• Title/Summary/Keyword: Interaction Point

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Identification of Mutations in Protein Kinase CKIIβ Subunit That Affect Its Binding to Ribosomal Protein L41 and Homodimerization

  • Ahn, Bong-Hyun;Lee, Ji-Hoon;Bae, Young-Seuk
    • BMB Reports
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    • v.36 no.4
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    • pp.344-348
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    • 2003
  • Protein kinase CKII is composed of two catalytic ($\alpha$ or $\alpha$') subunits and two regulatory ($\beta$) subunits. The $CKII{\beta}$ subunit is thought to mediate the tetramer formation and interact with other target proteins. However, its physiological function remains obscure. In this study, point mutants of $CKII{\beta}$ that are defective for the L41 binding were isolated by using the reverse two-hybrid system. A sequence analysis of the point mutants revealed that Asp-26, Met-52, and Met-78 of $CKII{\beta}$ are critical for L41 binding; Asn-67 (and/or Lys-139) and Met-52 are important for $CKII{\beta}$ homodimerization. Two point mutants, R75 and R83, of $CKII{\beta}$ interacted with L5, topoisomerase $II{\beta}$, and CKBBP1/SAG, but not with the wild-type $CKII{\beta}$. This indicates that $CKII{\beta}$ homodimerization is not a prerequisite for its binding to target proteins. These $CKII{\beta}$ point mutants may be useful in exploring the biochemical physiological functions of $CKII{\beta}$.

Children's Clothing Preference on Animation Character Color -Focusing on Mickey and Mini Mouse- (애니메이션 캐릭터 색채에 대한 유아의 의복 선호도 -미키와 미니마우스를 중심으로-)

  • Sung, Nam-Suk;Choi, Su-Koung
    • Journal of the Korea Fashion and Costume Design Association
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    • v.13 no.2
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    • pp.63-71
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    • 2011
  • The purpose of this study was to analyze children's clothing preference on animated character color. The experimental materials developed for this study were a set of stimulus and response scales. The stimuli were 30 color pictures, in which gender (boy, girl), type of character (Mickey Mouse, Mini Mouse), hue of character (red, yellow, green, blue, purple), and tone of character (vivid, light, dark) were manipulated. The 5-point scale was used to evaluate children's clothing preferences. Data were obtained from 300 boys and 300 girls living in Seoul, Busan, Jinju, and Changwon in April 2010. For data analysis, ANOVA and Duncan-test were used by using SPSS program. Results of this study were as follows. Tone of character had an independent effect on children's clothing preference. Interaction effects of gender and hue of the character were found. Interaction effects of gender and tone of the character were found. Interaction effects of type and tone of the character were found.

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The Effect of the Types of Sex-appeal Fashion POP on Attitude toward Advertising: Focusing on the Moderating Role of Gender and Sensation Seeking

  • Park, Hyun Hee;Jeon, Jung Ok
    • International Journal of Human Ecology
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    • v.15 no.1
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    • pp.64-71
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    • 2014
  • This study empirically compares the influence of sex-appeal fashion Point-of-Purchase (POP) types on attitude towards advertising. It also highlights the moderating roles of gender and the level of sensation seeking. Three sex-appeal fashion POP types (nude/semi-nude/body conscious) were developed for this study and the interaction effect of the types of sex-appeal fashion POP advertising and gender was tested. In addition, interaction effect of the types of sex-appeal fashion POP advertising and the level of sensation seeking (high/low) was tested. The results found that gender influenced consumer attitudes toward sex-appeal fashion POP advertising type. Male consumers showed a more favorable attitude toward advertising for the nude type than others; however, female consumers showed a more favorable attitude toward advertising for semi-nude and body conscious type than the nude type. The results also found a significant two-way interaction effect of sexual POP advertising types and sensation seeking on attitude toward POP advertising. High sensation seekers preferred nude and semi-nude type POP advertising to body conscious POP advertising. It is useful to investigate how various marketing factors support attitude toward POP advertising in the context of sexual fashion POP advertising and which factors exert the strongest influence within different degrees of nudity.

A Study on the Virtual Mouse Interface System (가상 마우스 인터페이스 시스템에 관한 연구)

  • Lee, Ki-Young;Lim, Myung-Jae;Kim, Kyu-Ho;Lee, Min-Ki;Kim, Jeong-Lae
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.11 no.2
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    • pp.57-62
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    • 2011
  • Recently, various interaction was demanded from rapid development and use of portable device. So, operation of touch interface in tablet and smart phone needs many requirements and proactivity. In this paper, for variety of users and convenient interaction, It is aimed to realize virtual reality mouse of portable device. It reflected similar interaction of an existing mouse and it used infrared image without intensity of illumination. Also, It analyzed finger point information of middle and index finger of users and it designed virtual mouse without proactivity and constraint of space.

Unsteady Analysis of Impeller-Volute Interaction in Centrifugal Pump

  • Cheah, Kean Wee;Lee, Thong See;Winoto, Sonny H.
    • International Journal of Fluid Machinery and Systems
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    • v.4 no.3
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    • pp.349-359
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    • 2011
  • An unsteady numerical analysis has been carried out to study the strong impeller volute interaction of a centrifugal pump with six backward swept blades shrouded impeller. The numerical analysis is done by solving the three-dimensional Reynolds Averaged Navier-Stokes codes with standard k-${\varepsilon}$ two-equations turbulence model and wall regions are modeled with a scalable log-law wall function. The flow within the impeller passage is very smooth and following the curvature of the blade in stream-wise direction. However, the analysis shows that there is a recirculation zone near the leading edge even at design point. When the flow is discharged into volute casing circumferentially from the impeller outlet, the high velocity flow is severely distorted and formed a spiraling vortex flow within the volute casing. A spatial and temporal wake flow core development is captured dynamically and shows how the wake core diffuses. Near volute tongue region, the impeller/volute tongue strong interaction is observed based on the periodically fluctuating pressure at outlet. The results of existing analysis also proved that the pressure fluctuation periodically is due to the position of impeller blade relative to tongue.

Design and Development of Network Based Competition Learning Model (네트워크 기반의 다자간 상호 경쟁적 학습모형의 설계 및 플랫폼 구현)

  • Heo, Kyun
    • The KIPS Transactions:PartA
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    • v.10A no.6
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    • pp.709-714
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    • 2003
  • It is important that the interaction between learners and contents in Educational Contents. But, there is just simple interaction in traditional WBI or CAI. As it is necessary to study for interaction with learners. There is applied more multimedia elements for the fun of learners. But, it is also necessary to study for Network Educational Game Contents which can give virtual environment to learn easily and funny. In this study, Competition Learning Model is designed for network learning environment. We can look at the new view point of Educational Contents by implementation of Network Educational Game Contents and Competition Learning Model.

A Comparative Study on Models of Web-based Information Seeking Behavior (웹 정보탐색행위 모형의 비교 분석 연구)

  • 김성진
    • Journal of the Korean Society for information Management
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    • v.21 no.2
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    • pp.211-233
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    • 2004
  • The web is a new information environment, which has different characteristics from a traditional IR environment. Needed are more research from a new point of view as well as the adoption of a new research paradigm in order to understand a user-system interaction on the web. The purpose of this study is to review and analyze models of web-based information seeking behavior, which Wang, Hawk & Tenopir, Hsieh-Yee, Choo, Detlor & Turnbull, Chun & Cooper, Rieh, and Spink proposed. The comparative analysis indicates that web-based information seeking models are categorized into three area: interaction model, information seeking behavior model, and evaluation model, and that they are based on a multifaceted interaction and a nonlinear perspective.

Partitioned coupling strategies for fluid-structure interaction with large displacement: Explicit, implicit and semi-implicit schemes

  • He, Tao
    • Wind and Structures
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    • v.20 no.3
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    • pp.423-448
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    • 2015
  • In this paper the unsteady fluid-structure interaction (FSI) problems with large structural displacement are solved by partitioned solution approaches in the arbitrary Lagrangian-Eulerian finite element framework. The incompressible Navier-Stokes equations are solved by the characteristic-based split (CBS) scheme. Both a rigid body and a geometrically nonlinear solid are considered as the structural models. The latter is solved by Newton-Raphson procedure. The equation governing the structural motion is advanced by Newmark-${\beta}$ method in time. The dynamic mesh is updated by using moving submesh approach that cooperates with the ortho-semi-torsional spring analogy method. A mass source term (MST) is introduced into the CBS scheme to satisfy geometric conservation law. Three partitioned coupling strategies are developed to take FSI into account, involving the explicit, implicit and semi-implicit schemes. The semi-implicit scheme is a mixture of the explicit and implicit coupling schemes due to the fluid projection splitting. In this scheme MST is renewed for interfacial elements. Fixed-point algorithm with Aitken's ${\Delta}^2$ method is carried out to couple different solvers within the implicit and semi-implicit schemes. Flow-induced vibrations of a bridge deck and a flexible cantilever behind an obstacle are analyzed to test the performance of the proposed methods. The overall numerical results agree well with the existing data, demonstrating the validity and applicability of the present approaches.

Differences in Perceived Risk and Product Attitudes : Focus on Korea and Thailand Consumers

  • Kim, Moon-Jung;Kim, Eun-Hee
    • The Journal of Industrial Distribution & Business
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    • v.8 no.6
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    • pp.41-49
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    • 2017
  • Purpose - The purpose of this research is to examine the differences and interaction effects of perceived risk and product attitudes between Korea and Thailand consumers in accordance with price discount and product types. Research design, data, and methodology - A questionnaire survey was conducted in Korea and Thailand. There were a total of 327questionnaires received, and 322 of them were valid. Respondents consisted of 163 Koreans and 159 Thai consumers. Each question is measured in a Likert-type five-point scale. To verify the difference and interaction effects of perceived risk and product attitudes, ANOVA analysis was carried out. Results - This research found that the perceived risk of Thailand consumers in accordance with price discount and product types is confirmed to be larger than Korean consumers. The difference of product attitudes of Thailand consumers is higher than those of Korean consumers. Thus, product attitudes in accordance with country type and price discount types are verified with the interaction effect. The difference of product attitudes in accordance with price discount type and product types are not founded. The main effect was not verified. Conclusions - The finding of this study can be used as useful information to Korean and Thai retailers looking to enter the global market.

On the Manoeuvring Motion Considering the Interaction Forces in Confined Waters

  • Lee, Chun-Ki;Kang, Il-Kwon
    • Journal of Navigation and Port Research
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    • v.27 no.6
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    • pp.639-643
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    • 2003
  • The emphasis is put on the detailed knowledge on manoeuvring characteristic for the safe navigation while avoiding terrible collision between ships and on the guideline to the design and operation of the ship-waterway system The numerical simulation of manoeuvring motion was carried out parametrically for different ship types, ship-velocity ratios, separation and stagger between ships. As for the calculation parameters, the ratios of velocity difference (hereafter, $U_2$/$U_1$ ) between two ships were considered as 0.6, 1.2, 1.5. From the inspection of this investigation, it indicates the following result. Considering the interaction force only as parameter, the lateral distance between ships is necessarily required for the ship-velocity ratio of 1.2, compared to the cases of 0.6 and 1.5 regardless of the ship types. Furthermore, regardless of the ship-velocity ratio, an overtaking and overtaken vessel can be manoeuvred safely without deviating from the original course under the following conditions: the lateral distance between two vessels is approximately kept at 0.5 times of ship-length and 5 through 10. degrees of range in maximum rudder angle. The manoeuvring characteristic based on this investigation will be very useful for keeping the safety of navigation from the practical point of ships design and traffic control in restricted waterways.