• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4331-4338
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• 2014

$N^1$-(2, 5-dimethoxyphenyl)-$N^8$-hydroxyoctanediamide (N25) is a novel SAHA cap derivative of HDACi, with a patent (No. CN 103159646). This invention is a hydroxamic acid compound with a structural formula of $RNHCO(CH_2)6CONHOH$ (wherein R=2, 5dimethoxyaniline), a pharmaceutically acceptable salt which is soluble. In the present study, we investigated the effects of N25 with regard to drug distribution and molecular docking, and anti-proliferation, apoptosis, cell cycling, and $LD_{50}$. First, we designed a molecular approach for modeling selected SAHA derivatives based on available structural information regarding human HDAC8 in complex with SAHA (PDB code 1T69). N25 was found to be stabilized by direct interaction with the HDAC8. Anti-proliferative activity was observed in human glioma U251, U87, T98G cells and human lung cancer H460, A549, H1299 cells at moderate concentrations ($0.5-30{\mu}M$). Compared with SAHA, N25 displayed an increased antitumor activity in U251 and H460 cells. We further analyzed cell death mechanisms activated by N25 in U251 and H460 cells. N25 significantly increased acetylation of Histone 3 and inhibited HDAC4. On RT-PCR analysis, N25 increased the mRNA levels of p21, however, decreased the levels of p53. These resulted in promotion of apoptosis, inducing G0/G1 arrest in U251 cells and G2/M arrest in H460 cells in a time-dependent and dose-dependent manner. In addition, N25 was able to distribute to brain tissue through the blood-brain barrier of mice ($LD_{50}$: 240.840mg/kg). In conclusion, our findings demonstrate that N25 will provide an invaluable tool to investigate the molecular mechanism with potential chemotherapeutic value in several malignancies, especially human glioma.

• The Korean Journal of Malacology
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• v.27 no.3
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• pp.247-252
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• 2011

The effect of ${\gamma}$-ray irradiation on the color of nucleus and cultured pearls was investigated. After ($^{60}Co$) ${\gamma}$-ray irradiation on the pearl nucleus, its color was altered from brownish to blackish brown or gray depending on irradiation dose. It was clearly found that the all samples are composed of aragonite. ESR spectra were based on the measurement of the paramagnetic species $CO_2$-radicals by the interaction with the ${\gamma}$-ray irradiation in $Mn^{2+}$ containing $CaCO_3$. In this study, we investigated optimal detection method of ${\gamma}$-ray irradiation to establish destructive or non-destructive test for pearl nucleus.

• Journal of Digital Convergence
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• v.11 no.4
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• pp.409-414
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• 2013

The purpose of this study is to verify the effects of partial-cyber and full-cyber lectures and explore directions for improvement. This study compared the mean scores of course evaluation for traditional face-to-face lectures, partial-cyber lectures of blended instruction, and full-cyber lectures. Also, this study interviewed instructors of full-cyber lectures to investigate the ways to enhance the lecture quality. The findings suggest that the course evaluation scores for full-cyber university were consistently lower than those for other types of lectures for four semesters between the years of 2011 and 2012. Results also showed that mean scores of partial-cyber lectures were the same as those of face-to-face lectures. After all, class satisfaction in full-cyber courses that learning occurs in cyber space was the lowest. Instructors who taught full-cyber lectures proposed that enrollment should not be within 60 students and professional assistance should be provided for lectures exceeding 60 students. Finally, they suggested content updates through a collaborative system with professionals, instructors' efforts to enhance interaction in both online and offline contexts, and learning quantity rationalization.

• Asian Journal of Turfgrass Science
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• v.6 no.2
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• pp.73-81
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• 1992

This study was designed to estimate on the interaction of the first productivity, light condition and to examine the ecophysiological 'characteristics of Zoysia japonica. Artificial community of Zoysia japonica was analyzed effect of matter production and growth construction under various shading condition. The results summarized this experiment were as follows ; 1. The relative growth rate(RGR) in all experimental plots was high during the growth stage of 20 days after transplanting and then tend to decrease. RGR value in severe shading or short day condition was remarkably decreased from the early growth stage. 2. The net assimilation rate(NAR) was high during the early growth stage after transplanting. and then decreased the growth proceeded. NAR value in the severe shading or short day condition plots was low. 3. The increasing rate of leaf ared ratio(LAR) was high during the early growth stage after transplanting in the control plot and 30% shading plot, but it appeared to be high 40 days after transplanting in the 50% and 70% shading plot. LAR was appeared to be negative correlation against RGR and NAR. 4. The crop growth rate(CGR) in each experimental plot was increased until 50 days after transplanting, and then decreased. After that time, the maximum CGR value appeared in the control plot(1.56g /g, 10days), 60days after transplanting. CGR had positive correlation with LAI. The optimum LAI in the control, 30%, 50%, and 70% shading plots was appeared as 2.10, 1.75, 1.25, and 0.90.

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.4
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• pp.597-606
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• 2008

Resorption of alveolar bone in periodontitis is due to excessive differentiation and activation of osteoclasts. Bacterial antigens causing periodontitis activates CD4 T cells, which leads to expressing RANK ligand (RANKL) on CD4 T cells. RANKL binds RANK on preosteoclasts or osteoclasts, and enhances the differentiation preosteoclasts into osteoclasts and the activation of mature osteoclasts. CD137, one of TNF receptor (TNFR) family, expressed on activated T cells binds with CD137 ligand (CD137L) on antigen presenting cells. Cross-linking of CD137 by CD137L acts as T cell co-stimulatory signals and, therefore, enhances the activation of T cell. In this study, I elucidated the biological responses of CD137L on (pre)osteoclasts and RANKL on T cells in the context of in vivo interaction between T cells and osteoclasts. RAW264.7, murine monocytic cells, constitutively express CD137L. Ligation of CD137L with anti-CD137L mAb inhibited RANKL-induced osteoclast formation in a dosedependent manner. Bone marrow cells are expressed CD137L by the treatment with M-CSF. Cross-linking of CD137L abolished M-CSF/ RANKL-evoked the formation of multi-nucleated osteoclasts. Both mouse CD4 and CD8 T cells are expressed RANKL following their activation. Ligation of RANKL with OPG, the decoy receptor for RANKL, inhibited both CD4 and CD8 T cell proliferation. These effects were attributed to RANKL-induced apoptosis. These data indicate that CD137L and RANKL on osteoclasts and T cells, respectively provide them with inhibitory signal.

• Korean Chemical Engineering Research
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• v.46 no.2
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• pp.356-368
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• 2008

The effects of cosurfactant on silica nanoparticles were investigated in systems containing surfactant, oil and aqueous ammonia solution where nanoparticles were prepared using a single phase water-in-oil (W/O) microemulsion. For the same oil phase, a single phase region was dependent on the interaction between surfactant and oil. For the cyclohexane system, NP-5 surfactant showed a wider single phase region than NP-4. The addition of n-propanol as a cosurfactant resulted in an increase or a decrease of a single phase W/O microemulsion region depending on the continuous oil phase. For both cyclohexane and isooctane systems, the addition of n-propanol resulted in a decrease in the single phase region. On the other hand, for n-heptane system, the addition of n-propanol expanded a single phase W/O microemulsion region. Silica nanoparticles prepared within a single phase region showed that relatively large number of particles of irregular shape were obtained with the addition of n-propanol to NP surfactant system. The addition of n-propanol to LA-5 surfactant and n-heptane system produced a decrease in average particle size and an increase in the number of particles formed due to a decrease in the intermicellar exchange rate among microemulsion droplets.

• Journal of Korean Academy of Nursing
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• v.18 no.1
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• pp.5-18
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• 1988

Herzberg and his associates concluded from their findings that job satisfaction consisted on two independent dimension ; the first dimension was related to job satisfaction, the second to job dissatisfaction. According to the Herzberg theory, the satisfiers are related to the nature of the work itself and the rewards that flow directly from the performance of that work. The dissatisfaction factors are associated with the individual's relation to the context or environment in which he works. The purpose of this study was to investigate selected factors which result in job satisfaction / dissatisfaction of nurses employed in general hospital. In a study of this nature, it is important that the population be as homogenous as possible in order to reduce the effects of the different environments and backgrounds on job satisfaction. A job satisfaction questionnaire developed by Slabitt et als. was used for this study. It contains 45 statements and utilizes a Likert type scale of 5. Participants were asked to select response which were congruent with their perceptions of the item. It was decided to conduct the study in one general hospital in Seoul. A sample of 505 clinical nurses were selected to participate in this study. The results of this study were as follows ; 1. The overall mean score for the 45 five-point scales of job satisfaction items was 2.945, showing that the subjects of this study were neither satisfied nor dissatisfied. 2. To identify the specific job related factors that result in Job satisfaction / dissatisfaction, the 45 items of job satisfaction were divided into 6 areas and the mean scores and % of agreement were compared. The specific job related factor that resulted in job satisfaction was the job status and those that resulted in job dissatisfaction were salaries, task requirements and organization requirements. But the areas of autonomy and interaction did not belong to either side of the specific job related factors. 3. To identify the relationship between the job satisfaction and the subject's general characteristic, data was analyzed using the t-test and the Pearson correlation coefficient. It was found that the relationship between the job satisfaction and the request for rotation and intention to remain on the job were statistically significant at .05 level, but the relationship between the job satisfaction and age, work experience, and educational background were nor statistically significant at the .05 level.

• Korean journal of communication and information
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• v.32
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• pp.369-405
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• 2006

The purpose of this paper is to examine the usefulness of the concept of political efficacy and political trust as a way of explaining the development of political participation in the young people under the new age of the Internet. From the survey in which 221 university students participated, there were several findings: first, overall political efficacy has a predominant effect on political participation in both offline and online; second, political trust has little impact on the political participation of the youth; third, there showed up two interaction effects between political efficacy and political trust for the online conventional and unconventional participation in politics. It is found that in high efficacy group people with low trust are most likely to participate, while in medium efficacy group people with high trust are most likely to participate. In low efficacy group, however, political trust didn't make any difference in political participation. There are more factors that may predict political participation, such as political interest, political knowledge, political ideology, and demographic variables. For future studies, it is necessary to examine the effect of political efficacy and political trust in combination with these other variables to explain political participation by offline and online.

• Journal of the Korea Concrete Institute
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• v.15 no.4
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• pp.513-521
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• 2003

Existing tests results have shown that confining the concrete compression region with closed stirrups improves the ductility and load-carrying capacity of beams. However, only few researchers have attempted to utilize the beneficial effects of the presence of these stirrups in design. This paper presents the result of experimental studies on the load-deflection behavior and the strengthening effect of laterally confined structural high-strength concrete beam members in which confinement stirrups have been introduced into the compression regions. Fifteen tests were conducted on full-scale beam specimens having concrete compressive strength of 41 MPa and 61 MPa. Different spacing of stirrups(0.25∼1.0d) and amount of tension steel($0.55{\sim}0.7{\rho}_b$) as major variables were investigated. And also, this study present an appropriate shear equation for decision of ultimate failure modes of high-strength concrete beams according to stirrup spacing. The equation is based on interaction between shear strength and displacement ductility. Prediction of failure mode from presented method and comparison with test results are also presenteded

• Journal of Korean Society of Forest Science
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• v.109 no.1
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• pp.31-40
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• 2020

This study aimed to investigate the effects of climate change on the survival and growth performance of Pinus densiflora and Larix kaempferi seedlings using open-field experimental warming and precipitation manipulation. We measured the survival rate, root-collar diameter, and height, and then calculated the seedling quality index (SQI) of 2-year-old seedlings under 6 treatments [2 temperatures (TC: Control; TW: Warming) × 3 precipitation manipulations (PC: Control; PD: Decreased; PI: Increased)] and performed a two-way ANOVA to test for differences.The air temperature of the warming plots was 3℃ higher than that of the control plots, while the precipitation manipulation plots received ±40% of the precipitation received by the control plots. Temperature and precipitation treatments did not significantly affect the survival rate of P. densiflora; however, the SQI of P. densiflora decreased with increasing precipitation. In contrast, the mortality rate of L. kaempferi increased with increasing temperature and decreasing precipitation. Furthermore, in L. kaempferi, TC × PI treatment resulted in the lowest SQI with a significant interaction effect observed between the two factors. In summary, low seedling production and quality should be expected in P. densiflora as precipitation increases and in L. kaempferi as temperature increases or precipitation decreases. These results indicate species-specific sensitivities to climate change of two plant species at the nursery stage. With the occurrence of global warming, the frequencies of drought and heavy rainfall events are increased, and this could affect the survival and seedling quality of tree species. Therefore, it is necessary to improve nursery techniques by establishing new adaptation strategies based on species-specific growth performance responses.