• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.377-383
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• 2011

The objective of this study was to examine effect of ethylene glycol for semen cryopreservation in Korean Jeju Black Bull. The semen was cryopreserved with extenders containing cryoprotectants (7% glycerol and 3%, 5%, 7% ethylene glycol) and packed to 0.5 ml straws. The semen straws were located above 3 cm of liquid nitrogen for 5 min, 5 cm for 10 min and 8 cm for 10 min. And then frozen straw was plunged into $LN_2$. Post-thawed sperm motility, viability and membrane integrity were significantly higher in 5% ethylene glycol ($72.5{\pm}5.00%$, $54.88{\pm}0.66%$ and $46.00{\pm}2.40%$; p<0.05). Motility and viability were similar between 7% glycerol and 5% ethylene glycol. However, the membrane integrity was significantly higher in 5% ethylene glycol ($34.69{\pm}4.64%$ vs $46.00{\pm}2.40%$; p<0.05). The viability and membrane integrity were significantly higher in 5 cm for 10 min and 8 cm for 10 min than 3 cm for 5 min (viability: $55.81{\pm}2.94$, $55.19{\pm}3.34$ vs $47.94{\pm}3.48%$; p<0.05 and membrane integrity: $44.94{\pm}3.51$, $46.06{\pm}2.25$ vs $40.38{\pm}1.03%$; p<0.05). The percentage of capacitated sperm assessed by CTC staining, percentage of F pattern was higher in 7% glycerol, 5% and 7% ethylene glycerol, and AR pattern was significantly higher in 3% ethylene glycol. F pattern was significantly increased in 5 cm for 10 min and 8 cm for 10 min (p<0.05), but AR pattern was significantly increased in 3 cm for 5 min (p<0.05).

• The Plant Pathology Journal
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• v.36 no.5
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• pp.385-397
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• 2020

The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C₂H₂ TF CgCrzA is localized in both cytoplasm and nucleus under standard condition, and it translocated from cytoplasm to nucleus in a calcineurin-dependent manner. Moreover, the ΔCgCrzA was hypersensitive to cell wall perturbing agents and showed severe cell wall integrity defects. Deletion of the CgCRZA inhibited the development of invasive structures and lost pathogenicity to plant hosts. Our results suggested that calcineurin-responsive TF CgCrzA was not only involved in regulating cell wall integrity, but also in morphogenesis and virulence in C. gloeosporioides.

• Korean Journal of Remote Sensing
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• v.40 no.3
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• pp.243-255
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• 2024

This study compares Static Terrestrial Laser Scanning (STLS)with the conventional Total Station (TS) method for the geometric assessment of cylindrical storage tanks. With the crucial need for maintaining tank integrity in the oil and gas industry, STLS and TS methods are evaluated for their efficacy in assessing tank deformations. Using STLS and TS, the roundness and verticality of two cylindrical tanks were examined. A deformation analysis based on American Petroleum Institute (API) standards was then provided. Key objectives included comparing the two methods according to API standards, evaluating the workflow for STLS point cloud processing, and presenting the pros and cons of the STLS method for tank geometric assessment. The study found that STLS, with its detailed and high-resolution data acquisition, offers a substantial advantage in having a comprehensive structural assessment over TS. However, STLS requires more processing time and prior knowledge about the data to tune certain parameters and achieve accurate assessment. The project outcomes intend to enhance industry professionals' understanding of applying STLS and TS to tank assessments, helping them choose the best method for their specific requirements.

• Journal of Satellite, Information and Communications
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• v.12 no.4
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• pp.72-77
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• 2017

The Satellite Based Augmentation System (SBAS) broadcasts to users integrity and correction information for Global Navigation Satellite System (GNSS) such as GPS and GLONASS using geostationary orbit (GEO) satellites. In accordance with the recommendation of the International Civilian Aeronautical Organization (ICAO) to introduce SBAS until 2025, a Korean SBAS system development / construction project is underway with the Ministry of Land, Transport and Maritime Affairs. Korea Augmentation Satellite System (KASS) is a high precision GPS correction system which is composed of KASS Reference Station (KRS), KASS Processing Station (KPS), KASS Uplink Station (KUS), KASS Control Station (KCS) and GEO satellites. In this paper, we provided the conceptual design of the KASS uplink station, which is composed of the Signal Generator Section (SGS) and the Radio-Frequency Section (RFS), and interface between the KASS ground sector and the GEO satellite.

• Journal of Positioning, Navigation, and Timing
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• v.12 no.4
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• pp.369-377
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• 2023

This paper presents the analysis results of navigation performance of Korea Augmentation Satellite System (KASS) test signals. Performance analysis was performed with Global Positioning System (GPS) and Satellite Based Augmentation System (SBAS) signals received from 7 KASS reference stations. And the performances were analyzed in terms of the signal strength, statistics for each SBAS message, coverage of ionospheric correction, accuracy, integrity, continuity, and availability. In addition, the navigation solutions provided by commercial receiver was analyzed and the performance experienced by general users was presented. Lastly, directions for further improvement of the KASS system were addressed. These performance analysis results can be used to confirm the feasibility of utilizing KASS in user applications.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2012.10a
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• pp.374-377
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• 2012

GNSS(Global Navigation Satellite System) based land transportation infrastructure system is consists of receiving station and central station. The functions of the central system include receiving station's data gathering and decoding, carrier correction and integrity information generated, transmission of data in real-time. In general, The central station architecture should take into account various important points relating to hardware/software of system, data archiving and checking, availability and continuity of operation, etc. There is a fundamental need for a generic design capable of being used in any situation. Also, There is need to develop an expandable and interoperable central station architecture. In this paper, the process of design and manufacture and verification will be introduced.

• Journal of Positioning, Navigation, and Timing
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• v.11 no.4
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• pp.269-277
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• 2022

In this paper, we overview the system development status of the national maritime precise point positioning-real-time kinematic (PPP-RTK) service in Korea, also known as the Precise POsitioning and INTegrity monitoring (POINT) system. The development of the POINT service began in 2020, and the open service is scheduled to start in 2025. The architecture of the POINT system is composed of three provider-side facilities-a reference station, monitoring station, and central control station-and one user-side receiver platform. Here, we propose the detailed functionality of each component considering unidirectional broadcasting of augmentation data. To meet the centimeter-level user positioning accuracy in maritime coverage, new reference stations were installed. Each reference station operates with a dual receiver and dual antenna to reduce the risk of malfunctioning, which can deteriorate the availability of the POINT service. The initial experimental results of a testbed from corrections generated from the testbed network, including newly installed reference stations, are presented. The results show that the horizontal and vertical accuracies satisfy 2.63 cm and 5.77 cm, respectively. For the purpose of (near) real-time broadcasting of POINT correction data, we designed a correction message format including satellite orbit, satellite clock, satellite signal bias, ionospheric delay, tropospheric delay, and coordinate transformation parameters. The (near) real-time experimental setup utilizing (near) real-time processing of testbed network data and the designed message format are proposed for future testing and verification of the system.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.2
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• pp.282-288
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• 2014

The DGPS reference station is a national infrastructure generating GPS correctional information and transmitting the signal for Differential GPS. Currently, Korea has applied and operated the software-based DGPS reference station as a standard of the next generation proposed by the USCG in order to improve the hardware-based DGPS reference system. However, software-based DGPS reference station proposed by USCG was changed in software method, only for form. There is no advantage to changing software-based because the most critical part of architecture has not been improved. In this paper, we have designed a new software-based marine DGPS station architecture that a reference station software and a monitor station were integrated. The new marine DGPS station architecture based on software is a more simplified structure than it used to be and can be utilized in the DGPS reference station.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.4
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• pp.824-830
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• 2010

In order to prepare for increasing performance requirement for Differential Global Navigation Satellite System (DGNSS) services of International Maritime Organization (IMO) and International Association of Lighthouse Authorities (IALA), this paper focuses on design of network-based Automatic Identification System (AIS) reference station system that can perform the functionality of Differential Global Positioning System (DGPS) reference station in an AIS base station system. AIS base station receives the differential corrections from the DGPS reference station, and it is not a method for transmitting the received differential corrections to onboard AIS units, but it is a method for generating the optimized differential corrections for onboard AIS units in AIS coverage. Therefore this paper proposes an algorithm for generating the differential corrections at AIS reference station, and performs the performance assessment of the proposed algorithm based on DGPS correction data measured from a DGPS reference station. Finally this paper discusses the test results and efficiency of the proposed system.

• Korean Journal of Poultry Science
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• v.40 no.3
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• pp.171-178
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• 2013

The purpose of this study was to evaluate the sperm viability, normal acrosome and mitochondrial activity in the frozen-thawed fowl semen by different cryoprotectants. The experiment was carried out on 10 sexually adult roosters of Ogye. The semen was collected twice a week and pooled semen was diluted 1:1 EK extender containing no cryoprotectant at $5^{\circ}C$. After equilibration for 30 minutes, diluted chicken semen was diluted 1:1 extender containing either 7% dimethylacetamide (DMA), 7% dimethylformamide (DMF) or 7.5% methylacetamide (MA) at final concentration and was put in 0.5 mL plastic straws and frozen for 30 minutes by exposure to liquid nitrogen vapor 4 cm above the surface of liquid nitrogen, followed by plunging into liquid nitrogen. Frozen semen was thawed in water bath at $5^{\circ}C$ for 2 minutes. For cytometric analysis, the frozen-thawed semen was diluted with EK extender to a final concentration of 90 million spermatozoa per mL. Sperm membrane integrity was evaluated as SYBR-14 and propidium iodide (PI). Acrosome integrity was assessed with fluorescein isothiocyanate-labeled PSA and PI. The percentage of mitochondrial function was estimated by using Rhodamine123 (R123) and PI. In conclusion, freezing rooster semen by using 7% DMF as cryoprotectant was significantly highest in rates of survival and mitochondrial function while its rate of damage of acrosome was significantly lowest. As a result, DMF is the cryoprotectant that has the lowest influences on sperm membranes and acrosome integrity. Therefore it could be used for freezing method of animal genetic conservation method for poultry diversity.