• Title/Summary/Keyword: Intermediate Technology

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Distributions of Water Temperature and Salinity in the Korea Southern Coastal Water During Cochlodinium polykrikoides Blooms (C. polykrikoides 적조 발생시의 한국 남해안의 수온 및 염분 분포)

  • Lee, Moon-Ock;Choi, Jae-Hoon
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.12 no.4
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    • pp.235-247
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    • 2009
  • In order to elucidate the cause of Cochlodinium polykrikoides blooms in the Korea southern coastal water, we investigated observational data of water temperatures and salinities in summer and winter, obtained from the stoppage of ship by NFRDI (National Fisheries Research and Development Institute) as well as composite images by NOAA from 1995 to 2008. Cochlodinium polykrikoides blooms occurred when water temperature was approximately $25.0{\sim}26.0^{\circ}C$ and salinity was 31.00 psu on average in Narodo neighboring seas. Different thermohaline fronts were observed between the Korea southern coastal water and the open sea water in summer and winter, respectively. That is, in winter four fronts were observed between the Korea southern coastal water with low temperature and low salinity, intermediate water originated from Tsushima Warm Current, Tsushima Warm Current with high temperature and high salinity, and the China coastal water with low temperature and low salinity. In contrast, in summer two fronts were observed between the Korea southern coastal water with low temperature and high salinity, Tsushima Warm Current with high temperature and low salinity, and the China coastal water with high temperature and high salinity. These thermohaline fronts also proved to be formed by two water masses with a different physical property, in terms of T-S diagrams. Consequently, we noticed that C. polykrikoides blooms occurring in Narodo neighboring seas in summer had a close relationship with thermohaline fronts observed between the Korea southern coastal water and Tsushima Warm Current.

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The Effect of Root Exudate and Chemotaxis on Host Recognition in Soybean-Bradyrhizobium Symbiosis (대두(大豆)-근류균(根瘤菌) 공생(共生)에서 뿌리분비물(分泌物)과 화학주성(化學走性)이 숙주인식(宿主認識)에 미치는 영향(影響))

  • Kang, Sang Jai;Park, Woo Churl
    • Current Research on Agriculture and Life Sciences
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    • v.11
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    • pp.121-132
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    • 1993
  • This study was carried out to research the effect of the chemotaxis of Bradyrhizobium japonicum KCTC 2422 and its mutant toward soybean root exudate and to elucidate the effect of the lectin of host specificity (Host Recognition) in soybean-Bradyrhizobium symbiosis. The results obtained were as follows: The homogeneities of the purified lectins from soybean and pea seed was ascertained chromatographically and electrophoretically. Gel electrophoresis of soybean lectin in the presence of sodium dodecyl sulfate appeared a single protein band, whereas pea lectin appeared two protein bands. Soybean lectin from 2 cultivars formed immunoprecipitin arcs at same position with anti-soybean lectin rabbit IgG, but pea lectin did not form immunoprecipitin lines with anti-soybean lectin rabbit IgG. Chemotactic responses of KCTC 2422, LPN-100 and LCR-101 toward proline in capillary assays were 3.1, 1.3 and 1.0-fold above background, respectively. The chemotactic responses of KCTC 2422, LPN-100, and LCR-101 toward Paldal crude root exudate in capillary assays were 3.5, 1.4 and 1.4-fold above background, respectively. The present work shows that B. japonicum and its mutants are capable of very different responses toward root exudate fraction. The chemotactic responses of KCTC 2422 was most with neutral fraction, least with anionic fraction and intermediate with cationic fraction. The nitrogenase activity of soybean nodule was shown in 15days after inoculation with LCR-101. However, we couldn't find out the nodules when soybean was inoculated with LPN-100. From these result we can suppose that the chemotaxis of Bradyrhizobium plays inportant the role of forming the nodule (host recognition) in the soybean-B. japonicum symbiosis.

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Impact of CYP2D6 Polymorphisms on Tamoxifen Responses of Women with Breast Cancer: A Microarray-based Study in Thailand

  • Sukasem, Chonlaphat;Sirachainan, Ekaphop;Chamnanphon, Montri;Pechatanan, Khunthong;Sirisinha, Thitiya;Ativitavas, Touch;Panvichian, Ravat;Ratanatharathorn, Vorachai;Trachu, Narumol;Chantratita, Wasun
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.9
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    • pp.4549-4553
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    • 2012
  • This study was designed to investigate the frequency of CYP2D6 polymorphisms and evaluate the association between genetic polymorphisms of CYP2D6 and tamoxifen therapeutic outcome in Thai breast cancer patients. We recruited 48 breast cancer patients who received adjuvant tamoxifen for evaluating CYP2D6 genetic polymorphisms using microarray-based technology. Associations between genotypes-phenotypes and disease free survival were analyzed. Median follow up time was 5.6 years. The mean age of the subjects was 50 years. The 3 common allelic frequencies were 43.8% ($^*10$), 36.5 ($^*1$) and 10.4% ($^*2$) which are related to extensive metabolizer (EM) and intermediate metabolizer (IM) with 70.8% and 29.2 %, respectively. No association between CYP2D6 genotypes and DFS was demonstrated. Nevertheless, exploratory analysis showed statistically significant shorter DFS in the IM group of post-menopause patients (HR, 6.85; 95%CI, 1.48-31.69; P=0.005). Furthermore, we observed statistically significant shorter DFS of homozygous $CYP2D6^*10$ when compared with heterozygous CYP2D6*10 and other genotypes (P=0.005). $CYP2D6^*10$ was the most common genotype in our subjects. Post-menopause patients with homozygous $CYP2D6^*10$ and IM have shorter DFS. To confirm this relationship, larger samples and comprehensively designed trials in Thailand are required.

Nucleophilic Substitutions at a Carbonyl Carbon Atom (ⅩⅡ). Solvolysis of Methylchloroformate and Its Thioanalogues in $CH_3CN-H_2O$ and $CH_3COCH_3-H_2$ Mixtures (카르보닐탄소원자의 친핵성 치환반응 (제 12 보).아세토니트릴-물 및 아세톤-물 혼합용 매속에서 메틸클로로훠메이트와 그 티오유도체들의 가용매분해반응에 관한 연구)

  • Sangmoo La;Kyeong Shin Koh;Ikchoon Lee
    • Journal of the Korean Chemical Society
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    • v.24 no.1
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    • pp.8-14
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    • 1980
  • Solvolysis rate constants for methylchloroformate, $CH_3O$(CO)Cl, methylthiono-chloroformate, $CH_3O$(CS)Cl, and methylthiolchloroformate, $CH_3S$(CO)Cl, have been determined conductometrically in acetone-water and acetonitrile-water mixtures, and activation parameters, ${\Delta}H^{\neq}$ and ${\Delta}S^{\neq}$, have been derived. Results show that in water-rich regions the order of rate increases as $$CH_3O(CO)Cl while in dipolar aprotic solvent-rich region this order reverses. The plots of log k vs. solvent parameters, Y, $\frac{D-1}{2D+1}$ and log($H_2$) show that the order of rate increase in water-rich region is the results of increase in $S_N1$ character. It is concluded that $CH_3S$(CO)Cl solvolyzes via $S_N1$ mechanism whereas $CH_3O$(CO)Cl reacts via $S_N2$ and $CH_3O$(CS)Cl via intermediate mechanism in water-rich region.

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Improved Model for Index of Construction Engineer's Competency Evaluation System in Domestic Construction Management (국내 건설사업관리 기술인력 역량평가 개선모델(I2CEC))

  • Kang, Seongmi;Cha, Minsu;Lee, Woojae;Ji, Woojong;Cho, Hunhee;Yoo, Wisung
    • Korean Journal of Construction Engineering and Management
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    • v.21 no.2
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    • pp.47-58
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    • 2020
  • The ICEC (Index of Construction Engineer's Competency) quantifies the competence of construction engineers using such parameters as experience, education, and qualifications and assigns four technical grades (expert, advanced, intermediate, and beginners) to construction engineers according to their scores for the efficient management and loading of technical personnel. However, as of 2020, the seventh year since its implementation, ICEC has shown many problems in its application, unlike its intended purpose. So institutional supplementation is required to provide improvement measures that can cope with the changing labor market environment and complement the current ICEC. Therefore, this study examined the current status of the career management system after the introduction of the ICEC, suggested a career index proportional to the competence of construction engineers from the beginner to the expert level, and developed an effective capability evaluation model I2CEC. The improved model presented in this study provides a means to comprehensively judge the performance, experience, and the professional work abilities of construction management engineers. Furthermore, the results of this study are expected to contribute to the development of efficient manpower and career management systems for enhancing the competitiveness of the domestic construction industry.

Effect of the processing variables on the formation of $Pb(Sc_{1/2}Nb_{1/2})O_3$ thin layers ($Pb(Sc_{1/2}Nb_{1/2})O_3$ 박막 형성에 미치는 공정변수의 영향)

  • Park, Kyung-Bong;Kwon, Seung-Hyeop;Kim, Tae-Huei
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.19 no.2
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    • pp.70-74
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    • 2009
  • Effect of the processing variables on the formation of $Pb(Sc_{1/2}Nb_{1/2})O_3$(hereafter PSN) thin layers prepared on Pt(111)/Ti/$SiO_2$/Si substrates using the sol-gel and the spin coating method has been studied. After each deposition, the coated films were heated at $370^{\circ}C$ for 5 min. Then they were finally sintered at temperature range of $600{\sim}700^{\circ}C$ by RTA(rapid thermal annealing). The final multilayered films showed a (111) preferred orientation. On a while, the layer-by-layer crystallization of multilayered amorphous thin films without the intermediate heating exhibited a (100) preferred orientation. In case of heat treatment in the tube furnace with the heating rate of $4^{\circ}C/min$, (100) and (111) oriented thin layers were formed simultaneously. The microstructure of the deposited films were dense and crack-free with thickness of 300nm, irrespective of the processing variables.

Convenient Evaluation of Stored Apple Pollen Viability by Fluorochromatic Reaction (형광염색반응에 의한 장기 저장 사과 화분의 활력 측정)

  • Lee, Hee Jae;Kim, Su-Jin;Kim, Tae-Choon
    • Horticultural Science & Technology
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    • v.17 no.3
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    • pp.337-340
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    • 1999
  • In order to evaluate stored apple pollen viability, in vitro germination test was performed on a microscope slide coated with the culture medium containing fluorescein diacetate (FDA). However, the inclusion of FDA to the culture medium declined pollen germination. Alternatively, the fluorochromatic reaction procedure was tested. The procedure involved dusting pollen grains onto drops of 10% sucrose solution containing 0.002% FDA and allowing them to accumulate fluorescein. Within 30 min after the fluorochromatic reaction, viable pollen grains clearly fluoresced under ultraviolet light. Both the in vitro germination test and the fluorochromatic reaction procedure revealed that stored apple pollen viability was not considerably decreased over storage up to at least 39 months. Of the cultivars examined by both methods, 'Fuji' and 'Senshu' pollen viability was highest, 'Tsugaru' was intermediate, and 'Jonagold' was lowest. The fluorescing percentages appeared approximately comparable to the germination percentages except for the 'Senshu' pollens stored for 3 months, although the fluorescing percentages was slightly higher than the germination percentages. Strong and highly significant correlations were found between the two methods. It can thus be concluded that the fluorochromatic reaction procedure provides a convenient and reliable evaluation of stored apple pollen viability.

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Selection and Characterization of Horticultural Traits of Tomato leaf curl virus (TYLCV)-resistant Tomato Cultivars (토마토 황화잎말림바이러스(TYLCV) 저항성 품종 선발 및 원예특성 분석)

  • Kim, Woo-Il;Kim, Kwang-Hwan;Kim, Young-Bong;Lee, Heung-Su;Shon, Gil-Man;Park, Young-Hoon
    • Horticultural Science & Technology
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    • v.31 no.3
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    • pp.328-336
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    • 2013
  • This study was conducted to evaluate imported tomato $F_1$ cultivars as breeding materials for the resistance to Tomato yellow leaf curl virus (TYLCV) by molecular markers and bioassay. From marker genotyping and disease evaluation of 40 $F_1$ cultivars, most of the cultivars declared as TYLCV-resistance carried heterozygous marker genotype for the TYLCV resistance genes Ty-1, Ty-3, or Ty-3a, and showed low disease rates. Whereas, 4 of 5 $F_1$ cultivars declared as intermediate resistance showed marker genotype for susceptibility and disease rates ranged 18.1-33.3%. However, the xx cultivars showed inconsistency in marker genotype and disease rate. Characterization of horticultural traits of the $F_1$ cultivars with TYLCV-resistance indicated that large-size fruit cultivars were higher in yield and similar in sugar contents and solid-acid ratio compared to a control cultivar preferred in the domestic market, although hardness remained to be a problem. On the other hand, cherry tomato cultivars showed lower yield and brix, but longer internode compared to a control cultivar, indicating that breeding for TYLCV-resistance using these cultivars will require more efforts and time compared to large-sized.

Dermatophytosis of the Four-toed Hedgehog Caused by Trichophyton erinacei

  • Yoon, Ji-Seon;Lee, Jong-Hyun;Yu, Do-Hyeon;Li, Ying-Hua;Lee, Mi-Jin;Iwasaki, T.;Park, Jin-Ho
    • Journal of Veterinary Clinics
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    • v.25 no.3
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    • pp.207-210
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    • 2008
  • Trichophyton erinacei is a dermatophyte pathogen that infects both humans and hedgehogs. A two-month old female four-toed hedgehog presented to the Chonbuk Animal Medical Center with pruritus, excoriation and crust on her face for ten days. The owner of the hedgehog also exhibited the clinical signs of scaly erythema with fine vesicles on her neck. A presumptive diagnosis of dermatophytosis was made based on the results of an acetate tape preparation in which hyphae and chains of arthroconidia were observed. The crusts from the lesions were then cultured on Sabouraud Dextrose Agar for identification. After 10 days of incubation, downy colored colonies that had a central umbo with a white granular surface and a yellow pigment ring in the reverse were observed. Microscopic analysis revealed the presence of numerous teardrop shaped microconidia singly attached to the sides of the hyphae. In addition, 2-6 roomed macroconidia that were somewhat irregular in shape and size were present, and abundant intermediate sized spores were observed between the micro and macro conidia. To confirm that the culture was T. erinacei, the internal transcribed spacer region of the 5.8S phase of the ribosomal RNA gene (ITS1-5.8S-ITS2 rDNA) was amplified by PCR and then sequenced. A 679-base pair fragment of DNA was then compared with sequences in GenBank and found to be 99% homologous with sequences of T. erinacei (Z97997 and Z97996. The clinical signs were resolved after four weeks of treatment with oral and topical ketoconazole and chlorhexidine. To the best of our knowledge, this represents the first case of T. erinacei isolated from a four-toed hedgehog in Korea.

A Study on Obesity, Eating-out Behaviors, and Eating Habits among the Housewives in Busan and Gyeongnam Province (부산.경남지역 주부들의 비만도와 외식행동 및 식생활습관에 관한 연구)

  • Lee, Bok-Seup;Park, Young-Hee;Lee, Jong-Ho
    • Culinary science and hospitality research
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    • v.17 no.3
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    • pp.151-161
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    • 2011
  • The aim of this study is to investigate the relationships among obesity, eating-out behaviors, and eating habits in the housewives living in Busan and Gyeongnam Province. In order to analyze the data obtained from 249 housewives, SPSS statistical program 17.0 was used. Also, frequency, factors, $x^2$-test, and cluster analyses were conducted as well as one-way ANOVA analysis. The results of the analyses have shown that their average height was 160.75 cm, and their average weight was 57.51 kg. Their BMI was investigated resulting in normal weight being 56.6%, overweight and obesity 37.0%. Concerning the number of eating out opportunities a month, 65.5% said under four times. Their per capita cost of eating out was between 7001 Won and 11,000 Won, being 34.9%. The purpose of eating out was family occasions being 44.2%. Those who were accompanied by them were their family members. and relatives being 63.8%. Their favorite restaurants were public restaurants being 47.8%. It was investigated that they got the information about eating-out through their acquaintances being 79.1%. According to the results of the cluster analysis by the factor of their eating habits, Cluster 1 was named as Intermediate Eating Habits group, Cluster 2 as Not Good Eating Habits group, and Ouster 3 as Good Eating Habits group. There were significant difference (P<0.01) in the difference analysis between the three clusters and the eating habits factor. This study showed that 37% of housewives in the Busan and Gyeongnam area were classified as overweight and obese and needed to change their eating behavior. The reason why obesity in the age group of those over 51 was higher than those under 30 was their going through menopause.

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