• Asian-Australasian Journal of Animal Sciences
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• v.9 no.5
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• pp.557-562
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• 1996

The effects of diets differing in protein content through soybean meal supplementation on ruminal fractionation of selenium (Se) were studied. A $3{\times}3$ Latin square design was used with three Japanese Corriedale wethers (45 kg average body weight), three periods, and three dietary treatment. The three dietary treatments were : Diet 1, without soybean meal supplementation (14% crude protein, CP); Diet 2, with 10% soybean meal supplementation (16.5% CP); and Diet 3, with 20% soybean meal supplementation (19% CP). All the diets had a Se supplementation in the form of sodium selenite at 0.2 mg Se/kg dietary DM. The Se supplement and the concentrate mixture were fed only in the morning before the hay was given. Daily feeding schedule for gay was set at 09:00 and 17:00 h. On the final day of collection period, ruminal fluid samples were obtained at 0.5, 2, 6, 12 and 24 h post-feeding starting at 09:00 h. Total ruminal fluid Se was markedly higher (p<0.05) in Diet 3 than those in Diets 1 and 2 at almost all sampling time except at 24 h. The proportion of Se in soluble protein to the total ruminal Se was higher (p< 0.05) in Diet 3 (40%) followed by Diet 2 (28%) and Diet 1 (21%). The proportion of free inorganic Se to the total ruminal Se was the reverse, especially after two hours where Diet 1 (p<0.05) was higher than the other diets. Bacterial Se was lower (p < 0.05) in Diet 1 than those in Diets 2 and 3 at any sampling time. The highest was observed at 2 h postprandially in all diets with a value of 421, 556, $655{\mu}g/kg$ bacterial DM for Diet 1, 2 and 3, respectively. No differences (p>0.05) were observed on ruminal pH, ammonia and total nolatile fatty acids although increasing protein supplementation tended to decline the ruminal pH and increase ruminal ammonia. This study concludes that increasing dietary protein content by soybean meal supplementation can affect the ruminal Se metabolism.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.284-288
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• 2005

This study was conducted to clarify the effects of supplying methods of selenium on the growth and Se uptake of hydroponically grown tomato plants. Tomato seeds (Lycopersicum esculentum Mill. cv. Momotaro T-93, Daki Seed Co.) were sown in plug tray with fifty holes, and raised for sixty days. Tomato seedlings transplanted to coco fiber slabs were supplied with the nutrient solutions adjusted to EC $2.3dS{\cdot}m^{-1}$ and pH $5.8\~6.2$ recommended by the Japanese Horticultural Experiment Station. Selenium forms used were inorganic $SeO_2$ (here in after referred to Se) and organic selenium chlenium with sugar fatty acid ester (here in after referred to chelated-Se). 10 ppm selenium solutions were treated to tomato plants with foliar applications, drenching, and foliar application plus drenching. Growth characteristics in terms of plant height, number of leaves, leaf area and chlorophyll content were significantly increased in the plot of foliar application ot Se, and in the plot of foliar application plus drenching of chelated-Se than other plots, respectively. Transported contents of selenium into the tomato fruits were highest as 0.302 ppm in the plot of foliar application plus drenching of chelated-Se. Also, it had tended to be higher in the plot of foliar application plus drenching than in the plots of foliar application or drenching in both of Se and chelated-Se. Foliar application and drenching of organic chelated-Se were effective to produce the functional tomato fruits.

• Analytical Science and Technology
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• v.26 no.3
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• pp.182-189
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• 2013

The purpose of this research is to separate and quantitate selenium species in some food samples with HPLC-ICP-MS. Cation exchange chromatography showed efficient separation only for inorganic Se species while reversed phase ion pair chromatography showed good separation for both inorganic and organic Se species. $C_8$ column ($Symmetryshield^{TM}\;RP_8$, 3.5 ${\mu}m$, $4.6{\times}150$ mm) was used with optimum condition of 5% methanol mobile phase, 0.05% of nonafluorovaleric acid ion pairing reagent. Five standard Se species of Se(IV), Se(VI), SeCys(selenocystein), SeMet(selenomethionine) and Se-M-C(seleno methyl cystein) were separated successfully under the optimum condition (mobile phase; 5% methanol, ion-pairing reagent; 0.05% nonafluorovaleric acid, flow rate; 0.9 mL $min^{-1}$). To extract Se species, microwave assisted and enzyme-assisted extraction methods were studied. In enzyme-assisted extraction method, protease I for garlic, protease I plus trypsin for pork and mackerel, and protease XIV for tuna showed the best extraction efficiency. With the optimum condition for each sample, it was found that mostly inorganic Se, SeCys and SeMet are present in the sample studied ranging from few ${\mu}g$ $g^{-1}$ to few tens of ${\mu}g$ $g^{-1}$.

• Korean Journal of Poultry Science
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• v.36 no.2
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• pp.103-110
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• 2009

This experiment was conducted using 350 Lohmann Brown layers (67 weeks of age) to evaluate the dietary effect of organic or inorganic Se and Cu on their contents in chicken eggs. The layers were divided into seven groups and fed a commercial diet or one of the six experimental diets containing different levels of Se and Cu (T1, 0.3ppm organic Se; T2, 1.0ppm organic Se; T3, 1.0ppm inorganic Se; T4, 125ppm organic Cu; T5, 250ppm organic Cu; and T6, 250ppm inorganic Cu) for 5 weeks. No significant differences were observed in egg production and its qualities among groups. The contents of blood cholesterol in the birds fed various levels of Se and Cu were not significantly different as compared to control. Se contents in eggs were linearly increased as dietary Se levels increased for both sources, but Se contents from the groups fed organic Se were slightly higher than those fed inorganic Se. Sensory characteristics of eggs were not influenced by dietary treatments. In conclusion, Se contents in eggs were linearly increased as dietary levels of organic or inorganic Se increased, but eggs in layers fed the diet containing organic Se did not show higher Se contents than those in birds fed dietary inorganic Se.

• Animal Bioscience
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• v.34 no.11
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• pp.1811-1821
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• 2021

Objective: A trial was conducted to investigate the effects of supplemental levels of Mn provided by organic and inorganic trace mineral supplements on growth, tissue mineralization, mineral balance, and antioxidant status of growing broiler chicks. Methods: A total of 500 male chicks (8-d-old) were used in 10-day feeding trial, with 10 treatments and 10 replicates of 5 chicks per treatment. A 2×5 factorial design was used where supplemental Mn levels (0, 25, 50, 75, and 100 mg Mn/kg diet) were provided as MnSO₄·H₂O or MnPro. When Mn was supplied as MnPro, supplements of zinc, copper, iron, and selenium were supplied as organic minerals, whereas in MnSO₄·H₂O supplemented diets, inorganic salts were used as sources of other trace minerals. Performance data were fitted to a linearbroken line regression model to estimate the optimal supplemental Mn levels. Results: Manganese supplementation improved body weight, average daily gain (ADG) and feed conversion ratio (FCR) compared with chicks fed diets not supplemented with Mn. Manganese in liver, breast muscle, and tibia were greatest at 50, 75, and 100 mg supplemental Mn/kg diet, respectively. Higher activities of glutathione peroxidase and superoxide dismutase (total-SOD) were found in both liver and breast muscle of chicks fed diets supplemented with inorganic minerals. In chicks fed MnSO₄·H₂O, ADG, FCR, Mn balance, and concentration in liver were optimized at 59.8, 74.3, 20.6, and 43.1 mg supplemental Mn/kg diet, respectively. In MnPro fed chicks, ADG, FCR, Mn balance, and concentration in liver and breast were optimized at 20.6, 38.0, 16.6, 33.5, and 62.3 mg supplemental Mn/kg, respectively. Conclusion: Lower levels of organic Mn were required by growing chicks for performance optimization compared to inorganic Mn. Based on the FCR, the ideal supplemental levels of organic and inorganic Mn in chick feeds were 38.0 and 74.3 mg Mn/kg diet, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.2
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• pp.243-249
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• 2001

Thirty six barrows with an initial body weight of 28 kg were used to determine the effect of two dietary Se sources and a wide range of Se levels encompassing 0.3, 1.0, 3.0, 5.0, 7.0, and 10.0 mg/kg Se. The organic Se form was a Se-enriched yeast product, whereas the inorganic Se source was sodium selenite. The experiment was a $2{\times}6$ RCB design conducted in three replicates. Each barrow was placed in an individual metabolism crate and provided their dietary treatment and water on an ad libitum basis for a minimum 2 wk period, whereupon feed intake was adjusted to a constant intake within replicate at approximately 90% of intake for a 4 d adjustment period. Urine and feces were subsequently collected for a 7 d period and analyzed for Se and minerals. The results demonstrated that urinary Se was approximately 25% higher when pigs were fed sodium selenite (p<0.01), whereas fecal Se was lower by 25% (p<0.01). Se retention tended to be higher when organic Se was provided (p>0.15). Urinary Se increased as dietary Se level increased for both Se sources but increased more and at a high rate when sodium selenite was fed resulting in an interaction response (p<0.01). Fecal Se increased linearly as the dietary level of both Se sources increased, but the fecal Se from organic Se increased at a faster rate resulting in an interaction response (p<0.01). Se retention increased linearly (p<0.01) as dietary Se increased for both Se sources. The apparent digestibility of Se increased by Se level when pigs were fed sodium selenite, but not when the organic Se source was provided resulting in an interaction response (p<0.05). Retention of consumed Ca, Zn increased when pigs were fed organic Se (p<0.05) whereas P and Na retention were higher when the inorganic Se was provided. Mineral retention was not affected by dietary Se level except P. These results suggest that Se excretion by urine was the main route of excretion when pigs were fed sodium selenite but the fecal route when Se-enriched yeast was provided. The excretion of Fe, Zn, Mn, and Cu via urine and feces was not affected by high dietary Se level or dietary Se sources.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.2
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• pp.229-236
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• 2007

This study was conducted to determine effects of different selenium (Se) sources on performance, carcass characteristics, blood measures (whole blood Se concentration and plasma glutathione peroxidase (GSH-Px) activity), and Se concentrations in tissues of finishing Hanwoo steers (Korean native steers). Twenty finishing Hanwoo steers (average body weight=536${\pm}$23.4 kg, average age=approximately 20 months) were allotted to treatments in four groups of five steers per pen for 16 weeks preceding slaughter. Treatments were control (CON), spent mushroom composts from Se-enriched mushrooms (Se-SMC), selenized yeast (Se-Y), and sodium selenite (SS). Dietary Se levels of all treatments except CON were 0.9 mg Se/kg on the dry matter basis. Body weight was measured at the first and final day of trial, and blood samples were collected to analyze whole blood Se concentration and plasma GSH-Px activity at 2, 4, 8, and 16 weeks. At the end of trial, steers were slaughtered to collect muscle and liver samples for their Se analyses, and carcass data were recorded. In terms of dry matter intake, body weight gain and carcass characteristics, no significant differences among treatments were observed. Whole blood Se concentrations were significantly higher (p<0.05) for Se-SMC and Se-Y treatments than for CON at each collection period, with no significant difference between SS and CON. For weeks 2 and 8, there was no significant difference for whole blood Se concentration between Se-SMC and Se-Y, but for weeks 4 and 16, Se-Y treatments were significantly higher (p<0.05) than Se-SMC. No differences were observed for plasma GSH-Px activity between Se-SMC and Se-Y. The Se concentrations in hind leg and liver were significantly different among treatments (p<0.05) and those in both tissues ranked the greatest in Se-Y, followed by Se-SMC, SS, and CON treatments. However, tissue Se concentration for SS was not different from that for CON. These results showed that feeding organic Se sources such as Se-SMC and Se-Y enhanced Se concentration in tissues, while SS, the most common supplement of inorganic Se, was inefficient in Se deposition. Even though Se-Y had a higher Se concentration in tissues than Se-SMC, replacing Se-Y with Se-SMC in diets of beef steers would be an inexpensive way to increase Se concentration in beef.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.8 no.2
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• pp.196-208
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• 1998

To improve the quality of environmental measurements and evaluation of the workplace air in the pigment manufacturing industries, we analyzed metal(chromium, cadmium, lead, iron, cobalt, manganese, antimony, titanium, arsenic, and selenium) concentrations by ICP-AES in sixty seven samples of inorganic bulk pigments which are produced and/or used in Korea. We also collected MSDS which has to be supplied by manufacturer and/or supplier and posted in the workplace according to the Hazard Communication Standards, and compared the number of metals listed in each MSDS with the number of metals determined by ICP-AES. Results were as followed; 1. Among seventeen yellowish-colored samples, chromium(2~19%) and lead(0.1~61%) were the two major metals. In thirteen reddish-colored samples, iron was the major component with 37~81%. Cobalt and manganese were detected in blue-colored samples with less than 1%, while antimony and titanium were the major two metals in white-colored pigments with 178~300 ppm and with 36~65%, respectively. 2. In area samples collected in workplace air(one pigments producing factory and five retailer stores), iron and manganese were detected but the concentrations not exceeded the TLVs(1 and $5mg/m^3$, respectively). In three of fifteen samples, the concentrations of lead exceeded the TLV ($0.05mg/m^3$). 3. Two out of seven companies provided MSDS, and the average provision rate was 22.4%. And the coincidence rate of the number of metals referenced in MSDS and determined by ICP-AES mostly accorded, but in one sample, different metal was detected from MSDS. In summary, metals have to be concerned in evaluation of the workplace air dealing with compounds of inorganic pigments dust are cobalt, chromium, iron, manganese, lead and antimony, and these are simultaneously determined by ICP-AES. Taking this opportunity, it is needed to reinforce that the personnel is to be concerned about prevention of workers' ill health regarding to provision of MSDS.

• Food Science of Animal Resources
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• v.26 no.2
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• pp.197-203
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• 2006

This study was conducted to investigate the effect of seleium (Se) and vitamin E supplementation on meat quality and shelf-life in finishing pigs. A total of eighty ($Landrace{\times}Yorkshire{\times}Duroc$) pigs (74.74 initial BW) were randomly allocated into five treatments with four replications and fed for six weeks. Dietary treatments included 1) CON (basal diet), 2) ISE2 (basal diet+0.2 ppm inorganic Se+100 ppm vitamin E), 3) ISE4 (basal diet+0.4 ppm inorganic Se+100 ppm vitamin E), 4) OSE2 (basal diet+0.2 ppm organic Se+100 ppm vitamin E) and 5) OSE4 (basal diet+0.4 ppm organic Se+100 ppm vitamin E). Cooking loss was decreased in treatment of ISE4 as compared with treatment of CON (p<0.05). Crude fat (%) of OSE2 was higher than those of others (p<0.05). Redness ($a^*$- value) of M. longissimus dorsi was increased in treatment of OSE2 as compared with other treatments after 100 of storage (p<0.05). Thiobarbituric acid reactive substances (TBARS) was decreased in treatment of OSE4 compared with treatments of CON and ISE2 after 10d of storage (p<0.05). In conclusion, Se and vitamin E combination did not significantly affect cooking loss,$a^*$- value and TBARS except for cases.

• Journal of environmental and Sanitary engineering
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• v.6 no.2
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• pp.17-31
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• 1991

The effects of environmental agents on health are great concern for all. It was recognized that each human has differential susceptibility to environmental effects. Susceptibility are changed by many factors includin gdevelopmpntal processes, genetic factors, nutritional stratus, preexisting disease conditions, life style and personal habits. Of all factors nutritional factors seem to be the area most modifiable. Consequently, It is an area that must be more thoroughly evaluated. In this paper, nutrient and environment interactions are reviewed briefly with published literatures. This paper deals with the influence of micronutrients(energy, protein and fat), Vitamins (vitamin 4, vitamin B-complex, vitamin C, vitamin D and vitamin I) and Minerals(calcium, iron, selenium, zinc and other minerls) on environmental effects. The role of arch nutrient was assessed in modifyine the expression of environmental pollutant toxicity with available litertures. In each nutrient section, the effect of environment was considered in following agents : heavy metals(lead, cadmium, mercury, silver and etc), inorganic agents(nitwits, sulfite, fluoride and etc), organic agents(benzene, carbon tatra-chloride, aflatoxin, auto dye, dialbrin etc), Irritant gas(ozone, carbon monooxide and etc), physical agents(X-irradiation, ultra violet, temperature and noise) and insectcides. The extent to which nutritional status modifies environmental effects 3nd its converse, how envirollments affects nutritional status is very complex. In deed, at the present time there are more than 50 chelnical/phycical agents that affect the nutrient metabolism and/or have their toxicity either directly diminished or enhanced by nutrients of those agents, small number of agents for each nutrients have sufficient evidence to warrant any reasonable degree of confidence in their hypothesized associtation. With these information at this present time it is hard to conclude that the recommended dietary allowance for each nutrient should be reconsidered.