• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.108-109
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• 2013

Mitochondria play key roles in the production of cell's energy. Their dominant function is the synthesis of adenosine 5'-triphosphate (ATP) from adenosine diphosphate (ADP) and phosphate (Pi) through the oxidative phosphorylation. Evaluation of drug-induced mitochondrial toxicity has become increasingly important since mitochondrial dysfunction has recently been implicated in numerous diseases including cancer and diabetes mellitus. Mitochondrial functions have been monitored via oxygen consumption, mitochondrial membrane potential, and more importantly via ATP synthesis since ATP synthesis is the most essential function of mitochondria. Various analytical methods have been employed to investigate ATP synthesis in mitochondria, including high performance liquid chromatography (HPLC), bioluminescence technique, and pH measurement. However, most of these methods are based on destructive analysis or indirect monitoring through the enzymatic reaction. Infrared absorption spectroscopy (IRAS) is one of the useful techniques for real-time, label-free, and direct monitoring of biological reactions [1,2]. However, the strong water absorption requires very short path length in the order of several micrometers. Transmission measurements with thin path length are not suitable for mitochondrial assays because solution handlings necessary for evaluating mitochondrial toxicity, such as rapid mixing of drugs and oxygen supply, are difficult in such a narrow space. On the other hand, IRAS in the multiple internal reflection (MIR) geometry provides an ideal optical configuration to combine solution handling and aqueous-phase measurement. We have recently reportedon a real-time monitoring of drug-induced necrotic and apoptotic cell death using MIR-IRAS [3,4]. Clear discrimination between viable and damaged cells has been demonstrated, showing a promise as a label-free and real-time detection for cell-based assays. In the present study, we have applied our MIR-IRAS system to mitochondria-based assays by monitoring ATP synthesis in isolated mitochondria from rat livers. Mitochondrial ATP synthesis and hydrolysis were in situ monitored with MIR-IRAS, while dissolved oxygen level and solution pH were simultaneously monitored with O2 and pH electrodes, respectively. It is demonstrated that ATP synthesis and hydrolysis can be monitored by the IR spectral changes in phosphate groups in adenine nucleotides and MIR-IRAS is useful for evaluating time-dependent drug effects of mitochondrial toxicants.

• Fire Science and Engineering
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• v.33 no.5
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• pp.149-154
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• 2019

The stability of firefighting pipes is crucial in the event of an earthquake. In Korea, specification-based designs are used in accordance with NFSC. However, engineering performance-based designs are used for buildings that have special requirements. For firefighting pipes, tree type pipe networks are usually utilized in buildings; however, they are characterized by several limitations. Hence, grid type and loop type networks are being utilized lately. Earthquake-resistant designs for firefighting pipes in Korea utilize NFPA 13 as the cookbook. Nevertheless, an engineering analysis is required to verify its reliability. The NFPA 13 standard used in Korea is a design method for engineers who lack earthquake engineering analysis knowledge of pipes and adapt ASCE and ASME guidelines. Earthquake resistant designs in Korea review braces only. Hence, various analyses under load conditions, such as the internal pressure of a pipe, force exerted by a continuous load, and an earthquake, are required to ensure reliability. An engineering earthquake-resistance analysis showed that tree type pipe networks are less stable than grid and loop type pipe networks. A comparison of earthquake-resistance analysis based on stress and strain revealed that strain analysis exhibited a conservative result value in the range of over-stress. Therefore, for the earthquake-resistance analysis of pipes, it is rational that engineers perform analysis to achieve the required standards through engineering analysis rather than uniform calculations, which should also be analyzed considering various analysis conditions.

• Land and Housing Review
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• v.4 no.1
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• pp.33-42
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• 2013

This study which are recognized that the lack of empirical research about the efficiency of the elements of environmentally friendly housing development planned presented housing design elements and policies to revitalize for the reduction of greenhouse gas emissions by analyzing the effectiveness of reduction of greenhouse gas output. In addition, it used various models of DEA which are accepted until now effective technique to evaluate the performance of the organization. In conclusion, there are effective 5 regionals which are Seoul, Incheon, Ulsan, South Chungcheong Province, South Gyeongsang Province. other regionals was analyzed to be inefficient. The conclusion from this study are as follows: First, in case of 11 regionals which are analyzed to be inefficient, they have to difference plan elements to make up. So each region should establish strategy to complement vulnerability. Second, not only internal architectural factors but institutional, and external environmental factors also affect the reduction of greenhouse gas emissions. And weighted scores also were moderately high. But levels of weighted scores still less than the ratio of Good quality housing. So it can be determined that evaluation of individual architecture still considered important. It need to pay more attention to the operating system and the external environmental factors.

• Membrane Journal
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• v.25 no.2
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• pp.132-143
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• 2015

Water is an essential resource for humans, but fresh water becomes scarce due to population growth and contamination of limited resources. Membrane technology has been widely used for water treatment, and forward osmosis is a process which does not need high hydraulic pressure for the operation. However, there are needs for (1) development of novel draw solutes causing low internal concentration polarization and reverse salt flux for high water flux, and (2) development of economic recovery method of the draw solutes in the diluted draw solution. Previous researches on the draw solute include $NaHCO_3$ which can be regenerated by about $60^{\circ}C$ heating, sucrose which can make potable water without additional process, and magnetic nanoparticles which can be regenerated by external magnetic field. Using the principles of forward osmosis process, sea water desalination, wastewater treatment, refinement of proteins, energy generation using pressure retarded osmosis process, preparation of diluted fertilizer, and growing algae for biofuel can be conducted. This paper summarizes characteristics of ideal draw solutes, recovery method of the draw solutes, and various application examples.

• KEPCO Journal on Electric Power and Energy
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• v.6 no.2
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• pp.173-179
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• 2020

Electric vehicles (EVs) using lithium secondary batteries (LIBs) with excellent power and long-term cycle performance are gaining interest as the successors of internal combustion engine (ICE) vehicles. However, there are few systematic researches for fast charging to satisfy customers' needs. In this study, we compare the degradation of LIB where its composition is LiNi_0.5Co_0.2Mn_0.3/Graphite with the constant current and constant power-charging method. The charging speed was set to 1C, 2C, 3C and 4C in the constant current mode and the value of constant power was calculated based on the energy at each charging speed. Therefore, by analyzing the battery degradation based on the same charging energy but different charging method; CP charging method can slow down the battery degradation at a high rate of 3C through the voltage curve, capacity retention and DC-IR. However, when the charging rate was increased by 4C or more, the deviation between the LIBs dominated the degradation than the charging method.

• The KIPS Transactions:PartD
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• v.10D no.6
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• pp.1059-1066
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• 2003

In this paper, the magnetic sensing system is designed and implemented for the safe security in internet commerce system. When the payment is required inthe internet commerce system, the magnetic sensing system will get the information from a credit card without keyboard input and then encrypt and transmit the information to server. The credit card sensing system, which is proposed in this paper, is safe from keyboard hacking because it encrypts card information immediately in its internal chip and sends the information to host system. For the protection of information, the magnetic sensing system is basically based on a synchronous stream cipher cryptosystem which is related to a group of matrices. The size of matrices and the bits of keys for the best performances are determined for various cases. It is shown that for credit card payments. matrices of size 2 have good performance even at most 128bits keys with the consideration of inverse matrices. For authentication of general-purpose data, the magnetic sensing system needs more than 1.5KB data and in this case, the optimum size of matrices is 2 or 3 at more 256bits keys with consideration of inverse matrices.

• The Journal of the Korea Contents Association
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• v.10 no.1
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• pp.295-304
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• 2010

This study aims to be of help to cope with internal and external circumstances of hospital effectively by analyzing the weight of role for a hospital manager which has been changing. It surveyed 191 top managers and administrators working in a medical institute like hospital located in Busan, Ulsan and Kyungnam area from 20 November, 2007 to 29 February, 2008. The results of this study were as follows: The top manager thought that the role as a leader is the most important role as a manager in present, but the role as an enterpriser is also very important in future, however, they had troubles in the role of a representative which they have carried out in present. In order to overcome the current medical situation that competition is getting keen, it seemed that they expected the role as an enterpriser would be more important in future for competition and organization improvement. The administrator thought the role as a leader is the most important in present, in future and in current performance. It was considered that they recognized the importance of team work, order, goal of the organization, strategies, and information interchange regarding the characteristics of hospital that there are people of various kinds of occupations.

• Journal of the Korean association of regional geographers
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• v.22 no.1
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• pp.251-266
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• 2016

Intelligent interpolation methods such as dasymetric mapping are considered to be the best way to disaggregate zone-based population data by observing and utilizing the internal variation within each source zone. This research reviews the advantages and problems of the dasymetric mapping method, and presents a geographically weighted regression (GWR) based method to take into consideration the spatial heterogeneity of population density - land cover relationship. The locally adaptive intelligent interpolation method is able to make use of readily available ancillary information in the public domain without the need for additional data processing. In the case study, we use the preclassified National Land Cover Dataset 2011 to test the performance of the proposed method (i.e. the GWR-based multi-class dasymetric method) compared to four other popular population estimation methods (i.e. areal weighting interpolation, pycnophylactic interpolation, binary dasymetric method, and globally fitted ordinary least squares (OLS) based multi-class dasymetric method). The GWR-based multi-class dasymetric method outperforms all other methods. It is attributed to the fact that spatial heterogeneity is accounted for in the process of determining density parameters for land cover classes.

• Journal of Microbiology and Biotechnology
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• v.20 no.10
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• pp.1450-1456
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• 2010

Accurate and rapid diagnosis of Pandemic Influenza A/H1N1 2009 virus (H1N1 2009) infection is important for the prevention and control of influenza epidemics and the timely initiation of antiviral treatment. This study was conducted to evaluate the performance of several diagnostic tools for the detection of H1N1 2009. Flocked nasopharyngeal swabs were collected from 254 outpatients of suspected H1N1 2009 during October 2009. This study analyzed the performances of the RealTime Ready Inf A/H1N1 Detection Set (Roche), Influenza A (H1N1) Real-Time Detection Kit (Bionote), Seeplex Influenza A/B OneStep Typing Set [Seeplex Reverse Transcriptase PCR (RT-PCR)], BinaxNow Influenza A & B Test Kit [Binax Rapid Antigen Test (RAT)], and SD BIOLINE Influenza Ag kit (SD RAT). Roche and Bionote real-time RT-PCR showed identical results for the H1N1 2009 hemagglutinin gene. Compared with real-time RT-PCR, the sensitivities and specificities were 83.7% and 100% for Seeplex RT-PCR, 64.5% and 94.7% for Binax RAT, and 69.5% and 100% for SD RAT. The sensitivities of Seeplex RT-PCR, Binax RAT, and SD RAT in patients aged over 21 years were 73.7%, 47.4%, and 57.9%, respectively. The sensitivities of Seeplex RT-PCR, Binax RAT, and SD RAT on the day of initial symptoms were mostly lower (68.8%, 56.3%, and 31.3%, respectively). In conclusion, multiplex RT-PCR and RAT for the detection of H1N1 2009 were significantly less sensitive than real-time RT-PCR. Moreover, a negative RAT may require more sensitive confirmatory assays, because it cannot be ruled out from influenza infection.

• Journal of Convergence for Information Technology
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• v.10 no.11
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• pp.324-331
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• 2020

This study used the financial information of 29 Regional public hospitals registered in the HASPA. Regional public hospitals analyzed the ratio of operating financing by management activities and how they affect net profit. The results of the study, Regional Public Hospitals ratio of fund financing for management activities was 83.50 percent for medical revenues, 9.53 percent for Incidental Revenue of Medical, 4.54 percent for Contribution Revenue, 4.42 percent for Other non-medical profits, 1.21 percent for depreciation Expense and 0.73 percent for Amount of reserve for unique purpose project. As a result of examining the effect of financing by management operation on net profit, the ratio of operating funds by medical revenue, by incidental revenue of medical, by contribution revenue and by other non-medical profits had a positive impact on net profit of Regional public hospitals. And It has been confirmed that the ratio of amount of reserve for unique purpose project has a negative impact on net profit. Therefore, it is necessary for Regional public hospitals to manage costs in consideration of revenues and internal reserves.