• Bulletin of the Korean Chemical Society
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• v.24 no.1
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• pp.70-74
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• 2003

The heterogeneous bioluminescence immunoassay for digoxin was developed using photoprotein, native aequorin as a label and the site-directed immobilization technique based on avidin/biotin interaction. Aequorin is a bioluminescence protein, originally isolated from the jellyfish Aequoria Victoria and an attractive label in analytical applications because of sensitive detection due to virtually no background bioluminescent signal. Digoxin is a cardioactive drug, and its therapeutic level in serum is at low concentration with very narrow therapeutic index. The aequorin-digoxigenin conjugates were synthesized by the N-hydroxysuccinimide ester method and characterized in terms of bioluminescent residual activity. The resulting dose-response curve shows that the detection limit is $1.0\;{\times}\;10^{-10}\;M$ and a dynamic range is three orders of magnitude, which was obtained by $1.0\;{times}\;10^{-10}\;M$ conjugate and 0.9 μg/mL anti-digoxin antibody. Three structurally similar molecules to digoxin were examined for their cross-reactivity. None of these three compounds showed any crossreactivity with digoxin antibody employed in this study. Standard amounts of digoxin corresponding to the therapeutic range were spiked into the each serum solution. Study of the serum matrix effect indicated that correlation coefficient shows good agreement between luminescence light intensity between in buffer and in serum.

• Bulletin of the Korean Chemical Society
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• v.24 no.12
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• pp.1781-1784
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• 2003

Determination of Co(II) ion as a 4-(2-thiazolylazo)resorcinol(TAR) or 5-methyl-4-(2-thiazolylazo)resorcinol(5MTAR) chelate was accomplished by reversed-phase capillary high-performance liquid chromatography (RP-Capillary-HPLC) using a Vydac $C_4$ column and MeCN-water mixture as mobile phase. The effect of change in pH and MeCN percentage of the mobile phase on the retention factor, k and peak intensity were evaluated. It was found that 30% MeCN (v/v) of pH 5.60 or 7.20 was adequate as mobile phase when TAR or 5MTAR is used. Detection limit (D.L., S/N=3) in each case was $2.0\;{\times}\;10^{-7}$M (11.8 ppb) and $3.0\;{\times}\;10^{-7}$ M (17.7 ppb). The Co(II) ion in mineral and waste water was determined with the optimum column and mobile phase.

• Bulletin of the Korean Chemical Society
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• v.30 no.5
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• pp.1026-1030
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• 2009

A novel Schiff base ligand (N, O system) 5-methoxy-2-phenyliminomethylphenol ($5-CH_3O-PMP$) was synthesized. Using the synthesized ligand as a fluorescent reagent, a fluorometric method was developed for the quantitative analysis of Al(III) ion. The quantitative analysis of Al(III) ion was performed by making the complex compound between Al(III) ion and $5-CH_3O-PMP$ in ethanol-water solution (85/15, v/v, pH 6.2). The excitation wavelength (${\lambda}em$) of the complex compound was 397 nm while the emmision wavelength (${\lambda}em$) was 498 nm. The quantitative analysis of Al(III) ion was carried out by estimating the fluorescence intensity. The various calibration curves were used for the quantitative analysis in the range of 0.27$\sim$27 ng/mL Al(III) ion concentrations. The detection limit was 0.027 ng/mL. Using the fluorometric method developed in this study, satisfying results were obtained from various samples such as tap water, hot spring water, river water, sea water and waste water, which contained considerable amounts of interfering ions.

• Journal of the Korea Institute of Military Science and Technology
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• v.23 no.3
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• pp.204-212
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• 2020

For general target tracking works by receiving a set of measurements from sensor. However, if the SNR(Signal to Noise Ratio) is low due to small RCS(Radar Cross Section), caused by remote small targets, the target's information can be lost during signal processing. TBD(Track Before Detect) is an algorithm that performs target tracking without threshold for detection. That is, all sensor data is sent to the tracking system, which prevents the loss of the target's information by thresholding the signal intensity. On the other hand, using all sensor data inevitably leads to computational problems that can severely limit the application. In this paper, we propose an iterative Joint Integrated Probabilistic Data Association as a practical target tracking technique suitable for a low SNR multi-target environment with real time operation capability, and verify its performance through simulation studies.

• Bulletin of the Korean Chemical Society
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• v.30 no.12
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• pp.3034-3038
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• 2009

A simple, rapid, practical and sensitive spectofluorimetric method was developed for the determination of trace amount of heparin (Hep). Under the Optimum conditions, we studied the interaction between NFLX-Ce$^{3+}$-Hep complex by using absorption and fluorescence spectra. It was observed that Hep remarkably enhance the fluorescence intensity of the NFLX-Ce$^{3+}$ complex at ${\lambda}$= 356 nm in the buffer solution of pH = 7.60 and the enhancement effect is shown to relate with the concentration of Hep. The linear range and detection limit for the determination of Hep was obtained. By the Rosenthal graphic method, the association constant (K) and binding numbers (N) of Hep with probe were investigated. This method is relatively free of interference from coexisting substances and successfully applied for the determination of heparin in heparin sodium injection samples. A suitable mechanism of fluorescence enhancement between NFLX-Ce$^{3+}$ and the NFLX-Ce$^{3+}$-Hep systems were proposed and discussed.

• Steel and Composite Structures
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• v.1 no.4
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• pp.411-426
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• 2001

This paper treats the failure analysis of prestressing steel wires with different kinds of localised damage in the form of a surface defect (crack or notch) or as a mechanical action (transverse loads). From the microscopical point of view, the micromechanisms of fracture are shear dimples (associated with localised plasticity) in the case of the transverse loads and cleavage-like (related to a weakest-link fracture micromechanism) in the case of cracked wires. In the notched geometries the microscopic modes of fracture range from the ductile micro-void coalescence to the brittle cleavage, depending on the stress triaxiality in the vicinity of the notch tip. From the macroscopical point of view, fracture criteria are proposed as design criteria in damage tolerance analyses. The transverse load situation is solved by using an upper bound theorem of limit analysis in plasticity. The case of the cracked wire may be treated using fracture criteria in the framework of linear elastic fracture mechanics on the basis of a previous finite element computation of the stress intensity factor in the cracked cylinder. Notched geometries require the use of elastic-plastic fracture mechanics and numerical analysis of the stress-strain state at the failure situation. A fracture criterion is formulated on the basis of the critical value of the effective or equivalent stress in the Von Mises sense.

• Structural Engineering and Mechanics
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• v.6 no.1
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• pp.77-93
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• 1998

Some design formulas and design procedures for single and multiple tuned liquid column dampers (TLCDs) are proposed in this study. Previous studies show that if the properties of the TLCD system are properly selected then the TLCD could be as effective as the traditional tuned mass dampers. In addition, the TLCD system offers advantages such as flexibility in terms of installation, little maintenance required, and potentials for multiple usage, etc., which are incomparable by other mechanical types of dampers. In this paper, a set of optimal properties such as length and head loss of a TLCD system are derived under the assumption that the building vibrates in a dominate mode and is subjected to Gaussian white noise excitation. A design procedure for a single TLCD system will be illustrated and discussed. Due to the nonlinearity in the damping term, the TLCD system is sensitive to the loading intensity. This loading sensitivity could limit the application range of the TLCD system. It will be shown in this paper that such a nonlinear effect can be reduced by using multiple TLCDs. As a demonstrative example, the control effects on a flexible building modeled as a single degree-of-freedom system subjected to white noise excitation will be analyzed and discussed using single or multiple TLCDs.

• Wind and Structures
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• v.18 no.1
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• pp.1-20
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• 2014

Modern design of long suspension bridges must satisfy at the same time spanning very long distances and limiting their response against several external loads, even if of high intensity. Structural Control, with the solutions it provides, can offer a reliable contribution to limit internal forces and deformations in structural elements when extreme events occur. This positive aspect is very interesting when the dimensions of the structure are large. Herein, an updated numerical model of an existing suspension bridge is developed in a commercial finite element work frame, starting from original data. This model is used to reevaluate an optimization procedure for a passive control strategy, already proven effective with a simplified model of the buffeting wind forces. Such optimization procedure, previously implemented with a quasi-steady model of the buffeting excitation, is here reevaluated adopting a more refined version of the wind-structure interaction forces in which wind actions are applied on the towers and the cables considering drag forces only. For the deck a more refined formulation, based on the use of indicial functions, is adopted to reflect coupling with the bridge orientation and motion. It is shown that there is no variation of the previously identified optimal passive configuration.

• Bulletin of the Society of Naval Architects of Korea
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• v.12 no.2
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• pp.43-58
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• 1975

There are many reports on fatigue crack of metallic materials but most of them relate crack propagation rate to stress intensity factor. The problem of crack propagation is not yet clarified, especially the bridge between micro and macro phenomena In this experiment rotating bending fatigue tests have been carried out with smoothed specimen of rolled steel plates including 0.2% carbon under application of three stress conditions to investigate the slip band and the crack propagation behaviour. The results obtained are as follows; 1) The length of cracks which have grown at initial crack tips can be expressed as follows; $l=Ae^{BNr}$(A,B: constant, $N_r$: cycle ratio) $\frac{dl}{dN}=\frac{AB}{N_f}{\cdot}e^{BNr}$($N_f$:fatigue life) 2) The ratio of slipped grain number to total grain number is $S_f=7{\sigma}-5.6$-5.6{\sigma}_c$($\sigma$: stress amplitude) (${\sigma}_c$: fatigue limit) 3) When the fatigue process transfers from Stage I to Stage II, the crack which propagates into specimen changes its direction from that of the maximum shear stress to the direction of perpendicular to principal stress and this is same in the circumferential direction of specimen. the crack propagation behaviors of both sides of a crack are different each other when they approach to the grain boundary.

• Bulletin of the Korean Chemical Society
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• v.30 no.1
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• pp.102-106
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• 2009

A rapid and sensitive chemiluminescence (CL) method using flow-injection (FI) has been developed for the determination of mandelic acid which is based on the enhancement of mandelic acid to the CL intensity of ${Ru(bipy)_{3}}^{2+}$-Ce(IV) system. The enhancement effect was dependent on the concentration of mandelic acid, based on which, CL system was established for the determination of mandelic acid. The concentrations of ${Ru(bipy)_{3}}^{2+}$+, Ce(IV), and $H_2SO_4$ were optimized. Under the optimum experimental conditions, the linear range and detection limit are 1.46-342.0 ${\mu}g/ml$ and 0.072 ${\mu}g/ml$, respectively. The correlation coefficient (R) was 0.99732. The utility of this method was demonstrated by determining mandelic acid in capsules and human urine sample.