• 한국산림과학회지
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• 제96권3호
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• pp.290-294
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• 2007

참나무속(Q. serrata, Q. dentata, Q. mongolica var. crispula, Q. aliena)의 인공교배에 의 한 종간잡종의 묘목을 조취(鳥取)대학농학부 구내의 묘포장에 식재하였다. 또한 조취(鳥取)대학 산산(蒜山)연습림에서 자연교잡종(Q. fabri와 $Q.{\times}mccormickii$) 가계의 묘목을 선발하여 조취(鳥取)대학 산산(蒜山)연습림에 식재하였다. 인공종간잡종 $F_1$은 4년생, 자연교잡종 $F_1$은 3년생 때에 개화하였다. 2001년도에 이 중에서 개화한 개체로부터 화분과 종자를 채취하여 화분의 크기와 임성, 종자의 활력을 조사하였다. 인공종간잡종 화분의 발아율은 1개체를 제외하고 84% 이상이었고, 화분관의 신장도 양호하였다. 인공종간잡종의 건전한 종자의 발아율은 90% 이상으로 양친과 비슷한 높은 발아율을 보였다. 자연잡종 가계의 종자도 건전한 종자의 발아율은 각 가계의 평균이 64% 이상이었고, Q. fabri, $Q.{\times}mccormickii$의 자식종자도 높은 번식 능력을 유지하는 것으로 나타났다.

• 미생물학회지
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• 제29권1호
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• pp.8-15
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• 1991

Interspecific hybrids between the ASpergillus spp., A. awamori, A. usamii and A. oryzae, were obtained by nuclear transfer technique. Nuclei isolated from an auxotrophic mutant strain were transferred into the protoplasts of a recipient strain of different species. The frequency of interspecific hybrid formation by nuclear transfer was $2*10^{-5}$ $-7*10^{-4}$ In contrast, no interspecific hybrid was isolated by protoplast fusion. Among the hybrids tested, 10 strains showed increased activity of some or all components of cellulases, xylanases and amylase up to more than two times. Isozyme pattern of the hybrids were analyzed by polyacrylamide gel electrophoresis and isoelectric focusing followed by activity staining, which showed that some of the hybrids have isozyme patterns unidentical to either of the two parents. By measuring the DNA contents and the sizes ofthe conidia, the karyotypes of the hybrids were estimated to be aneuploid near to haploid, diploid or triploid. It was concluded that the unclear transfer technique is much more efficient in the formation of interspecific hybrids than protoplast fusion and is very useful for the improvement of Aspergillus strains.

• Journal of Ginseng Research
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• 제21권2호
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• pp.85-90
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• 1997

This study was carried out to clarify the cause of incompatibility in interspecific hybrid plant between Panax ginseng and p. quinquefolium. The floral structure of F,(p.g. x p.q.) hybrid was normal because the redundant anther was 0.2 mm longer than pistil in Fl hybrid and the size and structure of redundant carpel in F, hybrid were similar to P. ginseng and p. quiquefolium Pollens of $F_1$ hybrid did not germinate on stigma of P-quinquefolium but germinated well on stigma of P. ginseng. Pollen tube was able to penetrate styles completely and seed harvest rate was 16.8% in field. However on stigma of $F_1$ hybrid, Pollen did not germinate when P. ginseng was used as male Parent. In addition, the growth of pollen tube was halted on style and seed was not set when P qlfinquefoEi2a was used as male Parent. These suggest that the inhibitor of pollen germination present on stigma caused $F_1$ hybrid sterility. It took 5 hours for pollen grains to germinate, 12 hours to arrive at in trance of ovule, 16 hours to penetrate micropyles in Panax ginseng.

• Journal of Forest and Environmental Science
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• 제37권1호
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• pp.35-43
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• 2021

The interspecific hybrid of Salix caprea and Salix gracilistyla has never been identified or studied in Korea. Accordingly, this study investigated the flower morphological characteristics of the interspecific hybrid between S. caprea and S. gracilistyla and compared the interspecific hybrid with S. caprea and S. gracilistyla, respectively. The female flowers were investigated for 12 characteristics and the male flowers were investigated for nine. For the female flowers, those of the hybrids were larger than those of S. caprea and S. gracilistyla in terms of catkin length (CL), bract length (BL), and bract width (BW). The hybrids are intermediates between S. caprea and S. gracilistyla in terms of ovary length, width, and stipitate length as well as gland length (GL). For the male flowers, those of the hybrids were bigger than those of S. caprea and S. gracilistyla in terms of CL, BL, and BW. The hybrids are intermediates between S. caprea and S. gracilistyla in terms of catkin width and stamen length (SL). A principal component analysis (PCA) of the female data showed that the first principal component (PC) explained 57.5% of the total variation. The first PC highly correlated the ovary stipitate and pistil style lengths. The analysis was divided into three groups of S. caprea, S. gracilistyla, and the hybrid by the first PC. The results of a PCA of the male data showed that the first PC explained 35.7% of the total variation. The first PC highly correlated with the adelphous SL and was divided into three groups of S. caprea, S. gracilistyla, and the hybrid. The results of the discriminant analysis showed that S. caprea, S. gracilistyla, and the hybrid were distinguishable by flower morphological characteristics. Therefore, the hybrid was distinctly separated from S. caprea and S. gracilistyla by flower characteristics.

• 미생물학회지
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• 제24권2호
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• pp.91-97
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• 1986

Intra and interspecfic fusants were produced by the protoplast fusion of auxotrophic mutants from Trichoderma koningii ATCC 26113 and Trichoderma reesei QM 9414. It was found that 0.6M $MgSO_4\;and\;0.6M\;NH_4Cl$ was the best osmotic stabilizer for the preparation of protoplasts from the mycelium of T. koningii and T. reesei respectively. However, $MgSO_4$ was the most suitable one for the regeneration of the protoplasts from both species. The intraspecific protoplast fusion frequencies between the auxotrophic mutants from T. reesei were $1.8{\times}10^{-2}\;to\;5.1{\times}10^{-1}$. Interspecific protoplast fusion frequencies between the auxotrophic mutants from T. koningii and T. reesei were $3.6{\times}10^{-3}$\;to\;8.4{\times}10^{-2}. Interspecific complementing fusants, however, were not alwats produced. Fusants obtained from interspecific potoplast fusion were spontaneously segregated into various strains including parental types, non-parental auxotrophic hybrids, and prototrophic hybrids on complete plate. Interspecific hybrids revealed to have partially enhanced celluloytic activities.

• 한국초지조사료학회지
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• 제8권3호
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• pp.123-127
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• 1988

Even though grass species are one of major importance in livestock industry, the response of intergeneric hybridization of Lolium and Festuca and interspecific hybridization of Lolium m.X Lolium p. to Korean climatic condition has not been adequately documented. This reasearch was conducted to examine the growth characteristics and dry matter yield of Festulolium, cv. Felopa and C-Stamm N 66 and hybrid ryegrass, cv. Maja and Tetilelite. 1. The early growth of Italian ryegrass was better than that of hybrid ryegrass and Festulolium. The plant length and helght of Italian ryegrass were longerltaller than those of hybrid ryegrass and Festulolium, but the number of tillers of Italian ryegrass was less than that of hybrid ryegrass and Festulolium. 2. Festulolium and hybrid ryegrass were more tolerant than Italian xyegrass to winter killing, 70-80% of tillers was survived. 3. After wintering, early spring growth of hybrid ryegrass and Festulolium were better than that of Italian ryegrass. Dry matter yield of hybrid ryegrass and Festulolium were more than that of Italian ryegrass. 4. Spikes of hybrid ryegrass and Festulolium (C-Stamm N66) were more than those of Italian ryegrass. Seed yield per $m^2$ of hybrid ryegrass and Italian ryegrass were almost same, but that of Festulolium was lower.

• 한국초지조사료학회지
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• 제8권2호
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• pp.123-127
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• 1988

Even though grass species are one of major importance in livestock industry, the response of intergeneric hybridization of Lolium and Festuca and interspecific hybridization of Lolium m.X Lolium p. to Korean climatic condition has not been adequately documented. This reasearch was conducted to examine the growth characteristics and dry matter yield of Festulolium, cv. Felopa and C-Stamm N 66 and hybrid ryegrass, cv. Maja and Tetilelite. 1. The early growth of Italian ryegrass was better than that of hybrid ryegrass and Festulolium. The plant length and height of Italian ryegrass were longer/taller than those of hybrid ryegrass and Festulolium, but the number of tillers of Italian ryegrass was less than that of hybrid ryegrass and Festulolium. 2. Festuloliu and hybrid ryegrass were more tolerant than Italian ryegrass to winter killing, 70-80% of tillers was survived. 3. After wintering, early spring growth of hybrid ryegrass and Festulolium were better than that of Italian ryegrass. Dry matter yield of hybrid ryegrass and Festulolium were more than that of Italian ryegrass. 4. Spikes of hybrid ryegrass and Festulolium (C-Stamm N66) were more than those of Italian ryegrass. Seed yield per $m^2$ of hybrid ryegrass and Italian ryegrass were almost same, but that of Festuloliu was lower.

• Journal of Life Science
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• 제10권1호
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• pp.48-50
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• 2000

The cytogenetic pattern of autosome and sex chromiosome after Giemas staining were examined in the hybrids between two sibling species, Drosophila melanogaster and D. simulans. The analysis of karyotype in the hybrid female between D. melanogaster females and D. simulans males could be easily distinguished the characteriation of eight chromosomes from bothe species, especially with regard to X chromosomes. The lagging duplication of Y chromosome was investigated in the interspecific hybrid males from the cross between female of Drosophila melanogaster(OR) and males of D. simulasn (K18). On the other hand, the X chromatids of D. simulans were loosely separated in the early stage of anaphase.

• 한국연초학회지
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• 제17권2호
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• pp.109-113
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• 1995

Nicotiana repanda has resistance to many important tobacco diseases but no tobacco cultivars are currently available that carry risistance genes derived from this species. Numerous attempts to hybridize N, repanda with commercial tobacco cultivars have been largely unsuccessful because of cross incompatibility or the uncovering of lethal genes. In vitro pollination of placenta attached ovules was useful in by passing prezygotic barriers for interspecific hybrid combination between N.tabacum cv. NC82 and N. repanda. Six days after in vitro pollination of N. tabacum cv. NC82×N. repanda, enlarged ovules on plancenta were removed and transferred into ovule culture medium of kitsch and kitsch (1969). Within 15 days of ovule culture, germination occurred. Most of the hybrid seedlings obtained had poor root system and finally died, while few of them had good root system and grew well.

• 한국자원식물학회지
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• 제28권1호
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• pp.119-125
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• 2015

본 연구는 유채-MS를 이용하여 배추와 순무 등을 화분친으로 활용 잎의 모용이 없는 유채 잎에 배추와 순무의 엽색과 엽형을 도입하여 소비자가 선호하는 새로운 형질을 갖춘 엽채소의 작출 가능성을 검토하고자 실시하였다. 교배효율에 영향을 미치는 인공수분 후 주두 위에서의 화분발아나 화분관 신장은 유채-MS와 배추간 종간 교배 조합에서 유채-MS와 순무간 종간교잡보다 빠르게 진행되었으며, 유채-MS와 배추간 종간교잡에서의 결협율이 90.6%로 유채-MS와 순무의 교배 결협율 67.3% 보다 23.3% 정도 높게 나타났으며, 협당결실립수는 각각 15.5립, 11.6립으로 나타나 유채-MS와 배추간의 종간교잡 효율이 높았다. 유채-MS와 배추의 종간교잡 $F_1$ 잡종의 표현형질의 특성은 주로 모계인 유채와 비슷하였으며 엽색은 밝은 녹색으로 양친의 중간형을 나타냈고 모용은 적게 분포되어 있었으며 엽수는 10.2매, 엽장은 27.5 cm, 엽폭은 14.0 cm로 잎의 길이와 넓이가 유채에 비하여 커졌다. 유채-MS와 순무의 종간교잡 $F_1$ 잡종의 엽수는 9.6매, 엽장은 21.6 cm, 엽폭은 10.6 cm로 잎의 크기, 형태 및 결각 등은 양친의 중간형을 나타냈으며 엽색은 모본인 유채와 비슷하거나 좀 더 짙은 녹색이었고, 모용이 잎의 앞과 뒷면에 존재하였다.