• 한국초지조사료학회지
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• 제20권3호
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• pp.185-192
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• 2000

This experiment was canied out to determine the effect of management practices on the quality of round baled oat silage at experimental field of Grassland and Forage Crops division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiments are consist of randomized block design with 3 replications. The treatments are 3 wilting dates(0, 2 and 4 days), 3 wrap colors(white, black and green and 3 inoculant(untreated, Inoculant A and Inocuant B). The crude protein(CP) content was increased by prolonged wilting periods, but the effect of wrap color and inoculant were not founded. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) content of all silages were not founded significant difference, but in vitro dry matter digestibility of oat silage with inoculant was significantly higher compare with control. Wilting treatment increased the mean silage acidity compare with control and inoculant treatment significantly reduced silage acidity. Wrap color did not influence the silage acidity. Wilting or inoculant treatments increased lactic acid content but, decreased the content of acetic and butyric acid. The quality grade of all silage were grade 3, except inoculant treated silage. Wilting or inoculant decreased silage DM loss, but wrap color did not effect on siage DM loss. The result of this study indicate that wilting for 2-4 days and inoculant will improve the silage fermentation and quality of round baled oat silage. (Key words : Oat, Wilting, Inoculant, Wrap color, Round bale silage)

• 한국초지조사료학회지
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• 제26권3호
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• pp.139-146
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• 2006

본 시험은 젖산균 첨가제가 목초 라운드베일 랩핑 사일리지의 품질에 미치는 영향을 구명하기 위하여 2년간 수행되었다. 시험구 배치는 난괴법 3반복으로 무처리, 첨가제 A, B, C로 하여 총 4처리를 두었다. 조지방, 조단백질, in vitro 건물 소화율은 첨가제를 처리한 구에서 높게 나타났다. 또한 첨가제 처리는 사일리지의 pH를 유의적으로 낮추었다(p<0.05). 건물 함량은 첨가제 처리구가 높았으며 첨가제 처리로 유기산 함량은 유의적인 차이를 보여주었다(p<0.05). 젖산 함량은 첨가제 처리로 증가되었으나 초산과 낙산 함량은 감소되었다. 건물손실과 사일리지 품질은 젖산균 첨가제 처리로 개선되는 효과를 보여 주었다. 이상의 결과를 종합하여 볼 때 사일리지 조제시 미생물 첨가제의 이용은 사일리지의 발효와 품질을 개선시켜주는 것으로 나타났다.

• 한국주조공학회지
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• 제25권2호
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• pp.102-108
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• 2005

The ability and fading behavior of inoculant in ductile cast iron melt and the effect of minor element on them were investigated. The result obtanied on nodularization and the performance of inoculant were more distinct when the melt was treated and held at the high temperature range of $1450{\sim}1500^{\circ}C$ than at the lower one of $1350{\sim}1400^{\circ}C$. The performance of 5.2%Mg-Fe-Si alloy was the best of 4 nodularizers. That of Fe-75%Si(I) alloy was better than other 4 inoculants. The performance of the Fe-75%Si(I) inoculant was deteoriated by the addition of sulfur or bismuth. On the other hand, that was improved by the addition of cerium, even though its extent was not big.

• 한국작물학회지
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• 제51권6호
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• pp.534-538
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• 2006

The effects of Rhizobium inoculant, compost, and nitrogen on nodulation, growth, dry matter production, yield attributes, and yield of pea (Pisum sativum) var, IPSA Motorshuti-3 were assessed by a field experiment. Among the treatments Rhizobium inoculant alone performed best in recording number and dry weight of nodules/plant. The highest green seed yield of 8.38 ton/ha (36.9% increase over control) and mature seed yield of 2.97 ton/ha (73.7% increase over control) were obtained by the application of 90 kg N/ha. The effects of 60 kg N/ha, Rhizobium inoculant alone and Rhizobium inoculant along with 5 ton compost/ha were same as the effect of 90 kg N/ha in recording plant height, root length, dry weight of shoot, and root both at preflowering and pod filling stages, number of mature pods/plant, number of mature seeds/pod, 1000-seed weight, green, and mature seed yields of pea.

• 한국환경과학회지
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• 제27권5호
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• pp.333-340
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• 2018

This study aimed to estimate the effect of inoculant application level on chemical composition and bacterial count of fermented chestnut meal (FCM), and its rumen fermentation characteristics. The inoculant contained Lactobacillus acidophilus ($1.2{\times}10^{10}cfu/g$), Bacillus subtilis ($2.1{\times}10^{10}cfu/g$), and Saccharomyces cerevisiae ($2.3{\times}10^{10}cfu/g$). The chestnut meal mixed with molasses, double distilled water, and inoculant at 1 kg, 3 g, 480 mL, and 20 mL ratio for the basal chestnut meal diet. The double distilled water from basal chestnut meal diet was substituted with bacterial inoculant at a level of 0 (Control), 20 (Medium), and 40 mL (High) in the experimental diets. The mixed experimental diets were incubated at $39^{\circ}C$ for 7, 14, and 21 days, respectively. On 7 days of FCM incubation, the contents of crude protein (CP) (quadratic, P=0.043) and neutral detergent fiber (quadratic, P=0.071) decreased by increases of inoculant application levels, whereas bacterial count (quadratic, P=0.065) and rumen $NH_3-N$ (linear, P=0.063) increased. By increases of inoculant application levels on 14 days of FCM incubation, the increases were found on dry matter (DM) (quadratic, P=0.085), CP (quadratic, P=0.059), acid detergent fiber (quadratic, P=0.056), in vitro DM digestibility (linear, P=0.002), rumen total volatile fatty acid (VFA) (linear, P=0.057), and rumen iso-butyrate (linear, P=0.054). However, the decreases were found on bacterial count (linear, P=0.002), propionate (linear, P=0.099), and butyrate (quadratic, P=0.082). On 21 days of FCM incubation, in vitro DM digestibility (linear, P=0.002) and total VFA (linear, P=0.001) increased by increases of inoculant application levels, whereas the contents of CP (quadratic, P=0.034) and neutral detergent fiber (quadratic, P=0.047) decreased. These results indicate that the FCM with a medium level of inoculant application and 14 of fermentation had beneficial effects by increasing DM digestibility and rumen total VFA content, without altering bacterial count.

• Asian-Australasian Journal of Animal Sciences
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• 제24권3호
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• pp.369-378
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• 2011

The effect of a homolactic inoculant containing a blend of Lactobacillus plantarum, Pediococcus acidilactici and Enterococcus faecium or, the anionic surfactant, sodium dodecyl sulphate (SDS), alone or in combination on fermentation characteristics, aerobic stability and in situ DM, OM and NDF degradability of barley silage was investigated. Barley (Hordeum vulgare, L.) was harvested (45% DM), chopped and treated with water at 24 ml/kg forage (Control), inoculant at $1.09{\times}10^5$ cfu/g forage (I), SDS at 0.125% (wt/wt) of forage (S) or with the inoculant ($1.09{\times}10^5$ cfu/g) plus SDS (0.125% wt/wt; I+S). The treated forages were ensiled in triplicate mini silos and opened for chemical and microbiological analyses on d 1, 2, 3, 7, 14, 42 and 77. Silage samples from d 77 were opened and aerobically exposed for 7 d. The in situ rumen degradability characteristics of silage DM, OM and NDF were also determined. The terminal concentration of NDF in S and I+S was lower (p<0.001) than in other treatments. Lactate concentration was higher (p<0.001) and the rate and extent of pH decline were greater (p<0.001) in I and I+S than S and Control silages. A homolactic pathway of fermentation in I and I+S was evidenced by reduced (p<0.001) water-soluble carbohydrates concentration, higher lactate (p<0.01), lower acetate (p<0.01) and lower pH values (p<0.001) than in S and Control silages. All silages remained stable over 7 d of exposure to air as indicated by lower temperatures and moulds, and by non-detectable yeast populations. The treated silages had lower DM and OM degradability than in the Control but NDF degradation characteristics of I+S were improved compared to other treatments. It is concluded that the inoculant alone improved the fermentation characteristics whereas the combination of the inoculant with SDS improved both fermentation and NDF degradability of barley silage.

• 한국주조공학회지
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• 제24권5호
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• pp.295-303
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• 2004

The effects of three minor elements, S, Ce and Bi, on the inoculation ability and fading behavior of 1.0 wt%Ba-Fe-Si were investigated through thermal analysis. The performance of 1.0 wt%Ba-Fe-Si inoculant was better and more consistant at the high temperature range of $1,450{\sim}1,500^{\circ}C$ than that of low one of $1,300{\sim}1,400^{\circ}C$. That was improved with individual addition of three minor elements. The optimum amount of addition was 1.0% of the weight of inoculant added, respectively. That was improved also by the simultaneous addition of two or three kinds of minor elements. Even though worse with the addition of two kinds of them simultaneously than with individual addition, that was improved with the simultaneous addition of all three kinds over that with the individual one.

• 한국작물학회지
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• 제50권2호
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• pp.112-119
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• 2005

The effects of Rhizobium inoculant, nitrogen, phosphorus, and molybdenum on nodulation, dry matter production, yield attributes, pod and seed yields, protein and phosphorus contents in seed of pea (pisum sativum) var. IPSA Motorshuti-3 were assessed by a field experiment. Among the treatments Rhizobium inoculant in combination with 25kg P and 1.5kg Mo/ha performed best in recording number of nodules/plant, total dry matter yield, number of pods/plant, number of seeds/pod, 1000-seed weight, green pod yield, green and mature seed yields of pea. The highest green pod yield of 15.37 t/ha ($97.05\%$ increase over control) and green seed yield of 9.6t/ha ($69.31\%$ increase over control) were obtained by inoculating pea with Rhizobium inoculant in association with 25kg P and 1.5 Mo/ha. The effects of 60 or 120kg N/ha were comparable to Rhizobium inoculant in most cases. There were positive correlations among yield attributes, yield, protein and phosphorus contents in seeds of pea. From the viewpoint of yield attributes, yield, and seed quality, application of Rhizobium inoculant along with 25kg P and 1.5kg Mo/ha was considered to be the balanced combination of nutrients for achieving the maximum output from cultivation of pea in Shallow-Red Brown Terrace Soil of Bangladesh.

• 한국초지조사료학회지
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• 제19권2호
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• pp.115-120
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• 1999

본 시험은 첨가제 처리가 알팔파 사일리지의 품질에 미치는 영향을 구명하기 위하여 1996년 6월부터 12월까지 축산기술연구소 초지사료과에서 개화중기에 수확된 알팔파(Vernal)를 사일리지로 조제시 무처리, 개미산, 당밀 및 젖산균 제제 (inoculant A 및 inoculant B)등 5처리 3반복의 난괴법 배치로 수행하였으며 그 결과는 다음과 같다. 첨가제 처리로 알팔파 사일리지의 조단백질 및 NFE 함량은 대조구에 비해 높게 나타났고, 조회분 및 조섬유 함량은 대조구에서 11.5 및 39.6%로 높았다. ADF 및 NDF 함량은 대조구에서 36.2 및 48.6%로 첨가제 처리구보다 높았으며 사일리지 조제전에 비해 약 4% 증가하였다. 사일리지의 pH는 대조구에서 5.45로 가장 높았고 개미산 및 inoculant A 처리구에서 4.35 및 4.32로 유의적으로 낮게 나타났다. 또한 건물 함량은 대조구에서 유의적으로 낮았으며 건물손실률은 대조구에서 높았으며 첨가제 처리구간에는 유의적인 차이가 없었다. 암모니아태 질소 함량은 개미산 처리구에서 가장 낮았고 젖산균수는 무처리 및 개미산 처리구에서 낮은 경향을 보였다. 첨가제 처리에 따른 유기산 조성에 있어서는 inoculant A 및 inoculant B 처리구에서 총산 및 젖산 함량이 높게 나타났으며 품질등급에 있어서도 젖산균제 처리구가 가장 높았다. 이상의 결과를 종합하여 볼 때 고품질의 알팔파 사일리지를 조제할때는 첨가제 처리가 효과적이며 안전성, 기계부식 문제를 고려할 때 살포가 용이한 젖산균제의 이용이 바람직한 것으로 생각된다.

• 한국초지조사료학회지
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• 제35권4호
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• pp.283-289
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• 2015

수확시 숙기와 조제방법이 호밀 라운드베일 사일리지의 건물 손실률과 형태변화에 대한 영향을 구명하기 위하여 3가지 시험을 수행하였다. 호밀은 3번의 생육단계에 맞춰 수확하였으며 (수잉기, 출수기 및 개화기) i) 예건 (비예건, 단기예건, 장기예건)에 따른 변화, ii) 유산균첨가제 (무처리, 첨가제 A 및 첨가제 B) 처리에 따른 변화, iii) 비닐색 (백색, 흑색, 녹색)에 따른 변화를 관찰하였다. 수잉기 및 비예건 처리구에서 저장 2개월 후의 형태 변화가 심하게 나타났다. 그러나 출수기 이후의 수확 처리구에서는 변화정도가 심하지 않았다. 첨가제 처리는 사일리지의 형태변화와 건물 손실률을 감소시켰다. 건물 손실률은 수확이 지연되거나 첨가제 처리로 유의적으로 감소되었다. 수잉기 처리에서는 형태변화가 심하게 관찰이 되었는데 첨가제 처리구가 비 처리구보다 형태변화가 더 심하게 일어났다. 흑색비닐 처리는 다른 비닐색에 비해 형태변화가 심하게 일어났 으나 건물 손실률은 비닐색에 따른 차이가 없었다. 상관관계에서는 수확시 숙기와 예건기간이 형태변화와 정의 상관이 있는 것으로 나타났다. 이상의 결과를 종합하여 볼 때 건물함량은 사일리지의 형태변화에 영향을 주는 가장 큰 요인으로 나타났다.