• Food Science and Biotechnology
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• v.16 no.3
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• pp.337-342
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• 2007

Conventional methods for pathogen detection and identification are labor-intensive and take days to complete. Biosensors have shown great potential for the rapid detection of foodborne pathogens. Fiber-optic biosensors have been used to rapidly detect pathogens because they can be very sensitive and are simple to operate. However, many fiber-optic biosensors rely on manual sensor handling and the sandwich assay, which require more effort and are less sensitive. To increase the simplicity of operation and detection sensitivity, a binding inhibition assay method for detecting Listeria monocytogenes in food samples was developed using an automated, fiber-optic-based immunosensor: RAPTOR (Research International, Monroe, WA, USA). For the assay, fiber-optic biosensors were developed by the immobilization of Listeria antibodies on polystyrene fiber waveguides through a biotin-avidin reaction. Developed fiber-optic biosensors were incorporated into the RAPTOR to evaluate the detection of L. monocytogenes in frankfurter samples. The binding inhibition method combined with RAPTOR was sensitive enough to detect L. monocytogenes ($5.4{\times}10^7\;CFU/mL$) in a frankfurter sample.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.1
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• pp.79-82
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• 2003

To study the effect of mounting fully ripe and unripe silkworms at different maturation percentage, the ripe and unripe silkworms were mounted separately and observed the cocoon and reeling characters. The significant difference in the cocoon and reeling parameters was observed between all ripe and unripe batches except for summer season. The silkworms collecting together and mounting at a time can be recommended only in summer season and at certain level during rainy season.

• Korean Journal of Plant Taxonomy
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• v.43 no.2
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• pp.103-105
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• 2013

Capparis sikkimensis Kurz subsp. masaikai (H. L$\acute{e}$v.) Jacobs, is being reported as addition to the flora of Vietnam. Line drawing of the taxon has been provided in support of taxonomic treatment and to facilitate easy identification of the species.

• Research in Plant Disease
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• v.29 no.2
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• pp.108-117
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• 2023

With the development of advanced virus diagnostic technologies, numerous viruses, including novel viruses, have been identified explosively from various biological samples around the world over the last decade. For plant viruses, approximately 376 novel viruses have been reported in the last three years. Information on the occurrence and host ranges of plant viral diseases in a particular country or region is very important for diagnosis, quarantine, and control of the viral diseases. Recently, based on active research on the diagnosis and identification of plant viruses, a significant number of newly occurring viruses and new viral host plants have been reported in South Korea. This review paper provides integrated information on plant viruses and viral host plants newly reported in South Korea between 2020 and 2022 to help diagnose, control, and quarantine plant virus diseases in crop fields.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.1-6
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• 2001

This paper reports a few characteristics of seven insect mitochondrial genomes sequenced completely (Bombyx mori, Drosophila melanogaster, D. yakuba, Apis mellifera, Anopheles gambiae, A. quadrimaculatus, and Locusta migratoria). Comparative analysis of complete mt genome sequences from several species revealed a number of interesting features (base composition, gene content, A+T-rich region, and gene arrangement, etc) of insect mitochondrial genome. The properties revealed by our work shed new light on the organization and evolution of the insect mitochondrial genome and more importantly open up the way to clearly aimed experimental studies for understanding critical roles of the regulatory mechanisms (transcription and translation) in mitochondrial gene expression.

• Journal of Environmental Science International
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• v.33 no.6
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• pp.367-382
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• 2024

Alpine and subalpine plants are isolated and distributed in mountaintop areas at high altitudes and, are among the species most vulnerable to global warming. If the rate of warming continues at its current rate, it is highly likely that this species will be unable to find new refugia and will be the last to go extinct in their current habitats. Therefore, research on the distribution and diversity of alpine and subalpine plants is urgently needed and is important from the perspective of biodiversity conservation. Therefore, this study focused on the distribution of alpine and subalpine plants native to national parks. Alpine and subalpine plants distributed across the 12 national parks accounted for approximately half (47.78%) of all alpine plants in Korea. The average relative frequency of occurrence was 0.23, plant similarity between national parks was 37.19%, and beta diversity was 0.63. The number of species was positively correlated with the latitude, elevation, and area of the national park. Thus the geographical distributions of alpine and subalpine plants haves distinct spatial specificities and physical environmental differences.

• Korean Journal of Agricultural and Forest Meteorology
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• v.22 no.2
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• pp.79-91
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• 2020

Accurate assessment of greenhouse gas emissions is a cornerstone of every climate change response study, and reliable assessment of greenhouse gas emission data is being used as a practical basis for the entire climate change prediction and modeling studies. Essential, fundamental technologies for estimating greenhouse gas emissions include an on-site monitoring technology, an evaluation methodology of uncertainty in emission factors, and a verification technology for reductions. The closed chamber method is being commonly used to measure gas fluxes between soil-vegetation and atmosphere. This method has the advantages of being simple, easily available and economical. This study presented the technical bases of the closed chamber method for measuring methane fluxes from a rice paddy. The methane fluxes from rice paddies occupy the largest portion of a single source of greenhouse gas in the agricultural field. We reviewed the international and the domestic studies on automated chamber monitoring systems that have been developed from manually operated chambers. Based on this review, we discussed scientific concerns on chamber methods with a particular focus on quality control for improving measurement reliability of field data.

• Journal of Korea Trade
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• v.26 no.2
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• pp.65-83
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• 2022

Purpose - The purpose of this study is to evaluate the economic effects of FTAs using the concept of value-added exports. So far, the economic effects of FTAs have been dependent on decrease in import prices due to tariff cuts in importing countries, but the actual tariff reduction need to consider the value added of the exporting countries. Design/methodology - Value-added export refers to the added value created in the exporting country out of total exports. Among value-added exports, direct value-added export is interpreted as the Regional Value Contents (RVC), from which the economic effect of the FTA can be analyzed. A modified GTAP-VA model takes into account RVC in order to estimate accurate effects of FTAs. Findings - By the re-evaluation of the FTA based on the RVC, this paper makes it clear that the economic effects of the existing FTA methodology have the possibility of overestimation. In addition, as a new FTA with a strengthened Rules of Origin (ROO) is being initiated, a negative impact on international trade and GVC utilization may occur. Originality/value - This study introduces the concept of value-added export in analyzing the effects of FTAs. The new analysis methodology of this paper emphasizes the importance of value-added exports. Re-organization of GVCs would change regional trade agreements and empower ROO by weakening existing GVCs and transforming the value chain from global into regional scope.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.2
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• pp.97-104
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• 2019

This experiment was conducted to investigate the effect of whole crop rice (WCR) based TMR on growth performance and carcass characteristics of Hanwoo steers. WCR "Yeongwoo"was harvested at yellow ripen stage and ensiled for 60 days. The crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), in vitro dry matter digestibility (IVDMD) and total digestible nutrient (TDN) content was 8.4 %, 28.0 %, 53.8 %, 72.4 % and 66.8 %, respectively. For silage quality, pH was 4.37 and lactic and butyric acid content were 2.84 and 0.04 % in DM. Sixteen Hanwoo steers (8-mon-old) were allocated into either a control (commercial TMR) and WCR-TMR (WCR-based TMR) group. The TMR were fed according to the feeding stage phase: growing (Initiate~14 month), early fattening (15 month~21 month) and late fattening (22 month~30 month). The body weight of control group increased (P<0.05) until early fattening stage, but late growing stage of WCR-TMR group was higher than that of control (P<0.05). Average daily gain (ADG) was significantly greater (P<0.05) in WCR-TMR group (total 0.78 kg/head) compared to control (total 0.66 kg/head) except for late fattening stage. The marketing weight and carcass weight were higher in WCR-TMR group (726 vs 765 kg; 417.8 vs 450.4 kg). The back fat thickness (11.75 vs 13.00 mm), Longissimus dorsi area (88.00 vs $89.88cm^2$) and yield index (65.87 vs 64.30) were not different between the two groups (P>0.05) and also no difference in meat yield grade (A : B : C = 2 : 4 : 2). Marbling score (4.00 vs 4.13), meat color (4.75 vs 4.75), fat color (3.13 vs 2.88), texture (1.25 vs 1.50) and maturity (2.00 vs 2.00) were not significant difference between the two groups and meat quality grade ($1^{{+}{+}}:1^+:1:2:3=0:2:4:2:0$) was also not different. In conclusion, TMR feeding based on WCR silage showed superiority in carcass yield and ADG compared to control TMR. It is considered that the use of WCR for feed is a necessary option for the substitution of the imported forages and the government's policy for rice production adjustment.

• International Journal of Industrial Entomology and Biomaterials
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• v.27 no.2
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• pp.271-276
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• 2013

The Asian honeybee, Apis cerana, is a native honeybee species in Korea which is important in agriculture for pollination and honey production. For better understanding of the physiology of A. cerana, high-throughput Illumina transcriptome sequencing was performed to analyze the gene expression profiles of queen, worker, and larva. A total of 219,799,682 clean reads corresponding to 22.2 Gb of nucleotide sequences was obtained from the whole body total RNA samples. The Apis mellifera reference mRNA sequence database was used to measure the gene expression level with Bowtie2 and eXpress software, and the Illumina short reads were then mapped to 11,459 out of 11,736 A. mellifera reference genes. Total of 9,221 genes with FPKM value greater than 5 of each sample group were subjected to eggNOG with BLASTX for gene ontology analysis. The differential gene expression between queen and worker, and worker and larva were analyzed to screen the overexpressed genes in each sample group. In the queen and worker sample group, total of 1,766 genes were differentially expressed with 887 and 879 genes overexpressed over two folds in queen and worker, respectively. In the worker and larva sample group, total of 1,410 genes were differentially expressed with 1,009 and 401 genes overexpressed over two folds in worker and larva, respectively.