• BMB Reports
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• 제39권4호
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• pp.464-467
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• 2006

Recently, the nucleotide sequences of entire genomes became available. This information combined with older sequencing data discloses the exact chromosomal location of millions of nucleotide markers stored in the databases at NCBI, EMBO or DDBJ. Despite having resolved the intron/exon structures of all described genes within these genomes with a stroke of a pen, the sequencing data opens up other interesting possibilities. For example, the genomic mapping of the end sequences of the human, murine and rat BAC libraries generated at The Institute for Genomic Research (TIGR), reveals now the entire encompassed sequence of the inserts for more than a million of these clones. Since these clones are individually stored, they are now an invaluable source for experiments which depend on genomic DNA. Isolation of smaller fragments from such clones with standard methods is a time consuming process. We describe here a reliable one-step cloning technique to obtain a DNA fragment with a defined size and sequence from larger genomic clones in less than 48 hours using a standard vector with a multiple cloning site, and common restriction enzymes and equipment. The only prerequisites are the sequences of ends of the insert and of the underlying genome.

• 한국미생물·생명공학회지
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• 제23권5호
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• pp.550-555
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• 1995

A strain of Pseudomonas sp. isolated from soil was shown to produce a high level of extracellular endo-inulinase. In this work, the endo-inulinase gene (inu1) of the bacterial strain was cloned into the plasmid pBR322 by using EcoRI restriction endonuclease and E. coli HB101 as a host strain. One out of 7, 000 transformants obtained from the above cloning experiment formed a clear zone around its colony on the selective medium supplemented with 2.0% inulin after a prolonged incubation at 37$\circ$C and subsequent cold shock treatment. The functional clone was found to carry a recombinant plasmid (pKMG50) with a 3.7 kb genomic insert containing the genetic information for the inulinase activity. The inulinase from E. coli HB101/pKMG50 was proved to be an endo-acting enzyme and produced constitutively in the recombinant E. coli cells. Zymogram of the enzyme from the recombinant cells with inulin substrate indicated that the molecular mass of the active protein was 190 Kd, while that of the endo-inulinase from the Pseudomonas strain was 170 Kd. This size discrepancy suggested that the inulinase from the recombinant E. coli HB101 cells might be the initial product of translation, not the mature form produced in the strain of Pseudomonas sp..

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2008년도 춘계학술대회논문집
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• pp.33-41
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• 2008

Architectural acoustics design of Namsadang exclusive use theaters should be designed to utilize variously to performance space that can fill flavor and taste of Namsadang performance of the Namsadang six yards. Also, analyze special quality that is sound enemy who follow in sound-absorbing materials fare arrangement of innards that is design material of architectural acoustics laying stress on tradition, use purpose and disappointment size that Namsadang exclusive use theaters seeks on the basis of specific space theme that is experience, disappointment form, seat and passageway Wall and ceiling etc. research and sound and meaning of a character wave motion powerful engineering phenomenon and reduction reverberation loss that is happened from indoor manufacturing thing reduction SCALE model of oval structure research and background of AL composition absorbing material of perforate 25% to heighten acoustic absorptivity of practical use internal organs sound absorption material emir quality sound-absorbing materials insert and layer of air most suitable reverberation time of Namsadang exclusive use theaters that 2.2m volume is $42,218\;m^3$ to become 1.2Sec architectural acoustics design do.

• BMB Reports
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• 제38권5호
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• pp.507-516
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• 2005

Antimicrobial peptides (AMPs) have been isolated and characterized from tissues and organisms representing virtually every kingdom and phylum. Their amino acid composition, amphipathicity, cationic charge, and size allow them to attach to and insert into membrane bilayers to form pores by 'barrel-stave', 'carpet' or 'toroidal-pore' mechanisms. Although these models are helpful for defining mechanisms of AMP activity, their relevance to resolving how peptides damage and kill microorganisms still needs to be clarified. Moreover, many AMPs employ sophisticated and dynamic mechanisms of action to carry out their likely roles in antimicrobial host defense. Recently, it has been speculated that transmembrane pore formation is not the only mechanism of microbial killing by AMPs. In fact, several observations suggest that translocated AMPs can alter cytoplasmic membrane septum formation, reduce cell-wall, nucleic acid, and protein synthesis, and inhibit enzymatic activity. In this review, we present the structures of several AMPs as well as models of how AMPs induce pore formation. AMPs have received special attention as a possible alternative way to combat antibiotic-resistant bacterial strains. It may be possible to design synthetic AMPs with enhanced activity for microbial cells, especially those with antibiotic resistance, as well as synergistic effects with conventional antibiotic agents that lack cytotoxic or hemolytic activity.

• 한국공간정보시스템학회 논문지
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• 제9권1호
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• pp.31-43
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• 2007

센서 데이터는 대용량이며 지속적으로 발생하는 특징이 있다. 따라서 대용량의 센서 데이터로부터 특정 데이터를 효과적으로 검색하기 위해서는 색인의 개발이 요구된다. 센서 데이터 색인은 데이터 아카이빙을 지원하기 위해서 일정한 시간이 지난 과거의 데이터를 효과적으로 삭제할 수 있어야 한다. 본 논문에서는 대용량 센서 데이터 아카이빙을 지원하기 위해 색인 분할 기법을 제안하고 구현하였다. 분할된 각각의 색인들은 가상색인으로 구성되어, 외부에서는 하나의 색인으로 보인다. 실험 결과 색인의 생성비용은 총 100,000번의 삽입 연산에 대해서 최대 8%의 성능 향상을 보였으며, 삽입되는 데이터의 개수가 많아질수록 성능이 더 향상됨을 보였다. 영역질의의 경우 각 질의영역이 적을수록, 시간도메인의 크기가 커질수록 큰 성능 향상을 보였다.

• 한국가축번식학회지
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• 제17권1호
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• pp.43-48
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• 1993

These experiments were carried out to construct mouse testis cDNA library and to to seen H-Y Ag gene. Mouse testis was obtained from BALB/c inbreed mouse that was after-born 1 week. Isolation of mouse testis total RNA was carried out by guanidum/cesium choloride, poly(A+) mRNAs were purified by oligo d(T)-cellulose chromatography method. To investigate protein synthesis activity, in-vitro translation carried out by total RNA and poly(A+) mRNA. The products of in-vitro translation were identified in 12.5% PAGE. Single strand DNA and double strand DNA were synthesized from poly(A+) mRNA and purified using phenol/chloroform/isoamylalcohol. Synthesized cDNA was combined with cohesive Eco RI polylinker, its recombination efficiencies were identified by X-gal and IPTG. In the cDNA library, 1$\times$107 phagemids were screened with 32P labelled probe. Hybridization were carried on $65^{\circ}C$ for 16~20hours. And 1$\times$106 phagemids were screened with rabbit-anti-H-Y. In former, select 5 positive clones, and later, 1 positive clone. Its southern blot analysis showed various size of insert cDNA from 0.7kb to 3kb.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2002년도 금형가공 심포지엄
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• pp.253-259
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• 2002

Micro cell structures with high aspect ratio were fabricated by injection molding method. The mold inserts had dimension $1.9cm\times8.3cm$ composed of a lot of micro posts and were fabricated by LIGA process. The size of the micro posts was $157{\mu}m\times157{\mu}m\times500{\mu}m$ and the gaps between two adjacent posts were $50{\mu}m$. Using Polymethylmethacrylate (PMMA) injection molding was performed. The key experimental variables were temperature, pressure, and time. By controlling these, good shaped mim cell structures with $50{\mu}m$ in wall thickness and $500{\mu}m$ in depth were obtained. In order to understand micro molding mechanism, shape changes of molded PMMA were studied with experimental variables. And the durability of mold insert was investigated, too. The results show that the most important factor in molding processes was the mold temperature that is closely related to the filling of the melt into the micro cavity. And the holding time before cooling showed a great effect on the quality of molded PMMA.

• ETRI Journal
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• 제36권6호
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• pp.931-941
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• 2014

Many methodologies for clock mesh networks have been introduced for two-dimensional integrated circuit clock distribution networks, such as methods to reduce the total wirelength for power consumption and to reduce the clock skew variation through consideration of buffer placement and sizing. In this paper, we present a methodology for clock mesh to reduce both the clock skew and the total wirelength in three-dimensional integrated circuits. To reduce the total wirelength, we construct a smaller mesh size on a die where the clock source is not directly connected. We also insert through-silicon vias (TSVs) to distribute the clock signal using an effective clock TSV insertion algorithm, which can reduce the total wirelength on each die. The results of our proposed methods show that the total wirelength was reduced by 12.2%, the clock skew by 16.11%, and the clock skew variation by 11.74%, on average. These advantages are possible through increasing the buffer area by 2.49% on the benchmark circuits.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2003년도 제40회 국제학술심포지움
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• pp.14-27
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• 2003

The genomes of Arabidopsis and rice have been fully sequenced. Genomic sequencing provides global information about genome structure and organization. A comprehensive research account of our recent studies conducted on genome painting, comparative genomics and genome fusion is provided in order to project the prospects of plant cytogenetic research in post-genomics era. Genome analysis by GISH using genome painting is demonstrated as an excellent means suitable for visualization of a whole genome, since total genomic DNA representing the overall molecular composition of the genome is used as a probe. FISH on extended DNA fibers has been developed for high-resolution FISH and has contributed to determining the copy number and order of genes. We have also mapped a number of genes involving starch synthesis on wheat chromosomes by FISH and compared the position of these genes on linkage map of rice. Macro synteny between wheat and rice can be observed by comparing the location of these genes in spite of the fact that the size of DNA per chromosome differs by 20 fold in two. Moreover, to approach our goal towards making bread and udon noodles from rice flour in future by incorporating bread making and the noodle qualifies in rice, we have been successful in introducing large genomic DNA fragments containing agronomically important genes of wheat into a rice by successive introduction of large insert BAC clones, there by expanding genetic variability in rice. We call this method genome fusion.

• 한국정보처리학회논문지
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• 제7권3호
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• pp.935-942
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• 2000

In this paper we introduce a fast simplification algorithm for terrain height fields to produce a triangulated irregular network, based on the greedy insertion algorithm in [1,4,5]. Our algorithm partitions a terrain height data into rectangular blocks with the same size ad simplifies blocks one by one with the greedy insertion algorithm. Our algorithm references only to the points and the triangles withing each current block for adding a point into the triangulation. Therefore, the algorithm runs faster than the greedy insertion algorithm, which references all input points and triangles in the terrain. Our experiment shows that partitioning method runs from 4 to more than 20 times faster, and it approximates test height fields as accurately as the greedy insertion algorithms. Most greedy insertion algorithms suffer from elongated triangles that usually appear near the boundaries. However, we insert the four corner points into each block to produce the base triangulation of the block before the point addition step begins so that elongated triangles could not appear in th simplified terrain.