• Title/Summary/Keyword: Interaction Surface

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Characterization of the Surface Contribution to Fluorescence Correlation Spectroscopy Measurements

  • Chowdhury, Salina A.;Lim, Man-Ho
    • Bulletin of the Korean Chemical Society
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    • v.32 no.2
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    • pp.583-589
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    • 2011
  • Fluorescence correlation spectroscopy (FCS) is a sophisticated and an accurate analytical technique used to study the diffusion of molecules in a solution at the single-molecule level. FCS is strongly affected by many factors such as the stability of the excitation power, photochemical processes, mismatch between the refractive indices, and variations in the cover glass thickness. We have studied FCS near the surface of a cover glass by using rhodamine 123 as a fluorescent probe and have observed that the surface has a strong influence on the measurements. The temporal autocorrelation of FCS decays with two characteristic times when the confocal detection volume is positioned near the surface of the cover glass. As the position of the detection volume is moved away from the surface, the FCS autocorrelation becomes one-component decaying; the characteristic time of the decay is the same as the faster-decaying component in the FCS autocorrelation near the surface. This observation suggests that the faster component can be attributed to the free diffusion of the probe molecules in the solution, while the slow component has its origin from the interaction between the probe molecules and the surface. We have characterized the surface contribution to the FCS measurements near the surface by changing the position of the detection volume relative to the surface. The influence of the surface on the diffusion of the probe molecules was monitored by changing the chemical properties of the surface. The surface contribution to the temporal autocorrelation of the FCS strongly depends on the chemical nature of the surface. The hydrophobicity of the surface is a major factor determining the surface influence on the free diffusion of the probe molecules near the surface.

Examining Interaction Patterns in Online Discussion through Multiple Lenses

  • HAN, Seungyeon
    • Educational Technology International
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    • v.15 no.2
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    • pp.117-141
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    • 2014
  • This qualitative study investigated different interaction patterns in an online discussion. The data was collected from asynchronous discussion occurred in a graduate course. The data analysis methods include inductive analysis and mapping strategy. The results of the study suggest three layers of interaction: response sequences, interaction amongst participants, and concept map of messages. The visualization of response sequences enabled the researcher to discover complex and dynamic interaction patterns amongst participants. The many-to-many communication feature of online discussion does not always enable direct one-on-one interaction between two participants. Rather, one message contributed to multiple threads in the stream of conversation. In terms of interaction amongst participants, the interaction amongst participants, as indicated in the data, the messages also bind each participant and consequently a group(s) of participants together. It appears that the contribution of one message may not only enable a response to one participant, but also connect many participants to each other. The concept map of messages proposes that response sequences and interaction amongst participants can also be viewed between concepts within messages in the discussion. On the surface, the messages posted by individuals are linked by the system in a linear fashion as they are posted. However, the interaction extends to collaborative conversation amongst participants. Ultimately, a conceptual network of interrelated ideas including multiple perspectives is built in asynchronous discussion.

Fluid-structure interaction analysis of micromechanical resonance sensor (마이크로기계 공진 센서의 유체-구조물 상호 작용 해석)

  • Kang, In-Goo;Shin, Yoon-Hyuk;Yim, Hong-Jae;Lim, Si-Hyung
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2009.10a
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    • pp.309-313
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    • 2009
  • A micromechanical resonance sensor detects the resonance frequency shift due to mass or adsorption induced surface stress change during molecular adsorption or interaction on its surface. The resonance sensor is surrounded by gas or liquid solution during operation. To study the resonance shift phenomena depending on its surrounding environment, fluid-structure interaction of the resonance sensor has been analyzed for the different fluid environment and boundary conditions using finite element analysis.

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Evaluation on Interaction Surface of Plastic Resistance for Exposed-type Steel Column Bases under Biaxial Bending

  • Choi Jae-hyouk;Ohi Kenichi
    • Journal of Mechanical Science and Technology
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    • v.19 no.3
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    • pp.826-835
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    • 2005
  • Exposed-type steel column bases are used widely in low-rise building construction. Numerous researchers have examined methods to identify their stiffness and strength, but those studies have heretofore been restricted to in-plane behaviors. This paper presents an experimental investigation of inelastic behaviors of square hollow section (SHS) steel column bases under biaxial bending. Two types of failure modes are considered : anchor bolt yielding and base plate yielding. Different pinching effects and interaction surfaces for biaxial bending are observed for these two modes during bi-directional quasi-static cyclic loading tests. Differences are elucidated using limit analyses based on a simple analytical model.

Fluid-structure interaction analysis of sloshing in an annular - sectored water pool subject to surge motion

  • Eswaran, M.;Goyal, P.;Reddy, G.R.;Singh, R.K.;Vaze, K.K.
    • Ocean Systems Engineering
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    • v.3 no.3
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    • pp.181-201
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    • 2013
  • The main objective of this work is to investigate the sloshing behavior in a baffled and unbaffled three dimensional annular-sectored water pool (i.e., tank) which is located at dome region of the primary containment. Initially two case studies were performed for validation. In these case studies, the theoretical and experimental results were compared with numerical results and good agreement was found. After the validation of present numerical procedure, an annular-sectored water pool has been taken for numerical investigation. One sector is taken for analysis from the eight sectored water pool. The free surface is captured by Volume of Fluid (VOF) technique and the fluid portion is solved by finite volume method while the structure portions are solved by finite element approach. Baffled and un-baffled cases were compared to show the reduction in wave height under excitation. The complex mechanical interaction between the fluid and pool wall deformation is simulated using a partitioned strong fluid-structure coupling.

A Simulation of Flame-Vortex Interaction considering the Alteration of Vortex by Flame (와동의 변화를 고려한 화염-와동 상호 작용 모사)

  • Kang, Ji-Hoon;Kwon, Se-Jin
    • 한국연소학회:학술대회논문집
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    • 2000.05a
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    • pp.189-196
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    • 2000
  • A numerical simulation was conducted to analyze the interaction of flame and vortices. The characteristic scales of flame and vortices were limited in the thin laminar flamelet regime. Within this regime, flame is assumed as discontinuity surface and its motion in flow field was described by G-equation instead of full governing equations. Additional approximations include distribution of line volume sources on flame surface to simulate effect of volume expansion. Contrast to previous calculations, current study employed vortex transport equation to evaluate attenuation and smearing of vortices. Two extreme conditions of frozen vortex and frozen flame were considered to validate the current method. Comparison with direct numerical simulation resulted in satisfactory quantitative agreement with higher computational efficiency which warrants the usefulness of the present model in more complex situation.

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Thermodynamics of Partitioning of Substance P in Isotropic Acidic Bicelles

  • Baek, Seung Bin;Lee, Hyeong Ju;Lee, Hee Cheon;Kim, Chul
    • Bulletin of the Korean Chemical Society
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    • v.34 no.3
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    • pp.743-748
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    • 2013
  • The temperature dependence of the partition coefficients of a neuropeptide, substance P (SP), in isotropic acidic bicelles was investigated by using a pulsed field gradient nuclear magnetic resonance diffusion technique. The addition of negatively charged dimyristoylphosphatidylserine to the neutral bicelle changed the SP partitioning a little, which implies that the hydrophobic interaction between the hydrophobic residues of SP and the acyl chains of lipid molecules is the major interaction while the electrostatic interaction is minor in SP binding in a lipid membrane. From the temperature dependence of the partition coefficients, thermodynamic functions were calculated. The partitioning of SP into the acidic bicelles is enthalpy-driven, as it is for small unilamellar vesicles and dodecylphosphocholine micelles, while peptide partitioning into a large unilamellar vesicle is entropy-driven. This may mean that the size of lipid membranes is a more important factor for peptide binding than the surface curvature and surface charge density.

Simulation of Vehicle-Track-Bridge Dynamic Interaction by Nonlinear Hertzian Contact Spring and Displacement Constraint Equations (비선형 헤르쯔 접촉스프링과 변위제한조건식의 적용에 의한 차량-궤도-교량 동적상호작용 수치해석기법)

  • Chung Keun-Young;Lee Sung-Uk;Min Kyung-Ju
    • Proceedings of the KSR Conference
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    • 2005.11a
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    • pp.191-196
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    • 2005
  • In this study, to describe vehicle-track-bridge dynamic interaction phenomena with 1/4 vehicle model, nonlinear Hertzian contact spring and nonlinear contact damper are introduced. In this approach external loads acting on 1/4 vehicle model are self weight of vehicle and geometry information of running surface. The constraint equation on contact surface is implemented by Penalty method. Also, to improve the numerical stability and to maintain accuracy of solution, the artificial damper and the reaction from constraint violation are introduced. A nonlinear time integration method, in this study, Newmark method is adopted for both equations of vehicles and structure. And to reduce the error caused by inadequate time step size, adaptive time-stepping technique is partially introduced. As the nonlinear Hertzian contact spring has no resistance to tensile force, the bouncing phenomena of wheelset can be described. Thus, it is expected that more versatile dynamic interaction phenomena can be described by this approach and it can be applied to various railway dynamic problems.

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Thermodynamic interaction parameter between Zn and Cu, Ag, In, Bi, Pb, Sn in Dilute cadmium alloy by Touch Instant electro-motive force method (순간접전기전력법에 의한 용융 Cd중의 Zn과 Cu, Ag, In, Bi, Pb 및 Sn와의 상위작용 파라미터)

  • 김대룡;윤겸하
    • Journal of the Korean institute of surface engineering
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    • v.15 no.4
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    • pp.192-207
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    • 1982
  • A study has been made of the interaction parameters of Zn other elements in dilute solutesd solution of molten cadmium alloys over the temperature range of 450 to 570$^{\circ}C$. The experi-mental measurementss were made in a touch instant cell using a fusedd Licl-KCl electrolyte. The activity of zinc in binary and ternary solutions sexhibiteds a strong positive deviation from Raoult's law. The addition of silver, indium or lead increased the activity of zinc whereas addition of copper, bismuth or tin decreased the zinc activity slightly. The results for all the metallic solutions showed a linear dependence of reciprocal of ab-solute temperature over the experimental range. The interaction parameters obtained are as follows.

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A new way to design and construct a laminar box for studying structure-foundation-soil interaction

  • Qin, X.;Cheung, W.M.;Chouw, N.
    • Earthquakes and Structures
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    • v.17 no.5
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    • pp.521-532
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    • 2019
  • This paper describes the construction of a laminar box for simulating the earthquake response of soil and structures. The confinement of soil in the transverse direction does not rely on the laminar frame but is instead achieved by two acrylic glass walls. These walls allow the behaviour of soil during an earthquake to be directly observed in future study. The laminar box was used to study the response of soil with structure-footing-soil interaction (SFSI). A single degree-of-freedom (SDOF) structure and a rigid structure, both free standing on the soil, were utilised. The total mass and footing size of the SDOF and rigid structures were the same. The results show that SFSI considering the SDOF structure can affect the soil surface movements and acceleration of the soil at different depths. The acceleration developed at the footing of the SDOF structure is also different from the surface acceleration of free-field soil.