• Title/Summary/Keyword: International Agricultural Research Institute

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Prevalence of Phytophthora Blight of Pigeonpea in the Deccan Plateau of India

  • Sharma, M.;Pande, S.;Pathak, M.;Rao, J. Narayana;Kumar, P. Anil;Reddy, D. Madhusudan;Benagi, V.I.;Mahalinga, D.M.;Zhote, K.K.;Karanjkar, P.N.;Eksinghe, B.S.
    • The Plant Pathology Journal
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    • v.22 no.4
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    • pp.309-313
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    • 2006
  • Phytophthora blight(PB), caused by Phytophthora drechsleri f. sp. cajani is the third potentially important disease of pigeonpea in the Deccan Plateau(DP) of India after wilt and sterility mosaic. In the rainy-season of 2005, an outbreak of PB was seen throughout DP. To quantify the incidence and spread of the disease, a systematic survey was conducted in the major pigeonpea growing regions of DP during the crop season 2005. Attempts were made to determine the effect of cropping systems on the PB development and identify resistant cultivars, if any, grown by farmers and on research farms. Widespread incidence of PB was recorded on improved, and or local cultivars grown in different intercropping systems. Majority of improved cultivars grown at research farms were found susceptible to PB(>10% disease incidence). Pigeonpea intercropped with groundnut, black gram and coriander had less disease incidence(${\leq}10%$). Three wilt and SM resistant pigeonpea cultivars KPL 96053, ICPL 99044, and ICPL 93179 were found resistant(<10%) to PB as well. However, their resistance to PB needs confirmation under optimum disease development environments.

Analysis of the Genome Sequence of Strain GiC-126 of Gloeostereum incarnatum with Genetic Linkage Map

  • Jiang, Wan-Zhu;Yao, Fang-Jie;Fang, Ming;Lu, Li-Xin;Zhang, You-Min;Wang, Peng;Meng, Jing-Jing;Lu, Jia;Ma, Xiao-Xu;He, Qi;Shao, Kai-Sheng;Khan, Asif Ali;Wei, Yun-Hui
    • Mycobiology
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    • v.49 no.4
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    • pp.406-420
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    • 2021
  • Gloeostereum incarnatum has edible and medicinal value and was first cultivated and domesticated in China. We sequenced the G. incarnatum monokaryotic strain GiC-126 on an Illumina HiSeq X Ten system and obtained a 34.52-Mb genome assembly sequence that encoded 16,895 predicted genes. We combined the GiC-126 genome with the published genome of G. incarnatum strain CCMJ2665 to construct a genetic linkage map (GiC-126 genome) that had 10 linkage groups (LGs), and the 15 assembly sequences of CCMJ2665 were integrated into 8 LGs. We identified 1912 simple sequence repeat (SSR) loci and detected 700 genes containing 768 SSRs in the genome; 65 and 100 of them were annotated with gene ontology (GO) terms and KEGG pathways, respectively. Carbohydrate-active enzymes (CAZymes) were identified in 20 fungal genomes and annotated; among them, 144 CAZymes were annotated in the GiC-126 genome. The A mating-type locus (MAT-A) of G. incarnatum was located on scaffold885 at 38.9 cM of LG1 and was flanked by two homeodomain (HD1) genes, mip and beta-fg. Fourteen segregation distortion markers were detected in the genetic linkage map, all of which were skewed toward the parent GiC-126. They formed three segregation distortion regions (SDR1-SDR3), and 22 predictive genes were found in scaffold1920 where three segregation distortion markers were located in SDR1. In this study, we corrected and updated the genomic information of G. incarnatum. Our results will provide a theoretical basis for fine gene mapping, functional gene cloning, and genetic breeding the follow-up of G. incarnatum.

Comparison of Treatment Effect of Domestically Distributed Major Silage Inoculant

  • Young Sang Yu;Yan Fen Li;Xaysana Panyavong;Li Zhunang Wu;Jeong Ung Hwang;Li Li Wang;Hak Jin Kim;Won Jin Lee;Jong Geun Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.44 no.1
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    • pp.50-57
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    • 2024
  • Silage inoculants, crucial in modern silage production, comprise beneficial microorganisms, primarily lactic acid bacteria (LAB), strategically applied to forage material during ensiling. This study aimed to compare the effectiveness of various inoculants produced by different companies. Five treatments were evaluated, including a control group: T1 (Lactobacillus plantarum), T2 (Lactobacillus plantarum + Pediococcus pentosaceus), T3 (Lactobacillus plantarum + Pediococcus pentosaceus + Lactobacillus buchneri), T4 (Lactobacillus plantarum + Lactobacillus acidophilus + Lactobacillus bulgaricus), and T5 (Lactobacillus plantarum + Pediococcus pentosaceus + Enterococcus faecium). Italian ryegrass was harvested at the heading stage and treated with these silage inoculants. Samples were collected over a 60-day ensiling period. Co-inoculation with L. plantarum and P. pentosaceus (T2) resulted in significantly higher CP compared to the control group co-inoculation exhibited with resulted in Lactobacillus plantarum and Pediococcus pentosaceus in the T2 treatment exhibited higher CP content of 106.35 g/kg dry matter (DM). The T3 treatment, which included heterofermentative bacterial strains such as Lactobacillus buchneri, exhibited an increase in acetic acid concentration (11.15 g/kg DM). In the T4 treatment group, which utilized a mixed culture of Lactobacillus acidophilus and Lactobacillus bulgaricus, the NH3-N/TN content was observed to be the lowest (20.52 g/kg DM). The T5 containing Enterococcus faecium had the highest RFV (123) after 60 days. Expanding upon these findings, the study underscores not only the beneficial effects of particular inoculant treatments on silage quality but also underscores the potential of customized inoculation strategies in maximizing nutrient retention and overall silage preservation.

A LOW COST STRAW AND FORAGE CHOPPER

  • Pasikatan, M.C.;Salazar, G.C.;Quick, G.R.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1993.10a
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    • pp.686-695
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    • 1993
  • A flywheel-type, inclined axis chopper for small-area rice and livestock farmers, has been developed at IRRI Agricultural Engineering, The prototype is belt-driven by a 2.6kW engine and uses four angled blades rotating below a fixed counteredge. Manual feeding is facilitated by a convenient spout presenting the crop to the inclined blade housing and also suction created by the rotating blades . The distance between the rotating blades and the bottom of the housing determines the length of chops, set here for 25 cm. The unit would cost $200 without the engine. Tests with napier grass, corn stalks , and rice straw showed satisfactory performance within the acceptable clearance, speed and moisture content ranges of the material presented. Highest capacities were 1186, 1148 and 744kg/hr for napier grass, corn stalks and rice straw, respectively. Corn stalks required the highest power demand at 2.3kW engine would be adequate as power source. The chopper performance was comparable to higher cost commercial chippers in terms of capacity and specific energy.

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Influence of microbial additive on microbial populations, ensiling characteristics, and spoilage loss of delayed sealing silage of Napier grass

  • Cai, Yimin;Du, Zhumei;Yamasaki, Seishi;Nguluve, Damiao;Tinga, Benedito;Macome, Felicidade;Oya, Tetsuji
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.7
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    • pp.1103-1112
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    • 2020
  • Objective: To measure whether a microbial additive could effectively improve the fermentation quality of delayed-sealing (DS) silage, we studied the effects of inoculants of lactic acid bacteria (LAB) and cellulase enzyme on microbial populations, ensiling characteristics, and spoilage loss of DS silage of Napier grass in Africa. Methods: Quick-sealing (QS) and DS silages were prepared with and without LAB (Lactobacillus plantarum) inoculant, cellulase enzymes, and their combination. The QS material was directly chopped and packed into a bunker silo. The DS material was packed into the silo with a delay of 24 h from harvest. Results: In the QS silage, LAB was dominant in the microbial population and produced large amounts of lactic acid. When the silage was treated with LAB and cellulase, the fermentation quality was improved. In the DS silage, aerobic bacteria and yeasts were the dominant microbes and all the silages were of poor quality. The yeast and mold counts in the DS silage were high, and they increased rapidly during aerobic exposure. As a result, the DS silages spoiled faster than the QS silages upon aerobic exposure. Conclusion: DS results in poor silage fermentation and aerobic deterioration. The microbial additive improved QS silage fermentation but was not effective for DS silage.

A Biovoltine Silkworm Variety, Huayuan${\times}$Dongshen, That is Resistant to Fluoride Contamination

  • Xu, Anying;Lin, Changqi;Hou, Chengxiang;Zhang, Yuehua;Li, Muwang;Sun, Pingjiang
    • International Journal of Industrial Entomology and Biomaterials
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    • v.13 no.1
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    • pp.1-5
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    • 2006
  • The major dominant fluoride-endurance (Dfe) gene was introduced into the commercial varieties by crossing and pedigree selection to breed silkworm races that could normally develop in the area that polluted by fluoride. After backcrossed for two generations, the Dfe gene was made homozygous, and individuals with good economic characters were selected to generate next generation. After 8 generations of selection, their characters became stable, and the silkworm variety which is resistant to fluoride, Huayuan${\times}$Dongsheng, for spring rearing were bred.

Breeding of Near Isogenic Lines of Silkworm (Bombyx mori L.)

  • Li, Muwang;Xu, Anying;Hou, Chengxiang;Zhang, Yuehua;Huang, Junting;Guo, Xijie
    • International Journal of Industrial Entomology and Biomaterials
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    • v.6 no.2
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    • pp.207-210
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    • 2003
  • Four different backcrossing methods were designed and 23 near isogenic lines (NILs) of 22 linkage groups were obtained using Hb as recurrent parent, the mutant gene lines which held markers as donor parents. Eleven of them had been mated with the recurrent parent for 10 times, and the others for 7∼8 times. The NILs of other 6 linkage groups are under way and had been backcrossed to the recurrent for 3∼4 times. These NILs will act important roles in the construction of molecular linkage map and gene location and positional cloning.