• BMB Reports
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• 제51권1호
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• pp.1-2
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• 2018

Positive selection on a new beneficial mutation generates a characteristic pattern of DNA sequence polymorphism when it reaches an intermediate allele frequency. On genome sequences of African Drosophila melanogaster, we detected such signatures of selection at 37 candidate loci and identified "sweeping haplotypes (SHs)" that are increasing or have increased rapidly in frequency due to hitchhiking. Based on geographic distribution of SH frequencies, we could infer whether selective sweeps occurred starting from de novo beneficial mutants under simple constant selective pressure. Single SHs were identified at more than half of loci. However, at many other loci, we observed multiple independent SHs, implying soft selective sweeps due to a high beneficial mutation rate or parallel evolution across space. Interestingly, SH frequencies were intermediate across multiple populations at about a quarter of the loci despite relatively low migration rates inferred between African populations. This invokes a certain form of frequency-dependent selection such as heterozygote advantage. At one locus, we observed a complex pattern of multiple independent that was compatible with recurrent frequency-dependent positive selection on new variants. In conclusion, genomic patterns of positive selection are very diverse, with equal contributions of hard and soft sweeps and a surprisingly large proportion of frequency-dependent selection in D. melanogaster populations.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1434-1440
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• 2006

The stable anchorage of pyridoxal 5'-phosphate (PLP) in the active site of D-amino acid transaminase (D-AT) is crucial for the enzyme catalysis. The three-dimensional structure of D-AT revealed that Glu32 is one of the active site groups that may playa role in PLP binding. To prove the role of Glu32 in PLP stability, we firstly checked the rate of the potential rate-limiting step. The kinetic analysis showed that the rate of the ${\alpha}$-deprotonation step reduced to 26-folds in E32A mutant enzyme. Spectral analyses of the reaction of D-AT with D-serine revealed that the E32A mutant enzyme failed to stabilize the key enzyme-substrate intermediate, namely a quinonoid intermediate (E-Q). Finally, analysis of circular dichroism (CD) on the wild-type and E32A mutant enzymes showed that the optical activity of PLP in the enzyme active site was lost by the removal of the carboxylic group, proving that Glu32 is indeed involved in the cofactor anchorage. The results suggested that the electrostatic interaction network through the groups from PLP, Glu32, His47, and Arg50, which was observed from the three-dimensional structure of the enzyme, plays a crucial role in the stable anchorage of the cofactor to give necessary torsion to the plane of the cofactor-substrate complex.

• 공업화학
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• 제18권5호
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• pp.433-437
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• 2007

본 연구에서는 기능성 근적외선 흡수색소 및 광안정제로 사용되는 bis(dithiobenzil) 금속 화합물은 중간체인 benzoin 및 anisoin을 먼저 합성하고 이들을 전구체로 사용하여 합성하였다. 또한, bis(dithiobenzil) 금속 화합물의 광안정성 효과를 확인하기 위하여 전하발생재료인 squarylium을 합성하였다. 중간체 및 최종 생성물의 구조는 핵자기 공명 분석기와 적외선 분광기를 이용하여 확인하였고, 열적 특성은 시차열분석기 및 열중량분석기를 이용하여 분석하였다. 광학 특성과 광안정성 효과는 UV-Vis-NIR 분광기를 이용하여 측정 분석하였다. 합성한 bis(dithiobenzil) 금속 화합물은 근적외선 영역에서 우수한 흡광특성을 나타내었고, 치환기의 종류 및 위치에 따라 최대흡수파장이 이동하였다. 또한, squarylium에 bis(dithiobenzil) 금속 화합물을 첨가하면 squarylium의 광퇴색현상을 감소시켰다.

• Bulletin of the Korean Chemical Society
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• 제15권10호
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• pp.857-860
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• 1994

We have calculated the ${\pi}$-electron density, atom self-polarizability, and free valence on each atom of N-(2-chlorobenzyl)-pyridinium, N-(benzyl)-2-chloropyridinium, and N-(2-chlorobenzyl)-2-chloropyridinium salts using a simple Huckel method in order to discuss their intramolecular photocyclization reaction in a qualitative method. Our calculation qualitatively predicts that photocyclization occurs through forming radicals as a reaction intermediate by breaking a C-Cl bond after photoexcitation into a triplet state via intersystem crossing from an initially excited singlet state. We noticed that this C-Cl bond breaking is aided by ${\pi}$-complex formation between a chlorine atom and the ${\pi}$ -electrons of the neighboring ring in the triplet state and a stronger ${\pi}$-complex bond makes C-Cl bond breaking, i.e., radical formation, much easier. A chlorine atom will form a stronger ${\pi}$ -complex bond to a benzyl ring of N-(benzyl)-2-chloropyridinium than a pyridinium ring of N-(2-chlorobenzyl)-pyridinium because the former can donate its ${\pi}$-electron more easily than the latter. The chlorine at position 15 of N-(2-chlorobenzyl)-2-chloropyridinium salt in the excited state also provides its ${\pi}$-electron to the benzyl ring. So this ${\pi}$-electron can increase the bond strength of the $\pi-complex.$ Therefore, the strength of ${\pi}$-complex follows the order of N-(2-chlorobenzyl)-2-chloropyridinium, N-(benzyl)-2-chloropyridinium, and N-(2-chlorobenzyl)-pyridinium salts and thus the radical formation rate. This provides us with an intramolecular photocyclization reaction rate of the same order as given above.

• 한국건설관리학회논문집
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• 제20권6호
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• pp.117-125
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• 2019

본 연구에서는 해외 산업단지 건설의 국내경제 파급효과를 극대화하기 위한 방안을 도출하는데 목적이 있다. 이를 위해 해외 건설협회의 준공실적 원시자료와 산업연관모형을 응용하여 산업단지 개발단계별(조사설계, 단지조성, 공장건축) 유발계수를 도출하였다. 분석결과, 도시용역은 인건비 비중이 높게 나타나고 있으며, 단지토목은 손익액 비중이 높게 나타났다. 이에 비해 공장건축은 중간투입비중과 손익비중이 높게 나타났다. 결국 동일한 공종과 비교할 때 산업단지의 해외개발은 고용측면에서는 용역이, 기업의 영업잉여 측면에서는 토목과 건축이, 국가경제 측면에서는 건축의 효과가 크게 나타난다. 따라서 우리나라 국내경제 파급 효과를 극대화하기 위해서는 단순히 해외 산업단지 개발을 통해 부지를 분양하는 사업구조보다는 산업단지 내 공장 건축까지 사업에 포함할 수 있는 수주전략을 도모할 필요가 있음을 시사한다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.260-260
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• 1994

Mammalian pyruvate dehydrogenase complex(PDC) enzyme consists of multiple oopies of three major oligomeric enzymes-El, E2 E3. And protein X is one of the enzymatic constituents which is tightly bound to E2 subunit This complex enzyme is responsible for the oxidative decarboxylation of pyruvate producing of acetyl CoA which is a key intermediate for the entry of carbohydrates into the TCA cycle for its complete metabolic conversion to CO$_2$. And the overall activity of the complex enzyme is regulated via covalent nodification of El subunit by a El specific phosphatase ad kinase. Protein X has lipoyl moiety that undergoes reduction and acetylation during ezymatic reaction and has been known h be involved in the binding of E3 subunit to E2 core and in the regulatory activity of kinase. The purification of protein X has not been achieved majorly because of its tight binding to E2 subunit The E2-protein X subcomplex was obtained by the established methods and the detachment of protein X from E2 was accomplished in the 0.1M borate buffer containing 150mM NaCl. During the storage of the subcomplex in frozen state at -70$^{\circ}C$, the E2 subunit was precipitated and the dissociated protein X was obtained by cntrifegation into the supernatant The verification of protein X was accomplished by (1)the migration on SDS-PAGE, (2)acetylation by 〔2$\^$-l4/C〕 pyruvate, and (3)internal amino acid sequence analysis of tryptic digested enzyme.

• 한국주거학회논문집
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• 제24권1호
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• pp.33-40
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• 2013

This study has examined layout of communal outdoor space and welfare facilities in the nation's very first senior apartment complex in Segok-dong, Gangnam-gu that has been developed by Seoul City. In order for this space to play a role as a center of communication: 1) A center space of community should be created at the point of intersection of main moving lines of pedestrians. 2) Hierarchical area should be set up between main moving lines of walking and communal outdoor space. 3) It should be designed in a way to reflect demands of seniors and be accommodated their activities and behaviors. 4) It should be functionally connected to local infrastructure facilities so that it can promote social exchanges among seniors. Points to be considered in welfare facilities: 1) They should be placed at the center of the complex to provide convenience to all seniors. 2) Area of senior citizen center should be subject to flexible legal regulation in light of the unique characteristics of the Complex. 3) in case that welfare facilities are installed in the same space, intermediate space is required to alleviate collision between private space and public space. 4) Senior assistance facilities should put more emphasis on running programs that make good use of the space than on securing the space itself.

• Bulletin of the Korean Chemical Society
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• 제33권7호
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• pp.2213-2218
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• 2012

Efficient synthetic routes were developed to prepare a sizable amount (4-15 grams) of the chiral epoxides 4-6 as versatile intermediates for the synthesis of aspartyl protease inhibitors of therapeutic interest such as HIV protease and ${\beta}$-secretase. Oxidative cleavage of the C(2)-C(3) double bond of L-ascorbic acid followed by functional group manipulation led to the preparation of the epoxide 10, which was opened with an azide to yield a common aziridine intermediate 12. Through opening of the aziridine ring of 12 with either a carbon or a sulfur nucleophile, chiral epoxide precursors 4-6 could be prepared for various HIV protease inhibitors. Except for the final low melting epoxides 5 and 6, all intermediates were obtained as crystalline solids, thus the synthetic pathway can be easily applied to a large-scale synthesis of the chiral epoxides.

• 한국지반신소재학회논문집
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• 제21권3호
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• pp.11-19
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• 2022

최근 도시화 및 경제 성장에 따라 기피시설로 인식되는 소각장, 매립지와 같은 지상 환경 기초 시설의 지하화에 대한 요구가 증대하고 있다. 대규모 지하 복합플랜트 건설을 위해서는 다양한 지반 조건 및 하중 조건에 따른 안정성 검토가 필요하다. 본 논문에서는 유한요소해석(FEM)을 활용하여 지하 대공간 상부하중과 수평토압을 고려한 지하연속벽체 건설 공정에 따른 수평, 연직 변위 및 응력 발생 경향을 분석하였다. 다양한 조건의 해석 결과를 바탕으로 지하 복합플랜트 상세설계 및 시공단계에서 고려해야 할 요소들을 검토하고, 지하 대공간 건설을 위한 설계인자(중간벽체 설치 유무, 사전 보강 영역 등)에 관한 시사점을 도출하였다.

• The Korean Journal of Pain
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• 제35권3호
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• pp.319-326
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• 2022

Background: As the internet usage becomes easily accessible, the patients are more frequently searching about diseases and medical/non-medical treatments. Considering that complex regional pain syndrome (CRPS) is a debilitating disease, it is important to check the information that patients are accessing. Therefore, this study aimed to investigate the reliability, sufficiency, and accuracy of the YouTube videos about CRPS. Methods: This study is a descriptive research which is derived by searching videos using the keyword 'complex regional pain syndrome' on YouTube. Relevance-based sequencing was used to sort the videos. Sources and video parameters were documented. To evaluate the accuracy, reliability and content quality of the videos, Global Quality Score, Journal of American Medical Association Benchmark Criteria and Modified DISCERN Questionnaire scales were used. Results: A total of 167 videos were included in this study. The majority of the videos originated from USA (80.2%, n = 134). The median number of views was 639 and the viewing rate was 73.3. Most of the videos had partially sufficient data and the interaction index viewing rate parameters for videos with high content quality were greater than videos with low content quality (P = 0.010, P = 0.014). Conclusions: Our results showed that videos about CRPS on YouTube mostly had partially sufficient data and include intermediate-high quality contents. Moreover, high-content quality videos had higher viewing rates, interaction indexes, number of likes, longer durations, as well as better reliability and accuracy scores. Videos with high quality and reliable content are needed to reduce misinformation about CRPS.