• Asian-Australasian Journal of Animal Sciences
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• 제24권1호
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• pp.1-8
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• 2011

Large quantities of high-quality recipient oocytes with uniform cytoplasm are needed for research in the promising field of somatic cell nuclear transfer (SCNT) and embryonic stem cell research. In canines, however, it is difficult to obtain large quantities of oocytes because each donor produces a limited number of mature oocytes in vivo. Although in vitro maturation (IVM) is considered an alternative approach to oocyte production, this technique is still too rudimentary to be used for the production of highquality, uniform oocytes in large quantities. One technique for overcoming this difficulty is to use oocytes obtained from different species. This technique is known as interspecies SCNT (iSCNT). This review provides an overview of recent advances in canine - porcine interspecies SCNT.

• Asian-Australasian Journal of Animal Sciences
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• 제5권3호
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• pp.511-517
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• 1992

Ethanol was utilized by mixed rumen microbes, but addition of pentachlorophenol (25 mg/l), a methanogen inhibitor, suppressed the utilization of ethanol. Carbon monoxide (50% of the gas phase), a hydrogenase inhibitor, more strongly suppressed the utilization of ethanol, propanol, and butanol. These results suggest that the major ethanol utilizers are $H_2$ producers. Ethanol utilization was depressed at low pH (below 6.0). Since methanogens were shown to be relatively resistant to low pH, it appears that ethanol utilizers are particularly sensitive to low pH. Ruminococcus albus and R. flavefaciens in mono-culture produced ethanol from carbohydrate (glucose and cellobiose), even when a high level (170 mM) of ethanol was present. Ethanol was not utilized even in the absence of carbohydrate, but the co-culture of these bacteria with methanogens resulted in the utilization of ethanol, i.e., when $H_2$ was rapidly converted to $CH_4$, R. albus and R. flavefaciens utilized ethanol. These results suggest that ethanol is utilized when the electrons liberated by the oxidation of ethanol are rapidly removed, and ready electron disposal in ethanol-utilizing, $H_2$-producing bacteria is accomplished by the interspecies transfer of $H_2$.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.801-806
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• 2008

A classical technique to study somitic cell fate is to employ the cross-transplantation of quail somites into a chick host. The densely stained nucleoli of the quail cells makes it possible to assess the fate of the donor quail cells in the chick host. Classical somite transplantation techniques have been hampered by the necessity of a small opening in the chick eggshell, difficulty in hatching the offspring and interspecies post-hatch graft rejection. With the advent of transgenic chicken technology, it is now possible to use embryos from transgenic chickens expressing reporter genes in somite cross-transplantation techniques to remove any possibility of interspecies graft rejection. This report describes using a surrogate eggshell system in conjunction with transgenic chick:chick somitic cell cross-transplantation to generate viable chimeric embryos and offspring. Greater than 40% of manipulated embryos survive past 10 days of incubation, and ~80% of embryos successfully cultured past 10 days of incubation hatched to produce viable offspring.

• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1665-1672
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• 2008

Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studies of iSCNT using murine cells and bovine oocytes have been reported, the development of murine-bovine iSCNT embryos beyond the 8-cell stage has not been successful. In this paper, we examined the developmental potential of embryos reconstructed with a murine embryonic fibroblast as the nuclear donor and a bovine oocyte as the cytoplasm recipient. The reconstructed embryos were cultured in CZB (murine medium) or CR1aa (bovine medium). In addition, for the development of a murine-bovine iSCNT blastocyst, the antioxidant ${\beta}$-mercaptoethanol (${\beta}ME$) was supplemented to CR1aa medium. Furthermore, to verify the mouse genome activation in murine-bovine iSCNT embryos, RT-PCR analysis of murine Xist was performed. The development of the murine-bovine iSCNT embryos cultured in CR1aa was significantly higher than that in CZB (p<0.05). With respect to the effect of BME on the development of the murine-bovine iSCNT blastocyst, addition of BME produced a significant increase in blastocyst development (p<0.05). Karyotype analysis confirmed that the reconstructed embryos were derived from murine cells (40XX). The Xist gene was gradually increased from the 8-cell stage to the blastocyst stage. This is the first report of blastocyst development of iSCNT embryos derived from murine somatic cells and bovine oocytes. These results demonstrate that bovine cytoplasm can support the development of later stages of a preimplantation embryo from murine-bovine iSCNT.

• Animal Bioscience
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• 제37권2_spc호
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• pp.323-336
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• 2024

Molecular hydrogen (H₂) and formate (HCOO^-) are metabolic end products of many primary fermenters in the rumen ecosystem. Both play a vital role in fermentation where they are electron sinks for individual microbes in an anaerobic environment that lacks external electron acceptors. If H₂ and/or formate accumulate within the rumen, the ability of primary fermenters to regenerate electron carriers may be inhibited and microbial metabolism and growth disrupted. Consequently, H₂- and/or formate-consuming microbes such as methanogens and possibly homoacetogens play a key role in maintaining the metabolic efficiency of primary fermenters. There is increasing interest in identifying approaches to manipulate the rumen ecosystem for the benefit of the host and the environment. As H₂ and formate are important mediators of interspecies interactions, an understanding of their production and utilization could be a significant starting point for the development of successful interventions aimed at redirecting electron flow and reducing methane emissions. We conclude by discussing in brief ruminant methane mitigation approaches as a model to help understand the fate of H₂ and formate in the rumen ecosystem.

• 한국환경생태학회지
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• 제37권5호
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• pp.392-402
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• 2023

본 연구는 천관산도립공원의 식물사회 특성을 파악하고 지속가능한 식생관리와 도립공원 연구에 필요한 기초자료를 구축하기 위해 수행되었다. 천관산도립공원을 대상으로 126개의 방형구를 설치하여 출현한 목본수종을 조사하였고 희소종을 제외한 종을 중심으로 종간결합분석을 실시하였다. 그 결과를 Gephi 0.10 프로그램을 이용하여 소시오그램으로 작성하였으며, 인접한 노드 간 그룹을 나누어주는 모듈화 분석과 네트워크 중심성 및 구조분석을 실시하였다. 분석 결과, 조사된 방형구 내 출현빈도는 청미래덩굴이 가장 높게 나타났고, 졸참나무, 사스레피나무, 때죽나무, 조릿대 등의 순으로 높게 나타났다. 희소종을 제외한 69종에 대해 종간결합분석을 실시하였고, 양성결합을 토대로 식물사회네트워크를 시각화하였다. 식물사회네트워크는 69개의 노드와 396개의 연결선으로 구성되어 있으며, 한 수종이 평균 약 17.9종과 종간결합을 맺으며 2.3단계 만에 서로 간 연결되었다. 69종은 모듈화 분석을 통해 3개의 그룹으로 나뉘었으며, 1그룹은 주로 상록활엽수 및 난온대 지역에서 출현하는 수종으로 이루어졌으며, 2그룹은 낙엽활엽수 위주로 이루어졌다. 3그룹은 주로 햇빛이 잘 들고 건조한 양지에서 잘 자라는 수종으로 나뉘었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권6호
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• pp.602-610
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• 2008

Purpose Acrylamide is present in significant quantities in a wide range of commonly consumed human foods. Carcinogenic risk of acrylamide through the consumption of food is a great public concern and in controversy, but it is not properly addressed due to the lack of evidence in humans. While a plenty of data is available on the carcinogenicity in animal models, the studies in humans are limited. Thus, the present study attempted to examine the carcinogenic potentials of acrylamide on the human epithelial cell, which is the target cell origin of the most cancers. Material and method & Result 1. Acrylamide was not cytotoxic up to $100{\mu}M$ as measured by MTT and LDH assays, indicating a relatively low toxicity of this substance in human epithelial cells. 2. The parameters of neoplastic cellular transformation such as cell saturation density, soft-agar colony formation and cell aggregation were analyzed to examine the carcinogenic potential of acrylamide. 3. The neoplastic transformation was further increased with the co-treatment of TPA 4. Antioxidants blocked the generation of Reactive Oxygen Species(ROS) and the GSH depleting agent dramatically increased the ROS production. 5. mRNA levels of fibronectin following acrylamide exposure was increased in a dose-dependent manner, indicating a possible biomarker of acrylamide-induced cellular transformation. Conclusion The present study will provide a valuable basis to compare the interspecies differences in response to carcinogenic potentials of acrylamide. The data on the interspecies differences are essential element in human risk assessment. Thus, our results obtained from the human epithelial cells will contribute to improving the risk assessment of human neoplasm including oral cancer.

• 유기물자원화
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• 제31권1호
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• pp.15-26
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• 2023

혐기소화에 의한 메탄생산은 유기물이 가수분해, 산생성 단계를 거쳐 아세트산생성균과 메탄생성균 간의 영양공생 (syntrophy)에 의해 일어난다. 본 연구에서는 종간 영양공생 기작인 종간직접전자전달 (DIET, Direct Interspecies Electron Transfer) 과정을 촉진시키기 위하여 전도체인 마그네타이트 (Fe₃O₄) 첨가가 음폐수의 메탄생산에 미치는 영향을 파악하고자 하였다. 이를 위해, 본 연구에서는 회분식 혐기반응기를 이용하여 마그네타이트 투입량에 따른 음폐수의 메탄퍼텐셜 (B_u)과 최대메탄생산속도 [R_m(t₀)]를 분석하였다. 마그네타이트 무처리구의 메탄퍼텐셜은 0.496 Nm³/kg-VS_added이었으며, 21.06일에 38.24 mL/day의 최대메탄생산속도를 보였다. 마그네타이트 5, 10, 15, 20, 25, 30, 40, 70, 100mM 처리구의 메탄퍼텐셜은 각각 0.502, 0.498, 0.512, 0.510, 0.518, 0.523, 0.524, 0.540, 0.549 Nm³/kg-VS_added이었으며, 마그네타이트 투입량 증가에 따라 유의성 있는 메탄퍼텐셜의 증가 경향을 보였다. 최대메탄생산속도는 무처리구와 비교하여 마그네타이트 처리구에서 증가하였으며 15mM 처리구에서 36.95%까지 증가하였다. 또한, 마그네타이트 투입농도가 증가함에 따라 최대메탄생산속도에 도달하는 기간(t₀)은 무처리 21.06일에서 마그네타이트 100mM 처리 14.67일로 크게 단축되었다. 따라서, 마그네타이트 투입에 따른 음폐수의 메탄퍼텐셜과 최대메탄생산속도가 크게 향상되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권5호
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• pp.748-763
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• 2003

Anaerobic rumen microorganisms mainly bacteria, protozoa and fungi degrade ligno-cellulosic feeds consumed by the ruminants. The ruminants in developing countries are predominantly maintained on low grade roughage and grazing on degraded range land resulting in their poor nutrient utilization and productivity. Hence, manipulation of rumen fermentation was tried during last two decades to optimize ruminal fermentation for improving nutrient utilization and productivity of the animals. Modification of rumen microbial composition and their activity was attempted by using chemical additives those selectively effect rumen microbes, introduction of naturally occurring or genetically modified foreign microbes into the rumen and genetically manipulation of existing microbes in the rumen ecosystem. Accordingly, rumen protozoa were eliminated by defaunation for reducing ruminal methane production and increasing protein outflow in the intestine, resulting in improve growth and feed conversion efficiency of the animals. Further, Interspecies trans-inoculation of rumen microbes was also successfully used for annulment of dietary toxic factor. Additionally, probiotics of bacterial and yeast origin have been used in animal feeding to stabilize rumen fermentation, reduced incidence of diarrhoea and thus improving growth and feed conversion efficiency of young stalk. It is envisaged that genetic manipulation of rumen microorganisms has enormous research potential in developing countries. In view of feed resource availability more emphasis has to be given for manipulating rumen fermentation to increase cellulolytic activity for efficient utilization of low grade roughage.

• 한국환경생태학회지
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• 제34권3호
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• pp.259-270
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• 2020

식물사회네트워크란 식물사회를 이해하기 위한 기존의 식물사회학적 방법과 사회과학에서 최근에 주목받고 있는 사회연결망 분석 방법을 접목하여 식물사회연결망을 시각화하고 분석하는 것을 말한다. 구축 및 분석 과정은 조사구 설정 및 출현 수종 조사, 종간결합분석, 소시오그램 작성, 네트워크 구조 및 중심성 분석 순으로 진행된다. 식물사회네트워크를 구축하기 위해 본 연구는 해안식생과 내륙식생을 동시에 볼 수 있는 부산광역시를 중심으로 다양한 상관우점식생이 포함되도록 708개 조사구를 설치하여 출현 수종을 조사하였다. 조사 결과, 출현한 수종은 모두 195종이었으며, 상록수 42종, 낙엽수 151종, 반상록수 2종으로 나탔으며, 전체 출현수종을 중심으로 종간결합분석을 실시하였다. 종간결합 분석 결과, 친화종수는 사스레피나무(47종), 마삭줄(46종), 감태나무(44종), 팥배나무(44종), 광나무(41종) 순으로 나타났으며, 이를 바탕으로 gephi 0.9.2. program을 활용하여 소시오그램을 작성하였다. 작성된 소시오그램은 해안에서 주로 출현하는 그룹과 그렇지 않은 그룹으로 나뉘어져 있어 부산광역시 산림식생의 지리적 분포특성을 반영하고 있었다. 네트워크 구조를 분석한 결과, 1,709개의 연결선(link)이 나타났고, 한 수종과 종간결합을 갖는 종수의 평균은 약 17.5개였다. 밀도는 0.09, 지름은 5, 평균 경로거리는 2.268로 분석되었는데, 사회과학분야의 네트워크 특성과 정밀한 비교 분석을 위해 앞으로 다양한 식물사회네트워크 구축이 진행되어야 할 것으로 판단되었다. 부산광역시 식물사회네트워크에서는 사스레피나무, 감태나무, 광나무, 마삭줄 등이 중심성이 높은 것으로 나타났다.