• Journal of Animal Science and Technology
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• v.65 no.2
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• pp.401-411
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• 2023

Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be derived using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In this study, the iTRAQ-labeling system was coupled with liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify differentially expressed CM10-fractionated proteins between fresh and frozen-thawed boar semen. A total of 76 protein types were identified to be differentially expressed, among which 9 and 67 proteins showed higher and lower expression in frozen-thawed than in fresh sperm samples, respectively. The classified functions of these proteins included oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes, which are involved in adenosine triphosphate (ATP) synthesis; and sperm flagellum and motile cilium, which are involved in sperm tail structure. These results suggest a possible network of biomarkers associated with survival after the cryopreservation of Duroc boar semen.

• Journal of Veterinary Clinics
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• v.39 no.6
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• pp.319-325
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• 2022

Two-port laparoscopic ovariectomy (Lap-OVE) has been performed in small dogs, using 3-mm and 5-mm portal sites, and is associated with reduced surgical stress and postoperative pain. However, extension of the incision is often needed to extract the ovaries. In this study, we aimed to minimize invasiveness by using smaller-sized cannulas as well as a novel technique for ovary extraction. Lap-OVE was performed on six, healthy female dogs (range, 3 to 7.2 kg) using two 3-mm midline portals. The middle finger of a size M nitrile glove was cut at its base and sterilized preoperatively. The ovary was suspended at the body wall using a 1-0 blue nylon needle, and the ovarian pedicle and ligaments were transected using a 3-mm bipolar forceps. To facilitate the glove passing through the 3.9-mm port, it was turned inside out to expose the smooth inner surface, before being inserted into the abdominal cavity with an applicator. Both ovaries were placed inside, and the mouth of the glove was exteriorized through the port with a laparoscopic grasping forceps. The ovaries were morcellated inside the glove, using Adison-Brown tissue forceps and iris scissors, which enabled safe extraction without incision enlargement. Median incision lengths were 4.3 mm (3.5-mm cranial cannula) and 4.8 mm (3.9-mm caudal cannula). An advantage of this procedure was that there was no need for skin sutures. In conclusion, using our novel technique, sutureless Lap-OVE was possible in small dogs using two 3-mm portal sites without additional incision.

• Nuclear Engineering and Technology
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• v.55 no.5
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• pp.1855-1862
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• 2023

Graphite crucibles are used for melting uranium and its alloys in VIM furnace. Various coating materials namely Al₂O₃, ZrO₂, MgO etc. are applied on the inner surface of the crucibles using paint brush or thermal spray technique to mitigate U-C interaction. These leads to significant amount of carbon pick-up in uranium. In this study, the attempts are made to develop multilayer coatings comprising of SiC/Y₂O₃ and Mo/Y₂O₃ on graphite to study the feasibility of minimizing U-C interaction. The parameters are optimized to prepare SiC coating of about 70㎛ thickness using CVD technique on graphite coupons and subsequently Y₂O₃ coating of about 250㎛ thickness using plasma spray technique. Molybdenum and Y₂O₃ layers were deposited using plasma spray technique with 70㎛ and 250㎛ thickness, respectively. Interaction studies of the coated graphite with molten uranium at 1450℃ for 20 min revealed that Y₂O₃ coating with SiC interlayer provides physical barrier for uranium-graphite interaction, however, this led to the physical separation of coating layer. Y₂O₃ coating with Mo interlayer provided superior barrier effect showing no degradation and the coatings remained intact after interaction tests. Therefore, the Mo/Y₂O₃ coating was found to be a promising solution for minimizing carbon pick-up during uranium/uranium alloy melting.

• Journal of the Korean Society for Heat Treatment
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• v.36 no.6
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• pp.351-358
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• 2023

Bearings are mechanical components that support loads and transmit rotation. The inner and outer rings come into contact with the rotating mechanism, requiring a very high level of hardness. To meet this requirement, heat treatment is commonly performed. The heat treatment process inherently involves thermal deformation. Particularly in the case of large bearings, significant deformation relative to the bearing's shape can occur, making accurate deformation prediction during heat treatment essential. However, predicting deformation in heat treatment is challenging due to the simultaneous consideration of phase transformation, heat transfer, and bearing deformation. In this study, an analysis of heat treatment-induced deformation in bearings was conducted, taking phase transformation into account. The thermal and mechanical properties were calculated based on the chemical composition of the bearing material. This information was then used to perform a deformation-heat transfer-phase transformation analysis. To validate the reliability of the analysis, experiments were conducted under the same conditions. When comparing the analysis and experimental results, differences in deformation were observed. These differences were attributed to variations in phase transformation conditions between the analysis and experiments. Consequently, it is anticipated that supplementing these results will enable the prediction of deformation while considering phase transformation conditions in bearings.

• Journal of Fashion Business
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• v.27 no.5
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• pp.93-107
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• 2023

This study aimed to design a knee brace with dry electrode EMS (Electrical Muscle Stimulation) for elite badminton players suffering from knee pain and assess its effectiveness in relieving pain and improving mobility. The assessment measured knee joint range of motion (ROM), Sargent jump height, and pain perception using a visual analog scale (VAS). Four experimental groups were established: stability, pain induction after 100 squats, muscle soreness induction with a regular knee brace, and muscle soreness induction with the EMS knee brace. The most suitable knee brace was selected from four samples to design the EMS knee brace. The conductive fabric was integrated into the inner surface of the knee brace to enhance EMS conductivity for the quadriceps muscles. Tensile strength tests showed that the dry electrode did not significantly affect the physical functionality of the knee brace.Regarding knee joint ROM and Sargent jump height, the EMS knee brace outperformed muscle soreness induction with a regular knee brace and wearing a standard knee brace. VAS measurements demonstrated that the EMS braces effectively alleviated pain perception in most cases. The results indicate the potential for developing EMS braces to alleviate pain and prevent injuries for athletes across various sports.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.36 no.1
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• pp.1-10
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• 2024

As increasing demands for a floating structure expanded from offshore industry facilities to living facilities, it has emerged that necessity of techniques to reduce motions of a floating structure. This study present a floating structure with porosity on the outer surface of the floater. Under each regular and irregular wave, responses of the floater was investigated in frequency domain. The proposed structure is composed of inner and outer floaters, which are connected to each other and the outer wall is perforated, and the heave and the pitch of floaters with different perforation rates (0~30%) were compared with at both the center and the edge. The results showed that pitch responses can be decreased by increasing of perforation rate of the floater. Comparing with responses of the non-perforated floater, those of the proposed floating structure were reduced to above 10% and 2%, respectively for regular and irregular wave conditions.

• Journal of the Korean Society of Radiology
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• v.18 no.2
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• pp.83-91
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• 2024

The purpose of this study was to determine the doppler fluid effects of carbonated water (CBW) in a bi-directional flow phantom model. A bi-directional flow phantom model was chosen to realize arterial and venous flow, and the structure of the inner and outer tanks allowed for fluid circulation and also made the size of the phantom small. Carbonated water (CBW), salt fluid (SAF), sugar fluid (SUF), and distilled water (DW) were used as fluids, and ultrasound scans were performed at depths of 1.5 cm and 3.0 cm from the surface of the tank, using B-mode and color Doppler effects. All fluids tested showed color Doppler effects, but CBW had the highest doppler shift and the least variation with depth. In conclusion, we determined that CBW was the most suitable fluid to be used as a doppler fluid and confirmed that the bubbles dissolved in CBW act as doppler scatterers, just like red blood cells inside human blood. Therefore, it is possible that CBW can be used as a blood-mimicking fluid in doppler ultrasound phantoms through further research, and this study will provide basic data.

• Anatomy and Cell Biology
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• v.56 no.2
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• pp.259-267
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• 2023

Stature is an essential component of biological profile analysis since it determines an individual's physical identity. Long bone dimensions are generally used to estimate the stature of skeletal remains; however, non-long bones such as the sternum, cranium, and sacrum may be necessary for some forensic situations. This study aimed to generate a regression equation for stature estimation of the skeletal remains in the Thai population. Ten measurements of the sacrum were measured from 200 dry sacra. The results revealed that the maximum anterior breadth (MAB) provided the most accurate stature prediction model among males (correlation coefficient [r]=0.53), standard error of estimation (SEE=5.94 cm), and females (r=0.48, SEE=6.34 cm). For the multiple regression model, the best multiple regression models were stature equals 41.2+0.374 (right auricular surface height [RASH])+1.072 (anterior-posterior outer diameter of S₁ vertebra corpus [APOD])+0.256 (dorsal height [DH])+0.417 (transverse inner diameter of S₁ vertebra corpus [TranID])+0.2 (MAB) with a SEE of 6.42 cm for combined sex. For males, stature equals 63.639+0.478 (MAB)+0.299 (DH)+0.508 (APOD) with a SEE of 5.35, and stature equals 75.181+0.362 (MAB)+0.441 (RASH)+0.132 (maximum anterior height [MAH]) with a SEE of 5.88 cm for females. This study suggests that regression equations derived from the sacrum can be used to estimate the stature of the Thai population, especially when a long bone is unavailable.

• Journal of Radiation Industry
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• v.17 no.2
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• pp.199-207
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• 2023

Since seeds can be directly used as food resources as well as for crop cultivation or preservation of genetic resources, it is essential to develop high-quality seed processing technology to increase agricultural productivity. Seed treatment means processing technologies of seeds through physical or chemical treatment processes from after harvesting seeds to before sowing of seeds to improve germination and growth rate, durability, and immunity, etc. Since chemical seed treatment technology using pesticides or plant growth regulators has problems of environmental pollution and human toxicity, it is desired to develop an alternative technology. As a physical seed treatment method, various technologies such as ionizing radiation, plasma, microwave, and magnetic field are being developed, and some of them are being used practically. In this paper, I will summarize the mechanism of seed priming and disinfection, and the advantages and disadvantages of application, focusing on these physical seed treatment methods. Low dose or moderate intensity ionizing radiation, microwave, low-temperature plasma, and magnetic field treatments often promoted seed germination and seedling growth. However, effective removal of direct seed pathogens at these treatment intensities appears to be difficult. And it has been shown that relatively high-dose electron beam treatment using low-energy electron beams kills microorganisms on the seed surface and hull layer while not damaging the inner tissue of the seed, and is also effectively used for seed treatment on a commercial scale. In order to put the physical seed treatment technology to practical use in Korea, it is necessary to develop an economical scale treatment device along with the development of individual treatment technology to each crop.

• ALGAE
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• v.32 no.2
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• pp.101-130
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• 2017

The ichthyotoxic Cochlodinium polykrikoides red tides have caused great economic losses in the aquaculture industry in the waters of Korea and other countries. Predicting outbreak of C. polykrikoides red tides 1-2 weeks in advance is a critical step in minimizing losses. In the South Sea of Korea, large C. polykrikoides red tide patches have often been recorded offshore and transported to nearshore waters. To explore the processes of offshore C. polykrikoides red tides, temporal variations in 3-dimensional (3-D) distributions of red tide organisms and environmental parameters were investigated by analyzing 4,432 water samples collected from 2-5 depths of 60 stations in the South Sea, Korea 16 times from May to Nov, 2014. In the study area, the vegetative cells of C. polykrikoides were found as early as May 7, but C. polykrikoides red tide patches were observed from Aug 21 until Oct 9. Cochlodinium red tides occurred in both inner and outer stations. Prior to the occurrence of large C. polykrikoides red tides, the phototrophic dinoflagellates Prorocentrum donghaiense (Jun 12 to Jul 11), Ceratium furca (Jul 11 to Aug 21), and Alexandrium fraterculus (Aug 21) formed red tides in sequence, and diatom red tides formed 2-3 times without a certain distinct pattern. The temperature for the optimal growth of these four red tide dinoflagellates is known to be similar. Thus, the sequence of the maximum growth rates of P. donghaiense > C. furca > A. fraterculus > C. polykrikoides may be partially responsible for this sequence of red tides in the inner stations following high nutrients input in the surface waters because of heavy rains. Furthermore, Cochlodinium red tides formed and persisted at the outer stations when $NO_3$ concentrations of the surface waters were < $2{\mu}M$ and thermocline depths were >20 m with the retreat of deep cold waters, and the abundance of the competing red-tide species was relatively low. The sequence of the maximum swimming speeds and thus potential reachable depths of C. polykrikoides > A. fraterculus > C. furca > P. donghaiense may be responsible for the large C. polykrikoides red tides after the small blooms of the other dinoflagellates. Thus, C. polykrikoides is likely to outgrow over the competitors at the outer stations by descending to depths >20 m and taking nutrients up from deep cold waters. Thus, to predict the process of Cochlodinium red tides in the study area, temporal variations in 3-D distributions of red tide organisms and environmental parameters showing major nutrient sources, formation and depth of thermoclines, intrusion and retreat of deep cold waters, and the abundance of competing red tide species should be well understood.