• ALGAE
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• v.31 no.2
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• pp.167-174
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• 2016

Biological response of cells to variable conditions should affect the expression level of certain genes. Quantification of these changes in target genes needs stable internal controls. Real-time quantitative polymerase chain reaction (PCR) has traditionally used reference or ‘housekeeping’ genes, that are considered to maintain equal expression in different conditions, to evaluate changes in target genes between samples and experimental conditions. Recent studies showed that some housekeeping genes may vary considerably in certain biological samples. This has not been evaluated in red algae. In order to identify the optimal internal controls for real-time PCR, we studied the expression of eleven commonly used housekeeping genes; elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, β-actin, polyubiquitin, 30S ribosomal gene, 60S ribosomal gene, beta-tubulin, alpha-tubulin, translation initiation factor, ubiquitin-conjugating enzyme, and isocitrate dehydrogenase in different life-history stages of Bostrychia moritziana. Our results suggest that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 30S ribosomal gene, have the most stable gene expression levels between the different life history stages (male, female, carposporophyte, and tetrasporophyte), while the other genes are not satisfactory as internal controls. These results suggest that the combinations of GAPDH and 30S would be useful as internal controls to assess expression level changes in genes that may control different physiological processes in this organism or that may change in different life history stages. These results may also be useful in other red algal systems.

• Journal of Korean Society on Water Environment
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• v.25 no.6
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• pp.922-934
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• 2009

The transport of contaminants and spatial variation in a deep reservoir are certainly governed by the thermal structure of the reservoir. There has been continuous efforts to utilize three-dimensional (3D) hydrodynamic and water quality models for supporting reservoir management, but the efforts to validate the models performance using extensive field data were rare. The study was aimed to evaluate a 3D hydrodynamic model, ELCOM, in Daecheong Reservoir for simulating heat fluxes and stratification processes under hydrological years of 2001, 2006, 2008, and to assess the impact of internal wave on the reservoir mixing. The model showed satisfactory performance in simulating the water temperature profiles: the absolute mean errors at R3 (Hoenam) and R4 (Dam) sites were in the range of $1.38{\sim}1.682^{\circ}C$. The evaporative and sensible heat losses through the reservoir surface were maximum during August and January, respectively. The net heat flux ($H_n$) was positive from February to September, while the stratification formed from May and continued until September. Instant vertical mixing was observed in the reservoir during strong wind events at R4, and the model reasonably reproduced the mixing events. A digital low-pass filter and zero crossing method was used to evaluate the potential impact of wind-driven internal wave on the reservoir mixing. The results indicated that most of the wind events occurred in 2001, 2006, 2008 were not enough to develop persistent internal wave and effective mixing in the reservoir. ELCOM is a suitable 3D model for supporting water quality management of the deep and stratified reservoirs.

• Journal of Radiation Protection and Research
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• v.38 no.4
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• pp.234-236
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• 2013

Some of radioactive contamination is unavoidable in the facilities using the unsealed radioactive material. The primary purpose of radioactive contamination control in the workplace with contamination concern is the effects from the potential intake of radioactive material into the body. This paper provides procedures to estimate the level of internal exposure for the worker based on the conservative assumptions and simple calculations. They consist of two processes; to calculate air concentration of radioactive material and annual intake by inhalation with contaminated air and to compare each of them to Derived Air Concentration and Annual Limit on Intake mentioned in the related notification. The procedures are applicable to make a decision on practical requirements for monitoring air contamination and internal exposure of worker as follows; needs for measurement of air contamination and internal exposure and acquisition of information on the design of the ventilation system.

• The Transactions of the Korea Information Processing Society
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• v.3 no.2
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• pp.251-266
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• 1996

In this paper, we design MHEG engine that can generate MHEG objects and present It to the users in Multimedia/Hypermedia Applications In the MHEG engine, the transmitted MHEG objects decoded into internal format. For the easy interpretation of MHEG objects, we define internal format as to be matched for each MHEG object. We easily processobjectinformation using the tree data structure because object inheritance and possession can be represented in tree structure. Object inheritance and possession must be represented in the internal format because they used in resolving the reference to external objector data file. The presentation synchronization extracts the synchronization information from MHEG composite objects, representing and controlling heterogencous media associated to spatio- temporal relation. In order to exactly represent the spatio-temporal synchronization included into MHEG composite object, we propose the algorithm that processes synchronization using the message of the synchronization module and the internal objects. MHEG engine proposed in this paper may be basic technology fro multimedia application area using Korea New Net.

• International Journal of Computer Science & Network Security
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• v.22 no.6
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• pp.109-114
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• 2022

The article is devoted to analyzing the police influence on road safety formation. The globalization processes confirm the study's relevance, provoking population mobility and the need to increase freight traffic. The study aims to identify the factors that affect road safety and the activities of the police and the EU transport safety units. An empirical analysis using factor analysis, correlation analysis, and general scientific methods of cognition were carried out to achieve the goal. The analysis results found that the number of police officers affects road safety, but not in all countries, which confirms the importance of other factors. Based on the analysis results of the scientific literature, the factors affecting transport safety are legislative regulation, the use of innovative technologies, transport infrastructure, geography, and psycho-physical and emotional drivers factors. It has been proved that the police authorities and units cannot fully ensure transport safety in the European Union because the safety is formed by a complex of actions by the state and road users.

• The Journal of Internal Korean Medicine
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• v.21 no.1
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• pp.65-73
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• 2000

Objectives : The effects of cotreatment of adriamycin and ethanol extract of herb (Palgin-tang hab Hwajuck-hwan a traditional medicine for cancer treatment in oriental medicine) on the induction of apoptotic cell death were investigated in human liver origin cell lines, Chang. Methods : Chang(ATCC) liver cells were cultured in RPMI-1640(Gibco SRL Co, Gaithersburg, MD) badge including 10% fetal bovine serum. Chang liver cells were treated with various concentrations(from 10 to $0.16{\mu}l$) of adriamycin and herb extract(from 500 to $31.25{\mu}l$) After 48h later, the cells were tested for viability by Crystal violet staining assay. Adriamycin and Herb extract induced ladder pattern of DNA fragmentation in Chang cells. Genomic DNA was isolated and separated on 1.5% agarose gels. The DNA was stained with ethidium bromide and visualized under UV light. Results : The death of Chang cells was synergistically induced by the cotreatment of adriamycin and ethanol extract of herb. In addition, the cotreatment-induced cell death of Chang cells was mediated by apoptotic death signal processes. The phosphotransferase activity of JNK1 remained in a basal level in Chang cells which was treated individually with the adriamycin and ethanol extract of herb. However, it was markedly increased in Chang cells which was cotreated with adriamycin and ethanol extract of herb. In addition, the expression of Fas and FasL was markedly induced by the cotreatment of adriamycin and herb extract. For a while, the expression of Sax was a eminently increased by the ethanol extract of herb. However, Scl2 expression was not affected by the individual or cotreatment of adriamycin and herb extract. Conclusions : our results suggest that the cotreatment of adriamycin aM ethanol extract of herb induces synergistic apoptotis of human liver origin Chang cells via the upregulation of JNK, Fas, FasL and Bax.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.2
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• pp.58.1-59
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• 2021

Exploration of asteroids' internal structure is essential for understanding their evolutional history. It also provides a fundamental information about the history of coalescence and collision of the solar system. Among several models of the internal structures, the rubble-pile model, confirmed by the near-Earth asteroid (25143) Itokawa by Hayabusa mission [1], is now widely regarded as the most common to asteroids with size ranging from 200 m to 10 km [2]. On the contrary, monolithic and core-mantle structures are also possible for small asteroids [3]. It is, however, still challenging to look through the interior of a target object using remote-sensing devices. In this presentation, we introduce our ongoing research conducted at Seoul National and propose an idea to infer the internal structure of Apophis using available instruments. Itokawa's research provides an important benchmark for Apophis exploration because both asteroids have similar size and composition [4][5]. We have conducted research on Itokawa's evolution in terms of collision and space weathering. Space weathering is the surface alteration process caused by solar wind implantation and micrometeorite bombardment [6]. Meanwhile, resurfacing via a collision acts as a counter-process of space weathering by exposing fresh materials under the matured layer and lower the overall degree of space weathering. Therefore, the balance of these two processes determine the space weathering degrees of the asteroid. We focus on the impact evidence on the boulder surface and found that space weathering progresses in only 100-10,000 years and modifies the surface optical properties (Jin & Ishiguro, KAS 2020 Fall Meeting). It is important to note that the timescale is significantly shorter than the Itokawa's age, suggesting that the asteroid can be totally processed by space weathering. Accordingly, our result triggers a further discussion about why Itokawa indicates a moderately fresh spectrum (Sq-type denotes less matured than S-type). For example, Itokawa's smooth terrains show a weaker degree of space weathering than other S-type asteroids [7]. We conjecture that the global seismic shaking caused by collisions with >1 mm-sized interplanetary dust particles induces granular convection, which hinders the progression of space weathering [8]. Note that the efficiency of seismic wave propagation is strongly dependent on the internal structure of the asteroid. Finally, we consider possible approaches to investigate Apophis's internal structure. The first idea is studying the space weathering age, as conducted for Itokawa. If Apophis indicates a younger age, the internal structure would have more voids [9]. In addition, the 2029 close encounter with Earth provides a rare natural opportunity to witness the contrast between before and after the event. If the asteroid exhibits a slight change in shape and space weathering degree, one can determine the physical structure of the internal materials (e.g., rubble-pile monolithic, thick or thin regolith layer, the cohesion of the materials). We will also consider a possible science using a seismometer.

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.14 no.2
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• pp.135-146
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• 2019

Objectives : There are many theories about the anatomical relationships between the thoracic spinous and transverse processes. However, these studies were all conducted on cadavers. Thus, there might be differences when applied to living individuals. Therefore, the aim of this study was to determine whether the theories were similar when measured in living individuals. Methods : We conducted studies with 10 individuals aged 20-30 using 3D-CT. Four different measurements were taken between the thoracic spinous and transverse processes. To facilitate the relationship analysis, the spinous process positioned between the traverse process of the segment and the lower segment was set to 0.5. The spinous process located at the height of the traverse process of the lower segment was set to 1. The spinous process located between the traverse process of the lower segment and the two segments below was set to 1.5. Results : Therefore, based on the aforementioned settings, T1-T4 were 1, T5-T7 were 1.5, T8-T10 were 1, and T11 and T12 were 0.5. This indicated that the spinous processes of T1-T4 and T8-T10 were equal in height to the transverse processes of the lower segment, and that T5-T7 were at the midpoint of the height of the transverse processes between the lower segment and two segments below, and T11, T12 corresponded to the midpoint between the traverse process of the segment and the lower segment. Conclusions : Neither 'Geelhoed's rule'nor 'the rule of three'was accurate when applied to living individuals. However, this study had some limitations, so further research is needed.

• Economic and Environmental Geology
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• v.49 no.1
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• pp.43-52
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• 2016

The essential role of the Division of Earth Sciences(EAR) in the Directorate of Geoscience(GEO) of National Science Foundation of America(NSF) is to support basic research aimed at acquiring fundamental knowledge of the Earth system that can be directly applied to the United States' strategic needs. The 2011 Committee on New Research Opportunities in the Earth Sciences(NROES) of the National Academy of Sciences(NAS) identified specific areas of the basic earth science research scope of the EAR that were poised for rapid progress during the next decade. Quantified by interdisciplinary approaches, the Committee highlighted the following topics relating to the EAR Deep Earth Processes and Surface Earth Processes sections: (1) the early Earth; (2) thermochemical internal dynamics and volatile distribution; (3) faulting and deformation processes; (4) interactions among climate, the Earth surface processes, tectonics, and deep Earth processes; (5) co-evolution of life, environment, and climate; (6) coupled hydrogeomorphic-ecosystem response to natural and anthropogenic change; and (7) interactions of biogeochemical and water cycles in terrestrial environments. We also promote future research challenges such as the critical zone studies. In order to promote more active such a huge future research challenges, additional research support policies are needed.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2003.09a
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• pp.439-442
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• 2003

In order to solve the difficulties of parameter settings in SA algorithm, an improved practical SA algorithm is proposed by employing the threading techniques, appropriate software structures, and dynamic adjustments of temperature parameters. Threads provide a mechanism to realize a parallel processing under a disperse environment by controlling the flux of internal information of an application. Thread services divide a process by multiple processes leading to parallel processing of information to access common data. Therefore, efficient search is achieved by multiple search processes, different initial conditions, and automatic temperature adjustments. The proposed are methods are evaluated, for three types of Traveling Salesman Problem (TSP) (random-tour, fractal-tour, and TSPLIB test data)are used for the performance evaluation. The experimental results show that the computational time is 5％ decreased comparing to conventional SA algorithm, furthermore there is no need for manual parameter settings. These results also demonstrate that the proposed method is applicable to real-world vehicle routing problems.