• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.4
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• pp.354-359
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• 2009

The productivity of ultra-small rotifer, Synchaeta kitina was investigated at the exchange rate of culture water (10, 20, 30, 40 and 50%) and initial inoculation densities (250, 600 and 900 inds. per mL) in semi-continuous culture. Also, the possibility of mass culture was investigated in a 100 L culture tank. Tetraselmis suecica was used as the feed for S. kitina in all experiments. The production of S. kitina increased with an increase in exchange rate of culture water. The highest production ($82.0{\times}10^5$ inds.) was achieved at 40% exchange rate of culture water. The production of S. kitina increased with an increase of initial inoculation density during the first week and the highest total production ($17.4{\times}10^6$ inds.) was achieved at 900 inds. per mL of initial inoculation density. However, on the second week, all treatments were not significantly different in total production (P>0.05). During the two weeks, total production of S. kitina at 900 inds. per mL of initial inoculation density was higher than at 600 inds. of initial inoculation density, but there was no significant difference (P>0.05). In the 100 L culture tank, density of S. kitina was kept from 516 to 890 inds. per mL and S. kitina was daily harvested $15.5{\times}10^6$ to $26.7{\times}10^6$ during the experimental period. The production cost for 100 million rotifers in semi-continuous culture was 63,656 won. The results from this study indicate that the optimal exchange rate of culture water and initial inoculation density for the semi-continuous culture of ultra-small rotifer, S. kitina are 40% and 600 inds. per mL, respectively.

• Korean journal of applied entomology
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• v.58 no.1
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• pp.49-54
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• 2019

Clover cyst nematode, Heterodera trifolii are one of the major pests that damage Chinese cabbage in high land cultivation area and have recently been distributed in Korea. This study was conducted to investigate the effect of temperature and inoculation density on the proliferation of clover cyst nematode. The clover cyst nematode was proliferated at $15-30^{\circ}C$ and the egg, female adult and cyst number was the highest at $20^{\circ}C$. As the inoculation density increased, the number of female cyst nematodes increased with the inoculation density and when 16 eggs were inoculated per gram of soil, the amount of growth, number of egg and cyst was the highest. Number of females and eggs were formed more in cabbage than in kale regardless of inoculation density. Chinese cabbage was a favorable host for clover cyst nematode growth than kale.

• Journal of the Korean Society of Tobacco Science
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• v.24 no.1
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• pp.27-31
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• 2002

Three kinds of inoculation methods, capillary, root cutting and dipping were compared for an efficient way to screening the resistant tobacco variety against bacterial wilt, Ralstonia solanacearum. The pricking a capillary tube contained the pathogenic bacterial suspension(10$^{7}$ cfu/$m\ell$) to an axillary bud of each tobacco plant showed different resistance well between varieties. The less period was required in inoculating work and in disease development for the inoculation method used with capillary tube than for two other inoculation methods tested also.

• Korean Journal of Plant Resources
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• v.11 no.3
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• pp.296-300
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• 1998

When medicinal mushroom, Poria cocos, is cultured , inoculation method of spawn is cross slice inoculation of which the both sides of pine tree were peeled and spawn of P.cocos was inoculated. However, this method required lots of inoculation amount. This study was carried out to improve the culturing method of P. cocos. A good growth of P.cocos was observed in MCM(mushroom complete medium), showing proper mycelia growth and density. In inoculation amount, conventional method(cross slice inoculation) requires 20 bottles of spawn. In contrast, short log method required 8 bottles of spawn and drilling inoculation method 2~3 bottles, which could save by 60% and 85-90% respectively. In the selectrion of tree species, pine and larch had better condition for spawn culture and sclerotia formation condition.In terms of yield , pine was 33.7kg/3.3$m^2$. In the yield of pine, conventional method was 23.4kg/3.3$m^2$, drilling inoculation 29.4kg/3.3$m^2$, short log inoculation 31.7kg/3.3$m^2$, therefore drilling inoculation could increase by 25% and short log inoculation 35%, In addition, management cost was also saved.

• Korean journal of applied entomology
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• v.56 no.4
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• pp.413-420
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• 2017

Sugarbeet cyst nematode (Heterodera schachtii) has recently been detected as a severe pest of Chinese cabbage fields in Korea. Two studies were performed to evaluate the effect of inoculation density of H. schachtii and plant age of Chinese cabbage on the final population of the nematodes. Chinese cabbage inoculated with high inoculation density (4 juveniles or eggs/g soil) showed significant differences in the number of cysts, females and eggs. The 40 day- old plants inoculated with high nematode densities yielded the highest nematode populations after 30 and 60 days of inoculation, compared with other plant age groups (0, 20, 30 day-old)used in the experiment. We, thus, summarize that, maximum nematode multiplication rates are made on comparatively older plants at the high nematode densities. Therefore, taking plant age into consideration at the transplanting of Chinese cabbage was recommended for the management of H. schachtii.

• Korean journal of applied entomology
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• v.12 no.4 s.17
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• pp.143-146
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• 1973

A greenhouse experiment was conducted to find out the influence of the inoculation density of Aphelenchoides besseyi on the growth of rice plant and the body length of female nematode. The rice plants showed reduced height, number of tillers and dry weight of plant when the nematode was inoculated. The body length of female nematode significantly decreased as the initial population density increased.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.3
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• pp.245-252
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• 1989

This experiment was carried out to study the effects of nitrogen concentration of cultural solution, rhizobium inoculation, and planting density on nodulation, contents of total-N and allantoin-N of the plants, and contents of crude fat and protein of the seeds with 4 cultivars and 1 non-nodulation isogenic line (Hwangeumkong, Jangbaegkong, Paldalkong, Clark, non-nodulating Clark) in nutri-culture. Modulation was increased significantly by the rhizobium inoculation and decreased by the increase of nitrogen concentration of the cultural solution and planting density, and more nodule per plant was observed in Hwangeumkong than other cultivars. Total nitrogen contents of the soybean plant were higher in Hwangeumkong and Janbaegkong compared with other two cultivars, and were increased by rhizobium inoculation and the increase of nitrogen concentration of the cultural solution except in case of $R_3$ stage of non-inoculation of rhizobium, and was decreased by the increase of planting density. Allantoin nitrogen contents of stem were lower in Paldalkong compared with other cultivars and was increased significantly by the rhizobium inoculation, and thus was correlated significantly with nodulation. Allantoin-N contents were also decreased by the increase of planting density and nitrogen concentration of the solution, and was very low in case of non-nodulating isogenic line at any level of nitrogen concentration. The contents of crude fat of the seed were lower in cases of higher nitrogen concentration of the solution and more plants per pot, but no effects of rhizobium inoculation was observed. The contents of crude protein were tend to increase by the increase of nitrogen concentration, but were little effected by rhizobium inoculation and planting density.

• The Korean Journal of Pesticide Science
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• v.18 no.3
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• pp.196-201
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• 2014

This study was conducted to develop economic injury level (EIL) of sweet potato whitefly, Bemisia tabaci, on oriental melon. In greenhouse, seedlings of oriental melon were transplanted at June 8, 2010 and we inoculated adult B. tabaci with the density of 0, 1, 5, 10, or 20 per ten leaves at July 14. Adult of B. tabaci increased approximately twenty five-fold at 60 days after inoculation in the plot of 20 adults per ten leaves. The damages on leaves and fruits by B. tabaci were started to appear at 20 days after inoculation, and the damage rates of leaves or fruits were 28.5 or 31.5 percent at 60 days after inoculation, respectively, in the plot of inoculation with 20 adults per ten leaves. The yield of oriental melon was reduced as the inoculation density of B. tabaci increased, and the relationship between inoculation density of B. tabaci and the rate of damaged fruit could be described by a linear regression Y = 0.961x + 0.0562 ($R^2$ = 0.976). Based on the relationship, the economic injury level was 5.1 adults of B. tabaci per leaf and the control threshold estimated by 80% level of economic injury level was 4.1 adults per leaf for control of sweet potato whitefly.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.289-292
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• 2002

The corneal tissue consists of three layers : epithelium, stroma, and endothelium. Central cornea is a highly differentiated tissue whereas the limbus contains the epithelial stem cell. In the present study. we report the engineering of the three-dimensional reconstructed cornea derived from rabbit limbal epithelial and stromal cells. The differentiation degree of corneal stem cells were assessed in serum concentration and inoculation density of stromal cells. Optimal condition differentiation of corneal stem cells is achieved when 5% FBS was supplemented to culture medium and $1-2{\times}10^5$ cells/ml inoculation density of stromal cells.

• Journal of Korean Society of Forest Science
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• v.101 no.1
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• pp.77-82
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• 2012

Sterilization of oak sawdust at $65^{\circ}C$ for Lentinula edodes bed cultivation can be efficient in sterilization facility cost, but its effect on the mushroom production is uncertain due to high contamination probability. The effective conditions for L. edodes hyphal growth in the low temperature sterilized oak sawdust were investigated with combinations of three sterilization temperatures ($65^{\circ}C$, $100^{\circ}C$ and $121^{\circ}C$) and four cultivation temperatures ($15^{\circ}C$, $20^{\circ}C$, $25^{\circ}C$, and $30^{\circ}C$). L. edodes inoculation density effect was also tested with 1 cm, 2 cm, and 4 cm distance in the sawdust (4%, 11% and 25% inoculation rate by surface area). L. edodes hyphal growth in the sawdust sterilized at $65^{\circ}C$ was as much as at those $100^{\circ}C$ and $121^{\circ}C$ when the fungus cultured below $25^{\circ}C$, but it was greatly reduced when cultured at $30^{\circ}C$. And the sawdust medium with 1cm distance inoculation density was fully occupied with L. edodes hyphae, but those with 2~4cm distance inoculation were contaminated by 4~33%. Therefore, we conclude that low temperature sterilized oak sawdust needs to be cultured under $25^{\circ}C$ after sufficient inoculation by 25% for successful bed cultivation of L. edodes.