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Metabolic engineering for biofortification of lipophilic antioxidants in plants (식물의 지용성 항산화 물질 생산 증대를 위한 대사공학 연구현황)

  • Kim, Eun-Ha;Lee, Kyeong-Ryeol;Kim, Jong-Bum;Roh, Kyung Hee;Kang, Han Chul;Kim, Hyun Uk
    • Journal of Plant Biotechnology
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    • v.41 no.4
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    • pp.169-179
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    • 2014
  • Intracellular antioxidants include low molecular weight scavengers of oxidizing species, and enzymes which degrade superoxide and hydroperoxides. Such antioxidants systems prevent oxidative damage to cellular component by scavenging free radicals and activated oxygen species. Hydrophobic scavengers are found in cell membrane where they interrupt chain reactions of lipid peroxidation. The three major lipophilic antioxidant classes for human health are carotenoids, vitamin E and coenzyme Q10. The biofortification of staple crops with these lipid soluble antioxidants is an attractive strategy to increase the nutritional quality of human food. Here, we have summarized the biosynthetic pathways of three lipid soluble antioxidants in plants and current status of genetic engineered plants for elevated levels of each lipophilic antioxidant.

Clinical Analysis of Intraoperative Rupture of Cerebral Aneurysms (수술 중 뇌동맥류 파열에 대한 임상 분석)

  • Baek, Won-Cheol;Koh, Hyeon-Song;Kim, Youn
    • Journal of Korean Neurosurgical Society
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    • v.30 no.sup1
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    • pp.73-78
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    • 2001
  • Objective : Intraoperative rupture of an intracranial aneurysm can interrupt a microsurgical procedure and jeopardize the patient's chance to favorable outcome. The purpose of this study was to analyse and evaluate intraoperative aneurysmal rupture and render ideal prevention and management to intraoperative rupture. Patients and Methods : The authors retrospectively analysed the results of 609 patients who underwent cerebral aneurysm surgery from January 1991 to December 2000. Results : 1) Intraoperative aneurysmal rupture occurred in 73 of 609 consecutive aneurysm surgery, so the incidence was about 12.0% and it was relatively lower than other reports. 2) Aneurysms arising from anterior communicating artery appeared more prone to intraoperative rupture. 3) The size of aneurysm and timing of operation didn't influence intraoperative aneurysmal rupture and temporary clipping didn't reduce the incidence of intraoperative aneurysmal rupture. 4) Intraoperative aneurysmal rupture occured during three specific periods : (1) dissection stage in 61%, (2) clip application stage in 29 %, (3) predissection stage in 10%. 5) In the patients with intraoperative aneurysmal rupture, surgical outcome was relatively good and there was no significant difference in outcome compared with unruptured group. Conclusion : Our suggestion for prevention methods of intraoperative aneurysmal rupture are as follows : 1) minimal brain retraction, 2) sharp and careful aneurysmal neck dissection, 3) gentle clipping with proper clip selection etc. Management methods after intraoperative aneurysmal rupture are as follows : 1) strong aspiration of bleeding point, 2) rapid application of temporary and/or tentative clip, 3) following rapid dissection of neck and proper clip application, 4) use of encircling clip etc.

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Airway Management with Reinforced Laryngeal in Mask Airway in a Child with Hypoplastic Left Heart Syndrome (좌심실형성부전증후군 환아의 치과치료를 위한 전신마취 시 강화 후두마스크를 이용한 기도 유지)

  • Kim, Kyung Jin;Shin, Teo-Jeon;Hyun, Hong-Keun;Kim, Young-Jae;Kim, Jung-Wook;Jang, Ki-Taeg;Lee, Sang-Hoon;Kim, Chong-Chul;Seo, Kwang-Suk
    • Journal of The Korean Dental Society of Anesthesiology
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    • v.13 no.4
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    • pp.221-224
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    • 2013
  • Despite the laryngeal mask airway (LMA) has been widely used in anesthesia, its use is rare in the field of dentistry. Placing LMA in the oral cavity may interrupt dental treatment. However, there are some circumstances in which LMA is more advantageous than tracheal intubation for managing the airway. Especially, the reinforced LMA has a flexible tube shaft, rendering it more accessible for dental treatment. We report a case of dental treatment of a 3-year-old patient with hypoplastic left heart syndrome combined with pulmonary hypertension using reinforced LMA for airway management under general anesthesia. We also discuss the considerations of utilizing the reinforced LMA for dental treatment.

Development of Standing and Moving Human Body Sensing Module Using a Chopper of Shutter Method (셔터방식의 쵸퍼를 이용한 정지 및 이동인체 감지 모듈 개발)

  • Cha, Hyeong-Woo;Lee, Won-Ho
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.2
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    • pp.109-116
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    • 2016
  • Sensing module of standing and moving human body using shutter method was developed. The module consists of Fresnel lens, pyroelectric infrared (PIR) sensor, interface circuit of the PIR, micro control unit(MCU), and alarm light emitting diode(LED). The principle for standing human body is chopping the thermal of human body using camera shutter. The human sensing signal in MCU by algorithm of interrupt function is detected. By unifying an apparatus and print circuit board(PCB), the developed module can be replaced as commercial moving human body detector. Experiment results show that sensing distance is about 7.0m and sensing angles is about $110^{\circ}$ at room temperature. In these condition, sending ratio was 100% and the power dissipation of the module was 100mW.

Assessment on Water Movement in Paddy-Upland Rotation Soil Scheduled for Ginseng Cultivation (답전윤환 인삼재배 예정지 토양의 물 이동특성 평가)

  • Hur, Seung-Oh;Lee, Yun-Jeong;Yeon, Byung-Ryul;Jeon, Sang-Ho;Ha, Sang-Geon;Kim, Jeong-Gyu
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.3
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    • pp.204-209
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    • 2009
  • This study was conducted to assess water movement in paddy-upland rotation soil scheduled for ginseng cultivation through the measurement of infiltration and permeability of soil water. Soil sample was divided with four soil layers. The first soil layer (to 30cm from top soil) was loamy sand, the second and the third soil layers (30$\sim$70 ㎝) were sand, and the fourth (< 120 ㎝) was sandy loam. The soil below 130 ㎝ of fourth soil layer was submerged under water. The shear strength, which represents the resisting power of soil against external force, was 3.1 kPa in the first soil layer. This corresponded to 1/8 of those of another soil layer and this value could result in soil erosion by small amount of rainfall. The rates of infiltration and permeability depending on soil layers were 39.86 cm $hr^{-1}$ in top soil, 2.34 cm $hr^{-1}$ in 30$\sim$70 ㎝ soil layer, 5.23 cm $hr^{-1}$ and 0.18 cm $hr^{-1}$ in 70$\sim$120 ㎝ soil layer, with drain tile, and without drain tile, respectively. We consider that ground water pooled in paddy soil and artificial formation of soil layer could interrupt water canal within soil and affect negatively on water movement. Therefore, we suggest that to drain at 5 m intervals be preferable when it makes soil dressing or soil accumulation to cultivate ginseng in paddy-upland rotation soil to reduce failure risk of ginseng cultivation.

Influences of Hinge Region of a Systhetic Antimicrobial Peptide, Cecropin A(1-13)-Melittin(1-13) Hybrid on Antibiotic Activity

  • 신송엽;강주현;이동건;장소윤;서무열;김길룡;함경수
    • Bulletin of the Korean Chemical Society
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    • v.20 no.9
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    • pp.1078-1084
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    • 1999
  • A synthetic cecropin A(1-13)-melittin(1-13) [CA-ME] hybrid peptide was known to be an antimicrobial peptide having strong antibacterial, antifungal and antitumor activity with minimal cytotoxic effect against human erythrocyte. Analogues were synthesized to investigate the influences of the flexible hinge region of CA-ME on the antibiotic activity. Antibiotic activity of the peptides was measured by the growth inhibition against bac-terial, fungal and tumor cells and vesicle-aggregating or disrupting activity. The deletion of Gln-Gly-Ile (P1) or Gly-Gln-Gly-Ile-Gly (P3) from CA-ME brought about a significant decrease on the antibiotic activities. In contrast, Gly-Ile-Gly deletion (P2) from CA-ME or Pro insertion (P5) instead of Gly-Gln-Gly-Ile-Gly of CA-ME retained antibiotic activity. This result indicated that the flexible hinge or β-bend structure provided by Gly-Gln-Gly-Ile-Gly, Gln-Gly, or Pro in the central region of the peptides is requisite for its effective antibiotic activity and may facilitate easily the hydrophobic C-terminal region of the peptide to penetrate the lipid bilayers of the target cell membrane. In contrast, P4 and P6 with Gly-Gln-Gly-Pro-Gly or Gly-Gln-Pro in the central region of the peptide caused a drastic reduction on the antibiotic activities. This result suggested that the con-secutive β-bend structure provided by Gly-Gln-Gly-Pro-Gly or Gly-Gln-Pro in the central hinge region of the peptide seems to interrupt the ion channel/pore formation on the target cell membranes.

State Transition Model-based Design of Wireless Gateway Types to Connect between a Sub-network of Things and Mobile Internet and their Performance Evaluations (사물 서브 망과 모바일 인터넷을 연계하는 무선 게이트웨이 타입들의 상태천이모델 기반 설계와 성능 평가)

  • Seong, Cheol-Je;Kim, Changhwa
    • Journal of the Korea Society for Simulation
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    • v.25 no.3
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    • pp.1-14
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    • 2016
  • This paper proposes four general wireless gateway types, which are distinguished by their own processing ways to connect between a wireless sub-network of things and the mobile internet that links mobile network to internet step by step. In this paper, we also design general processing procedures of these four types using the state transition model. Gateways of each types were developed on the basis of the resulted state transition models and their performances were evaluated through several tests, analyzed, and compared each other. As the results of our evaluation, compared with the other types, the type, which combines both of a low-power Sleep-interrupt way and polling ways for receiving data or responses in all the waiting states of a gateway, shows the best performance in all of data transmission real-timeliness, data loss and energy consumption.

Development of Catenary Stagger and Height Measurement System using Laser (레이저를 이용한 전차선 편위 및 높이 측정 시스템 개발)

  • Song, Sung-Gun;Lee, Teak-Hee;Song, Jae-Yeol;Park, Seong-Mo
    • Journal of the Korean Society for Railway
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    • v.11 no.6
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    • pp.569-574
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    • 2008
  • Catenary and Pantograph are used to transmit electrical energy to electric railways. Catenary (Overhead Contact Lines) should be installed precisely and managed for stable train operations. But external factors such as weather, temperature, etc., or aging affect catenary geometry. Changed catenary stagger and height cause high voltage spark or instant electric contact loss. Big spark derived from contact loss can damage the pantograph carbon strip and overhead contact lines that might interrupt the train operations. Therefore, to prevent a big scale spark or electric contact loss, catenary maintenance are required catenary geometry measurement systems with catenary maintenance capability. In this paper, we describe the development of catenary height and stagger measurement system. The catenary height and stagger measurement system uses Acuity company's AR4000 Laser Range Finder for distance measurement and AccuRange Line Scanner for degree measurement. This system detects suspicious overhead line sections with excessive stagger and height stagger variance.

Design and Evaluation of the Internet-Of-Small-Things Prototype Powered by a Solar Panel Integrated with a Supercapacitor

  • Park, Sangsoo
    • Journal of the Korea Society of Computer and Information
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    • v.26 no.11
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    • pp.11-19
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    • 2021
  • In this paper, we propose a prototype platform combined with the power management system using, as an auxiliary power storage device, a supercapacitor that can be fast charged and discharged with high power efficiency as well as semi-permanent charge and discharge cycle life. For the proposed platform, we designed a technique which is capable of detecting the state of power cutoff or resumption of power supplied from the solar panel in accordance with physical environment changes through an interrupt attached to the micro-controller was developed. To prevent data loss in a computing environment in which continuous power supply is not guaranteed, we implemented a low-level system software in the micro-controller to transfer program context and data in volatile memory to nonvolatile memory when power supply is cut off. Experimental results shows that supercapacitors effectively supply temporary power as auxiliary power storage devices. Various benchmarks also confirm that power state detection and transfer of program context and data from volatile memory to nonvolatile memory have low overhead.

Numerical Analysis of the Beach Stabilization Effect of an Asymmetric Ripple Mat (왜도 된 연흔모양 매트의 해빈 안정화 효과 수치해석)

  • Cho, Yong Jun
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.31 no.4
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    • pp.209-220
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    • 2019
  • Even though the scale of hard structures for beach stabilization should carefully be determined such that these structures do not interrupt the great yearly circulation process of beach sediment in which the self-healing ability of natural beach takes places, massive hard structures such as the submerged breakwater of wide-width are frequently deployed as the beach stabilization measures. On this rationale, asymmetric ripple mat by Irie et al. (1994) can be the alternatives for beach stabilization due to its small scale to replace the preferred submerged breaker of wide-width. The effectiveness of asymmetric ripple mat is determined by how effectively the vortices enforced at the contraction part of flow area over the mat traps the sediment moving toward the offshore by the run-down. In order to verify this hypothesis, we carry out the numerical simulations based on the Navier-Stokes equation and the physically-based morphology model. Numerical results show that the asymmetric ripple mat effectively capture the sediment by forced vortex enforced at the apex of asymmetric ripple mat, and bring these trapped sediments back to the beach, which has been regarded to be the driving mechanism of beach stabilization effect of asymmetric ripple mat.