• Animal cells and systems
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• 제8권4호
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• pp.295-300
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• 2004

In the breeding colony of black-tailed gull, as nests of conspecific neighbors are very closely located, chicks are permanently exposed by sound and visual stimuli produced by adult conspecifics approaching their nests. The chicks, therefore, may need to learn ways to appropriately respond to their parents approach. In this study we experimentally manipulated sensory stimulation that is potentially provided by the parents to the offspring. Chicks incubated in the laboratory were exposed to a mew call of the conspecific adult. Then they were tested in three situations differing in sensory stimulation: 1) visual stimulation only, 2) auditory stimulation only, and 3) Simultaneous visual and auditory stimulations. We observed occurrence of different response of the chicks, which were categorized into three behaviors (begging call response, chirirah call and pecking behavior). We also investigated intensity of the chicks call in response to the different stimulations and the degree of response with age. The chicks exposed to only auditory stimulation made significantly more chirirah calls. The intensities (dB) of the mew call and chicks chirirah call were directly correlated. On the other hand, when chicks just saw the stuffed adult gull, they responded significantly more with a begging call and pecking behavior. In the situation of costimulation, the chicks responded with a begging call and pecking, but less frequently than visual stimulation only. The results suggest that young black-tailed gulls use call repertories to properly respond to parents behavior. Such results suggest an evolutionary process for uncreasing their survival rate in a group breeding site.

• 보존과학연구
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• 통권17호
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• pp.123-160
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• 1996

There have been many studies and experiments regarding exhibition lighting. Many experiments on photochemical damaging effect and visibility resulted in a practice limited to assigning light levels and adjusting annual exposure time. The three damaging factors to the artifacts are intrinsicsusceptibility to absorb radiant energy, spectral distribution of light source and intensity of illumination and time of exposure. Dividing all the artfacts into three categories to suggest a recommended illuminance level causes some problems. Blue wool, for example, used as the reference material for susceptibility, is not a standard material representing museum artifacts. In the most light sensitive category, ISO class I or anything below have been excluded. The exposure time of one soure can be three times more than another sourece. The spectral distribution of the light source and the relative spectral responsibility of the artifact are not considered in the practice. So in case of very light sensitive material, the recommended illuminance is only the referring value and it is indispensable to check the characteristic of susceptibility of each artifacts. Daylighting is prevailing method to solve the psychological need of the visitors. However, it sould transparent, and should not diffused, and the green-house effect must be considered. llluminance uniformity should based on the maximum illuminance to handle the limitation of exposure for the conservation of a large sensitive object such as a painting. Damage index is not absolute reference for selecting the lighting source because it is experimented from the paper of low grade then calculated. Visibility should be increased by reducing the visual noiseand by planning of appropriate luminance contrast. This paper reviews the problems with the previous studies and experiment sand the current exhibition lighting design practice. The plan for museum showcase lighting is to check the susceptibility and to raise the visibility simultaneously.

• 통합자연과학논문집
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• 제1권2호
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• pp.89-114
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• 2008

Interrelationship between heavy rainfalls and related with low-level jets(LLJ) is analyzed by using fifty cases of heavy rainfall events occurred over the Korean peninsula from 1992 to 2001. Those cases are classified with four synoptical features. There are 32% chances that the low pressure exist in heavy rainfall over than 150 mm per day case by case. Secondly Changma front and front zone account for 28% of all cases. The ratio of marine tropical boundary type and trough type record 22% and 18% respectively. The moist and warm south-westerly winds associated with low-level jets have been induced convective instability and baroclinic instability. Therefore, heavy rainfall due to the approach of a low pressure occurred at September and before Changma. During the period of Changma, this type has been happened heavy rainfall when low pressure and stationary front has vibrated south and north. Changma type has longer the duration time of precipitation than other types. Third type, located with marine Tropical boundary, have mainly rained in August and September. The last trough case locally downpoured in short time with developing cell. The occurrence low-level jets related to heavy rainfall has increased over 12.5 m/s wind speed. The result is that 43 heavy rainfalls out of 50 cases reach peak at the time of maximum precipitation intensity. Also, the variation of wet number and K-index corresponded with the variation of wind speed. It is found that the number of frequency of low-level jets with southwestward direction has been increased and these jets are mainly passed from the southwest toward to the northeast of the Korean peninsula in that time.

• 대한토목학회논문집
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• 제34권6호
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• pp.1919-1924
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• 2014

최근 기후변화로 인해 극한강우사상의 빈도 및 강도가 증가함에 따라 과거 기준에 의해 설계 시공되어 현재 운영 중인 댐의 수문학적 안전성에 의문이 제기되고 있다. 이에 따라 댐을 증고하거나 여수로를 확장/신설하는 방안이 추진되고 있는데, 이러한 대안을 선정할 때 월류 가능성에 매몰된 나머지 하류 홍수피해를 간과하는 경향이 있다. 이에 본 연구는 회복탄력성의 개념을 도입해 종합적인 홍수조절기능의 관점에서 댐의 안전성 강화 대안을 평가할 수 있는 프레임워크를 제시하였다. 제시된 프레임워크를 활용해 기 수행된 사례에 적용한 결과 각 대안에 따른 홍수유출특성이 선정과정에서 중요한 요소로 평가되어야 함을 확인할 수 있었다.

• 콘크리트학회논문집
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• 제27권2호
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• pp.147-155
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• 2015

본 연구에서는 기존 강도증진형 내진보강법의 단점을 보완 개선할 수 있는 새로운 개념의 내진보강법인 SRCF (Steel Reinforced Concrete Frame) 외부접합형 내진보강법을 제안하였다. SRCF 보강법은 거주자가 거주가 가능하면서 내진보강 공사를 실시할 수가 있으며, 접합부 시공성이 탁월하며, 특히 필요 내진보강량 산정이 간편한 전형적인 강도증진형으로 전단파괴가 지배적인 비내진상세를 가지는 국내 중 저층 철근콘크리트 건물에는 내력확보가 용이한 내진보강공법이다. 본 연구에서 제안한 SRCF 외부접합형 공법의 유용성을 검증 할 목적으로 기존 중 저층 철근콘크리트 학교건물의 골조를 대상으로 유사동적실험을 실시하여 내진보강효과를 검토하였다. 그 결과, 국내에서 발생 가능한 최대 지진규모(300gal)에서 비보강 골조는 붕괴를 하였지만, SRCF 외부접합형 내진보강법으로 보강한 골조는 경미한 지진피해가 예상되었으며, 대규모 지진(400, 500gal)을 상정한 경우에도 소규모 이하의 지진피해가 예상되어 본 연구에서 개발한 SRCF 내진보강법의 유효성이 검증되었다고 사료된다.

• Journal of Oral Medicine and Pain
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• 제45권2호
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• pp.23-28
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• 2020

Purpose: Temporomadibular disorders (TMDs) can result in chronic pain, which is often associated with psychological and sleep disturbance. Increased levels of psychological and sleep impairments are often related with poor treatment outcomes. The purpose of the present study was to evaluate clinical features, psychological profiles and the prevalence of clinical insomnia in TMD patients with chronic pain and to suggest an effective treatment approach. Methods: A total of 200 TMD patients who had visited the Pusan National University Hospital dental clinic for treatment of their pain were recruited from June 2018 through December 2019. TMD patients were classified into an acute (n=100) and chronic (n=100) group and compared the clinical symptoms. The primary diagnosis of TMD were categorized as TMD with joint pain group (TMD_J), TMD with muscle pain group (TMD_M) and TMD with joint-muscle mixed pain group (TMD_JM). Self- report measures of sleep quality and psychological profiles were evaluated via the Insomnia Severity Index (ISI) and the Korean Stress Response Inventory (SRI). Independent t-tests, Mann-Whitney U-tests, and chisquared test were used for the statistical analysis. Results: Chronic TMD patients showed higher pain intensity, as well as higher prevalence of related symptoms (headache, musculoskeletal pain) and myogenous pain. They also had significantly higher scores in all SRI parameters and a higher percentage of clinical insomnia than acute TMD patients. Conclusions: Based on the above results, psychological profiles and sleep quality assessments are necessary to provide essential data that will allow for improved treatment of chronic TMD patients.

• BMB Reports
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• 제37권4호
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• pp.439-444
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• 2004

Double stranded targets on the cDNA microarray contain representatives of both the coding and noncoding strands, which will introduce hybridization competition with probes. Here, the effect of double and single strands of targets on the signal intensity and the ratios of Cy5/Cy3 within the same slide were compared. The results show that single stranded targets can increase the hybridization efficiency without changing the Cy5/Cy3 ratio. Based on these results, a new strategy was established by generating cDNA targets with asymmetric PCR, instead of conventional PCR, to increase the sensitivity of the cDNA microarray. Furthermore, the feasibility of this approach was validated. The results indicate that the cDNA microarray system based on asymmetric PCR is more sensitive, with no decrease in the reliability and reproducibility as compared with that based on conventional symmetric PCR.

• Investigative Magnetic Resonance Imaging
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• 제19권4호
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• pp.212-217
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• 2015

Purpose: For a single time-point hyperpolarized $^{13}C$ magnetic resonance spectroscopy imaging (MRSI) of animal models, scan-time window after injecting substrates is critical in terms of signal-to-noise ratio (SNR) of downstream metabolites. Pre-scans of time-resolved magnetic resonance spectroscopy (MRS) can be performed to determine the scan-time window. In this study, based on two-site exchange model, protocol-specific simulation approaches were developed for $^{13}C$ MRSI and the optimal scan-time window was determined to maximize the SNR of downstream metabolites. Materials and Methods: The arterial input function and conversion rate constant from injected substrates (pyruvate) to downstream metabolite (lactate) were precalibrated, based on pre-scans of time-resolved MRS. MRSI was simulated using two-site exchange model with considerations of scan parameters of MRSI. Optimal scan-time window for mapping lactate was chosen from simulated lactate intensity maps. The performance was validated by multiple in vivo experiments of BALB/C nude mice with MDA-MB-231 breast tumor cells. As a comparison, MRSI were performed with other scan-time windows simply chosen from the lactate signal intensities of pre-scan time-resolved MRS. Results: The optimal scan timing for our animal models was determined by simulation, and was found to be 15 s after injection of the pyruvate. Compared to the simple approach, we observed that the lactate peak signal to noise ratio (PSNR) was increased by 230%. Conclusion: Optimal scan timing to measure downstream metabolites using hyperpolarized $^{13}C$ MRSI can be determined by the proposed protocol-specific simulation approaches.

• 약학회지
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• 제41권6호
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• pp.749-755
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• 1997

It has been reported that dose-dependent $Ca^{2+}$ increase by menadione in platelets could be measured by fluorescent dye, quin-2. The problems will be described here rel ating to measuring $Ca^{2+}$ in menadione-exposed platelets using fura-2 and fluo-3, widely used fluorescent indicators. Additions of menadione to fura-2 loaded platelets and their lysates resulted in marked reduction in fluorescence intensity at both 340nm ($Ca^{2+}$-unbound form) 380nm ($Ca^{2+}$-undbound form) excitation wavelengths. Fura-2 excitation spectra were overlapped with UV-visible absorption spectra of menadione, suggesting that light absorption by menadione itself could quench fluorescence generated by fura-2. Next approach was to use fluo-3 which has the higher wavelength (490nm) of excitation. Previous work demonstrated that treatment with probenecid to platelets was required to prevent fluo-3 dye leakage. However, probenecid itself was proven to be inadequate to measure the concentration of intracellular $Ca^{2+}$; by reducing menadione-induced cytotoxicity in platelets. Our results suggest that it is not feasible to measure $Ca^{2+}$ in platelets by using fura-2 and fluo-3 in the presence of probenecid, and cautions should be taken to measure changes of intracellular $Ca^{2+}$ levels by fluorescent dyes following chemical exposure.

• Journal of Radiation Protection and Research
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• 제43권1호
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• pp.10-19
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• 2018

Background: Monte Carlo (MC) simulation is the most accurate for calculating radiation dose distribution and determining patient dose. In MC simulations of the therapeutic accelerator, the characteristics of the initial electron must be precisely determined in order to achieve accurate simulations. However, It has been computation-, labor-, and time-intensive to predict the beam characteristics through predominantly empirical approach. The aim of this study was to analyze the relationships between electron beam parameters and dose distribution, with the goal of simplifying the MC commissioning process. Materials and Methods: The Varian Clinac 2300 IX machine was modeled with the Geant4 MC-toolkit. The percent depth dose (PDD) and lateral beam profiles were assessed according to initial electron beam parameters of mean energy, radial intensity distribution, and energy distribution. Results and Discussion: The PDD values increased on average by 4.36% when the mean energy increased from 5.6 MeV to 6.4 MeV. The PDD was also increased by 2.77% when the energy spread increased from 0 MeV to 1.019 MeV. In the lateral dose profile, increasing the beam radial width from 0 mm to 4 mm at the full width at half maximum resulted in a dose decrease of 8.42% on the average. The profile also decreased by 4.81% when the mean energy was increased from 5.6 MeV to 6.4 MeV. Of all tested parameters, electron mean energy had the greatest influence on dose distribution. The PDD and profile were calculated using parameters optimized and compared with the golden beam data. The maximum dose difference was assessed as less than 2%. Conclusion: The relationship between the initial electron and treatment beam quality investigated in this study can be used in Monte Carlo commissioning of medical linear accelerator model.