceca and rectum, and increased length in the ceca (p<0.05). However, there were tendencies to increase the weight of the gizzard (p<0.071) but to decrease the length of the small intestine (p<0.068). The results of the current study show that dietary pearlzyme affects weight gain and the development of digestive organs.

• Journal of Life Science
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• v.20 no.1
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• pp.77-81
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• 2010

We separated the bacteria showing protease activity from Reticulitermes speratus which is known as the only termite species in Korea. Then, we collected the best activated strain and studied the optimal culture condition for producing the enzyme. According to the results of observing morphological and physiological characteristics, and the type of 16S rRNA of the strain, it was identified as Serratia marcescens and named S. marcescens strain TM-3215. This strain showed the best activity under conditions of 0.8% (w/v) starch, 0.4% (w/v) peptone, 0.08% (w/v) $MgSO_4{\cdot}7H_2O$, $30^{\circ}C$ and pH 8.0. After being cultivated under optimal conditions for 9 hr, the strain produced 19.8 U/ml of enzyme, an amount 1.8 times greater than the control.

• Journal of Veterinary Clinics
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• v.26 no.3
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• pp.226-230
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• 2009

We evaluated the efficacy of $\beta$-hemolytic Streptococcus(S.) iniae vaccine on cultured olive flounder. Three hundred flounders(weight $50{\pm}5$ g) were obtained from two farm at Wando and Taean in the southern and western coast of Korea at May and June 2007, respectively. Twenty of flounders moved in 0.5 tons aquaria in land-marine tank system of National Veterinary Research and Quarantine Service. Seawater was transported from the sea of Inchon in western Korea, and water temperature maintained to $22^{\circ}C$ and $25^{\circ}C$ during the vaccination and challenge test, respectively. We used the formalin-inactivated $\beta$-hemolytic S. iniae vaccine produced by domestic manufacturers. The vaccine was intraperitoneally administered to fish. The vaccinated and control group were challenged with intraperitoneal injection by virulent S. iniae SI-36 isolates with $5.0{\times}10^8$ CFU/fish at 3 weeks after vaccination. We evaluated the vaccine efficacy by calculating numbers of dead fish, and observing of clinical signs, exterior and gross lesions, and examining bacteria isolation and identification. Thirty-four(25.2%) of 135 control and vaccinated group fish were dead with serious anemia, abdominal extension, and hernia of intestine during 3 weeks post vaccination. We isolated Neoheterobothrium hirame from the buccal cavity and Edwardsiella tarda from kidney of dead and diseased fish. When infected fish with these agents were challenged with S. iniae SI-36 isolates, the cumulative mortality of control and vaccinated group were 86.7, and 46.7%, respectively. However, significant differences(p<0.05) were observed on cumulative mortality between control(20.0%) and vaccinated group(95.0%) at second trials with 40 healthy, and relative percent survival(RPS) was 78.0%. We confirmed that the efficacy of $\beta$-hemolytic S. iniae vaccine on olive flounder were impacted by health condition such as bacterial and parasitic diseases.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.11
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• pp.827-833
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• 2011

In this study, fecal nonpoint pollutant sources tracking were conducted on Ansan stream. Multiple Antibiotic Resistance Analysis (MARA) method used in this study is based on the premise that fecal bacteria derived from intestine of human or animal has each different resistance for antibiotics. First of all, a database for known sources should be established to use the method and then, an unknown sample was applied on the database to find unknown sources by statistical analysis. The Ansan stream was considered with divided condition into three parts: upper (livestock farming area), mid (old section of the city), and downstream (new section of the city) to search an environmental influence of the stream basin. As results of the statistical analysis, it could be estimated that the upper stream area was influenced by animals due to the nature of influence for the livestock farms located in this area because livestock were classified as percentages of 45.8% in 3-way method divided into livestock, pet and human. In case of midstream and downstream, the human influence was remarkable as percentage of 60% and 80%, respectively. From these results, it could be judged that the MARA method is useful in source tracking the non-point pollutant sources because the MARA results correspond to which predictable non-point pollutant sources by a field study. Also, it is expected that a more effective source tracking will be possible as establishing database of each area.

• The Korean Journal of Food And Nutrition
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• v.28 no.2
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• pp.171-177
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• 2015

Sodium butyrate is a short-chain fatty acid derivative found in foods, such as Parmesan cheese and butter and is produced by anaerobic bacteria fermentation of dietary fibers in the large intestine. There have been reports that butyrate prevented obesity, protected insulin sensitivity, and ameliorated dyslipidemia in dietary obese mice. This study investigated the effects of sodium butyrate on fasting blood glucose level and serum lipid profile in streptozotocin(STZ)-induced diabetic mice. Male C57BL/6 mice were fed AIN-93G for four weeks prior to intraperitoneal injections with STZ (100 mg/kg body weight). Diabetic mice had supplements of 5% sodium butyrate for four weeks. The 5% sodium butyrate diet significantly improved fasting blood glucose level and lipid profile in STZ-induced diabetic mice. Inflammation has been recognized to decrease beta cell insulin secretion and increase insulin resistance. Circulating cytokines can directly affect beta cell function, leading to secretory dysfunction and increased apoptosis. Thus, anti-inflammatory therapies represented a potential approach for the therapy of diabetes and its complications. In this animal study, the 5% sodium butyrate supplementation also inhibited inflammatory cytokine production in STZ-induced diabetic mice. These results suggested that sodium butyrate can be a potential candidate for the prevention of diabetes and its complications.

• Korean Journal of Poultry Science
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• v.47 no.4
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• pp.275-283
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• 2020

This experiment was conducted to investigate the effect of the addition of enzymes and microorganisms to broiler feed on productivity, carcass characteristics, intestinal microflora, and feces odor. A total of one-hundred eighty 180 chicks (Ross 308) were randomly assigned to 5 treatments with 3 replications each having 12 birds per pen. The experimental group was divided into 0.1% EZ group (0.1% metallo-protease added to the feed), 0.2% EZ group (0.2% metallo-protease added to the feed), M group (2.0% Bacillus veleznesis CE 100 added to the feed), and MW group (2.0% Bacillus veleznesis CE 100 added to the feed and drinking water). In the results, final body weight, body weight gain, the feed conversion ratio, protein efficiency, and energy efficiency were not significantly different among all treatments in across all periods. Carcass weight, proventriculus, gizzard, heart, small intestine, cecum, and rectum weight were not significantly different among all of the treatments. However the liver weight was significantly higher in the 0.1% EZ group than in the control, M and MW groups (P< 0.05). E. coli was significantly lower in MW than in the control and M (P<0.05), and it was significantly higher in the M than 0.2% EZ and MW (P<0.05). H2S emissions in feces was not significantly different among all treatments, but NH3 emissions was were significantly higher in 0.1% EZ than in MW (P<0.05). In conclusion, the addition of 0.1% of metallo-protease was effective in the development of the liver of broilers.

• Animal Bioscience
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• v.35 no.10
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• pp.1461-1478
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• 2022

The maintenance of poultry gut health is complex depending on the intricate balance among diet, the commensal microbiota, and the mucosa, including the gut epithelium and the superimposing mucus layer. Changes in microflora composition and abundance can confer beneficial or detrimental effects on fowl. Antibiotics have devastating impacts on altering the landscape of gut microbiota, which further leads to antibiotic resistance or spread the pathogenic populations. By eliciting the landscape of gut microbiota, strategies should be made to break down the regulatory signals of pathogenic bacteria. The optional strategy of conferring dietary fibers (DFs) can be used to counterbalance the gut microbiota. DFs are the non-starch carbohydrates indigestible by host endogenous enzymes but can be fermented by symbiotic microbiota to produce short-chain fatty acids (SCFAs). This is one of the primary modes through which the gut microbiota interacts and communicate with the host. The majority of SCFAs are produced in the large intestine (particularly in the caecum), where they are taken up by the enterocytes or transported through portal vein circulation into the bloodstream. Recent shreds of evidence have elucidated that SCFAs affect the gut and modulate the tissues and organs either by activating G-protein-coupled receptors or affecting epigenetic modifications in the genome through inducing histone acetylase activities and inhibiting histone deacetylases. Thus, in this way, SCFAs vastly influence poultry health by promoting energy regulation, mucosal integrity, immune homeostasis, and immune maturation. In this review article, we will focus on DFs, which directly interact with gut microbes and lead to the production of SCFAs. Further, we will discuss the current molecular mechanisms of how SCFAs are generated, transported, and modulated the pro-and anti-inflammatory immune responses against pathogens and host physiology and gut health.