• 한국작물학회지
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• 제31권3호
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• pp.326-335
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• 1986

본 시험을 생식생장기 냉수관개가 벼의 Source와 Sink 관련형질 및 양분흡수에 미치는 시비반응을 구명한 것으로 그 결과를 요약하면 다음과 같다. 1. 질소의 과용은 생육 및 수량을 감퇴시켰으나 인산, 가리, 규산의 증비로 생육량의 증대, 영화착생량과 등숙비율 향상에 유리하였다. 2. 영화의 착생수, 기경과 영화의 퇴화율, 불임율은 인산, 가리, 규산의 증비로 그 피해를 경감시킬 수 있었으며 특히 규산의 증비효과가 현저하였고 지경당 개화는 1차 지경보다 2차 지경에 착생한 영화수에 크게 영향을 받았다. 3. 미립 및 등미의 발생량과 수량과는 부의 상관관계가 있었으며 인산, 가리, 규산의 증비는 추액및 서미의 발생억제와 수량증가에 효과적이었다. 4. 질소의 증비는 엽신. 지경, 영곡의 전질소함량을 높이고 인산, 가리, 규산의 흡수를 저해시켰으나 인산, 가리, 규산의 증비는 질소의 과잉흡수억제 및 인산, 가리, 규산의 흡수를 증가시켰고 지경의 무기성분함양은 엽신보다는 적었다. 5. 엽신. 지경, 영곡의 무기성분함량의 상호관련성은 지경의 무기성분은 엽신의 무기성분함양에 영향을 받고 지경은 다시 영곡의 무기성분조성에 영향을 미치고 있음을 알 수 있었다. 6. Source 와 Sink 관련형질에 미치는 엽신, 지경, 영곡의 무기성분반응을 보면 지경과 영화의 퇴화율, 불임율, 등숙비율, 수량 모두 엽신, 영곡보다 지경의 무기성분함양에 크게 영향을 받았다. 이로 보아 지경은 단순한 양분이동의 통로기능만을 갖는 것이 아니라 종실의 물질집적에 중요한 생리적 기능을 가지는 기관임을 알 수 있었다.

• 한국토양비료학회지
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• 제45권6호
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• pp.998-1003
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• 2012

Pantoea agglomerans DSM3493 was isolated from green house soils collected from Chungchugnam-do province, Gongju-Gun area in South Korea and phosphate solubilization and organic acid production of the strain were assessed using three types of insoluble phosphate sources (Ca phosphate, Fe phosphate and Al phosphate) under three different pH conditions (7, 8 and 9). The highest Ca phosphate solubilization ($651{\mu}g\;mL^{-1}$) was recorded at pH 7 followed by pH 8 and 9 (428 and $424{\mu}g\;mL^{-1}$ respectively). The solubilization rate was found to be 80.4, 98.1 and $88.7{\mu}g\;mL^{-1}$ (for Fe phosphate containing medium) and 9.3, 12.1 and $29.8{\mu}g\;mL^{-1}$ (for the Al phosphate containing medium) respectively at pH 7, 8 and 9. Though increasing pH of the medium caused reduction in the rate of solubilization of Ca phosphate, solubilization of Fe and Al phosphates enhanced with increasing pH. By contrast, the highest amount of organic acid was produced with Ca phosphate while the lowest was recorded with the presence of Al phosphate. Among the organic acids, gluconic acid production was found to be the highest, followed by oxalic acid and citric acid regardless the source of phosphate. Results can thus be concluded that the production of organic acids appears to play a significant role in the inorganic phosphate solubilization.

• Food Science and Biotechnology
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• 제17권4호
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• pp.794-798
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• 2008

Guar gum and $\kappa$-carrageenan were investigated as a substitutes for phosphate in pork meat processing. Emulsion-type pork sausages were prepared in which 0.5% phosphate was used for the control, and either $\kappa$-carrageenan or guar gum were added at levels of 0.1 or 0.5% for comparison. The hydrocolloid compounds significantly enhanced water holding capacity and cooking loss. However, hardness, cohesiveness, and chewiness were not well-maintained when compared to the control; this was attributable to the altered water distribution as well as enhanced water holding capacity of the sausages by the addition of $\kappa$-carrageenan and guar gum. Furthermore, the phosphate-free sausages had similar storage stability as the phosphate-added sausage. Overall, the results suggest that $\kappa$-carrageenan or guar gum can be used in place of phosphate in conventional processing to successfully prepare phosphate-tree pork sausages.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1385-1389
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• 2007

5-Enolpyruvylshikimate-3-phosphate (EPSP) synthase catalyzes the formation of EPSP and inorganic phosphate from shikimate-3-phosphate (S3P) and phosphoenolpyruvate (PEP) in the biosynthesis of aromatic amino acids. To delineate the domain-specific function, we successfully isolated the discontinuous C-terminal domain (residues 1-21, linkers, 240-427) of EPSP synthase (427 residues) by site-directed mutagenesis. The engineered C-terminal domains containing no linker (CTD), or with gly-gly ($CTD^{GG}$) and gly-ser-ser-gly ($CTD^{GSSG}$) linkers were purified and characterized as having distinct native-like secondary and tertiary structures. However, isothermal titration calorimetry (ITC), $^{15}N-HSQC$,\;and\;^{31}P-NMR$ revealed that neither its substrate nor inhibitor binds the isolated domain. The isolated domain maintained structural integrity, but did not function as the half of the full-length protein.

• 한국결정성장학회지
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• 제4권1호
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• pp.105-110
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• 1994

L-arginine phosphate monohydrate (LAP)와 tris(hydroxymethyl)aminomethane sulfate(THAMS)는 레이저의 파장변환소자등과 같은 소자응용에 있어서 새로운 유기 비선형광학재료이다. 본 연구에서는 온도강하법과 온도차법을 이용하여 LAP와 THAMS 단결정의 육성을 시도하였으며 이들 방벙에 따른 육성조건을 확립하였다. 그리고 육성결정의 특성을 광학현미경, XPD, FTIR 등을 통하여 평가하였다.

• 한국수산과학회지
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• 제40권2호
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• pp.95-101
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• 2007

We investigated the growth and phosphate uptake of a toxic dinoflagellate, Gymnodinium catenatum, isolated from Yeosuhae Bay, South Korea. A short-term phosphate uptake experiment revealed that its maximum uptake and the half-saturation constant were 1.39 pmol/cell/hr and $2.65{\mu}M$, respectively. In a semicontinuous culture, the maximum specific growth rate and minimum phosphorus cell quota of G. catenatum were 0.39/day and 1.27 pmol/cell, respectively. Thus, G. catenatum is a poor competitor in terms of inorganic nutrient use and is unlikely to form blooms in Yeosuhae Bay.

• The Korean Journal of Physiology and Pharmacology
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• 제11권2호
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• pp.71-77
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• 2007

Cisplatin treatment increases the excretion of inorganic phosphate in vivo. However, the mechanism by which cisplatin reduces phosphate uptake through renal proximal tubular cells has not yet been elucidated. We examined the effect of cisplatin on $Na^+$-dependent phosphate uptake in opossum kidney (OK) cells, an established proximal tubular cell line. Cells were exposed to cisplatin for an appropriate time period and phosphate uptake was measured using $[^{32}P]$-phosphate. Changes in the number of phosphate transporter in membranes were evaluated by kinetic analysis, $[^{14}C]$phosphonoformic acid binding, and Western blot analysis. Cisplatin inhibited phosphate uptake in a time- and dose-dependent manner, and also the $Na^+$-dependent uptake without altering $Na^+$-independent uptake. The cisplatin inhibition was not affected by the hydrogen peroxide scavenger catalase, but completely prevented by the hydroxyl radical scavenger dimethylthiourea. Antioxidants were ineffective in preventing the cisplatin-induced inhibition of phosphate uptake. Kinetic analysis indicated that cisplatin decreased Vmax of $Na^+$-dependent phosphate uptake without any change in the Km value. $Na^+$-dependent phosphonoformic acid binding was decreased by cisplatin treatment. Western blot analysis showed that cisplatin caused degradation of $Na^+$-dependent phosphate transporter protein. Taken together, these data suggest that cisplatin inhibits phosphate transport in renal proximal tubular cells through the reduction in the number of functional phosphate transport units. Such effects of cisplatin are mediated by production of hydroxyl radicals.

• Journal of Ginseng Research
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• 제35권1호
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• pp.12-20
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• 2011

The characteristics of absorption and accumulation of inorganic germanium in Panax ginseng C. A. Meyer were examined. In 4-year-old P. ginseng, the germanium content of the field soil increased with increased amounts and frequencies of inorganic germanium application, while chemical components of the soil, such as available phosphate and exchangeable calcium, potassium, and magnesium, decreased with the increased inorganic germanium application. In the 4-year-old P. ginseng, the germanium content was highest in the rhizome and increased in the order of stem, leaf, lateral root, and main root, suggesting that inorganic germanium was absorbed from the root and translocated to the stem and leaf via the rhizome. As for changes in ginsenosides in 4-year-old P. ginseng rhizomes, the contents of ginsenosides $Rb_1$, $Rb_2$, Re, and Rf decreased as the germanium content in soil increased. Ginsenosides $Rb_1$, $Rb_2$, Rc, Re, and Rf in the main root also decreased with increasing germanium content in the main root. The results suggest that inorganic germanium treatment may increase organic germanium in harvested P. ginseng, thus enhancing the medicinal effi cacy of ginseng products.

• 한국축산식품학회지
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• 제38권1호
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• pp.26-42
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• 2018

Today incorporation of natural ingredients as inorganic phosphate replacers has come into prominence as a novel research topic due to health concerns about phosphates. In this study, we aimed to investigate the quality of emulsified chicken meatballs produced with Jerusalem artichoke powder (JAP), either alone or in combination with sodium carbonate (SC) as sodium tripolyphosphate (STPP) replacers. The results showed that naturally dried JAP showed favorable technological properties in terms of water-oil binding and gelling. Emulsion batters formulated with JAP-SC mixture showed lower jelly and fat separation, higher water-holding capacity and higher emulsion stability than control samples with STPP. In final product, incorporation of JAP-SC mixture increased moisture and reduced lipid and energy values, and kept the pH value similar to control. Added JAP lead to increments in $b^*$ values whereas decreases $L^*$ values. Cook yield was similar to control in phosphate-free samples formulated with JAP-SC mix. Either low or medium ratios of JAP in combination with SC managed to protect most of the sensory parameters, while sensory scores tend to decrease in samples containing high levels of JAP. Addition of JAP to formulations presented samples that have equivalent behavior to phosphates in terms of lipid oxidation. In conclusion, our study confirms that utilization of JAP in combination with SC had promising effects as phosphate replacers by presenting natural solutions and providing equivalent quality to standard phosphate containing products.

• Molecules and Cells
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• 제40권10호
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• pp.697-705
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• 2017

The maintenance of inorganic phosphate (Pi) homeostasis is essential for plant growth and yield. Plants have evolved strategies to cope with Pi starvation at the transcriptional, post-transcriptional, and post-translational levels, which maximizes its availability. Many transcription factors, miRNAs, and transporters participate in the Pi starvation signaling pathway where their activities are modulated by sugar and phytohormone signaling. Environmental stresses significantly affect the uptake and utilization of nutrients by plants, but their effects on the Pi starvation response remain unclear. Recently, we reported that Pi starvation signaling is affected by abiotic stresses such as salt, abscisic acid, and drought. In this review, we identified transcription factors, such as MYB, WRKY, and zinc finger transcription factors with functions in Pi starvation and other environmental stress signaling. In silico analysis of the promoter regions of Pi starvation-responsive genes, including phosphate transporters, microRNAs, and phosphate starvation-induced genes, suggest that their expression may be regulated by other environmental stresses, such as hormones, drought, cold, heat, and pathogens as well as by Pi starvation. Thus, we suggest the possibility of cross-talk between Pi starvation signaling and other environmental stress signaling pathways.